 |
Simple sequence repeat (SSR)
>UN50827
CTTCTCAAACTCTAGTTTTCGTGACTATAGCACTTGTCCTAGCTTTTGCAGTTCCTCTCAAAGCCCAAGACAGGCAACAAGATTACCTCGATGT
GCACAACCGTGCTCGTGCCGCTGTTGGTGTGGGCCCGATACGATGGCATGCGGATGTGGCGGAATACGCCCGACAATATGCTCTGCAAAGGCGA
GGGGACTGTCGTCTCACTCACTCAGGCGGGCGTTACGGCGAAAACTTGGCAAGAAGCACTGGTGATCTGTCTGGAGCTGACGCAGTGAGGTTGT
GGGTCAACGAGAAGAACGACTACTTCTACAGTTCGAACACTTGTCGTTCCGGAAAAGCATGTGGTCACTATACTCAAGTTGTGTGGAGAAACTC
GGTGTGGGTTGGATGTGCCAAAGTCAGGTGTAACAGTGGTGGAACTTTCATCATTTGCAGCTATGCTCCTCCTGGTAATGTTAGGGGCCGTTGG
CCTTACTAATTATGAATATATATGTACGCAGATGAATAAAACATGTCCATAGCGGACGTACGTGTGTGTGTGTTTTTATCTTTCAAATCATGAT
TACCATCTACCAGTATATGGTGATTCCTTAACGTTCATGTGTATGGTTGTATAATATTTTGTGATGGAATAATACATTGATAAATAGTTGTCTG
TACGTACAATTACAAATAAATGCATTGCATGTGGACATGCAAG |
Primers for UN50827 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TTTGCAGCTATGCTCCTCCT |
60.118 |
20 |
CATGAACGTTAAGGAATCACCA |
59.861 |
22 |
174 |
430 |
603 |
| 2 |
TTGTGTGGAGAAACTCGGTG |
59.72 |
20 |
CATGAACGTTAAGGAATCACCA |
59.861 |
22 |
243 |
361 |
603 |
| 3 |
GAGAAACTCGGTGTGGGTTG |
60.545 |
20 |
CATGAACGTTAAGGAATCACCA |
59.861 |
22 |
236 |
368 |
603 |
|
|
