BLASTX 7.6.2
Query= UN08101 /QuerySize=1627
(1626 letters)
Database: TAIR9 protein;
33,410 sequences; 13,468,323 total letters
Score E
Sequences producing significant alignments: (bits) Value
TAIR9_protein||AT4G36690.1 | Symbols: ATU2AF65A | ATU2AF65A; RNA... 386 4e-107
TAIR9_protein||AT4G36690.3 | Symbols: ATU2AF65A | ATU2AF65A; RNA... 386 4e-107
TAIR9_protein||AT4G36690.2 | Symbols: ATU2AF65A | ATU2AF65A; RNA... 386 4e-107
TAIR9_protein||AT1G60900.1 | Symbols: | U2 snRNP auxiliary fact... 334 1e-091
TAIR9_protein||AT1G60830.1 | Symbols: | U2 snRNP auxiliary fact... 150 3e-036
TAIR9_protein||AT1G71800.1 | Symbols: | cleavage stimulation fa... 60 5e-009
TAIR9_protein||AT3G25840.1 | Symbols: | protein kinase family p... 58 1e-008
TAIR9_protein||AT3G25840.2 | Symbols: | protein kinase family p... 58 1e-008
TAIR9_protein||AT3G03340.1 | Symbols: UNE6 | UNE6 (unfertilized ... 58 2e-008
TAIR9_protein||AT5G61030.1 | Symbols: GR-RBP3 | GR-RBP3 (glycine... 53 6e-007
TAIR9_protein||AT4G00830.1 | Symbols: | RNA recognition motif (... 52 1e-006
>TAIR9_protein||AT4G36690.1 | Symbols: ATU2AF65A | ATU2AF65A; RNA binding /
nucleic acid binding / nucleotide binding | chr4:17294139-17297609
REVERSE
Length = 574
Score = 386 bits (989), Expect = 4e-107
Identities = 218/285 (76%), Positives = 237/285 (83%), Gaps = 21/285 (7%)
Frame = +2
Query: 5 DRDGERIKRHDDREDDRRSRDRDRHHR-SRHRDRS---GERRDR-VRGDDDYRRSSRDRD 169
DRDGE+ K +R D+ R+RHHR SRHRD S GERR+R R DDDYRR SRDRD
Sbjct: 76 DRDGEKSK---ERSRDKDRDHRERHHRSSRHRDHSRERGERRERGGRDDDDYRR-SRDRD 131
Query: 170 FDRRRDSDRDRGGDR-RRRRSRSRGRSER--RSRSPSKTK-RVSGFDMAPPAAGMLDAGA 337
DRRRD DRGG R RR RSRS+ RSER RSRSPSK+K RVSGFDMAPPA+ ML AGA
Sbjct: 132 HDRRRD---DRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSGFDMAPPASAMLAAGA 188
Query: 338 ALTGQVPAPIPNLLPGNG----MFPLQPGQQFGGVPMMPIQAMTQQATRHARRVYVGGLS 505
A+TGQVP P P LPG G MFPL GQ FGG+ MMPIQAMTQQATRHARRVYVGGLS
Sbjct: 189 AVTGQVP-PAPPTLPGAGMFPNMFPLPTGQSFGGLSMMPIQAMTQQATRHARRVYVGGLS 247
Query: 506 PSANEQSVATFFSQAMAAIGGNSAGPGDAVVNVYINYEKKFAFVEMRSVQEASNAMTLDG 685
P+ANEQSVATFFSQ MAA+GGN+AGPGDAVVNVYIN+EKKFAFVEMRSV+EASNAM+LDG
Sbjct: 248 PTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDG 307
Query: 686 IIFEGAPVKVRRPSDYNPALAATLGPSQPIPNLNLAAVGLTPGGA 820
IIFEGAPVKVRRPSDYNP+LAATLGPSQP P+LNLAAVGLTPG +
Sbjct: 308 IIFEGAPVKVRRPSDYNPSLAATLGPSQPSPHLNLAAVGLTPGAS 352
Score = 359 bits (919), Expect = 5e-099
Identities = 177/219 (80%), Positives = 192/219 (87%), Gaps = 2/219 (0%)
Frame = +3
