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Simple sequence repeat (SSR)
>UN54156
CCTGCAGCTGCCACTCTTTTCTCCTTTCCCGAAGATTAGCCGCAAAAGCTCTCCTTTGTTTCACACAACTTTCCATCCCGACAAATCACAAAAC
CGCGTCTTTTGTCTTAACGCGGGATCGTTGATTCCTCTGGATTTTCGTGAATGAATATCCAGACGGATCAGGGCGGATCTCGCGCCTTTTCGTC
CTCTCCAGCCGTATTTAGAAAGATCGAGCTTCACCCCGCGAGGAAACCCTTCAACGGCTTCTCTAATCGCGGCTCCGATTTCAAGATGGAGACT
TTGAATCCACGCTCCTCTAACAACAACAACAACAGAGCCTTCTCGTCGTCACACTCCGTTCCTGATTCTTTTGAATTGGATCGGGAGCTTACTT
TCACCAAAACCATCCGCAAAATCGGTGCTGGTTTGAAGAATCTCGGAAACACGTGTTACCTTAACTCGGTGTTGCAATGCTTGACATACACTGA
GCCGTTAGCTGCTTGCTTGACTGAAAAAAATGTGAATGGGAAAGAGACTCAGAACAATCCCCCAAGCAGTTTGGAGGCAAAAGAGGTCCTTAAG
GGGCAAAACGGTACTGCACCAGTTAAAGCAAGTGATCAAAGTGCTCCTATTGTCTTAACCC |
Primers for UN54156 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGATCGTTGATTCCTCTGGA |
60.011 |
20 |
CGGATGGTTTTGGTGAAAGT |
59.83 |
20 |
277 |
116 |
392 |
| 2 |
GGATCGTTGATTCCTCTGGA |
60.011 |
20 |
TTGGTGAAAGTAAGCTCCCG |
60.241 |
20 |
268 |
116 |
383 |
| 3 |
GGATCGTTGATTCCTCTGGA |
60.011 |
20 |
TCCCGATCCAATTCAAAAGA |
60.384 |
20 |
253 |
116 |
368 |
|
|
