BLASTX 7.6.2
Query= UN07061 /QuerySize=2074
(2073 letters)
Database: UniProt/TrEMBL;
11,397,958 sequences; 3,661,877,547 total letters
Score E
Sequences producing significant alignments: (bits) Value
tr|Q8VZE1|Q8VZE1_ARATH At2g17250/T23A1.11 OS=Arabidopsis thalian... 835 3e-240
tr|B9HPX1|B9HPX1_POPTR Predicted protein (Fragment) OS=Populus t... 640 2e-181
tr|B9SYR1|B9SYR1_RICCO Nucleolar complex protein, putative OS=Ri... 573 4e-161
tr|Q01K64|Q01K64_ORYSA H0307D04.2 protein OS=Oryza sativa GN=H03... 377 3e-102
tr|Q7XP58|Q7XP58_ORYSJ OSJNBa0013K16.9 protein OS=Oryza sativa s... 377 3e-102
tr|C6TAQ8|C6TAQ8_SOYBN Putative uncharacterized protein OS=Glyci... 360 6e-097
tr|A9RUI9|A9RUI9_PHYPA Predicted protein OS=Physcomitrella paten... 326 7e-087
tr|B6SWX2|B6SWX2_MAIZE Nucleolar complex protein 4 OS=Zea mays P... 283 9e-074
tr|C4IY33|C4IY33_MAIZE Putative uncharacterized protein OS=Zea m... 283 9e-074
tr|B8ATD3|B8ATD3_ORYSI Putative uncharacterized protein OS=Oryza... 280 6e-073
tr|B9FC62|B9FC62_ORYSJ Putative uncharacterized protein OS=Oryza... 280 6e-073
tr|Q0JAP6|Q0JAP6_ORYSJ Os04g0585300 protein OS=Oryza sativa subs... 280 6e-073
tr|C5YEL7|C5YEL7_SORBI Putative uncharacterized protein Sb06g026... 275 2e-071
tr|C1N5W2|C1N5W2_9CHLO Predicted protein OS=Micromonas pusilla C... 188 3e-045
tr|Q00UD0|Q00UD0_OSTTA Predicted nucleolar protein involved in r... 188 3e-045
tr|D2HLG5|D2HLG5_AILME Putative uncharacterized protein (Fragmen... 185 2e-044
tr|Q4T6B1|Q4T6B1_TETNG Chromosome undetermined SCAF8839, whole g... 184 5e-044
tr|Q3UKH4|Q3UKH4_MOUSE Putative uncharacterized protein OS=Mus m... 182 2e-043
>tr|Q8VZE1|Q8VZE1_ARATH At2g17250/T23A1.11 OS=Arabidopsis thaliana GN=At2g17250
PE=2 SV=1
Length = 577
Score = 835 bits (2157), Expect = 3e-240
Identities = 441/547 (80%), Positives = 474/547 (86%), Gaps = 20/547 (3%)
Frame = -3
Query: 1810 LLSQLPPSSPPP---------SSTKRPRSGEEEEEDDSSSKTKTADEVDGDPEVVLRAWL 1658
LL+ + P SPP S P + S S TKT DPEVV +AWL
Sbjct: 40 LLTFVSPESPPQFVVESLLSLQSFFTPLLSQLPPTSSSPSSTKTE-----DPEVVFKAWL 94
Query: 1657 RSKFDEFVKLLLDVLVSQQSEDTLRDIVLGTLMEFVKLLNAGRFHSSIYHRILNAIIHSA 1478
RSKFDEFVKLLLDVLVSQQSED+LR IVLGTLMEFVKLLNAGRFHSSIYHR+L+AIIHS
Sbjct: 95 RSKFDEFVKLLLDVLVSQQSEDSLRGIVLGTLMEFVKLLNAGRFHSSIYHRLLDAIIHSE 154
Query: 1477 VDADIFLDILNSNYFKYIDVRYFTYISMEKFVKTLEASAVSADRTVNENSETENESKDSL 1298
VD +IFLDIL S YFKYIDVRYFTYISMEKFVKTLEAS VSADRTV EN+E E++SK+SL
Sbjct: 155 VDIEIFLDILTSKYFKYIDVRYFTYISMEKFVKTLEAS-VSADRTVIENNEAESDSKESL 213
Query: 1297 EHSIRKIYQVLSRTPPPQKQAEKSGHEMWSGSDESSSEKPKDKKKKKNKDQDSNLLSPTT 1118
E S+RKIYQVLS+ PPP+KQAEKS HEMWSGSDES SEKP DKKKK K DS LLSP T
Sbjct: 214 ELSVRKIYQVLSQIPPPEKQAEKSQHEMWSGSDESISEKPTDKKKKTEKG-DSTLLSPAT 272
Query: 1117 IAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGV 938
I+KRMKLKFTKAWISFLRLPLP+DVYKEVLASIH TVIPHLSNP MLCDFLTKSYDIGGV
Sbjct: 273 ISKRMKLKFTKAWISFLRLPLPIDVYKEVLASIHLTVIPHLSNPTMLCDFLTKSYDIGGV 332
Query: 937 VSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAY 758
VSVMALSSLFILMT HGLEYP FYEKLYALLVPSVFVAKHRA+FLQLLDACLKSS+LPAY
Sbjct: 333 VSVMALSSLFILMTRHGLEYPFFYEKLYALLVPSVFVAKHRAKFLQLLDACLKSSMLPAY 392
Query: 757 LAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVENANEANSEADED 578
LAASF KKLSRLSLS+PP+GSLVITALIYNLLRR+PTINHLV QE VENA+EAN+EA E
