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Simple sequence repeat (SSR)

>UN54053
GGGACTCACTCAAATCCACAATCTCCAAAAACATCCGAGATCTCCCTTCTCCCTTTGTGTGCGCTCCGTTCAGTTAGACATGGCCACCACCGCA
TCTCCAGCTCCGACGAAGAAATCGAGAAACAGCAGAAAGGCGTTGAAGCAGAAGAACGAGATCGTGACGGCGGACACTCCGTCTCCGCCGTCGC
CTGTCTCCGTCGCCAAGGGGAAGGGGAAGAAGTCGTTCGAGAAAGATCTGCTGGAGATGCAGGCGATGCTGGAGAAGATGACGATCGAGAAGGA
TAAGACGGAGGAGTTGCTTAAGGAGAAGGAGGAGATTCTGAGGAAGAAGGAGGAGGAGCTCGTGACGAGAGACGCTGAGCAGGAGAGGCTCAAG
ACGGAGCTGAAGAAGCTTCAGAAGATGAAGGAGTTCAAACCTAACATGACGTTTGCTTGTGGCCAGTCTCCCACACAAGGTGAGGAAGAAAAGG
TGAACAAGAAGAAGAAGAAGAAGGATTGTCCCGAGAGGAAGCGACCATCGTCGTCATACATTCTGTGGTGTAAGGAGCAGAGGGATGAAATCAA
GAAGGAGAATCCTGAAGCTGACTTTAAGGAGACTTCGAACATTCTTGGCGCTAAATGGAAGTCTCTTAGCGCGGAAGAGAAGAAGCCTTACGAG
GAGAGGTACAAGGTGGAGAAAGAAGCTTACCTCCAGGTGGTTGCTAAGGAGAAGCGTGAGAGAGAGGCGATGAAGCTTCTTGATGATGAGCAGA
AGCAGAAGACTGCTATGGATTTGCTAAATCAGTATCTTCAGTTTGTGC

Primers for UN54053 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 AGAGACGCTGAGCAGGAGAG 60.033 20 CCATTTAGCGCCAAGAATGT 60.096 20 273 350 622
2 CAGTCTCCCACACAAGGTGA 59.705 20 ACGCTTCTCCTTAGCAACCA 60.015 20 276 440 715
3 CAGTCTCCCACACAAGGTGA 59.705 20 CACGCTTCTCCTTAGCAACC 60.015 20 277 440 716