BLASTX 7.6.2
Query= UN03000 /QuerySize=1319
(1318 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=... 322 4e-087
sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN... 320 2e-086
sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 ... 319 4e-086
sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=... 318 8e-086
sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Ni... 314 2e-084
sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE... 312 5e-084
sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 P... 311 1e-083
sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 ... 309 4e-083
sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 ... 307 1e-082
sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosacchar... 300 1e-080
sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoi... 285 8e-076
sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces ce... 261 1e-068
sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1... 161 1e-038
sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=N... 160 3e-038
sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1... 159 5e-038
sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 P... 158 1e-037
sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusio... 151 2e-035
sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusio... 141 1e-032
sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoi... 140 3e-032
sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomy... 120 3e-026
>sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=nit2a PE=2
SV=1
Length = 276
Score = 322 bits (824), Expect = 4e-087
Identities = 160/276 (57%), Positives = 199/276 (72%), Gaps = 5/276 (1%)
Frame = +1
Query: 259 LTKFNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 438
+ KF + L Q V+ KS N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60
Query: 439 IDAGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHL 618
I GE ST +LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIHL
Sbjct: 61 IP--GE---STELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIHL 115
Query: 619 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 798
FDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYDIRF ELA IYA +G LL Y
Sbjct: 116 FDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLVY 175
Query: 799 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATT 978
PGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GE++A
Sbjct: 176 PGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAKA 235
Query: 979 EHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
EE ++ AEID L + R +P+ +QRR DLY +
Sbjct: 236 GSEETVLSAEIDLQYLAEIREQIPIRRQRRRDLYNV 271
>sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN=nit2 PE=2
SV=1
Length = 276
Score = 320 bits (818), Expect = 2e-086
Identities = 157/276 (56%), Positives = 198/276 (71%), Gaps = 5/276 (1%)
Frame = +1
Query: 259 LTKFNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 438
+ KF + L Q V+ KS N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 439 IDAGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHL 618
I GE ST LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIHL
Sbjct: 61 IP--GE---STERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHL 115
Query: 619 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 798
FDID+PGKI F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA +Y+ +G LL Y
Sbjct: 116 FDIDVPGKIRFQESETLSPGDSFSVFETPYCKVGVGICYDIRFAELAQLYSKKGCQLLVY 175
Query: 799 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATT 978
PGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GE++A
Sbjct: 176 PGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKA 235
Query: 979 EHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
EE +I A+ID L + R +P+ +QRR DLY +
Sbjct: 236 GSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 271
>sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 PE=1 SV=1
Length = 276
Score = 319 bits (816), Expect = 4e-086
Identities = 160/274 (58%), Positives = 199/274 (72%), Gaps = 5/274 (1%)
Frame = +1
Query: 259 LTKFNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 438