Sbjct: 393 LAASFTKKLSRLSLSIPPAGSLVITALIYNLLRRNPTINHLV-QEIVENADEANTEAGEH 451
Query: 577 NESSRPKT--NKKLGIDYFNNQETDLKKTGAMKSSLWEIDTLRHHYCPPVSRFVSSLETD 404
NE S+PKT +KLGIDYFNNQE+D KK+GA+KSSLWEIDTLRHHYCPPVSRF+SSLET+
Sbjct: 452 NE-SQPKTIKKRKLGIDYFNNQESDPKKSGALKSSLWEIDTLRHHYCPPVSRFISSLETN 510
Query: 403 LTIRAKTTEMKIEDFSSGSYATIFGDEIRRRVKQVPLAFYKAVPTSLFEDSDFPGWTFNI 224
LTIR+KTTEMKIEDF SGSYATIFGDEIRRRVKQVPLAFYK VPTSLF DSDFPGWTF I
Sbjct: 511 LTIRSKTTEMKIEDFCSGSYATIFGDEIRRRVKQVPLAFYKTVPTSLFADSDFPGWTFTI 570
Query: 223 PQDEGKC 203
PQ+EG C
Sbjct: 571 PQEEGTC 577
>tr|B9HPX1|B9HPX1_POPTR Predicted protein (Fragment) OS=Populus trichocarpa
GN=POPTRDRAFT_199710 PE=4 SV=1
Length = 575
Score = 640 bits (1650), Expect = 2e-181
Identities = 322/504 (63%), Positives = 398/504 (78%), Gaps = 6/504 (1%)
Frame = -3
Query: 1726 SSSKTKTADEVDGDPEVVLRAWLRSKFDEFVKLLLDVLVSQQSEDTLRDIVLGTLMEFVK 1547
S S T D + D +V+ + WLRSKFDEFVK L+DV VS ++ED L+++VL TLMEFVK
Sbjct: 68 SRSSTHNKDNDEPDADVIYKTWLRSKFDEFVKSLVDVAVSPKAEDALKEVVLDTLMEFVK 127
Query: 1546 LLNAGRFHSSIYHRILNAIIHSAVDADIFLDILNSNYFKYIDVRYFTYISMEKFVKTLEA 1367
N GRF+S+IYHR L I+ S D L++L S YFKYID+RYFTYI++EKF K LE
Sbjct: 128 TGNGGRFNSAIYHRFLVNIVQSTESLDFVLELLASKYFKYIDIRYFTYINIEKFAKNLEL 187
Query: 1366 SAVSADRTVNENSETENESKDSLEHSIRKIYQVLSRTPPPQKQAEKSGHEMWSGSDESSS 1187
+S +T + + E++S++SLE SI KI+ ++S PP + + S +E+W GS S
Sbjct: 188 KDISDGKTESGDKVGESDSRESLELSIYKIHYIISNIPPLEDPKQNSDYELWGGSGPSQH 247
Query: 1186 EKPKDKKKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTV 1007
K +DK K K D+++LS AK+MKLKFTKAWISFLRLPLP+DVYKEVL+++HQ V
Sbjct: 248 LKTEDKDLKSEK-HDNDVLSAGNYAKKMKLKFTKAWISFLRLPLPIDVYKEVLSNLHQAV 306
Query: 1006 IPHLSNPAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFV 827
IPHLSNP MLCDFLT+SYDIGGVVSVMALSSLFILMT+HGLEYPNFYEKLY LL+PS+F+
Sbjct: 307 IPHLSNPIMLCDFLTRSYDIGGVVSVMALSSLFILMTKHGLEYPNFYEKLYVLLLPSIFM 366
Query: 826 AKHRARFLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPT 647
AKHRA+F QLLD+CLKS LLPAYLAA+FAKKLSRL+L VPPSG+LVI ALI+NLLRRHP+
Sbjct: 367 AKHRAKFFQLLDSCLKSPLLPAYLAAAFAKKLSRLALVVPPSGALVIIALIHNLLRRHPS 426
Query: 646 INHLVHQEPVENANEANSEA---DEDNE--SSRPKTNKKLGIDYFNNQETDLKKTGAMKS 482
IN LVHQE + + NSEA D +NE +S +K GID+F+N+E++ K+ A+ S
Sbjct: 427 INCLVHQEDCNDTTDNNSEAEGGDNENEFGASTNIAARKAGIDHFDNEESNPLKSHALGS 486
Query: 481 SLWEIDTLRHHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIFGDEIRRRVKQ 302
SLWEID+LRHHYCPPVSRFV SLE DLT+RAKTTE+ +EDFSSGSYATIFG+EIRRRVKQ
Sbjct: 487 SLWEIDSLRHHYCPPVSRFVQSLENDLTVRAKTTEVNVEDFSSGSYATIFGEEIRRRVKQ 546
Query: 301 VPLAFYKAVPTSLFEDSDFPGWTF 230
VP+AFYKA+PTSLF ++DF GW+F
Sbjct: 547 VPVAFYKAIPTSLFSETDFSGWSF 570
>tr|B9SYR1|B9SYR1_RICCO Nucleolar complex protein, putative OS=Ricinus communis
GN=RCOM_0035800 PE=4 SV=1
Length = 652
Score = 573 bits (1475), Expect = 4e-161
Identities = 299/513 (58%), Positives = 375/513 (73%), Gaps = 11/513 (2%)
Frame = -3
Query: 1726 SSSKTKTADEVDGDPEVVLRAWLRSKFDEFVKLLLDVLVSQQSEDTLRDIVLGTLMEFVK 1547
SS K+K D D V+ + WLRSKFDE VK LL++L+S + ++TLRD+VL ++MEFVK
Sbjct: 115 SSKKSKKTTTDDDDAAVIYKTWLRSKFDELVKSLLNLLLSVELDETLRDVVLDSVMEFVK 174
Query: 1546 LLNAGRFHSSIYHRILNAIIHSAVDADIFLDILNSNYFKYIDVRYFTYISMEKFVKTL-E 1370
L N GRF+S+IYHR+L ++ SA D L++L S YF YID+ YFTYIS++K KT+ E
Sbjct: 175 LGNGGRFNSAIYHRLLYNLVQSATPVDFVLELLASKYFNYIDICYFTYISLDKLAKTMGE 234
