 |
Simple sequence repeat (SSR)
>UN53456
CGCAGAGACATTTAGCATTATAAATAATCAATCAAATGTTTGAATATGTTTTCACAGACACAAACACAAATACATCAACGGTCAAGAATCTGAC
CAGAAAAATAAAAGATTTATAAAGATTTCAGAAGGAAACAAAACACAAAAGCACAGCAAGGTTGATCTGTTTGAATCTAACAGCTAAATCAAAA
CTCTCGGCCAGGAGGTGTTTTGTTGTTGTTAGCTCCTCCTCCTCCTCCTCCTCTATGAGTCTCCTCGCTGCTCGAGGAAGATGGGTGAAGCCCA
TACTCGCTCGCGTTCCCATACTCGCTCGTGTTCCCATAATCCTGGCTATCCAACGCCACTTTCCTGAACTTCTTCATATCCGCACTGTAACTCC
CCGAGTCATAGTCAGAGCTTGAACCCCCCCCGAAGAGTGCGCTCTGTCCAGGCTTGGCTCCTTCGTTTAGATCATCTAGCGATGCATCTCCCTC
TAGAGCTCGAACAATTTGGCTCATCTTAGGCCTGCGTCTAGCTGAGTGTCTGATGGCTGCAACAGCACAAGCCACCATTCTTGCCATCTCATGG
CTCGTATTGGCCCTCGAGACGTGGATCAACTAGTTCACTGTAATCTCCGTCTTGAGCTGCGTTTAAGCACAAGGGTCTTGCCCAATCAGCCAAA
CTGTCTTCCATTTCACCAGTGAGATCAACAGGTCGACGTCCGGTTATGAGCTCAAGAAGCATCACTCCAAAGGAGAAAACATCTGACCTGTCTG
TCAGTTTACCGCTTGATGCATACTCTGGAGCCAAGTACCCGAAAGTTCCCATGATACGAGTGGACACATGTGTAACGTTGTCCTGAGATAGCTT
TGCTA |
Primers for UN53456 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCCAGGAGGTGTTTTGTTGT |
60.012 |
20 |
GGGGAGTTACAGTGCGGATA |
59.955 |
20 |
184 |
195 |
378 |
| 2 |
CAAAAGCACAGCAAGGTTGA |
60.027 |
20 |
GGGGAGTTACAGTGCGGATA |
59.955 |
20 |
239 |
140 |
378 |
| 3 |
GCCAGGAGGTGTTTTGTTGT |
60.012 |
20 |
GCGTTGGATAGCCAGGATTA |
60.06 |
20 |
144 |
195 |
338 |
|
|
