 |
Simple sequence repeat (SSR)
>UN52223
AGGGACAACAAACACCTTACACCCTTTGTGCCCTCTCGAAATCGAAATTATCAATCGCTACTCCGGTTCGGGGGAAGACGACGAGACGACGACG
ACGACGAAAAGAGATTTTTGGTAAAGAAATGGATGTGCCGGAGGAGACGAGGCTTCGCCACAAGCGTGATTTCATCCAGTTCCTCGAAAACATC
TACATGGATGAAATCAAGGCGCTGGTACAGAACAAGCGTCACCGTCTCATCGTCAATGTATCTGACATTCATACTCACTTCCGCGAATCTGAAT
CTTCCTCTAGGATATTGAAGAATCCAATTGAGTATATGCAGTCGTTTTGTGACGCAGCCACTGAAGCTACACGCAACATTGACCCCAAATACTT
GAAAGAAGGAGAGCAAGTCCTTGTTGGATTCGAAGGTCATTTTCTCTCGACGTGTCACACCTAGGGAGCTCCTCTCTGAGTTTATTGGATCTTT
GGTCTGTGTTGAAGGCATTGTCACCAAATGCTCTCTTGTGAGGCCTAAGGTTGTTAAAAGCGTACACTTTTGTCCTTCTACTGGTTAATTTACC
AATCGAGAGTATCGAGACATAACGTCTCATGCCGGTTTACCTACTGGCTCTGTCTATCCCACAAGGGATGATAATGGAAACTTATTGGTGACTG
AGTATGGGCTCTGCAAATACAAAGATCACCAGACCTTGTCAATTCAAGAAGTTCCTGAAAATGCTGCTCCTGGTCAGCTTCCACGAAGCGTTGA
TGTCATAGC |
Primers for UN52223 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TACACCCTTTGTGCCCTCTC |
60.111 |
20 |
CTGTACCAGCGCCTTGATTT |
60.27 |
20 |
201 |
18 |
218 |
| 2 |
TACACCCTTTGTGCCCTCTC |
60.111 |
20 |
ACATTGACGATGAGACGGTG |
59.551 |
20 |
230 |
18 |
247 |
| 3 |
TACACCCTTTGTGCCCTCTC |
60.111 |
20 |
CTTGTTCTGTACCAGCGCCT |
60.453 |
20 |
207 |
18 |
224 |
|
|