Query: 1369 ASAVSADRTVNENSETENESKDSLEHSIRKIYQVLSRTPPPQKQAEKSGHEMWSGSDESS 1190
+ N + + + S++ SI KI+ +LS P + E S ++MWSG +
Sbjct: 235 NDSPDNKAKSAANGDDASHPRASMDLSIHKIHYILSCIPTVEDPKENSDNKMWSGLVVFN 294
Query: 1189 SEKPKDKKKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQT 1010
Q +LS +I+K+MKLKFTKAWISFLRLPLP++VYKEVL S+HQ
Sbjct: 295 L---YSSVLTLLCMQSIQVLSAASISKKMKLKFTKAWISFLRLPLPVNVYKEVLISLHQA 351
Query: 1009 VIPHLSNPAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVF 830
VIP++SNP MLCDFLT+SYDIGGVVSVMALSSLFILMT+HGLEYPNFYEKLYALL+PSVF
Sbjct: 352 VIPYISNPLMLCDFLTRSYDIGGVVSVMALSSLFILMTQHGLEYPNFYEKLYALLLPSVF 411
Query: 829 VAKHRARFLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHP 650
+AKHR++F QLLD+CLKS LLPAYLAA+FAK+LSRL+L+ PPSG +VI ALI+NLLRRHP
Sbjct: 412 MAKHRSKFFQLLDSCLKSPLLPAYLAAAFAKRLSRLALTAPPSGGVVIIALIHNLLRRHP 471
Query: 649 TINHLVHQE-----PVENANEANSEADEDNESSRPK--TNKKLGIDYFNNQETDLKKTGA 491
+IN LVH+E +N+ +A + N S + +K GID FNN+E K+ A
Sbjct: 472 SINCLVHREDGNESAADNSKAKGEDAGDANNSRNGSHASARKPGIDRFNNEECSPIKSSA 531
Query: 490 MKSSLWEIDTLRHHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIFGDEIRRR 311
++SSLWEIDTL HHYCPPVSRFV SLE DLT+R KTTE+ I DFSS SYATIF +E+RRR
Sbjct: 532 LRSSLWEIDTLSHHYCPPVSRFVLSLENDLTVRKKTTEVNINDFSSSSYATIFEEELRRR 591
Query: 310 VKQVPLAFYKAVPTSLFEDSDFPGWTFNIPQDE 212
VKQVPLAF+KA PTSLF +SDF GWTF Q +
Sbjct: 592 VKQVPLAFFKATPTSLFSESDFAGWTFKYEQSK 624
>tr|Q01K64|Q01K64_ORYSA H0307D04.2 protein OS=Oryza sativa GN=H0307D04.2 PE=4
SV=1
Length = 601
Score = 377 bits (968), Expect = 3e-102
Identities = 197/315 (62%), Positives = 243/315 (77%), Gaps = 5/315 (1%)
Frame = -3
Query: 1168 KKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSN 989
K +KN +D S T I K++K KF+KAWISFL+LPLPLDVYKEVLA+IH+ VIP +SN
Sbjct: 255 KGEKNSSED---CSATYINKKLKSKFSKAWISFLKLPLPLDVYKEVLATIHKNVIPSMSN 311
Query: 988 PAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRAR 809
PA+LCDFLT+SYDIGGV+SVMALS LFILMT+HGLEYP FY+KLYALL P+VF+AKHRA
Sbjct: 312 PAILCDFLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYDKLYALLTPAVFMAKHRAV 371
Query: 808 FLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
FLQLLDACLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP+IN LVH
Sbjct: 372 FLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPAGALIIIALIHNLLRRHPSINFLVH 431
Query: 628 QEPVENANEANSEADEDNE-SSRPKTNKKLGIDYFNNQETDLKKTGAMKSSLW-EIDTLR 455
E N +E +EA + + + P N++ + L + ++ + +
Sbjct: 432 WEIDANDSEIFNEASQRKKVGADPFNNEETDPAKSGAMRSSLWEIDTLRHHYSPAVSSSF 491
Query: 454 HHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIFGDEIRRRVKQVPLAFYKAV 275
+ RFV+SLE DLT+RAKTTEMKI DFSSGSYAT+F DE+RRR+KQVPLAFY+
Sbjct: 492 ILILLTIVRFVASLENDLTVRAKTTEMKITDFSSGSYATVFRDEVRRRIKQVPLAFYRTT 551
Query: 274 PTSLFEDSDFPGWTF 230
PT LF++SDFPGWTF
Sbjct: 552 PTCLFQESDFPGWTF 566
>tr|Q7XP58|Q7XP58_ORYSJ OSJNBa0013K16.9 protein OS=Oryza sativa subsp. japonica
GN=OSJNBa0013K16.9 PE=4 SV=2
Length = 601
Score = 377 bits (968), Expect = 3e-102
Identities = 197/315 (62%), Positives = 243/315 (77%), Gaps = 5/315 (1%)
Frame = -3
Query: 1168 KKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSN 989
K +KN +D S T I K++K KF+KAWISFL+LPLPLDVYKEVLA+IH+ VIP +SN
Sbjct: 255 KGEKNSSED---CSATYINKKLKSKFSKAWISFLKLPLPLDVYKEVLATIHKNVIPSMSN 311
Query: 988 PAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRAR 809
