 |
Simple sequence repeat (SSR)
>UN51414
GGGTCTCAGCTAAAGCTGAAAACAATTTATCAGTAAAACAGAAGTAATCATGTCTTTTTCAAGGATTAAGAGAGCTTTATCACAACCAAATTTG
TCTTCTTCTTCTTCTTAACACTTCACTAGAGAGTGGCAGAGAGAAGAGAGAAATTACAAAATGGAGCAGGCCTTTTGTACTTTTCCCTTCTTTT
TCTTCTGCTTTGGAGGTTGAAGTACCACTCTAATCGCTGCATCAAACACCCCCTTCACGTTCTCCTGTGATTTTGAACTGCACTCGATGTACGA
AGGAGCTCCAATTAGCTTCATTAGCTCCTCGCCCTGAGCAGTGGTAATAGGTACAGCACCAGGGTGGTCAACAAAGAACTGTTTGTCATCCCTA
AGATCTAGTTTGGTTCCAACAAGAACAATTGGGACACCAGGGGCATAGTGAGTCAGCTCAGGGATCCACTTCTTGGAGACATTCTCATAACTAG
CCTTACTGATGAGGGAGAAGGCTAAGATGAAAACGTCAGCACCACGGTAACTCAATGGTCTTAACCTGTTATAATCCTCCTGCCCAGCGGTATC
CCACAAGCCAAGGTTGACAGTGGCTCCGTTAACAACAACATTAGCGCTAAAGTTATCGAAAACAGTCGGAACATAATCCGTAGGGAAAGTGTTG
CTAGTGTAAGAAATGAGCAAGCAGGTTTTACCCACTGCTCCGTCACCGACGGTAACACACTTTATGAACCTCGAAGCGCTCATTTTTCCTCCGA
AGTATCAAAACCTCAGATCTTCTACACCTGAGGAACACAACAACAACAACAACAAGATCGTCCTCAATCGAAACAAAGTGTCTCCCTGCTCCCC
ATTTCAATCATTCGGCGCAAAAAAGAACCCTAGGAAAGATGATGTTTAGAATAGGAAACTCTAAGCGAAGTTGGAAATGTGAGAGAATGAGAGA
ACAAGTGTGTATGATCAAAGAAG |
Primers for UN51414 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGTCTTTTTCAAGGATTAAGAGAGC |
59.491 |
25 |
TGATGCAGCGATTAGAGTGG |
59.972 |
20 |
182 |
51 |
232 |
| 2 |
TGTCTTTTTCAAGGATTAAGAGAGC |
59.491 |
25 |
AGTACAAAAGGCCTGCTCCA |
59.875 |
20 |
125 |
51 |
175 |
| 3 |
AAGAGAGCTTTATCACAACCAAA |
57.285 |
23 |
TGATGCAGCGATTAGAGTGG |
59.972 |
20 |
165 |
68 |
232 |
|
|
