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Simple sequence repeat (SSR)
>UN50614
GGGGTCGTCTTCTTGCAGAGAAAAAAAAAATTAAAAGAGCCTCGAAAAATTCACAGACTTGTCTCCCTCTCTCTTTCAGATTTTAGTAGCTTCT
GAAAAATTTCTCTAAAAGAAAAAAATGAGCAAGTTCTGGACTTTCCTCACTGCTCTTCACTCAGGCGCTGGCCCACTGGTTATGTTGGTGTATC
CATTGTACGCATCGGTTGTGGCAATGGAGAGCACAACGAAAGTAGACGACGAGCAGTGGCTTGCGTACTGGATCATTTACTCCTTCCTCACCCT
CACGGAGCTGATTCTGCAATCCCTTCTCGAGTGGATCCCGATTTGGTATTCTGTGAAACTGGTGTTCGTGGCTTGGTTGGTCCTGCCTCAGTTC
CATGGCGCAGCTTTCATCTACAACCGCGTGGTGAGAGAACAGTTCAAGAAACACGGCGTCTTCGGCTCCTCCTCCTCCTCTCACACCAAACACT
CCAAGCACTCCAAACCCAACATCATCCACTCCATTTTCCACCGGGAGGGACAAGAGGCTCACTCTCACTGACGCCAAAGCGGACGAGAAGCTCG
AATAGCATTGTAATGGGAGTTGGTGGTGGCCCCTTCTTGTCTTCATGTCTAATCTTACTTTTGCTTTAGTTTTTCATTTAATAATTTGAGAAAT
ATGATAATGACTGAGGAGGTGTGTGATCGTTTTAAATTAGAAATGGCTTCTTGTTGTTGCATTGCTTTGATTCCACGACGACGTATTTTCCATT
TGGAACACAAGTGTTAACGCTATTGTCCC |
Primers for UN50614 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGCTTGCGTACTGGATCATT |
60.103 |
20 |
GATGTTGGGTTTGGAGTGCT |
59.973 |
20 |
248 |
246 |
493 |
| 2 |
GAAACTGGTGTTCGTGGCTT |
60.156 |
20 |
GATGTTGGGTTTGGAGTGCT |
59.973 |
20 |
157 |
337 |
493 |
| 3 |
GGCTTGCGTACTGGATCATT |
60.103 |
20 |
TGGAGTGCTTGGAGTGTTTG |
59.873 |
20 |
237 |
246 |
482 |
|
|