PA+LCDFLT+SYDIGGV+SVMALS LFILMT+HGLEYP FY+KLYALL P+VF+AKHRA
Sbjct: 312 PAILCDFLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYDKLYALLTPAVFMAKHRAV 371
Query: 808 FLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
FLQLLDACLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP+IN LVH
Sbjct: 372 FLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPAGALIIIALIHNLLRRHPSINFLVH 431
Query: 628 QEPVENANEANSEADEDNE-SSRPKTNKKLGIDYFNNQETDLKKTGAMKSSLW-EIDTLR 455
E N +E +EA + + + P N++ + L + ++ + +
Sbjct: 432 WEIDANDSEIFNEASQRKKVGADPFNNEETDPAKSGAMRSSLWEIDTLRHHYSPAVSSSF 491
Query: 454 HHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIFGDEIRRRVKQVPLAFYKAV 275
+ RFV+SLE DLT+RAKTTEMKI DFSSGSYAT+F DE+RRR+KQVPLAFY+
Sbjct: 492 IVILLTIVRFVASLENDLTVRAKTTEMKITDFSSGSYATVFRDEVRRRIKQVPLAFYRTT 551
Query: 274 PTSLFEDSDFPGWTF 230
PT LF++SDFPGWTF
Sbjct: 552 PTCLFQESDFPGWTF 566
>tr|C6TAQ8|C6TAQ8_SOYBN Putative uncharacterized protein OS=Glycine max PE=2
SV=1
Length = 253
Score = 360 bits (922), Expect = 6e-097
Identities = 177/247 (71%), Positives = 207/247 (83%), Gaps = 6/247 (2%)
Frame = -3
Query: 1105 MKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGVVSVM 926
MKLKFTKAWI++LRLPLP+DVYKEVL ++HQ VIPHLSNP MLCDFLT+SYD+GGVVSVM
Sbjct: 1 MKLKFTKAWIAYLRLPLPIDVYKEVLVNLHQAVIPHLSNPIMLCDFLTRSYDVGGVVSVM 60
Query: 925 ALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLAAS 746
ALSSLF+LMT++GLEYPNFYEKLYALLVPS+F+AKHRARF QLLD+CLKS LLPAYLAAS
Sbjct: 61 ALSSLFVLMTQYGLEYPNFYEKLYALLVPSIFMAKHRARFFQLLDSCLKSPLLPAYLAAS 120
Query: 745 FAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVENANEANSEADE----- 581
FAKKLSRL LSVPPSG+LVITALI+N+LRRHP+IN LVH+E + + + DE
Sbjct: 121 FAKKLSRLLLSVPPSGALVITALIHNILRRHPSINCLVHREDGVDEGKGDHRTDEGMATN 180
Query: 580 -DNESSRPKTNKKLGIDYFNNQETDLKKTGAMKSSLWEIDTLRHHYCPPVSRFVSSLETD 404
DN + ++K GID+FN+ ETD KK+ AM+SSLWEIDT+ HHYCPP SRF SL D
Sbjct: 181 SDNAKTVAMPSQKSGIDHFNSSETDPKKSDAMRSSLWEIDTILHHYCPPASRFALSLGND 240
Query: 403 LTIRAKT 383
LT+R T
Sbjct: 241 LTVRQNT 247
>tr|A9RUI9|A9RUI9_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_161062 PE=4 SV=1
Length = 671
Score = 326 bits (835), Expect = 7e-087
Identities = 191/486 (39%), Positives = 283/486 (58%), Gaps = 42/486 (8%)
Frame = -3
Query: 1969 NHTLKELKSLGSDLLSSRSHINNLPLLLSFI------SPSSPPQFVVESLLSLQSFFTPL 1808
N L K LG +LL SR+H+NN PLLL+ + S ++ + E+LLSLQ+FF PL
Sbjct: 4 NKQLAVTKKLGKELLLSRAHVNNAPLLLATLTSSLQDSTTANAAVLKEALLSLQAFFVPL 63
Query: 1807 LSQLPPSSPPPSSTKRPRSGEEEEEDDSSSKTKTADEVD-GDPEVVLRAWLRSKFDEFVK 1631
+ S S + R+ EE + +S+ ++ + + + + W+ ++ EFV
Sbjct: 64 V-----KSGDFSPAAQKRASEELQRVSGTSEADGGNQAEMAKADAIYKKWIWDRYREFVT 118
Query: 1630 LLLDVLVSQQSEDTLRDIVLGTLMEFVKLLNAGRFHSSIYHRILNAIIHSAVDADIFLDI 1451
LL + + ++ L T+ME V++ G F++ +Y R+ + + S L +
Sbjct: 119 TLLRFVARHNAIPEVQIAALHTIMELVRVEVYGEFNNHLYLRLCSTFVFSKAFNSGLLSV 178
Query: 1450 LNSNYFKYIDVRYFTYISMEKF-VKTLEASAVSADRTVNENSETENESK----------- 1307
S+YFKYIDV Y+TY +M+K VK L A + +E+ N ++
Sbjct: 179 FVSDYFKYIDVCYYTYSNMDKITVKRLSQKGSEAISSDDEDDNLRNFTRFANIVQIFEVY 238
Query: 1306 -----DSLEHSIRKIYQVLSRTPPPQKQAEKSGHEMWSGSDESSSEKPKDKKKKKNKDQD 1142
+ +Y +L P +++ E+WS S S +D K
Sbjct: 239 GAHTYGGVHSMASNVYDILINLP---TFSQEDCTEVWSFSAGS-----EDGGTISGKKVS 290
Query: 1141 SNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLT 962
