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Simple sequence repeat (SSR)
>UN53313
CATCGCCTAAAAAAAAGAGGATCTTCATTACTTCAACATAGTTACACATGAAACCGAATCAATCAAGCACACCGGGAAACTCAGTTTAGATTTT
CAGACATCATCGAGTCTTCTCTTGGACTGAAGAGCTAAACGAGCGTCTCTCTCTTTCTCAAACTGTCTGTAGTCAGCTCTCTCCAAGAAAGAAA
CTTTGTCAAGATACTTGTCAGAGCTCTTCTTGTACTTATCCAGCTCGTCTTCAACACCTTTGTTCTCCTCCTTGTACTCTCCCCAGTCTTTCTT
CGTCTTGTCCAAAACGCTCAGCTTCTGTTTCTTTTTAATCTGCTCAAGAACCGCATCAACCCCTGAAGTGGCCACAGAGGAAGAGGAGGAGGAG
GAGGCTTCCTTTGAACCCGCTTCCACTAATCTCTTCACTTCGATTTCTTGGCCAGCAAAGTCTTTGACCTCCGTTATCCCAACTTTGCCACGGC
TAGAAGCAGCAGCAGCGGTTGCGGCTGCGTTTCTAACAGCAGCTAGAGCGGCTGCAGCAATGCGCTTAGCCTCCTCCTCGCTAGTAACCGAAAC
GCTATCTTCGTTTTGCACAGCGTTGTGATTCTTCTTCTTCTTCTTCGCGTCATCAATACCAAGATAACTTTTCCAGTTGCTATTAGGTGACTTT
TTAGCGGTGGGAGGTGAAGAGGTTGAGGTCTTTGAGACGAAATTGAATCGCTTGTTTGGTACTCCTTCGTTCATTTGCTTCCAGATCTCTGAAA
CTTTCTTGGAAACGCTGCTCGAGCCAGTTTTC |
Primers for UN53313 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AATCTGCTCAAGAACCGCAT |
59.843 |
20 |
CGCTGTGCAAAACGAAGATA |
60.014 |
20 |
268 |
319 |
586 |
| 2 |
AATCTGCTCAAGAACCGCAT |
59.843 |
20 |
TGTGCAAAACGAAGATAGCG |
60.014 |
20 |
265 |
319 |
583 |
| 3 |
AATCTGCTCAAGAACCGCAT |
59.843 |
20 |
TCGGTTACTAGCGAGGAGGA |
59.971 |
20 |
243 |
319 |
561 |
|
|
