 |
Simple sequence repeat (SSR)
>UN00316
TTCACGTAAGAAAGTCATACATTCACAAAAGAACAGCATTGTTAAGTGTGCTTAACAAAAGAGTATAAATCCCAAAGACAAAAAAATCTAAGAG
GGGAGGGGGCGCGCATTAAAGCTCTGTGGTTTGCTTAGTAACTAAAATCTTCTGGCTCTAGCCCATGGTGGCAACCTTGGAGCTTGCACTTTAC
CCCCACTGTTGTTGTTGTTGTTACTACTATTCCTCTGCTCTTTCATGATTGCAAACCGAGAGTCCAGTGTCTTCCACTTCCCTCCTCCATTTTC
TATTTTCTGTTCCACCTCATGCCTCTTTAATCTAGCGTGTAAAGATCTATTCCGGACTGGTGGAGCAAGAAGCCTCGACTGATTTGCACTAGTC
ATCCTTCCAGCATTGAAAGCTCTTCTACGGACACTGAGTGGAGGACCAGTAGCGGCTTTATGAGCAACGTTTGTTGCAACAGGAAACTGATTTC
CTTTGAAGTTAGACCTTGTCTTAGCAACAGCACCCTGTCTAACTCCAGAAAGTGAGTTCACGTAATGCTTCTCTTTACAGGTATTATTATTTCT
CGCAGCACCATTGAAGTTTCGGTTTTTGTTCCTGGCTCTCCTTGAACGTTTGCCCCCCTTGTGCCCTTTGCTATTCCTCTTTGACAACTTGATG
AGATCATCCAAAGCCATCGCAACTTTCTTTTCGTTGAGAGCAATGAGATCAGCCACAACTTGTGTTTCACTCTCCATCTTTTCACAGTCAGTGA
AGTAAAAGCTAAAAAAAAGGCGATCAGGAGATACGAAGAAGAAGACGACGAAACGAAGATTAAGAGTCTTCCTGAACAGAGACGATGACCGAGT
CAACTCCC |
Primers for UN00316 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TTGGAGCTTGCACTTTACCC |
60.249 |
20 |
GCTCATAAAGCCGCTACTGG |
60.003 |
20 |
271 |
171 |
441 |
| 2 |
TTGGAGCTTGCACTTTACCC |
60.249 |
20 |
AGTGCAAATCAGTCGAGGCT |
60.02 |
20 |
202 |
171 |
372 |
| 3 |
TTGGAGCTTGCACTTTACCC |
60.249 |
20 |
AGCTTTCAATGCTGGAAGGA |
59.955 |
20 |
227 |
171 |
397 |
|
|
