Simple sequence repeat (SSR)
>UN50577
GGAGAAACAGAGAAGGAGAATCAGGAAGGAAGGAAGGAAGAAGAAGATGTCGTTGGTGTGGCTAGAAGCGGCTCTGCCTCTGGGAATCATCGGA
GGAATGCTCTGTATTATGGGAAACTCTCAGTACTACATCCACAAAGCTTATCATGGCCGTCCGAAGCACATCGGGCACGATGAATGGGATGTTG
CTATGGAGAGACGCGACAAGAAAGTCATCGAGAAAGCCTCAGCTCCTTCGTCCTGATCCAATCAGGTGAACAACTTGTGGTCACAAATAAAGTT
TATTCCAGAAGAAGAAGAAGAAGAAGCTGGAGAGTCTAGTAGTGTTTTTTCCCGTTTGATTTTTCTTGGAGACAAGGTCGGATAAAGCTTTCAA
GCTTAAAAACCTTTTTTTTTTCTGCTTCCAAAATTCAGAGTTCAAACCTTAATGACATTTGGTTTATAACTTGTCTCCATTTATTTTCATGACA
TTTGGTATACAATACACTTCTTGCTGTGTGAATGAATAATAATATTGAA |
Primers for UN50577 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TCCTTCGTCCTGATCCAATC |
60.011 |
20 |
TCCAAGAAAAATCAAACGGG |
59.91 |
20 |
121 |
232 |
352 |
| 2 |
TCCTTCGTCCTGATCCAATC |
60.011 |
20 |
CCAAGAAAAATCAAACGGGA |
59.91 |
20 |
120 |
232 |
351 |
| 3 |
GTCATCGAGAAAGCCTCAGC |
60.104 |
20 |
CCAAGAAAAATCAAACGGGA |
59.91 |
20 |
140 |
212 |
351 |
|
|
