 |
Simple sequence repeat (SSR)
>UN50159
AAAAACAAAAAACTCTTACCTTTTTGTTTTCTTCTAGATTTTTACCGACGAGAGAATAAGGAAGAGACAAGCCACGATCCTCCATAGCTGTACA
TAGATAGATCCCCACAAAGAAAAAGAAACCCTAATCAATCAATCAATCATGCCTTCCGGTGCTAAGAAAAGGAAAGCCGCCAAGAAAAAGCAGC
AACAAGAAGCCTCTTCTACTCTCAAATCAACCAACCATGGATCTACCGAGGAAGAAGCTGTCAGAATCGTAAAGGAAACAGGGTTGGATACTAA
TGAGCAGCGTGTAGAGATCACTGATTCAAGCCATGATCATGACAAGAGCTCTAGCAGCAACAGCAGCAGTAGCGGTAGTGGTAGTGGTAGCAGC
AGCTCTGATGATGATGAATCCCATGAAGTCAAGGAGGAGACTGAAGTGATCATCACTGTGCCAAGTCAACCTGTTCCAGTTGCTGGAGACGCTC
CCTTTATTGGTTCTACTGCTAATGCGATTGTAGAGAATGCCGGTTTGATGGATTCAACTACACCTAGTGATCCAGAGACTGAGAACATTGTTGT
GACCTCGTCTGCCGCTGAAGCTTCTCTTGCATCTGATGAGAGTAAGCAGGCGTCTTCAAGCACGACTGGCTCTGATAAAAAAGAACTAAAAAAA
TGTGTTCCTGAAGGGAGCAAAGAGTCTCAAGTTGTCATTTCTCATGTGGAAGAGGATCCCGTGCGACCAACACATGGAGTTGCGCAAAGAACCT
CATGGTTAAGTTGTTGTGGCTTGTTTGATGTGATGACAGGATCCAGTAGATAAGCTCAGAAACTTGAGTCGTCGCCTATGAAGTCAGAAGCTGC
AATATGCTAAATGTAATGAATGTCTCTCTCTCTCTCTTTCACTTAAGAACACAAAACATACCTTTTCTCATATGAA |
Primers for UN50159 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GTGTTCCTGAAGGGAGCAAA |
60.232 |
20 |
TGAGAAAAGGTATGTTTTGTGTTCTT |
59.553 |
26 |
257 |
660 |
916 |
| 2 |
AGTTGCGCAAAGAACCTCAT |
59.882 |
20 |
TGAGAAAAGGTATGTTTTGTGTTC |
57.436 |
24 |
181 |
736 |
916 |
| 3 |
AGTTGCGCAAAGAACCTCAT |
59.882 |
20 |
TGAGAAAAGGTATGTTTTGTGTTCT |
58.374 |
25 |
181 |
736 |
916 |
|
|
