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Simple sequence repeat (SSR)
>UN32821
GAATTCCCTCTTTTTAAAATACCAAAAACATTATTTTCCAAACATTTCTGTAACATCCCAATTCCGGGTTTGGATTTTTTTTTAGTATAACGGG
CCTCCCTACGGTCCATTTGACATAAATCTAGGGTTCTCTACAGATTTTTATAAATAGAACTGCAGCCCCTCCTTCTTTCCTCATCACAAAATTT
GCAGCAGAGCTCTGCAGAAATTTCTGGAAATTGGAAGTTCTAGCCGTCGCCTCCCTTCTCTCTCCCGCGCCGTCTCTCTCCTCTCTCCCTCCTC
GCCATCCGATCTCTCTCTCTCTCACCTCGGCGCTCTCTCTCTCCTTGCCGGCGGTCGATCGGTGGTGGTGGTGGTGATCGCCGTGTGGTGTGGT
GGTGTGGTGGTGACCGGATCAGATCTTGTTTTCCCCTTTGCCTCTTGTTCCAAATCCAGATCCAGATCTAGATTAAGGTGAGGTGTCTTGAACC
CATCTTGCTCCCTTGAACTCTATATTGCCTTATGTTGGATCTAGGATTAATTAGTCTTTGTTTGAAAGTTAAAGTGTTAGTTCATGATGAAAGT
TAGGGTGTTAGATGGATGGATAATACTACATAAGAACCTTCACACTGTTAAATGAGACTTAATGGACTGCTTTGTATTGATGTGGCTAATGATT
GTGTGATTGACTTGTATCCCATGTGATGTTATGTGATTAAAAGCTACGTTGCTAGAGAAAAGTGTATGCTCTGGTGATC |
Primers for UN32821 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CCCCTCCTTCTTTCCTCATC |
60.008 |
20 |
AGATCTGATCCGGTCACCAC |
59.928 |
20 |
243 |
160 |
402 |
| 2 |
ATAGAACTGCAGCCCCTCCT |
60.233 |
20 |
AGATCTGATCCGGTCACCAC |
59.928 |
20 |
255 |
148 |
402 |
| 3 |
CCCCTCCTTCTTTCCTCATC |
60.008 |
20 |
GGCAAAGGGGAAAACAAGAT |
60.299 |
20 |
259 |
160 |
418 |
|
|
