 |
Simple sequence repeat (SSR)
>UN50322
GGGTCAAACCAGTTGTTCAAAACTTTTGAAGCTGAGGACAATAGAAGATCCATTAAGTCCATTCACAAGACAAAGCAATACACAAAATAAGAGA
TCATACTGTCTCATTCTCTCCCATTCACAATGGATCCAATCCAATAGGAATGAATAGAAGAAACTGAAAGATTGTTATTACAATCATGCGAATA
CGTACGTTACATAAAGAGACAAGGTGTTTCTTCTTCAGAAACGTTATTGAGATGAGACGATCTTGATCATGGCTTGTGCATTGAACCCTGTTAG
ATTCTTCTCACAAAACCTCTTCCAAGCTTCTTGTTTGTTTCCTTCTTCACCTTCTTCTTCTTCTTCTTCATTCCAAGTGAATTTCTCGTAAACA
GAGTTCACCATACTCCTCGCAGCCTCCCTTGCCTCTGGTAACTTATCATTCAACAAATCCGCAGCCATTTGCACAATCATAACCATCCCGAACT
CCTCCATTTCATTTACCTCCATTTTAGAAACGCAGTTCGAAGTAGACACAGCAGCTTTGGCCCTAACACGAGGGTTACTGTGTCGGACATAAGC
TTTGAGCTTACGCAGAAGAGGCAAAGAATCCACCGAGTTAACCATCGTGTTCAGCGTTTTGTCTGCTTCTTCACAAACAAACTTCTCGTCTTGT
GAAGCTTTCATTAGCAGCTGTAATAACATATCGTCCATAGTTTT |
Primers for UN50322 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ATCATGGCTTGTGCATTGAA |
60.08 |
20 |
GGAGTTCGGGATGGTTATGA |
59.75 |
20 |
219 |
254 |
472 |
| 2 |
TTGATCATGGCTTGTGCATT |
60.08 |
20 |
GGAGTTCGGGATGGTTATGA |
59.75 |
20 |
222 |
251 |
472 |
| 3 |
ATCATGGCTTGTGCATTGAA |
60.08 |
20 |
GAAATGGAGGAGTTCGGGAT |
60.272 |
20 |
227 |
254 |
480 |
|
|
