Simple sequence repeat (SSR)
>UN27222
GCGTTGCCCTTTGCGTGATGAAAAACCTGTCTACAAAGATGTTAAAGGCTCTGGTGGCTTGGTTCTGAGCATTGTTGAAATTGAAAAGCTGAGA
CTGAAGCGAGAAGGGGAAGACAGGATGACACGAGCTCGACTAGAGAGACAGATCGAGGAGTTTGGAAATGATTGTCTTAAAGAATTTCAAGAGC
AAATGCTTGCAGTGGAGTTGCTAAACGAGAGATTGATCGTGGTTGAAAATGAAGTGAAGATCTGTAGAGAGACACTTTCTGAAAGCAAGAATGA
TGAGACTTCTGAAGTTGCTGCTACTGCTGCTACTGCTGCTGCTGCTGCTATGGAAGAGACATGAGATGCTCTTAAGGTTTGTGCAATTAGTGAA
GCTTTTGAAGATCGTTCCAACAGATATTATATTAGCTTCGTTTTGTATGATGTAGACGATGTTGATTGAATCTCTTTAATCTCGTTCTAGGAAA
TGTAATCAACGGTTTTGATCAGTAAGAGTCTTAATTAGTTAC |
Primers for UN27222 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGCAGTGGAGTTGCTAAACG |
60.05 |
20 |
TTGGAACGATCTTCAAAAGC |
57.944 |
20 |
201 |
196 |
396 |
| 2 |
TCAAGAGCAAATGCTTGCAG |
60.28 |
20 |
TTGGAACGATCTTCAAAAGC |
57.944 |
20 |
216 |
181 |
396 |
| 3 |
TGCAGTGGAGTTGCTAAACG |
60.05 |
20 |
TTGCACAAACCTTAAGAGCATC |
59.406 |
22 |
173 |
196 |
368 |
|
|
