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Simple sequence repeat (SSR)

>UN50009
GACTCGGAATATATAGTGTTCTTGATTCTTTATTTGTTGTAATAGGAGGAAACAGCAGTAGCTTAACCAGATAATAGATGGTGGTTAGAGCTTC
TTTCCTCCCTTTTTCTTAATGGACAAACGAATAAAAAAACAGAGCAGAGCTATTATAGGGTAGGTAGACGGGAGACGTACATACTAGCTAGGGT
TCTACTGCTATTTCTTCTTCATCTTGATGTATCATGTTGACAAGCATATCGACTTGCTCTTTCAACATTGTGTTCTCTCTTTCAATCTCTGTTC
TCTTGCTTCTCTCTTGTAGTAGCTGAAGTTGCAGATGCTCTAAGCAACGTGAGTCCTCTTCTAGCTGCTCGTTTAACCCATCTATCTCTCGATC
CTTTCTGTTCATTTCTTGGACAGCGATTCTACGCATGCTCTCAACCACATCAACGTTCACCAGCTTCCTCTTCTTACCAATCAACCAGCCTTTA
GGGTAGTTTGCAGACTCGAAATCTGGAGGTGGCAAGAGGGAATCAGCATCTGTTGGTCGCTTTCCGTTCAAGTTTGTAATGGGTTCGAGTGGCT
CAAACAGAGCTTTTCTTTTCTCGGACAGAACAGGAGGAGTTGTGGTCATTGGTTCCCCAACTTTCTCGGTTTCCATCGCTGTTGTCTCATCCTT
CTCTGGTGTGACCTCCATAGCAGCTTCTAGTGCCTTGTCCTGGTTCACAGATGCTGATCTTTTTCTTGACTTCCACGCGATCTTGGTGGTAGAG
ACAGGGAAAAACCCTCCCACAAATCCATCACCAACTGTCTTCTTCTTCTTCTTGTTCTCTCCCTCAAATCCATTCCCTTTCTCCATTTTAATAC
GAGGGAGAGAGAGAGAGAGA
GGTTGAACGGTAAAGGTTTGGCGTTTCGGTTGCAACTCCC

Primers for UN50009 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 AAAAACCCTCCCACAAATCC 60.032 20 ACGCCAAACCTTTACCGTTC 61.254 20 132 759 890
2 GGAAAAACCCTCCCACAAAT 60.032 20 ACGCCAAACCTTTACCGTTC 61.254 20 134 757 890
3 GAGACAGGGAAAAACCCTCC 59.912 20 ACGCCAAACCTTTACCGTTC 61.254 20 141 750 890