BLASTX 7.6.2
Query= UN04673 /QuerySize=1210
(1209 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine synthase 3-like OS=Ara... 234 1e-060
sp|Q7XT07|DUS3L_ORYSJ tRNA-dihydrouridine synthase 3-like OS=Ory... 121 1e-026
sp|Q7ZWS1|DUS3L_XENLA tRNA-dihydrouridine synthase 3-like OS=Xen... 81 2e-014
sp|Q28BT8|DUS3L_XENTR tRNA-dihydrouridine synthase 3-like OS=Xen... 80 3e-014
sp|Q96G46|DUS3L_HUMAN tRNA-dihydrouridine synthase 3-like OS=Hom... 76 5e-013
sp|Q3KRC5|DUS3L_RAT tRNA-dihydrouridine synthase 3-like OS=Rattu... 73 4e-012
sp|Q91XI1|DUS3L_MOUSE tRNA-dihydrouridine synthase 3-like OS=Mus... 72 9e-012
sp|Q6C4K3|DUS3_YARLI tRNA-dihydrouridine synthase 3 OS=Yarrowia ... 69 5e-011
sp|Q54CU9|DUS3L_DICDI tRNA-dihydrouridine synthase 3-like OS=Dic... 65 1e-009
sp|A8NZY7|DUS3_COPC7 tRNA-dihydrouridine synthase 3 OS=Coprinops... 65 1e-009
sp|Q9UTH9|DUS3_SCHPO tRNA-dihydrouridine synthase 3 OS=Schizosac... 59 6e-008
sp|Q4P1U2|DUS3_USTMA tRNA-dihydrouridine synthase 3 OS=Ustilago ... 58 1e-007
sp|A8PTG4|DUS3_MALGO tRNA-dihydrouridine synthase 3 OS=Malassezi... 56 4e-007
sp|A3LUK5|DUS3_PICST tRNA-dihydrouridine synthase 3 OS=Pichia st... 53 3e-006
sp|Q6FJ14|DUS3_CANGA tRNA-dihydrouridine synthase 3 OS=Candida g... 53 5e-006
>sp|Q9T0J6|DUS3L_ARATH tRNA-dihydrouridine synthase 3-like OS=Arabidopsis
thaliana GN=At4g38890 PE=2 SV=1
Length = 700
Score = 234 bits (596), Expect = 1e-060
Identities = 138/247 (55%), Positives = 151/247 (61%), Gaps = 66/247 (26%)
Frame = +2
Query: 545 SDENQISEINVFNKETQRLLWKNNMTFLKADAKLKSLGLMVSCT*YASCFFD*LPYLLPW 724
SD+ + SE+N FNKETQRLLWKN MTF ADAKLKSLGL+
Sbjct: 176 SDDKEKSEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLL-------------------- 215
Query: 725 PWDMNISLPLFVSLFPYHF*GHAKKSNVAEENDAHKPQ---NDAQATE------------ 859
GHAKKSN AEE A K Q N QATE
Sbjct: 216 --------------------GHAKKSNAAEEITAEKTQNGMNGTQATEVAVDSAVSSEHT 255
Query: 860 -----GVDIPGPLETEQVRPAKRAKSE------TSQLGDINIGVNVEDNPEKNGHSTSKP 1006
VDIPGPLETE+VRP K+AKSE T +G + GV +E+ +KNG+ TSK
Sbjct: 256 SEMIQDVDIPGPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKA 315
Query: 1007 KVEDEASIKVVETDGSLKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTC 1186
VEDE SIK+VETD SLK HPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL ADVTC
Sbjct: 316 NVEDEDSIKIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTC 375
Query: 1187 GEMAMCT 1207
GEMAMCT
Sbjct: 376 GEMAMCT 382
Score = 125 bits (312), Expect = 9e-028
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = +3
Query: 345 INLCPQVSRTEDVNSCQYKEKCRFNHDIEAFKSQKPDDIEGQCPFVASGMKCQYGLSCRF 524
INLCPQV+RTEDV+SCQYK+KCRFNHDIEAFK+QK DDIEGQCPFVASGMKC YGLSCRF
Sbjct: 106 INLCPQVARTEDVDSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRF 165
Query: 525 L 527
L
Sbjct: 166 L 166
Score = 123 bits (308), Expect = 3e-027
Identities = 68/89 (76%), Positives = 73/89 (82%), Gaps = 2/89 (2%)
Frame = +3
Query: 72 AELAAETSVKEDAKAVLETVQSTKQFSVYEASSEELIERSMAPIKKEFLCPPPPSRNPKQ 251
