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Simple sequence repeat (SSR)
>UN30434
GGGGAAAAAAGATTATTCCATTGAACTTTATATCATCCAAATTTAATTCCACTGTACAATTTTTTTTTTTAATTTGACCACAACCCGAAACTGA
ATTGCTCTTCAAATCTCCAAATCGATGACTAAAAACTCTCTCTGTCACATGCCAAAAACTACTCGACCTTGGGTAATAATCCACTATCCCTGAA
ACCGCCTTTACCGGTTCCGTATAAACCTCCTCCACCTCCTCCGACAGCGAATCCACCACTATCTTTCTGAATCTCCGACATATAACTCCTCACT
TCCGCCTTAATCATCTCCTGCACCACCCTCATAAACTCATCTCTACCCGCCGCCTCTACACTATCAATCCTCATCTGACTCTCAAACCGCTGAA
ACAGCAACGCGTTGTTGCTGCTCGAACCCGTGTTCCTTGACCCCTTGCCGTCATCAGCTCCGGGAAGTGACAGCGACAGACTCAACGACGTTGC
CGGATCCTCCTCCTCCGACGGCACCACCGTGAATCCGCCGGAAACCGGCATCGGAGACGGCGGAAATGGAATCGTGCTGGTGGGACTCTCAGGG
CTAATCAAGTACAGCTCAGTGTCCACCGGAGGAGAAGACTCAGAGCTCACCGATCTCCTCTTCGCGACGTGATCACAGTCTCCTCCTCCTCCTC
CTCCTCCGCCGGGTAAGAACTTTCGCTTGAGCGTGGAGTTCCAGTGGTTCTTCACGGCGTTGTCCGTGCGCCCGTTG |
Primers for UN30434 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CACCGGAGGAGAAGACTCAG |
59.978 |
20 |
ACTGGAACTCCACGCTCAAG |
60.444 |
20 |
116 |
588 |
703 |
| 2 |
CTCAGAGCTCACCGATCTCC |
60.096 |
20 |
ACTGGAACTCCACGCTCAAG |
60.444 |
20 |
101 |
603 |
703 |
| 3 |
TCAGAGCTCACCGATCTCCT |
60.096 |
20 |
ACTGGAACTCCACGCTCAAG |
60.444 |
20 |
100 |
604 |
703 |
|
|