+T F + L QL +++ KS N++ A I EAA++GAK+V LPE +NSPY FP YAE+
Sbjct: 1 MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEK 60
Query: 439 IDAGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHL 618
I GE ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIHL
Sbjct: 61 IP--GE---STQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHL 115
Query: 619 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 798
FDID+PGKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL Y
Sbjct: 116 FDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVY 175
Query: 799 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATT 978
PGAFN+TTGP HWELLQR+RA DNQ+YVAT SPARD A Y AWGHST++ P+GE+LA
Sbjct: 176 PGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKA 235
Query: 979 EHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
EEAI+ ++ID L + R +P+ +Q+R DLY
Sbjct: 236 GTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLY 269
>sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=nit2b PE=2
SV=1
Length = 276
Score = 318 bits (813), Expect = 8e-086
Identities = 157/274 (57%), Positives = 197/274 (71%), Gaps = 5/274 (1%)
Frame = +1
Query: 259 LTKFNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 438
+ KF + L Q V+ KS N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSDNLNKACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 439 IDAGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHL 618
I GE ST +LS+V+K GI +IGGSIPE + YNTC VFG DG L KHRKIHL
Sbjct: 61 IP--GE---STELLSQVAKECGIYLIGGSIPEEDSGKFYNTCAVFGPDGTLLVKHRKIHL 115
Query: 619 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 798
FDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYD+RF ELA IYA +G LL Y
Sbjct: 116 FDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDMRFAELAQIYANKGCQLLVY 175
Query: 799 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATT 978
PGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GE++A
Sbjct: 176 PGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTVVSPWGEVIAKA 235
Query: 979 EHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
EE +I A+ID L + R +P+ +QRR +LY
Sbjct: 236 GFEETVISADIDLQYLAEIREQIPIRRQRRDNLY 269
>sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Nit2 PE=1 SV=1
Length = 276
Score = 314 bits (802), Expect = 2e-084
Identities = 158/271 (58%), Positives = 193/271 (71%), Gaps = 5/271 (1%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ L QL V++ KS NI+ A + EAA +GA +V LPE +NSPY + FP YAE+I G
Sbjct: 6 LALIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEKIP--G 63
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDI 633
E ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+
Sbjct: 64 E---STKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHLFDIDV 120
Query: 634 PGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFN 813
PGKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL YPGAFN
Sbjct: 121 PGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVYPGAFN 180
Query: 814 MTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTEHEEA 993
MTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G++L EE
Sbjct: 181 MTTGPAHWELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKAGTEET 240
Query: 994 IIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
I+ ++ID L + R +P+ KQ+R DLY +
Sbjct: 241 ILYSDIDLKKLSEIRQQIPILKQKRADLYSV 271
>sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE=2 SV=1
Length = 276
Score = 312 bits (798), Expect = 5e-084
Identities = 153/271 (56%), Positives = 193/271 (71%), Gaps = 5/271 (1%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ L QL V++ KS N++ A I EA+ +GA++V LPE +NSPY FP YAE+I
Sbjct: 6 LALIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKI---- 61
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDI 633
ST LSEV+K + +IGGSIPE+ +LYNTC VFG DG L KHRK+HLFDID+
Sbjct: 62 -PGDSTQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDIDV 120
Query: 634 PGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFN 813
PGKITF ES+TL+ G++ ++ DT R+G+GICYDIRF ELA IYA RG LL YPGAFN
Sbjct: 121 PGKITFQESETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGAFN 180
