Simple sequence repeat (SSR)
>UN50454
GAGGCAGCACCAAGAGGGTCACGTCCAGAACGCCAACCTCGGGTGTATGAACCTGCGTTCAGAGTCTATGTGGGAAACCTACCGTGGGATGTGG
ACAACGGTCGTCTAGAACAAGTTTTCAGTGAGCATGGTAAAGTTGTGGAAGCTCGTGTGGTTTATGACCGTGAGACAGGTCGTTCACGTGGGTT
CGGTTTTGTCACAATGTCTAACGAGACTGAACTCAACGACGCTATTGCTGCTCTCGATGGACAGAACATGGAGGGTAGAGCAATCAGAGTGAAC
GTAGCGGAGGAGCGTCCGAGGCGCGGGTTTTAAAAGGATTGAGATTATCTCTTCTGCCAGTTTCCTCTCTCTCTCTCTTTTATCGCTTTGCATT
TTGTTTAGACGAGATTCTTACGCTCTGATGATTTTTTTTGTTGTGTCCGAACACCTTTGATGATTTTTTGT |
Primers for UN50454 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GAGTGAACGTAGCGGAGGAG |
60.012 |
20 |
GGTGTTCGGACACAACAAAA |
59.44 |
20 |
157 |
275 |
431 |
| 2 |
AGAGTGAACGTAGCGGAGGA |
60.012 |
20 |
GGTGTTCGGACACAACAAAA |
59.44 |
20 |
158 |
274 |
431 |
| 3 |
ACGCTATTGCTGCTCTCGAT |
60.147 |
20 |
GGTGTTCGGACACAACAAAA |
59.44 |
20 |
205 |
227 |
431 |
|
|