AE A +TSV+ DAKA L VQSTKQFSVYEA+SEELIERSMAPIKKEFLCPPPPSR+ KQ
Sbjct: 6 AEHAVDTSVEGDAKAAL-PVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPPPSRSVKQ 64
Query: 252 SD-VKAAPPHPSLVQEKKSKRQLKRERRE 335
+D P LVQEKKSKRQLKRERRE
Sbjct: 65 NDAADVRAPQSGLVQEKKSKRQLKRERRE 93
>sp|Q7XT07|DUS3L_ORYSJ tRNA-dihydrouridine synthase 3-like OS=Oryza sativa
subsp. japonica GN=Os04g0117600 PE=2 SV=2
Length = 685
Score = 121 bits (302), Expect = 1e-026
Identities = 63/127 (49%), Positives = 87/127 (68%), Gaps = 5/127 (3%)
Frame = +3
Query: 165 SSEELIERSMAPIKKEFLCPPPPSRNPKQSDVKAAPPHPSLVQEKKSKRQLKRERR--EK 338
S EEL+ R++AP+K FL PP + PK D A ++V EKKSKRQLKRER+ +K
Sbjct: 30 SPEELVARAVAPVKPAFLRPPLSATPPK--DEGKANGGGAVVAEKKSKRQLKRERKQEQK 87
Query: 339 CAINLCPQVSRTEDVNSCQYKEKCRFNHDIEAFKSQKPDDIEGQCPFVASGMKCQYGLSC 518
+ +LC +V ++ +V+SC+Y + CRF+HDI+A+ +QKP D+EG CPF C YGL+C
Sbjct: 88 SSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPADLEGTCPFTNLDQLCPYGLTC 147
Query: 519 RFLALTH 539
RFL TH
Sbjct: 148 RFLG-TH 153
Score = 96 bits (236), Expect = 6e-019
Identities = 55/108 (50%), Positives = 66/108 (61%), Gaps = 13/108 (12%)
Frame = +2
Query: 899 RPAKRAKSETSQL-----GDINIGVNVEDNPEKNGHSTSKPKVEDEASIKVVETDGSLKS 1063
RP K++K E+ ++ G +N ED NG +E + TD
Sbjct: 268 RPTKKSKVESDEIDKHGAGTLNTNTESEDPNLSNG-------LEPSNNSSSCRTDLITTP 320
Query: 1064 HPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMCT 1207
H RE KK+IDFR+KLYLAPLTTVGNLPFRRLCK L AD+TCGEMAMCT
Sbjct: 321 HLRE-KKIIDFREKLYLAPLTTVGNLPFRRLCKTLGADITCGEMAMCT 367
>sp|Q7ZWS1|DUS3L_XENLA tRNA-dihydrouridine synthase 3-like OS=Xenopus laevis
GN=dus3l PE=2 SV=1
Length = 640
Score = 81 bits (198), Expect = 2e-014
Identities = 40/61 (65%), Positives = 47/61 (77%), Gaps = 1/61 (1%)
Frame = +2
Query: 1025 SIKVVETDGSLKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMC 1204
++ V + +K P E KK IDFR+KLYLAPLTT GNLPFRR+CK L AD+TCGEMAMC
Sbjct: 271 TVGAVTDEDVIKLRPCE-KKTIDFRNKLYLAPLTTCGNLPFRRICKRLGADITCGEMAMC 329
Query: 1205 T 1207
T
Sbjct: 330 T 330
Score = 62 bits (149), Expect = 7e-009
Identities = 31/83 (37%), Positives = 51/83 (61%), Gaps = 4/83 (4%)
Frame = +3
Query: 294 EKKSKRQLKRERREKCAINLCPQVSRTEDVNSCQYKEKCRFNHDIEAFKSQKPDDIEGQC 473
+ KS+ +K + E+ LCP V++ E + C + +KC+F+HD+ + SQKP+DI C
Sbjct: 111 QNKSRPHMKHSQFEE--NKLCPSVTQ-ECASKCFFGDKCKFSHDVAKYVSQKPEDIRPNC 167
Query: 474 PFVASGMKCQYGLSCRFLALTHM 542
+ KC YG++CRF A +H+
Sbjct: 168 HLYETFGKCIYGVTCRF-AKSHL 189
>sp|Q28BT8|DUS3L_XENTR tRNA-dihydrouridine synthase 3-like OS=Xenopus tropicalis
GN=dus3l PE=2 SV=2
Length = 639
Score = 80 bits (195), Expect = 3e-014
Identities = 40/61 (65%), Positives = 46/61 (75%), Gaps = 1/61 (1%)
Frame = +2
Query: 1025 SIKVVETDGSLKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMC 1204
++ V + +K P E KK IDFR+KLYLAPLTT GNLPFRRLCK AD+TCGEMAMC
Sbjct: 270 TVGAVTDEDLVKLRPCE-KKTIDFRNKLYLAPLTTCGNLPFRRLCKRFGADITCGEMAMC 328
Query: 1205 T 1207
T
Sbjct: 329 T 329
