 |
Simple sequence repeat (SSR)
>UN54321
GGGAGGCTAACCAGGGCTTTCTGCAATTCCTTTCTTCAGCTCTCTCTCTCTCTCTCTCTGCTCCATCTGAGGGCTAACATCTATCGCCGAAGTT
CTGTGACGAAAATGTACAGCGGATCTAGCGATGGCGAGAGCCACGACACATCGAATCAGAGGAGGATCCCTCCGGCTTCCTCGATGCTCTGGGT
TAGAAATCTACGGAGGTACATTGGTTCCGGCGCCGGTTTAGGATCCGAAGCTCTGATGGAGCTTGAGACGAAGAGAATCTTGCTGGAGATATTC
AAAGACAAGCAGCAGAAAAGTCAGGAAGCAGGCACTATACCAAGCTTCTACAAGAAGAAACCAGAAGAAGGTTCTATTAGCCAAAGAGTCCAGA
GGCTTGCTAAATACCGCTTCTTGAAGAAACAATCGGATCTTTTGTTAAATTCTGATGATTTGGCTGCTATGTGGAATTGTTTGAGAGAAAACTG
TGTGATTGATGATGGCACTGGTGCAGAGAAGATGAACTATGAAGACTTCTGTCACATTGCCTCTGTATGTACCGAACAAATCGGCCCCAAATGT
CGCCGGTTTTTCAGCCCATCCAATTTCATGAAGTTTGAGAAGGATGAGGCTGGAAGGATTGCTATTTTACCTTTCTATCTTTATGTGATGCGCA
CGGTGTCGCTTACACAGGCTAGGATTGATATGAGTGAGCTTGATGAAGATTCTGATGGTTTCCTTCAATCTCAAGAAATGGAGTCCTACATATC
TGGTCTAATACCTAATTTGGCACAAC |
Primers for UN54321 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GAGGCTAACCAGGGCTTTCT |
59.848 |
20 |
TCCTCTGATTCGATGTGTCG |
59.787 |
20 |
155 |
3 |
157 |
| 2 |
GAGGCTAACCAGGGCTTTCT |
59.848 |
20 |
TCCGCTGTACATTTTCGTCA |
60.257 |
20 |
115 |
3 |
117 |
| 3 |
AGGGCTTTCTGCAATTCCTT |
60.208 |
20 |
TCCTCTGATTCGATGTGTCG |
59.787 |
20 |
145 |
13 |
157 |
|
|
