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Simple sequence repeat (SSR)
>UN53189
GAGACGAAAACGTGTTTTGTTTTTTTAATGAAACACTTGTTCTTCTGTTCCTCATCTTTATAAACGAAAATCTACAAAGGAAGAGAGAAAAAGA
GAGGGCAGATAGATATAGACGCTTCTTCTTTTAAAAACAGAACATGTGCTTCCTCTTCTTCTTCTTCTGGGACTGAGCAAACTCTTGACGATCA
ATGTAAGCGAGTAATTCTTCTTGCCTCATCAAAGCTTCATACAGAGCCTTTTTCGTGGCTATGAGCTCGGCTTCAAGTGCGTCTACACGGCAAG
CAGCTGCGTTAAGCAGTTCTTCTTTCTCGGAAGGCATCTCATTCGGCTTTGCCTGCAGCGCTCCTATTTTCTCCTCAAGTTCCGTTAATTTCTT
CAGCACTGAATTGAGGAGCTCAGCTTCCTCCTCTCTAGCGGCAGTGTTCCCTTCTATTCCCGGTGGAGGAGGAGGAGGAAGCTGCTTGGTCACA
ACACGGTCTGATAGTGTGCGGAAGAATGTGAGAATCGCCATCACAATCGCCATGATAAATACAAAGACACGAGCACTGAAGCTCTCATGCTCCT
TTGGTTCATTTGGAGTAACAAGAACTCCTTTGGATTGAACCCTGTTGATGGTG |
Primers for UN53189 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCCTTTTTCGTGGCTATGAG |
59.845 |
20 |
ATCAGACCGTGTTGTGACCA |
60.005 |
20 |
249 |
234 |
482 |
| 2 |
GAGCCTTTTTCGTGGCTATG |
59.845 |
20 |
ATCAGACCGTGTTGTGACCA |
60.005 |
20 |
251 |
232 |
482 |
| 3 |
TCCTCTCTAGCGGCAGTGTT |
60.156 |
20 |
TCCAAATGAACCAAAGGAGC |
60.051 |
20 |
174 |
405 |
578 |
|
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