+ T K+ + + +KAW++FL+LP P ++YK+VLA +H+ VIP LSNP +L DFLT
Sbjct: 291 GEVSQHYTNQKKQRTRVSKAWLAFLKLPFPYEIYKKVLAQLHKRVIPFLSNPVLLSDFLT 350
Query: 961 KSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACL 782
SY++GG++SVMAL+ LFILMT HGLEYP+FY KLYALL PS+FVAKHRARF +L D CL
Sbjct: 351 NSYNVGGLISVMALNGLFILMTSHGLEYPDFYNKLYALLEPSIFVAKHRARFFELTDTCL 410
Query: 781 KSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVENANE 602
KS+ +PAYLAA+FAKKL RL+LS PPSG+LV+ A+I+NLLRRHP+IN LVH+ A+
Sbjct: 411 KSTHIPAYLAAAFAKKLGRLALSAPPSGALVVIAMIHNLLRRHPSINQLVHR-----ASS 465
Query: 601 ANSEAD 584
A++ +D
Sbjct: 466 ASTSSD 471
>tr|B6SWX2|B6SWX2_MAIZE Nucleolar complex protein 4 OS=Zea mays PE=2 SV=1
Length = 593
Score = 283 bits (722), Expect = 9e-074
Identities = 138/193 (71%), Positives = 167/193 (86%)
Frame = -3
Query: 1150 DQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCD 971
+ DS+ + T I K++KLKFTKAW+SFL+LPLPLDVYKEVLASIHQ VIP +SNP++LCD
Sbjct: 260 ENDSSKDTSTYINKKLKLKFTKAWLSFLKLPLPLDVYKEVLASIHQNVIPSMSNPSILCD 319
Query: 970 FLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLD 791
FLT+SYDIGGV+SVMALS LFILMT+HGLEYP FYEKLYALL P+VF+AKHR+ FLQLLD
Sbjct: 320 FLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYEKLYALLTPAVFMAKHRSVFLQLLD 379
Query: 790 ACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVEN 611
CLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP++N LVH E E+
Sbjct: 380 TCLKSSYLPAYLAAAFAKRLSRLALSVPPAGALIIIALIHNLLRRHPSVNFLVHWEVDES 439
Query: 610 ANEANSEADEDNE 572
+ A EA + +
Sbjct: 440 DSNATREASQSKK 452
>tr|C4IY33|C4IY33_MAIZE Putative uncharacterized protein OS=Zea mays PE=2 SV=1
Length = 345
Score = 283 bits (722), Expect = 9e-074
Identities = 138/193 (71%), Positives = 167/193 (86%)
Frame = -3
Query: 1150 DQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCD 971
+ DS+ + T I K++KLKFTKAW+SFL+LPLPLDVYKEVLASIHQ VIP +SNP++LCD
Sbjct: 12 ENDSSKDTSTYINKKLKLKFTKAWLSFLKLPLPLDVYKEVLASIHQNVIPSMSNPSILCD 71
Query: 970 FLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLD 791
FLT+SYDIGGV+SVMALS LFILMT+HGLEYP FYEKLYALL P+VF+AKHR+ FLQLLD
Sbjct: 72 FLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYEKLYALLTPAVFMAKHRSVFLQLLD 131
Query: 790 ACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVEN 611
CLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP++N LVH E E+
Sbjct: 132 TCLKSSYLPAYLAAAFAKRLSRLALSVPPAGALIIIALIHNLLRRHPSVNFLVHWEVDES 191
Query: 610 ANEANSEADEDNE 572
+ A EA + +
Sbjct: 192 DSNATREASQSKK 204
>tr|B8ATD3|B8ATD3_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
indica GN=OsI_17139 PE=4 SV=1
Length = 590
Score = 280 bits (715), Expect = 6e-073
Identities = 141/196 (71%), Positives = 168/196 (85%), Gaps = 3/196 (1%)
Frame = -3
Query: 1168 KKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSN 989
K +KN +D S T I K++K KF+KAWISFL+LPLPLDVYKEVLA+IH+ VIP +SN
Sbjct: 255 KGEKNSSED---CSATYINKKLKSKFSKAWISFLKLPLPLDVYKEVLATIHKNVIPSMSN 311
Query: 988 PAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRAR 809
PA+LCDFLT+SYDIGGV+SVMALS LFILMT+HGLEYP FY+KLYALL P+VF+AKHRA
Sbjct: 312 PAILCDFLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYDKLYALLTPAVFMAKHRAV 371
Query: 808 FLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
FLQLLDACLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP+IN LVH
