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Simple sequence repeat (SSR)
>UN46998
GGGACTTAAAAAAAAAGTCCCACCGATACTCTTCCTCTTCTTCTCCTCCGCCACATAGCAACGAAATTAACAAGAGGAGGATGACCAGAAGTTG
TGAACGGATCAAACCTCAGTTGGGTCAGATCGAGGAAGAAACCCAACGACTCTCCACCATCCGTGATGTCAGATTTTCTCTCTCTCTCTCTCAC
TCTCTCTGTTTTGAATCTGACGGCATTTTGACAGAGGTGAACCCACGGCGGAGCTTCTCATCTTACGTTGCAGAGACGGGGAAGACAGAGGAAG
AGTACGACGGGCGGTGAGAAATAGGAGCTACATTGGTTTGTAATTTCTCTTCTTAGTTCTCTCTTAAGTTTCGGTTTGTGGTTTTTTAAGGTTT
GGGTTTCAATTTGTATCTGATCTATCTGTTTCTTTGTGCAATGGTTTCTTCTATCTGATTAATCTTGTCTCTGGTCTTCACTATCTGTGTAATG
GTTTTGATTTGTGGTTCTTTGTATCTGATCAATCTTATCTCTGGTTCTTTGTATCTGATTAATCTTGTCTCTGCTTCTTTGTGTAATGGTTCTG
ATTTGTAGTTTTTTTAGGCGATGCCTGATGTTTCTTAAATTTGGGTTGAGTACAGGAATAGATGGACAGGGCTTGAGGTTGGAAGAATAAGTTA
TTTGAGATCCTTATGTGTATGGGTTTGCTTGGTTTCTGCAACTCTTCCTCACAATCTTTAG |
Primers for UN46998 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ACGGATCAAACCTCAGTTGG |
59.966 |
20 |
CTCTGTCTTCCCCGTCTCTG |
59.978 |
20 |
181 |
98 |
278 |
| 2 |
TGTGAACGGATCAAACCTCA |
60.088 |
20 |
CTCTGTCTTCCCCGTCTCTG |
59.978 |
20 |
186 |
93 |
278 |
| 3 |
ACCTCAGTTGGGTCAGATCG |
60.112 |
20 |
CTCTGTCTTCCCCGTCTCTG |
59.978 |
20 |
172 |
107 |
278 |
|
|