Query: 814 MTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTEHEEA 993
+TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GE+LA EE
Sbjct: 181 LTTGPAHWELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTEET 240
Query: 994 IIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
I+ A+ID L + R +P+ Q+R DLY++
Sbjct: 241 IVYADIDLKKLAEIRQQIPIFSQKRSDLYEV 271
>sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 PE=2 SV=1
Length = 277
Score = 311 bits (795), Expect = 1e-083
Identities = 152/275 (55%), Positives = 197/275 (71%), Gaps = 5/275 (1%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ + QL V+ K+ N+ A+ ++EAA +GAK+V+LPE +NSPY F YAE+I G
Sbjct: 6 LAVVQLHVSKIKADNLGRAQTLVKEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKIP--G 63
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDI 633
E ST +LSE +K+ GI ++GGSIPE G +LYNTC VFG DG L HRKIHLFDID+
Sbjct: 64 E---STQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGTLLVTHRKIHLFDIDV 120
Query: 634 PGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFN 813
PGKI F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA IYA +G LL YPGAFN
Sbjct: 121 PGKIRFQESETLSPGKSLSMFETPYCKVGVGICYDIRFAELAQIYAKKGCQLLVYPGAFN 180
Query: 814 MTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTEHEEA 993
MTTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHS++I P+GE+++ EE+
Sbjct: 181 MTTGPAHWELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEES 240
Query: 994 IIIAEIDYSILEQRRTSLPLNKQRRGDLYQLVDVQ 1098
++ A+ID L R +P+ KQRR DLY + VQ
Sbjct: 241 VVYADIDLQYLADVRQQIPITKQRRNDLYSVNSVQ 275
>sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 PE=1 SV=1
Length = 276
Score = 309 bits (790), Expect = 4e-083
Identities = 155/269 (57%), Positives = 191/269 (71%), Gaps = 5/269 (1%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ L QL V++ KS N++ A + EAA +GA +V LPE +NSPY FP YAE+I G
Sbjct: 6 LALIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEKIP--G 63
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDI 633
E ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+
Sbjct: 64 E---STQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDV 120
Query: 634 PGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFN 813
PGKITF ESKTL+ G++ + DT ++G+GICYD+RF ELA IYA RG LL YPGAFN
Sbjct: 121 PGKITFQESKTLSPGDSFSTFDTPYCKVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFN 180
Query: 814 MTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTEHEEA 993
+TTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G++L EE
Sbjct: 181 LTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEET 240
Query: 994 IIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
I+ ++ID L + R +P+ KQ+R DLY
Sbjct: 241 ILYSDIDLKKLAEIRQQIPILKQKRADLY 269
>sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 PE=3 SV=1
Length = 275
Score = 307 bits (786), Expect = 1e-082
Identities = 157/269 (58%), Positives = 193/269 (71%), Gaps = 6/269 (2%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ L QL +++ S N++ A I EAA++GAK+V LPE +NSPY FP YAE+I G
Sbjct: 6 LALIQLQISSINSDNVTRACSFIREAATQGAKIVSLPECFNSPYGTKYFPEYAEKIP--G 63
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDI 633
E ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIHLFDID+
Sbjct: 64 E---STQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDV 120
Query: 634 PGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFN 813
PGKITF ESKTL+ G++ DT R+G+GICYD+RF ELA IYA RG LL YPGAFN
Sbjct: 121 PGKITFQESKTLSPGDSFCTFDT-YCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFN 179
Query: 814 MTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTEHEEA 993
+TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GE+LA EEA
Sbjct: 180 LTTGPAHWELLQRGRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEA 239
Query: 994 IIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
I+ ++ID L + R +P+ +Q+R DLY
Sbjct: 240 IVYSDIDLKKLAEIRQQIPVFRQKRSDLY 268
>sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosaccharomyces pombe
GN=SPAC26A3.11 PE=2 SV=1
Length = 322
Score = 300 bits (768), Expect = 1e-080
Identities = 149/286 (52%), Positives = 198/286 (69%), Gaps = 3/286 (1%)
Frame = +1
Query: 232 SALPLPAPPLTKFNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSN 411
SA L F IGL QL+ T DKS N+ A+ + EAA G+ +++LPEI+NSPY
Sbjct: 32 SASSLVPKDFRAFRIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGT 91
Query: 412 DSFPVYAEEIDAGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGEL 591
F YAE I+ E+SPS LS ++K + GGSIPER +LYNT VF G+L
Sbjct: 92 GYFNQYAEPIE---ESSPSYQALSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGKL 148
Query: 592 KAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYA 771
A HRKIHLFDIDIPG ++F ES +L+ G+ T+VDT+ G+ G+GICYDIRF ELAMI A
Sbjct: 149 IAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIAA 208
Query: 772 ARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIG 951
G ++ YPGAFN++TGPLHWELL RARA DN+++VA C+PARD A Y +WGHST++
Sbjct: 209 RNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVD 268
Query: 952 PFGEILATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQLV 1089
PFG+++ATT+ + +I+ A+ID S++ R S+P+ QRR D+Y V
Sbjct: 269 PFGKVIATTDEKPSIVYADIDPSVMSTARNSVPIYTQRRFDVYSEV 314
>sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoideum GN=nit2
PE=3 SV=1
Length = 328
Score = 285 bits (727), Expect = 8e-076
Identities = 143/280 (51%), Positives = 192/280 (68%), Gaps = 7/280 (2%)
Frame = +1
Query: 268 FNIGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDA 447
F QL +K N+ +A K I+EAA GAKL+ LPE +NSPYS +F Y+E D
Sbjct: 53 FKFAGIQLLCGDNKEENVQNAIKHIDEAAKNGAKLISLPECFNSPYSTSTFEKYSETED- 111
Query: 448 GGEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHRKIHLF 621
GE + LSE +KR I ++GGSIPE + ++YNTC +F GE+ KHRKIHLF
Sbjct: 112 -GE---TVKKLSEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRKIHLF 167
Query: 622 DIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYP 801
DID+P KI F ES+TLT G++ ++VD +IG+ ICYDIRF ELAM+Y+ GA L YP
Sbjct: 168 DIDVPNKIRFKESETLTPGDSFSVVDIGYCKIGVAICYDIRFPELAMLYSKMGAKFLIYP 227
Query: 802 GAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILATTE 981
GAFNM TGP HWELLQR RA DNQ++VA SPAR+ + Y AWGHST++ +G ILATT+
Sbjct: 228 GAFNMVTGPAHWELLQRGRAVDNQVFVAAISPARNPSSTYQAWGHSTIVNSWGTILATTD 287
Query: 982 HEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQLVDVQR 1101
++II ++ID + L + R+S+P+ Q+R DLY+L +++
Sbjct: 288 EHQSIIYSDIDLNTLNETRSSIPIYSQKRDDLYKLDSIKK 327
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces cerevisiae
GN=NIT3 PE=1 SV=1
Length = 291
Score = 261 bits (665), Expect = 1e-068
Identities = 137/281 (48%), Positives = 184/281 (65%), Gaps = 6/281 (2%)
Frame = +1
Query: 265 KFNIGLCQLSVTA-DKSRNISHAKKAIEEAASK--GAKLVLLPEIWNSPYSNDSFPVYAE 435
K + L QLS ++ DK N+ A IE A + KLV+LPE +NSPYS D F Y+E
Sbjct: 10 KIKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQFRKYSE 69
Query: 436 EIDAGGEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHRK 609
I+ E S S LS ++ + I ++GG+IPE D++YNT +F DG+L KHRK
Sbjct: 70 VINP-KEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHRK 128
Query: 610 IHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHL 789
+HLFD+DIP I+F ES+TL+ GE T +DT G+ G+GICYD+RF ELAM+ A +GA
Sbjct: 129 VHLFDVDIPNGISFHESETLSPGEKSTTIDTKYGKFGVGICYDMRFPELAMLSARKGAFA 188
Query: 790 LCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEIL 969
+ YP AFN TGPLHW LL R+RA DNQ+YV CSPAR+ + Y A+GHS ++ P G+I+
Sbjct: 189 MIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKIV 248
Query: 970 ATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQLVD 1092
A E II AE+D ++E R ++PL KQRR D+Y V+
Sbjct: 249 AEAGEGEEIIYAELDPEVIESFRQAVPLTKQRRFDVYSDVN 289
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1 PE=1 SV=2
Length = 327
Score = 161 bits (406), Expect = 1e-038
Identities = 94/277 (33%), Positives = 147/277 (53%), Gaps = 14/277 (5%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ +CQ++ T DK +N + + EAA GA L LPE ++ + + ++ E GG
Sbjct: 49 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSE-PLGG 107
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIHL 618
+ +++++ G+ + G ER D ++YN + S G + A +RK HL
Sbjct: 108 KLLEE---YTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHL 164
Query: 619 FDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHL 789