Query: 825 LEGPDRLFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATD 1004
LEGPDR+FVGGLPYY ESQ+RELLES G LKGFDLV+DRETGNSKGYAFC YQD + TD
Sbjct: 355 LEGPDRIFVGGLPYYFTESQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVTD 414
Query: 1005 IACAALNGITMGDKTLCVRRANQGTAQTKPEQENVLLHAQQQVAFQKIMLQQGAAAAATK 1184
IACAALNGI MGDKTL VRRANQGT KPEQENVLLHAQQQ+AFQ++MLQ G A AT
Sbjct: 415 IACAALNGIKMGDKTLTVRRANQGTMLQKPEQENVLLHAQQQIAFQRVMLQPG--AVATT 472
Query: 1185 VVCLTQVVTEDELRNDEEYEDIVVDMRDEGGKLGALTKVVIPRPSSSGEPVQGVGKVFLE 1364
VVCLTQVVTEDELR+DEEY DI+ DMR EGGK GALT VVIPRPS +GEPV G+GKVFL+
Sbjct: 473 VVCLTQVVTEDELRDDEEYGDIMEDMRQEGGKFGALTNVVIPRPSPNGEPVAGLGKVFLK 532
Query: 1365 YAETESSNRARNGMNGRKFGGNEVVAVFYPEDKFEQGDY 1481
YA+T+ S RAR GMNGRKFGGNEVVAV+YPEDKFEQGDY
Sbjct: 533 YADTDGSTRARFGMNGRKFGGNEVVAVYYPEDKFEQGDY 571
>TAIR9_protein||AT4G36690.3 | Symbols: ATU2AF65A | ATU2AF65A; RNA binding /
nucleic acid binding / nucleotide binding | chr4:17294422-17297609
REVERSE
Length = 566
Score = 386 bits (989), Expect = 4e-107
Identities = 218/285 (76%), Positives = 237/285 (83%), Gaps = 21/285 (7%)
Frame = +2
Query: 5 DRDGERIKRHDDREDDRRSRDRDRHHR-SRHRDRS---GERRDR-VRGDDDYRRSSRDRD 169
DRDGE+ K +R D+ R+RHHR SRHRD S GERR+R R DDDYRR SRDRD
Sbjct: 76 DRDGEKSK---ERSRDKDRDHRERHHRSSRHRDHSRERGERRERGGRDDDDYRR-SRDRD 131
Query: 170 FDRRRDSDRDRGGDR-RRRRSRSRGRSER--RSRSPSKTK-RVSGFDMAPPAAGMLDAGA 337
DRRRD DRGG R RR RSRS+ RSER RSRSPSK+K RVSGFDMAPPA+ ML AGA
Sbjct: 132 HDRRRD---DRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSGFDMAPPASAMLAAGA 188
Query: 338 ALTGQVPAPIPNLLPGNG----MFPLQPGQQFGGVPMMPIQAMTQQATRHARRVYVGGLS 505
A+TGQVP P P LPG G MFPL GQ FGG+ MMPIQAMTQQATRHARRVYVGGLS
Sbjct: 189 AVTGQVP-PAPPTLPGAGMFPNMFPLPTGQSFGGLSMMPIQAMTQQATRHARRVYVGGLS 247
Query: 506 PSANEQSVATFFSQAMAAIGGNSAGPGDAVVNVYINYEKKFAFVEMRSVQEASNAMTLDG 685
P+ANEQSVATFFSQ MAA+GGN+AGPGDAVVNVYIN+EKKFAFVEMRSV+EASNAM+LDG
Sbjct: 248 PTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDG 307
Query: 686 IIFEGAPVKVRRPSDYNPALAATLGPSQPIPNLNLAAVGLTPGGA 820
IIFEGAPVKVRRPSDYNP+LAATLGPSQP P+LNLAAVGLTPG +
Sbjct: 308 IIFEGAPVKVRRPSDYNPSLAATLGPSQPSPHLNLAAVGLTPGAS 352
Score = 247 bits (630), Expect = 2e-065
Identities = 123/152 (80%), Positives = 132/152 (86%), Gaps = 2/152 (1%)
Frame = +3
Query: 825 LEGPDRLFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATD 1004
LEGPDR+FVGGLPYY ESQ+RELLES G LKGFDLV+DRETGNSKGYAFC YQD + TD
Sbjct: 355 LEGPDRIFVGGLPYYFTESQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVTD 414