Sbjct: 372 FLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPAGALIIIALIHNLLRRHPSINFLVH 431
Query: 628 QEPVENANEANSEADE 581
E N +E +EA +
Sbjct: 432 WEIDANDSEIFNEASQ 447
Score = 182 bits (460), Expect = 2e-043
Identities = 103/214 (48%), Positives = 132/214 (61%), Gaps = 7/214 (3%)
Frame = -3
Query: 868 YEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLV 689
Y K Y L + A A+ + L + L +YL A A ++ + S
Sbjct: 348 YPKFYDKLYALLTPAVFMAKHRAVFLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPA 407
Query: 688 ITALIYNLLRRHPTINHLVHQEPVEN-ANEANSEADEDNESSRPKTNKKLGIDYFNNQET 512
+I L I++L+ + P N +A++ + KK+G D FNN+ET
Sbjct: 408 GALIIIAL------IHNLLRRHPSINFLVHWEIDANDSEIFNEASQRKKVGADPFNNEET 461
Query: 511 DLKKTGAMKSSLWEIDTLRHHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIF 332
D K+GAM+SSLWEIDTLRHHY P VSRFV+SLE DLT+RAKTTEMKI DFSSGSYAT+F
Sbjct: 462 DPAKSGAMRSSLWEIDTLRHHYSPAVSRFVASLENDLTVRAKTTEMKITDFSSGSYATVF 521
Query: 331 GDEIRRRVKQVPLAFYKAVPTSLFEDSDFPGWTF 230
DE+RRR+KQVPLAFY+ PT LF++SDFPGWTF
Sbjct: 522 RDEVRRRIKQVPLAFYRTTPTCLFQESDFPGWTF 555
>tr|B9FC62|B9FC62_ORYSJ Putative uncharacterized protein OS=Oryza sativa subsp.
japonica GN=OsJ_15930 PE=4 SV=1
Length = 590
Score = 280 bits (715), Expect = 6e-073
Identities = 141/196 (71%), Positives = 168/196 (85%), Gaps = 3/196 (1%)
Frame = -3
Query: 1168 KKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSN 989
K +KN +D S T I K++K KF+KAWISFL+LPLPLDVYKEVLA+IH+ VIP +SN
Sbjct: 255 KGEKNSSED---CSATYINKKLKSKFSKAWISFLKLPLPLDVYKEVLATIHKNVIPSMSN 311
Query: 988 PAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRAR 809
PA+LCDFLT+SYDIGGV+SVMALS LFILMT+HGLEYP FY+KLYALL P+VF+AKHRA
Sbjct: 312 PAILCDFLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYDKLYALLTPAVFMAKHRAV 371
Query: 808 FLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
FLQLLDACLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP+IN LVH
Sbjct: 372 FLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPAGALIIIALIHNLLRRHPSINFLVH 431
Query: 628 QEPVENANEANSEADE 581
E N +E +EA +
Sbjct: 432 WEIDANDSEIFNEASQ 447
Score = 182 bits (460), Expect = 2e-043
Identities = 103/214 (48%), Positives = 132/214 (61%), Gaps = 7/214 (3%)
Frame = -3
Query: 868 YEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLV 689
Y K Y L + A A+ + L + L +YL A A ++ + S
Sbjct: 348 YPKFYDKLYALLTPAVFMAKHRAVFLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPA 407
Query: 688 ITALIYNLLRRHPTINHLVHQEPVEN-ANEANSEADEDNESSRPKTNKKLGIDYFNNQET 512
+I L I++L+ + P N +A++ + KK+G D FNN+ET
Sbjct: 408 GALIIIAL------IHNLLRRHPSINFLVHWEIDANDSEIFNEASQRKKVGADPFNNEET 461
Query: 511 DLKKTGAMKSSLWEIDTLRHHYCPPVSRFVSSLETDLTIRAKTTEMKIEDFSSGSYATIF 332
D K+GAM+SSLWEIDTLRHHY P VSRFV+SLE DLT+RAKTTEMKI DFSSGSYAT+F
Sbjct: 462 DPAKSGAMRSSLWEIDTLRHHYSPAVSRFVASLENDLTVRAKTTEMKITDFSSGSYATVF 521
Query: 331 GDEIRRRVKQVPLAFYKAVPTSLFEDSDFPGWTF 230
DE+RRR+KQVPLAFY+ PT LF++SDFPGWTF
Sbjct: 522 RDEVRRRIKQVPLAFYRTTPTCLFQESDFPGWTF 555
>tr|Q0JAP6|Q0JAP6_ORYSJ Os04g0585300 protein OS=Oryza sativa subsp. japonica
GN=Os04g0585300 PE=4 SV=1
Length = 590
Score = 280 bits (715), Expect = 6e-073
Identities = 141/196 (71%), Positives = 168/196 (85%), Gaps = 3/196 (1%)
Frame = -3
Query: 1168 KKKKNKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSN 989
