 |
Simple sequence repeat (SSR)
>UN50234
GGACGGTTCTTAATTTTCTCGACGAGATCTCTCTCTCTCTCTCTCCACCTTCCACCACCGTGCGGAGAGGGATGTCGAAAGCTCGTGTTTACAC
CGACGTCAACGTGATCCGACCCAAGGAGTATTGGGATTACGAGTCTCTCAACGTTCAGTGGGGGTTCGTAAAAATAATCCCCTCTTTCTGAAAA
CCCTAATCGATTGCGTTTCATGTTGATTTTTTGACATCTTTCCTCGGTAGATTTATTTTGATTTTTTTCGCTATAGCTCTCTGTTCATTCATGA
TTGTTGATTCCAAGTTTGAGTTTTGATCTGTCTGAGGAGAGTTAGTTTTGTATATGTTGATGATGTTTTGGTTAGAAAGTTCTTCTTTTGTGTG
TTGTTAATAATCTCAGGGAGCAAGATGATTACGAGGTTGTCAGGAAGGTGGGGAGAGGCAAATACAGTGAGGTGTTTGAGGGGATTAACGTGAA
CAACAACGAGAAGTGCGTTATCAAGATTCTCAAGCCTGTCAAGAAGAAGAAGATTAGAAGAGAGATTAAGATACTTCAGAATCTCTGTGGAGGG
CCAAATATTGTCAAGCTGTTTGATGTTGTGAGAGACCAACACTCAAAAACACCAAGCTTGATATTTGAGTATGTTAACAGCACTGACTTCAAGG
TTCTGTATCCCACTTTGACTGATTATGACATCCGTTACTACATCTATGAGCTGTTGAAGGCGTTGGACTACTGCCACTCGCAAGGTATAATGCA
CAGAGATGTCAAGCCACACAATCTCATGATTGACCATGAGCTCCGCAAACTTCGCTTAATTGATTGGGGGTCTCGTTGAGTTTTATCATCCTGG
AAAGAGTATAACGTCCGTGTGGCCT |
Primers for UN50234 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CGGTTCTTAATTTTCTCGACG |
58.886 |
21 |
ATCACGTTGACGTCGGTGTA |
60.032 |
20 |
106 |
4 |
109 |
| 2 |
CGGTTCTTAATTTTCTCGACG |
58.886 |
21 |
TACTCCTTGGGTCGGATCAC |
59.927 |
20 |
121 |
4 |
124 |
| 3 |
CGGTTCTTAATTTTCTCGACG |
58.886 |
21 |
TTTACGAACCCCCACTGAAC |
59.83 |
20 |
163 |
4 |
166 |
|
|