Query: 1005 IACAALNGITMGDKTLCVRRANQGTAQTKPEQENVLLHAQQQVAFQKIMLQQGAAAAATK 1184
IACAALNGI MGDKTL VRRANQGT KPEQENVLLHAQQQ+AFQ++MLQ G A AT
Sbjct: 415 IACAALNGIKMGDKTLTVRRANQGTMLQKPEQENVLLHAQQQIAFQRVMLQPG--AVATT 472
Query: 1185 VVCLTQVVTEDELRNDEEYEDIVVDMRDEGGK 1280
VVCLTQVVTEDELR+DEEY DI+ DMR EGGK
Sbjct: 473 VVCLTQVVTEDELRDDEEYGDIMEDMRQEGGK 504
>TAIR9_protein||AT4G36690.2 | Symbols: ATU2AF65A | ATU2AF65A; RNA binding /
nucleic acid binding / nucleotide binding | chr4:17294591-17297609
REVERSE
Length = 543
Score = 386 bits (989), Expect = 4e-107
Identities = 218/285 (76%), Positives = 237/285 (83%), Gaps = 21/285 (7%)
Frame = +2
Query: 5 DRDGERIKRHDDREDDRRSRDRDRHHR-SRHRDRS---GERRDR-VRGDDDYRRSSRDRD 169
DRDGE+ K +R D+ R+RHHR SRHRD S GERR+R R DDDYRR SRDRD
Sbjct: 76 DRDGEKSK---ERSRDKDRDHRERHHRSSRHRDHSRERGERRERGGRDDDDYRR-SRDRD 131
Query: 170 FDRRRDSDRDRGGDR-RRRRSRSRGRSER--RSRSPSKTK-RVSGFDMAPPAAGMLDAGA 337
DRRRD DRGG R RR RSRS+ RSER RSRSPSK+K RVSGFDMAPPA+ ML AGA
Sbjct: 132 HDRRRD---DRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSGFDMAPPASAMLAAGA 188
Query: 338 ALTGQVPAPIPNLLPGNG----MFPLQPGQQFGGVPMMPIQAMTQQATRHARRVYVGGLS 505
A+TGQVP P P LPG G MFPL GQ FGG+ MMPIQAMTQQATRHARRVYVGGLS
Sbjct: 189 AVTGQVP-PAPPTLPGAGMFPNMFPLPTGQSFGGLSMMPIQAMTQQATRHARRVYVGGLS 247
Query: 506 PSANEQSVATFFSQAMAAIGGNSAGPGDAVVNVYINYEKKFAFVEMRSVQEASNAMTLDG 685
P+ANEQSVATFFSQ MAA+GGN+AGPGDAVVNVYIN+EKKFAFVEMRSV+EASNAM+LDG
Sbjct: 248 PTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDG 307
Query: 686 IIFEGAPVKVRRPSDYNPALAATLGPSQPIPNLNLAAVGLTPGGA 820
IIFEGAPVKVRRPSDYNP+LAATLGPSQP P+LNLAAVGLTPG +
Sbjct: 308 IIFEGAPVKVRRPSDYNPSLAATLGPSQPSPHLNLAAVGLTPGAS 352
Score = 250 bits (636), Expect = 3e-066
Identities = 124/154 (80%), Positives = 133/154 (86%), Gaps = 2/154 (1%)
Frame = +3
Query: 825 LEGPDRLFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATD 1004
LEGPDR+FVGGLPYY ESQ+RELLES G LKGFDLV+DRETGNSKGYAFC YQD + TD
Sbjct: 355 LEGPDRIFVGGLPYYFTESQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVTD 414
Query: 1005 IACAALNGITMGDKTLCVRRANQGTAQTKPEQENVLLHAQQQVAFQKIMLQQGAAAAATK 1184
IACAALNGI MGDKTL VRRANQGT KPEQENVLLHAQQQ+AFQ++MLQ G A AT
Sbjct: 415 IACAALNGIKMGDKTLTVRRANQGTMLQKPEQENVLLHAQQQIAFQRVMLQPG--AVATT 472
Query: 1185 VVCLTQVVTEDELRNDEEYEDIVVDMRDEGGKLG 1286
VVCLTQVVTEDELR+DEEY DI+ DMR EGGK G
Sbjct: 473 VVCLTQVVTEDELRDDEEYGDIMEDMRQEGGKFG 506
>TAIR9_protein||AT1G60900.1 | Symbols: | U2 snRNP auxiliary factor large
subunit, putative | chr1:22424008-22427806 FORWARD
Length = 590
Score = 334 bits (855), Expect = 1e-091
