 |
Simple sequence repeat (SSR)
>UN53854
GGGAGATTCGCTGTCTTAAATTTCACTAACCTCTCATCTCTCTCTCTCTCACTTGTACGACTTCTCTTTGCAAAAATGGCGAAACTAGCGATGC
TGTTGGCTCTCTGTATCTTACCGGCGATAGCCATGGCGGCGAAGGTGCCACGTATCGGTAAGAACACGTTGGTGGTTCAAGGTATCACTTACTG
CGACACTTGCAAGTTCGGCTTCGAGACTGCTGAATCCTCCTACCCCGTCCCCGGTGCGACGGTGAAGCTTTCGTGCAGAGACAGGAAGGCAATG
AAAGAGTTATACACAGACGTAGCTGTATCAAACAGGAAGGGAAAGTACATGTTCGTTGTCCACGAGGGCCATAAAGACGAGATGTGCGATGTTA
TGCTCGTCAAAAGCTCAGACAAGGGTTGTTCTAAAATCTCGAGGGGGCGTGAACAGTCGCGTGTGATATTGAACCATTACAATGGCATTGCGTC
GCAGATCAGGCACGCTAACAACATGGGGTTTGAGAAAGATGTTTCTGATGTGTTTTGCTCTGAGTTGATTAAGAAGTATCAGGTTGATGAAGAT
GAGGTTTAGAGAGACCCATCTTTAATCAGTGTCATGATGGTCTGTTGTTTTCTATTTGGTTATGGGAGAGATATATTTGTATTTTCTTGATATA
ATATTATTTGTTCTTAGATGATGATGAGGATGACTCCGATGGGAACTCTTGTTTGTTTAAGTTTGTTCTATTGGTATATGTTCACTTCACTGGT
GGAATGAG |
Primers for UN53854 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGGAGATTCGCTGTCTTAAA |
57.032 |
20 |
GATTCAGCAGTCTCGAAGCC |
60.104 |
20 |
224 |
1 |
224 |
| 2 |
GGGAGATTCGCTGTCTTAAA |
57.032 |
20 |
GGATTCAGCAGTCTCGAAGC |
60.104 |
20 |
225 |
1 |
225 |
| 3 |
GGGAGATTCGCTGTCTTAAA |
57.032 |
20 |
ACCTTGAACCACCAACGTGT |
60.321 |
20 |
177 |
1 |
177 |
|
|