K +KN +D S T I K++K KF+KAWISFL+LPLPLDVYKEVLA+IH+ VIP +SN
Sbjct: 255 KGEKNSSED---CSATYINKKLKSKFSKAWISFLKLPLPLDVYKEVLATIHKNVIPSMSN 311
Query: 988 PAMLCDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRAR 809
PA+LCDFLT+SYDIGGV+SVMALS LFILMT+HGLEYP FY+KLYALL P+VF+AKHRA
Sbjct: 312 PAILCDFLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYDKLYALLTPAVFMAKHRAV 371
Query: 808 FLQLLDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
FLQLLDACLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP+IN LVH
Sbjct: 372 FLQLLDACLKSSYLPAYLAAAFAKRLSRLTLSVPPAGALIIIALIHNLLRRHPSINFLVH 431
Query: 628 QEPVENANEANSEADE 581
E N +E +EA +
Sbjct: 432 WEIDANDSEIFNEASQ 447
>tr|C5YEL7|C5YEL7_SORBI Putative uncharacterized protein Sb06g026607 (Fragment)
OS=Sorghum bicolor GN=Sb06g026607 PE=4 SV=1
Length = 438
Score = 275 bits (702), Expect = 2e-071
Identities = 132/174 (75%), Positives = 159/174 (91%)
Frame = -3
Query: 1150 DQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCD 971
++DS+ + T I K+++LKFTKAW+SFL+LPLPLDVYKEVLASIHQ VIP +SNP++LCD
Sbjct: 262 EKDSSKDTSTHIKKKLQLKFTKAWLSFLKLPLPLDVYKEVLASIHQNVIPSMSNPSILCD 321
Query: 970 FLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLD 791
FLT+SYDIGGV+SVMALS LFILMT+HGLEYP FYEKLYALL P+VF+AKHR+ FLQLLD
Sbjct: 322 FLTRSYDIGGVISVMALSGLFILMTQHGLEYPKFYEKLYALLTPAVFMAKHRSVFLQLLD 381
Query: 790 ACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVH 629
CLKSS LPAYLAA+FAK+LSRL+LSVPP+G+L+I ALI+NLLRRHP++N LVH
Sbjct: 382 TCLKSSYLPAYLAAAFAKRLSRLALSVPPAGALIIIALIHNLLRRHPSVNFLVH 435
>tr|C1N5W2|C1N5W2_9CHLO Predicted protein OS=Micromonas pusilla CCMP1545
GN=MICPUCDRAFT_53064 PE=4 SV=1
Length = 635
Score = 188 bits (476), Expect = 3e-045
Identities = 90/171 (52%), Positives = 117/171 (68%)
Frame = -3
Query: 1111 KRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGVVS 932
KR + F AW++FLR P+P D+Y++ L S+H VIPHL NP +L DF T S D GG+
Sbjct: 357 KRHRRLFADAWLAFLRTPMPPDIYRKTLTSLHDDVIPHLPNPQLLSDFCTHSIDRGGLDG 416
Query: 931 VMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLA 752
++AL+ +F+LMT+HGLEYP FY KLY LL P F A+ R F +LLD LKSS LP YLA
Sbjct: 417 MLALNGIFVLMTKHGLEYPAFYAKLYGLLTPEAFHARGRGGFFELLDVFLKSSALPGYLA 476
Query: 751 ASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPVENANEA 599
A+F K+L+RL+L PP+G++ A +NLLRRHP LVH+ P E A
Sbjct: 477 AAFIKRLARLALRAPPAGAMTCVAFAHNLLRRHPGCAVLVHRGPTEERASA 527
>tr|Q00UD0|Q00UD0_OSTTA Predicted nucleolar protein involved in ribosome
biogenesis (ISS) OS=Ostreococcus tauri GN=Ot16g01200 PE=4 SV=1
Length = 621
Score = 188 bits (476), Expect = 3e-045
Identities = 104/262 (39%), Positives = 154/262 (58%), Gaps = 7/262 (2%)
Frame = -3
Query: 1336 ENSETENESKDSLEHSIRKIYQVLSRTPPPQKQAEKSGHEMWSGSDESSSEKPKDKKKKK 1157
E+ ++ N++ + + R + + L +++AEK G W D + KK+
Sbjct: 276 EDHQSGNDASNPRDDFARMLAK-LRAGQDAEEEAEKRG--PWC-EDAEGGAVDLESAKKR 331
Query: 1156 NKDQDSNLLSPTTIAKRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAML 977
K S S T R + F++AW++ LR P D+Y+++L +H V+PH+ NP +L
Sbjct: 332 RKISSSVGASKWTEGVRHRRAFSEAWLALLRAEFPEDIYRKILMRLHVDVMPHMVNPQLL 391
Query: 976 CDFLTKSYDIGGVVSVMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQL 797
DF S D+GG+ ++AL+ LFILMT+HGLEYP FY +LY LL S F A HR F L
Sbjct: 392 SDFCVDSIDVGGLTGMLALNGLFILMTQHGLEYPTFYNRLYQLLDGSCFHANHRRGFFSL 451
Query: 796 LDACLKSSLLPAYLAASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQEPV 617
+D LKS LPAYL ASF K+ +RLSLS PP+G+++ A I+NL+RRH + LVH+
Sbjct: 452 MDVFLKSPALPAYLVASFVKRFARLSLSAPPAGAMLCVAFIHNLIRRHKSCAVLVHR--- 508
Query: 616 ENANEANSEADEDNESSRPKTN 551
ENA +++ + +E KTN
Sbjct: 509 ENAATVDADPFDADEQDPAKTN 530
>tr|D2HLG5|D2HLG5_AILME Putative uncharacterized protein (Fragment)
OS=Ailuropoda melanoleuca GN=PANDA_012335 PE=4 SV=1
Length = 479
Score = 185 bits (468), Expect = 2e-044
Identities = 93/185 (50%), Positives = 128/185 (69%), Gaps = 3/185 (1%)
Frame = -3
Query: 1111 KRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGVVS 932
K + F W+ FL+ LPL +YK+VL +H +++PHL+ P+++ DFLT++YD+GG +S
Sbjct: 209 KEHRKAFQLMWLGFLKHKLPLSLYKKVLVIMHDSILPHLAQPSLMIDFLTRAYDVGGAIS 268
Query: 931 VMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLA 752
++AL+ LFIL+ +H LEYP+FY KLY LL PSVF K+RARF L D L SS LPAYL
Sbjct: 269 LLALNGLFILIHQHNLEYPDFYRKLYGLLDPSVFHVKYRARFFHLADLFLSSSHLPAYLV 328
Query: 751 ASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQE--PVENANEANSEADED 578
A+FAK+LSRL+L+ PP L++ I NLLRRHP LVH+ P +A+ + E +ED
Sbjct: 329 AAFAKRLSRLALTAPPEALLMVLPFICNLLRRHPACRVLVHRPLGPELDADPYDPE-EED 387
Query: 577 NESSR 563
SR
Sbjct: 388 PAKSR 392
>tr|Q4T6B1|Q4T6B1_TETNG Chromosome undetermined SCAF8839, whole genome shotgun
sequence. (Fragment) OS=Tetraodon nigroviridis GN=GSTENG00006400001
PE=4 SV=1
Length = 515
Score = 184 bits (465), Expect = 5e-044
Identities = 83/162 (51%), Positives = 119/162 (73%)
Frame = -3
Query: 1111 KRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGVVS 932
K + F K W++FL+ LP ++YK+VL +H +V+PH+S P ++ DFLT +YD+GG +S
Sbjct: 260 KEHRRLFQKMWLAFLKYRLPSNLYKKVLVILHDSVLPHMSEPTLMMDFLTAAYDVGGAIS 319
Query: 931 VMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLA 752
++AL+ LF+L+ EH L+YP+FY KLYALL P++F K+RARF L D L SS LPAYL
Sbjct: 320 LLALNGLFVLIHEHNLDYPDFYRKLYALLEPTIFHVKYRARFFHLADLFLSSSHLPAYLV 379
Query: 751 ASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQ 626
A+F K+ +RL+L+ PP+G LV+ I NL+RRHP+ L+HQ
Sbjct: 380 AAFVKRFARLALTAPPAGLLVLLPFITNLIRRHPSCRVLLHQ 421
>tr|Q3UKH4|Q3UKH4_MOUSE Putative uncharacterized protein OS=Mus musculus
GN=Noc4l PE=2 SV=1
Length = 516
Score = 182 bits (461), Expect = 2e-043
Identities = 84/162 (51%), Positives = 118/162 (72%)
Frame = -3
Query: 1111 KRMKLKFTKAWISFLRLPLPLDVYKEVLASIHQTVIPHLSNPAMLCDFLTKSYDIGGVVS 932
K K F + W+ FL+ LPL +YK+VL ++H +++PHL+ P ++ DFLT + D+GG +S
Sbjct: 246 KEHKKAFQEMWLGFLKHKLPLSLYKKVLVAMHDSILPHLAQPTLMIDFLTSACDVGGAIS 305
Query: 931 VMALSSLFILMTEHGLEYPNFYEKLYALLVPSVFVAKHRARFLQLLDACLKSSLLPAYLA 752
++AL+ LFIL+ +H LEYP+FY+KLY LL PS+F K+RARF L D L SS LPAYL
Sbjct: 306 LLALNGLFILIHKHNLEYPDFYQKLYGLLDPSIFRVKYRARFFHLADLFLSSSHLPAYLV 365
Query: 751 ASFAKKLSRLSLSVPPSGSLVITALIYNLLRRHPTINHLVHQ 626
A+FAK+L+RL+L+ PP L++ LI NLLRRHP +VH+
Sbjct: 366 AAFAKRLARLALTAPPEALLMVLPLICNLLRRHPACRVMVHR 407
Database: UniProt/TrEMBL
Posted date: Sat Aug 07 14:51:12 2010
Number of letters in database: 3,661,877,547
Number of sequences in database: 11,397,958
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 544,157,141,797
Number of Sequences: 11397958
Number of Extensions: 544157141797
Number of Successful Extensions: 231549180
Number of sequences better than 0.0: 0
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