>sp|Q96G46|DUS3L_HUMAN tRNA-dihydrouridine synthase 3-like OS=Homo sapiens
GN=DUS3L PE=1 SV=2
Length = 650
Score = 76 bits (185), Expect = 5e-013
Identities = 40/74 (54%), Positives = 48/74 (64%), Gaps = 1/74 (1%)
Frame = +2
Query: 986 GHSTSKPKVEDEASIKVVETDGSLKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKV 1165
G TS P + + + ++ P EKK+L D R KLYLAPLTT GNLPFRR+CK
Sbjct: 268 GPGTSTPPSSPVRTCGPLTDEDVVRLRPCEKKRL-DIRGKLYLAPLTTCGNLPFRRICKR 326
Query: 1166 L*ADVTCGEMAMCT 1207
ADVTCGEMA+CT
Sbjct: 327 FGADVTCGEMAVCT 340
>sp|Q3KRC5|DUS3L_RAT tRNA-dihydrouridine synthase 3-like OS=Rattus norvegicus
GN=Dus3l PE=2 SV=1
Length = 640
Score = 73 bits (177), Expect = 4e-012
Identities = 36/51 (70%), Positives = 40/51 (78%), Gaps = 1/51 (1%)
Frame = +2
Query: 1055 LKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMCT 1207
++ P EKK+L D KLYLAPLTT GNLPFRR+CK ADVTCGEMAMCT
Sbjct: 281 VRLRPCEKKRL-DISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAMCT 330
>sp|Q91XI1|DUS3L_MOUSE tRNA-dihydrouridine synthase 3-like OS=Mus musculus
GN=Dus3l PE=2 SV=1
Length = 637
Score = 72 bits (174), Expect = 9e-012
Identities = 35/51 (68%), Positives = 40/51 (78%), Gaps = 1/51 (1%)
Frame = +2
Query: 1055 LKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMCT 1207
++ P EKK+L D KLYLAPLTT GNLPFRR+CK ADVTCGEMA+CT
Sbjct: 278 IRLRPCEKKRL-DISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCT 327
>sp|Q6C4K3|DUS3_YARLI tRNA-dihydrouridine synthase 3 OS=Yarrowia lipolytica
GN=DUS3 PE=3 SV=1
Length = 622
Score = 69 bits (168), Expect = 5e-011
Identities = 49/153 (32%), Positives = 70/153 (45%), Gaps = 19/153 (12%)
Frame = +3
Query: 111 KAVLETVQSTKQFSVYEASSEELIERSMAPIKKEFLC--------PPPPSRNPKQSDVKA 266
K +E + T SVYE E R +A IK E+L PPS N ++
Sbjct: 9 KTKVEDLPDTIVDSVYEKQLAEGRVRGVASIKPEYLVARASADARDAPPSVNDDLAEGGE 68
Query: 267 APPHPSLVQEKKSKRQLKRE--RREKCAINLCPQVSRTEDVNS---CQYKEKCRFNHDIE 431
P +EKK Q K ++ K AI +C + ++ S C Y +KC+F HD++
Sbjct: 69 KP------EEKKKGGQNKNRSLKQRKDAIKMCSSALQFKEDGSPQECSYGDKCKFEHDLQ 122
Query: 432 AFKSQKPDDIEGQCPFVASGMKCQYGLSCRFLA 530
+ KP DIEG CP +C G CR+L+
Sbjct: 123 KYLESKPKDIEGVCPVFDLTGQCPQGYKCRWLS 155
Score = 58 bits (139), Expect = 1e-007
Identities = 38/104 (36%), Positives = 59/104 (56%), Gaps = 8/104 (7%)
Frame = +2
Query: 890 EQVRPAKRAKSETSQLGDINIGVNVEDNPEKNGHSTSKPKVEDEASIKVVETDGSLKSHP 1069
+ + P +R K + S+ + + + D + K+E + +I++ + + P
Sbjct: 185 KHLNPLQRKKYDLSKSDE---AIKLIDAQIVRDEDDEQTKLEKKETIEI----DTGRIRP 237
Query: 1070 REKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAM 1201
EKKKL D + K ++PLTTVGNLP+RRL K L ADVT GEMA+
Sbjct: 238 SEKKKL-DLKGKYIVSPLTTVGNLPYRRLMKDLGADVTYGEMAL 280
>sp|Q54CU9|DUS3L_DICDI tRNA-dihydrouridine synthase 3-like OS=Dictyostelium
discoideum GN=dus3l PE=3 SV=1
Length = 671
Score = 65 bits (156), Expect = 1e-009
Identities = 37/72 (51%), Positives = 47/72 (65%), Gaps = 1/72 (1%)
Frame = +2
Query: 992 STSKPKVEDEASIKVVETDGSLKSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL* 1171