D++IPG+ ES + G E+P V T G+IG+ +CYD+RF EL++ A GA +
Sbjct: 165 CDVEIPGQGPMCESNSTMPGPSLESP--VSTPAGKIGLAVCYDMRFPELSLALAQAGAEI 222
Query: 790 LCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEIL 969
L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G ++
Sbjct: 223 LTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVV 282
Query: 970 ATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
A + +A ID + L Q R LP+ + RR DLY
Sbjct: 283 ARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 319
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=Nit1 PE=2 SV=1
Length = 292
Score = 160 bits (403), Expect = 3e-038
Identities = 98/280 (35%), Positives = 146/280 (52%), Gaps = 20/280 (7%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ +CQ++ T +K N + ++EA GA L LPE ++ N + + E G
Sbjct: 14 VAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLLSEPLDG- 72
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIHL 618
S++++ GI + G ER D ++YN + S G + A +RK HL
Sbjct: 73 ---DLLGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTHL 129
Query: 619 FDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHL 789
D++IPG+ ES G E P V T G++G+ ICYD+RF EL++ A GA +
Sbjct: 130 CDVEIPGQGPMRESNYTMPGYALEPP--VKTPAGKVGLAICYDMRFPELSLKLAQAGAEI 187
Query: 790 LCYPGAFNMTTGPLHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFG 960
L YP AF TGP HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+G
Sbjct: 188 LTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPWG 244
Query: 961 EILATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
++A+ + +A ID L+Q R LP+ + RR DLY
Sbjct: 245 TVVASCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 284
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1 PE=2 SV=1
Length = 323
Score = 159 bits (401), Expect = 5e-038
Identities = 97/278 (34%), Positives = 144/278 (51%), Gaps = 16/278 (5%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ +CQ++ T +K N + ++EAA GA L LPE ++ N + + E G
Sbjct: 45 VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPLNG- 103
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIHL 618
S++++ GI + G ER D ++YN + S G + A +RK HL
Sbjct: 104 ---DLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHL 160
Query: 619 FDIDIPGKITFMESK-TLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 795
D++IPG+ ES T G V T G++G+ ICYD+RF EL++ A GA +L
Sbjct: 161 CDVEIPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGAEILT 220
Query: 796 YPGAFNMTTGPLHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFGEI 966
Y AF TGP HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+G +
Sbjct: 221 YSSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPWGTV 277
Query: 967 LATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
+A + +A ID L+Q R LP+ + RR DLY
Sbjct: 278 VARCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 315
>sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 PE=2 SV=1
Length = 328
Score = 158 bits (398), Expect = 1e-037
Identities = 93/277 (33%), Positives = 143/277 (51%), Gaps = 14/277 (5%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 453
+ +CQ++ T DK +N + I EAA GA L LPE ++ + E +G
Sbjct: 50 VAVCQVTSTPDKEQNFKTCAELIREAARLGACLAFLPEAFDFIARDPEETRRLSEPLSGN 109
Query: 454 EASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIHL 618
T ++++ G+ + G ER D ++YN + + G + A +RK HL
Sbjct: 110 LLEEYT----QLARECGLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTHL 165
Query: 619 FDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHL 789
D++IPG+ ES + G E+P + T G+IG+ ICYD+RF EL++ GA +
Sbjct: 166 CDVEIPGQGPMRESNSTIPGPSLESP--ISTPAGKIGLAICYDMRFPELSLALVQAGAEI 223
Query: 790 LCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEIL 969
L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G ++
Sbjct: 224 LTYPSAFGSVTGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVV 283
Query: 970 ATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLY 1080
A + +A ID + L+Q R LP+ + RR DLY
Sbjct: 284 ARCSEGPGLCLARIDLNYLQQLRKQLPVFQHRRPDLY 320
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Caenorhabditis elegans GN=nft-1 PE=1 SV=1
Length = 440
Score = 151 bits (379), Expect = 2e-035
