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Simple sequence repeat (SSR)
>UN35322
GACAGACTAAAAGTAAACAACACGAAATTATTTGGGGGAAACATAAATTGAGTTCAATAAGAAGAATACACAACAGGCCCAATCTATTGGGCCC
AATAAAACTAGGGCCTAAAAGAGGAGCCACTGTGGCTTCTCTTACCACCCTCCTCTGGGAGGCCCTCCTGAGGACTGAACACTCAATCTGTCGA
GGAGTGCGTTGAAGTCTATAGGCTGGCCACTCTCCTCCATCCTCTGAATCACGCTCATCACGTGTTCTCCTCTGAAGCCCATGCTCACTAGCTT
CTCTATCAGTTCACCGTATTTTGATCTTAAAACAGGCGGTGGTGGTGTCCCTGTGTTGCCACCACCTCCCTGATGCTGCTGAGGTGCGTACCCA
CCACCTTGTGGACCTTGCATGTAATGTCCTTGCTGCTGTTGCTGCGGAGGAGGCTGAGTTGGTGGGTATTGCATCCGCCCACCTTCATACATGG
CACTGGCATACCCGGGAGGAGGAGGAGGTCCTGAGGGTAGATACCCATCGCCTGTCTGTGGGCTATAGGACATCTTTGTCTGCTGAGGAGGAGC
CTGTGGTGGTGGTGGTGCACCGTATCCGTAACCTTGCATCGATTGTTGAGGAGGTCCAGCGTATGGTGATTGCATCTGCATGCTGCTTGGCAAC
GGTTCTACTGGGGATTGGTTGCTTGGTGGTGAAGGCGAGTATG |
Primers for UN35322 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GATACCCATCGCCTGTCTGT |
59.957 |
20 |
ATCACCATACGCTGGACCTC |
59.957 |
20 |
125 |
510 |
634 |
| 2 |
GATACCCATCGCCTGTCTGT |
59.957 |
20 |
GCAACCAATCCCCAGTAGAA |
59.933 |
20 |
171 |
510 |
680 |
| 3 |
GGAGGAGGTCCTGAGGGTAG |
60.066 |
20 |
GCAACCAATCCCCAGTAGAA |
59.933 |
20 |
190 |
491 |
680 |
|
|