+T K E + S + E D EKK+ I+F ++L LAPLTTVGNLPFRR+CK L
Sbjct: 289 TTETKKEEFKQSNENTEIDIMTPLKANEKKR-INFENQLILAPLTTVGNLPFRRICKRLG 347
Query: 1172 ADVTCGEMAMCT 1207
AD+T GEMA+ T
Sbjct: 348 ADITIGEMALTT 359
>sp|A8NZY7|DUS3_COPC7 tRNA-dihydrouridine synthase 3 OS=Coprinopsis cinerea
(strain Okayama-7 / 130 / FGSC 9003) GN=DUS3 PE=3 SV=1
Length = 676
Score = 65 bits (156), Expect = 1e-009
Identities = 28/44 (63%), Positives = 36/44 (81%)
Frame = +2
Query: 1076 KKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMCT 1207
+K+ +D++ K YLAPLTTVGNLPFRR+C L AD+TCGEM + T
Sbjct: 303 EKRRLDWKGKTYLAPLTTVGNLPFRRMCVKLGADITCGEMGLAT 346
>sp|Q9UTH9|DUS3_SCHPO tRNA-dihydrouridine synthase 3 OS=Schizosaccharomyces
pombe GN=dus3 PE=2 SV=1
Length = 617
Score = 59 bits (141), Expect = 6e-008
Identities = 29/44 (65%), Positives = 33/44 (75%)
Frame = +2
Query: 1073 EKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAMC 1204
++K LID+RD+ LAPLTTVGN PFRRLC L AD EMAMC
Sbjct: 230 QEKNLIDWRDRKILAPLTTVGNPPFRRLCGSLGADTFYSEMAMC 273
>sp|Q4P1U2|DUS3_USTMA tRNA-dihydrouridine synthase 3 OS=Ustilago maydis GN=DUS3
PE=3 SV=1
Length = 867
Score = 58 bits (139), Expect = 1e-007
Identities = 29/56 (51%), Positives = 41/56 (73%), Gaps = 4/56 (7%)
Frame = +2
Query: 1043 TDGSL---KSHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAM 1201
T+GS+ + P EK++L ++R +LYLAPLTT GNLPFRR+C +D+ CGEM +
Sbjct: 469 TNGSIDLARIRPSEKRRL-NWRGELYLAPLTTTGNLPFRRICTGYGSDIHCGEMGL 523
>sp|A8PTG4|DUS3_MALGO tRNA-dihydrouridine synthase 3 OS=Malassezia globosa
(strain ATCC 96807 / CBS 7966) GN=DUS3 PE=3 SV=1
Length = 766
Score = 56 bits (134), Expect = 4e-007
Identities = 27/45 (60%), Positives = 33/45 (73%), Gaps = 1/45 (2%)
Frame = +2
Query: 1067 PREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAM 1201
P EK++L ++ LYLAPLTT GNLPFRRLC +D+ CGEM M
Sbjct: 385 PCEKRRL-QWKGDLYLAPLTTTGNLPFRRLCASFGSDIHCGEMGM 428
>sp|A3LUK5|DUS3_PICST tRNA-dihydrouridine synthase 3 OS=Pichia stipitis GN=DUS3
PE=3 SV=1
Length = 613
Score = 53 bits (126), Expect = 3e-006
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = +2
Query: 1076 KKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAM 1201
+KK +DFR+ ++PLTTVGNLP+RRL K L AD+T EMA+
Sbjct: 236 EKKRLDFRNAKIVSPLTTVGNLPYRRLMKKLGADITYSEMAL 277
>sp|Q6FJ14|DUS3_CANGA tRNA-dihydrouridine synthase 3 OS=Candida glabrata GN=DUS3
PE=3 SV=1
Length = 640
Score = 53 bits (125), Expect = 5e-006
Identities = 25/43 (58%), Positives = 33/43 (76%)
Frame = +2
Query: 1073 EKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVL*ADVTCGEMAM 1201
++KK +D R K ++PLTTVGNLP+RRL + L ADVT EMA+
Sbjct: 258 QEKKPLDLRHKKIVSPLTTVGNLPYRRLMRTLGADVTYSEMAL 300
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,655,569,443
Number of Sequences: 518415
Number of Extensions: 18655569443
Number of Successful Extensions: 134721190
Number of sequences better than 0.0: 0
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