Simple sequence repeat (SSR)
>UN52529
GGAAACGATCGATCAATCTGAATCTTCTCATTTTCCCCCTTTCATAGCTAGCTCTAGGGTATTCGAATCGAGAAGAGATGGGACAGACCCAGTA
CTCTGACAAATACTTCGACGATACTTTCGAGTACAGGCACGTCGTGCTTCCTCCTGAAGTCGCTAAGCTTCTTCCCAAGAATCGTCTTCTCTCC
GAAAATGAGTGGCGTGCGATTGGTGTGCAGCAGAGCCGTGGATGGGTTCATTACGCGATCCATCGTCCAGAGCCACACATCATGCTGTTCAGGA
GGACTCTCAATTACCAGCAGCAGCAGCAGGAGAACCAAGCTCAAAACGTGCTCGCTAAGTGAATCTGAGTTGTGCTGCGCTACAAACATTGGTT
CTTGTTAGCTATTATGTTTCTTGTAAACATGGGTTTGTGAACCACAACCTCTAATAACTTTGTGTTCATGTTATCTATCCTTGGTTGGAGAAAA
ACATGCGAGTGAATGAGTGATAATCTGACTACTTTGATGTTATGATGCATTGTCTTTGTTCATCGTGGATTATTATATCGTGTTTCGTCTTAAC
|
Primers for UN52529 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TCGCTAAGCTTCTTCCCAAG |
59.724 |
20 |
TTTGTAGCGCAGCACAACTC |
60.203 |
20 |
215 |
154 |
368 |
| 2 |
GCTTCCTCCTGAAGTCGCTA |
59.717 |
20 |
TTTGTAGCGCAGCACAACTC |
60.203 |
20 |
229 |
140 |
368 |
| 3 |
TCGCTAAGCTTCTTCCCAAG |
59.724 |
20 |
TAGCGCAGCACAACTCAGAT |
59.77 |
20 |
211 |
154 |
364 |
|
|
