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Simple sequence repeat (SSR)

>UN35009
GACGACGTAGAGCAAACTGTGGTCAAGGGAGATTCGAATAAACGATCCGTTTAGAGCTCCGATTCGAGCTAACAAGCGAGAAGAACTGAGATTC
GATTCGACTCTAGGCGATGAACGGTAGACAGAGATCTGTCGCAGGAGCAGCGGCCGTGCACCACCAGAGGCAGCTCTCCGATAACCTTCTCGAC
ATGTCTTCCTCCAATGGCAGCAGATGGCTCCAACAATCCTCCTCTGGTCTCCCCCACTTTCATCCCTCCCCCAATGATTATGGATACTATGCTG
GTGGTGGTGGTGG
ACAAGCAGCAGCAACAAGAGGATACCATCAGAGAGGAAACGACTTCTTTGGAGAACCCACTACTCCTCAGTACGCTTCTCG
TCCTTCCACCCAAAGAAAGAACAACACTGATGAATCCGAGTTTAGTCCTGGGCTCTTAGATCTACATTCTTTTGATACTGAACTTCTCCCCGAG
ATTCCGGTCTCTAGCCAGTTAGATGGCTCCTCAATGTTCAATCCGAGTCGAAGCCAAAGCTTTGAGGACTTTGAGGCTTACAACAAACAGCCTA
ACCGTAGTAGTCGCCTGTTGGCTGATAACTTGGCTGCTGAGAAAGAAAGGATGAATGCTGTTGCGAAGATCAGAGTTGTTGTGCGTAAGAGACC
ACTTAACAAAAAGGAGTCGTCAAAAACGAGGAAGACATTGTTGACACACATTCCATTGCTTAACTGTTCATGAAACCAAACTTAAAGTTGACTT
AACAGCCTATGTTGAAAGCATGAATTTGTGTTTGATGCCGTGCTAGATGAGAAGTATCAAATGATGAGTTTATCGTGAGACAGTG

Primers for UN35009 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 ACATGTCTTCCTCCAATGGC 59.934 20 GGACGAGAAGCGTACTGAGG 60.012 20 193 187 379
2 CAGATGGCTCCAACAATCCT 60.073 20 GGACGAGAAGCGTACTGAGG 60.012 20 171 209 379
3 AGGCGATGAACGGTAGACAG 60.277 20 GGACGAGAAGCGTACTGAGG 60.012 20 274 106 379