 |
Simple sequence repeat (SSR)
>UN50022
AAACCAAATGTTCTGTTATTATTAAGAGAAATAAAGATTGTAACTTTAAGAATTACAAAAGTCAAGAGTAGACAAAGCCCTCCATAATCATGGG
AGACATAATCAGAGTTCCAGACCATTTATATATACCAAAGATATATTCTTAAAAGCCCAGAATCACAGAGATGCATTACATACAGATTCCTTTT
TTTCTTTCTTTTAAGTGGTTTTAGCCAAGGTTGCAAATCTTTCAAGTCCACCTCTAAGCAAGGTCTCTAAAAAGGGCCGGAGCGCGGATGCCAC
AGGGGTCAAAAGCGCAGGGACTTAATATGATACAGTTAGCTCAACGAGGCAAGATGAAGGACCTTTAGGGAAGAACCTAACACTGCCTTTATTA
GGAAGACCTTCAAGAGATCTCCAATGGATTTTCTGATTAGGAGTAGGCTGAAGATTGCGAGCAAGCCAAGAATACTTAATGTCTTGGCCAAAAG
CATTGTACTTGAGTGACCAACGTGATAGATCAGGCTTGTCCTTTAACACCTCAACGGATGAAATGAAAGGCATCCACTTAGGGAAAGATTCACG
ATCCAAGTAGAAGTTATACGCTACTGACACTGGTATATCTACTTCCATCTTCACTGTGCAGTCTTGCCACTCCATGGAAACAGAGAAGCGTTTG
TTGCGAGTGTTGAGTCTCGATCTTCGAGTGACGGTAGACGAAGGAGAGCGAGAGGATGAGGAGGAGGAGGAGGAGCCATTTAGGGGAAGCAACT
GAGGAAACGATTTGGGTTTACGATGAAAAGGTTGAGGAGCCAATCTGTGATTGGAGCAGATGAGATTGGATGTTTTACAGGTTGTCGACAAATG
ATTGATCGCGTTCATGTGAGTTGCTGCTGTTACAGACATTGGAATCTTTTTCTCTGTTTTTTTTTTTTTGCCTCTT |
Primers for UN50022 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGAAAGGCATCCACTTAGGG |
60.066 |
20 |
GCTCCTCAACCTTTTCATCG |
59.813 |
20 |
259 |
534 |
792 |
| 2 |
CACTGTGCAGTCTTGCCACT |
60.096 |
20 |
GCTCCTCAACCTTTTCATCG |
59.813 |
20 |
177 |
616 |
792 |
| 3 |
CACTGTGCAGTCTTGCCACT |
60.096 |
20 |
TCCTCAGTTGCTTCCCCTAA |
59.807 |
20 |
142 |
616 |
757 |
|
|