Identities = 76/179 (42%), Positives = 107/179 (59%), Gaps = 1/179 (0%)
Frame = +1
Query: 553 YNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAG-ETPTIVDTDVGRIGIGI 729
+NT + SDG +A++ K+HLFD++IPGK+ MES+ AG E VDT +GR+G+ I
Sbjct: 109 WNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSI 168
Query: 730 CYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDS 909
CYD+RF EL++ RGA LL +P AF + TG HWE L RARA +NQ YV +
Sbjct: 169 CYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAH 228
Query: 910 GAGYTAWGHSTLIGPFGEILATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
++GHS ++ P+G ++A + AEID S ++ R P+ RR DLY L
Sbjct: 229 NPKRQSYGHSMVVDPWGAVVAQCSERVDMCFAEIDLSYVDTLREMQPVFSHRRSDLYTL 287
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Drosophila melanogaster GN=NitFhit PE=1 SV=1
Length = 460
Score = 141 bits (355), Expect = 1e-032
Identities = 83/223 (37%), Positives = 122/223 (54%), Gaps = 7/223 (3%)
Frame = +1
Query: 472 AMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITF 651
A E++K I I G + ER +++N + GEL A +RK+H+FD+ ++
Sbjct: 97 AQYRELAKCNKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTK-EVRL 155
Query: 652 MESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTT 822
ES T+T G E P V T VG+IG+ ICYD+RF E A++ GA+LL YP AF T
Sbjct: 156 RESDTVTPGYCLERP--VSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYAT 213
Query: 823 GPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEILA-TTEHEEAII 999
G HWE+L RARA + Q +V + +WGHS ++ P+G +LA +E E I
Sbjct: 214 GKAHWEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIG 273
Query: 1000 IAEIDYSILEQRRTSLPLNKQRRGDLYQLVDVQRLNSK*VQQR 1128
AE+D S+L+ ++P + RR D+Y L + + Q R
Sbjct: 274 TAEVDLSVLQSLYQTMPCFEHRRNDIYALTAYNLRSKEPTQDR 316
>sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoideum GN=nit1-1
PE=3 SV=1
Length = 291
Score = 140 bits (351), Expect = 3e-032
Identities = 88/284 (30%), Positives = 147/284 (51%), Gaps = 20/284 (7%)
Frame = +1
Query: 274 IGLCQLSVTADKSRNISHAKKAIEEAASKGAKLVLLPEIW-------NSPYSNDSFPVYA 432
IGL Q++ T +K N K+ IE+A L LPE + + S D+ A
Sbjct: 15 IGLGQITSTNNKEDNFRKCKEMIEKAVENKVNLFCLPECFAFISGGIHQFESRDN----A 70
Query: 433 EEIDAGGEASPSTAMLSEVSKRLGITIIGGSIPERV----GDRLYNTCCVFGSDGELKAK 600
E +D G +++K+ I + G E++ D +YNT + S+G + +
Sbjct: 71 EYLDQKGGI---IERYKDLAKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIVCE 127
Query: 601 HRKIHLFDIDIPGK-ITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAAR 777
+RK+HLFD+DIP K + ESK + G + D+ VG++G+ ICYD+RF EL +
Sbjct: 128 YRKMHLFDVDIPSKGVKMNESKVVKGGNDLVVCDSPVGKLGLSICYDLRFPELYLSLRRM 187
Query: 778 GAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPF 957
A +L P AF +TG HW+ L +ARA +NQ YV + D + +++GHS +I P+
Sbjct: 188 DAQILLVPSAFMKSTGEAHWKPLLQARAIENQTYVIAAAQTGDHHSKRSSYGHSMIIDPW 247
Query: 958 GEIL-ATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQL 1086
G++L ++ I +ID + R ++P+ ++ + Y++
Sbjct: 248 GKVLHDLPDNLNDIAFVDIDLDYISTCRENIPVFNHKKLNNYKI 291
>sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomyces pombe
GN=nit2 PE=2 SV=1
Length = 276
Score = 120 bits (299), Expect = 3e-026
Identities = 68/192 (35%), Positives = 105/192 (54%), Gaps = 7/192 (3%)
Frame = +1
Query: 529 PERVGDRLYNTCC-VFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDT 702
P +V ++L N+ + GE+ +++ K HLFD++I T ES T GE T
Sbjct: 87 PSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRGEAILPPCKT 146
Query: 703 DVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYV 882
+G++G IC+DIRF E A+ GAH++ YP AF TG HWE+L RARA D+Q YV
Sbjct: 147 PLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAHWEVLLRARALDSQCYV 206
Query: 883 ATCSPARDSGAGYTAWGHSTLIGPFGEILA---TTEHEEAIIIAEIDYSILEQRRTSLPL 1053
+ ++GHS ++ P+G ++A +I A++D ++++ RT +PL
Sbjct: 207 IAPAQGGKHNEKRASYGHSMIVDPWGTVIAQYSDISSPNGLIFADLDLNLVDHVRTYIPL 266
Query: 1054 NKQRRGDLYQLV 1089
RR DLY +
Sbjct: 267 --LRRNDLYPTI 276
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,533,745,664
Number of Sequences: 518415
Number of Extensions: 12533745664
Number of Successful Extensions: 80211091
Number of sequences better than 0.0: 0
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