 |
Simple sequence repeat (SSR)
>UN50822
GGGAGAGAGAGGATGATGACTATGATGATGTCTCTCCCCAAAATGAAACTATCTTCTCTCCAACCTCATCTCCATTTCAGCAGTAGCAGATTAC
TAGCAGCACCAGCTAAGGCGTCTCCGAGCTCAACCATAAGCACCAGAACACCGTGCCGCCGTGTTCAGTGCCAAGCATCCAGAAGAAGAAGAAG
ATCAACGGTGGAAAGATCCACCAGAACCAGGCTTGAAAAGACACAACCTTTGTCTCTGGAGAAGAAGCAACTGAAACAAGCTCTGGTGACTGGT
GTCTCCGTAGGGTTGATGATGGCTTTGGCATTAGGAATAGATGGAGAAAAGGCGATGGCTTTGGGACCGGAAGGTCCTTTGATGGAAGAGTTCT
GGGACAACGTGAGAAGGTACGGTCTTTACGCACTCACCGTCAGCACGGGAGCTCTCTCCGCCGTGTTCGAGCCCATCTTCGAGCTCCTCAAGAA
CCCAATCTCCGCCGTTCTCATTGTGATCATCTTAGGTGGAAGCTTCTACATCGTCTCCCAAGTCGTCTCAGCTATGGTTGGTGTCAACGAGTTC
GCTTATGATTATGGATACTGAAGAGAGTTTGTTTGTTTGTTCTCTTTCGTTAAATAAATACTCTGTTTCTTACATGTTATGGTCATCGATACTC
TGTTTTATGCATTTAGTCTTATAATCTTTTGTTACATTTTATTTGACATAAA |
Primers for UN50822 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CTCCGAGCTCAACCATAAGC |
59.978 |
20 |
TAATGCCAAAGCCATCATCA |
60.035 |
20 |
200 |
116 |
315 |
| 2 |
CTCCGAGCTCAACCATAAGC |
59.978 |
20 |
TTCCTAATGCCAAAGCCATC |
60.038 |
20 |
204 |
116 |
319 |
| 3 |
CTCCGAGCTCAACCATAAGC |
59.978 |
20 |
AAGCCATCATCAACCCTACG |
59.955 |
20 |
192 |
116 |
307 |
|
|
