 |
Simple sequence repeat (SSR)
>UN28014
GGAAAGAAACATAAACCAAAGCTAAAGATACATAATTTCTCGATCTTTGGAGGGAGCCGTCAGATCAGAATCTGATCATGGACAAGGTGATGAG
AATGTCATCAGAGAAAGGAGTTGTGATCTTCACCAAAGACTCTTGTTGTCTTTGCTACGCCGTGCAGATCCTTTTCCGTGACCTTAGGGTTCAA
CCAACCATCCACGAGATCGACAATGATCCTGACTGCCGCGAGATAGAGAAGGCCTTAGTCCGTCTTGGCTGCACCAACGCTGTTCCTGCTGTTT
TCGTCAGTGGTAAGCTGGTGGGTTCGACCAACGAAGTCATGTCGCTTCACCTAAGTGGCTCTCTTGTTCCATTGATCAAGCCGTATCAGTCATT
TCATAACTAGAAAATGAATGGATCCTTAAACAAATGATAATTGCTATGTTGATTAGAAAGTAAATAATGATGGAAATATTACACGTAAATGTGT
ATGACGTTCTTATATATATATAAGTTACTTTTTATATTTTTTTTTTGTAGCTGATCATGTCTAAGAAGTTTATAACTAGCTAGATTCGTATTTC
CCTGCTTAATCAATCAGTGGATGAACTGATGTGCTATCTAATGTAATGGAGAAAATTATATATCATATGTTTGTATTGCCTTTGGTTTTAATGT
ATGTCTCTGGTAGATACTATTATCAGACTTTCTGG |
Primers for UN28014 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGTTCGACCAACGAAGTCAT |
59.973 |
20 |
GCAGGGAAATACGAATCTAGC |
57.987 |
21 |
267 |
303 |
569 |
| 2 |
GGTTCGACCAACGAAGTCAT |
59.973 |
20 |
TTGATTAAGCAGGGAAATACGAA |
59.98 |
23 |
275 |
303 |
577 |
| 3 |
GGTTCGACCAACGAAGTCAT |
59.973 |
20 |
AGCAGGGAAATACGAATCTAGC |
58.934 |
22 |
268 |
303 |
570 |
|
|
