 |
Simple sequence repeat (SSR)
>UN50226
GGAGACTCATCATCCTCGGCTTCGACTCGATACTCATTCTCTCCGATTTAGGGATTCCATACTCGCCATGTATCGCACGGCTCGTTATGCAAGC
GCTAGTGCTGTTGCTACGACCTCTTCTTCTTCTTCTTCTTCTCCAGGTTTCTTAGGCTGGTTGACTGGTGGATCCTCTGGCTCCCTTGCTTCAC
TGGACATGCCACTACAGGGAGTATCTCTTCCTCCGCCGCTTCCTGACCGCGTCGAGCCAAGCAAACTCAAGATCACTACTCTTCCTAATGGCCT
CAAAATCGCCTCTGAGATGTCTCCCAATCCGGCAGCTTCTATTGGTTTGTATGTTGATTGTGGTTCGATCTATGAGGCTCCTTATTTCCATGGA
GCAACGCATTTGCTTGAAAGGATGGCTTTCAAGAGCACCACGAACAGAAGCCATCTACGTCTCGTCAGGGAAATAGAAGCGATTGGAGGGAACA
CCTCTGCGTCTGCGTCTCGGGAGCAGATGAGTTACACTATTGATGCTCTTAAAACCTATGTCCCTGAAATGGTTGAGGTTCTTATCGACAGTGT
TAGGAACCCTGCTTTCTTGGACTGGGAAGTCAATGAAGAGCTACGTAAAATGAAGGTGGAGATAGCGGAACTTGCGAAGAACCCTATGGGGTTT
CTCATTGAGGCTGTTCATTCTGTTGGTTATTCTGGTGCATTGGCTAATCCTCTGTACGCACCTGAGTCTGCCTTGCACAGATTGAATGGGGAAC
TCTTGGAAGAGTTTATGACTGAGAATTTCACTGCTGCACGTATGGTGTTGGCGGCAAGTGGAGTTG |
Primers for UN50226 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGATTCCATACTCGCCATGT |
59.78 |
20 |
CGATTTTGAGGCCATTAGGA |
60.031 |
20 |
239 |
52 |
290 |
| 2 |
GGATTCCATACTCGCCATGT |
59.78 |
20 |
AGGATCCACCAGTCAACCAG |
59.962 |
20 |
119 |
52 |
170 |
| 3 |
GACTCATCATCCTCGGCTTC |
59.768 |
20 |
AGGATCCACCAGTCAACCAG |
59.962 |
20 |
167 |
4 |
170 |
|
|