Identities = 188/275 (68%), Positives = 209/275 (76%), Gaps = 26/275 (9%)
Frame = +2
Query: 56 RSRDRDRHHRSRHRDRSGERRD-RVRGDDDYRRSSRDRDFDRRRDSDRDRG-GDRRRRRS 229
+ R RDRHHR RHRDRS ER + R DDD+ R SRDR DRRR DRDR RRR RS
Sbjct: 96 KDRQRDRHHRDRHRDRSRERSEKRDDLDDDHHRRSRDR--DRRRSRDRDREVRHRRRSRS 153
Query: 230 RSRGRSERRSRSP----------------SKTKRVSGFDMAPPAAGMLDAGA-ALTGQVP 358
RSR RSERRSRS SK+KR SGFDMAPP ML A A A GQVP
Sbjct: 154 RSRSRSERRSRSEHRHKSEHRSRSRSRSRSKSKRRSGFDMAPP--DMLAATAVAAAGQVP 211
Query: 359 A-PIPNLLPG--NGMFPLQPGQQFGGVPMMPIQAMTQQATRHARRVYVGGLSPSANEQSV 529
+ P +PG + MFP+ PGQQ G +P++P+QAMTQQATRHARRVYVGGL P+ANEQSV
Sbjct: 212 SVPTTATIPGMFSNMFPMVPGQQLGALPVLPVQAMTQQATRHARRVYVGGLPPTANEQSV 271
Query: 530 ATFFSQAMAAIGGNSAGPGDAVVNVYINYEKKFAFVEMRSVQEASNAMTLDGIIFEGAPV 709
+TFFSQ M+AIGGN+AGPGDAVVNVYIN+EKKFAFVEMRSV+EASNAM LDGII EG PV
Sbjct: 272 STFFSQVMSAIGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMALDGIILEGVPV 331
Query: 710 KVRRPSDYNPALAATLGPSQPIPNLNLAAVGLTPG 814
KVRRP+DYNP+LAATLGPSQP PNLNL AVGL+ G
Sbjct: 332 KVRRPTDYNPSLAATLGPSQPNPNLNLGAVGLSSG 366
Score = 325 bits (831), Expect = 8e-089
Identities = 157/219 (71%), Positives = 181/219 (82%), Gaps = 2/219 (0%)
Frame = +3
Query: 825 LEGPDRLFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATD 1004
LEGPDR+FVGGLPYY E QIRELLES G L+GF+LV+DRETGNSKGYAFC YQDP+ TD
Sbjct: 371 LEGPDRIFVGGLPYYFTEVQIRELLESFGPLRGFNLVKDRETGNSKGYAFCVYQDPSVTD 430
Query: 1005 IACAALNGITMGDKTLCVRRANQGTAQTKPEQENVLLHAQQQVAFQKIMLQQGAAAAATK 1184
IACAALNGI MGDKTL VRRA QG Q KPEQE VLL+AQQQ+A Q++M Q G TK
Sbjct: 431 IACAALNGIKMGDKTLTVRRAIQGAIQPKPEQEEVLLYAQQQIALQRLMFQPG--GTPTK 488
Query: 1185 VVCLTQVVTEDELRNDEEYEDIVVDMRDEGGKLGALTKVVIPRPSSSGEPVQGVGKVFLE 1364
+VCLTQVVT D+LR+DEEY +I+ DMR EGGK G L VVIPRP+ +P GVGKVFLE
Sbjct: 489 IVCLTQVVTADDLRDDEEYAEIMEDMRQEGGKFGNLVNVVIPRPNPDHDPTPGVGKVFLE 548
Query: 1365 YAETESSNRARNGMNGRKFGGNEVVAVFYPEDKFEQGDY 1481
YA+ + S++AR+GMNGRKFGGN+VVAV+YPEDK+ QGDY
Sbjct: 549 YADVDGSSKARSGMNGRKFGGNQVVAVYYPEDKYAQGDY 587
>TAIR9_protein||AT1G60830.1 | Symbols: | U2 snRNP auxiliary factor large
subunit, putative | chr1:22395058-22395603 REVERSE
Length = 112
Score = 150 bits (378), Expect = 3e-036
Identities = 69/101 (68%), Positives = 83/101 (82%)
Frame = +3
Query: 1179 TKVVCLTQVVTEDELRNDEEYEDIVVDMRDEGGKLGALTKVVIPRPSSSGEPVQGVGKVF 1358
TK+VCLTQVVT D+LR+D EY DI+ DM EGGK G L VVIPRP+ +P GVGKVF
Sbjct: 9 TKIVCLTQVVTADDLRDDAEYADIMEDMSQEGGKFGNLVNVVIPRPNPDHDPTPGVGKVF 68
Query: 1359 LEYAETESSNRARNGMNGRKFGGNEVVAVFYPEDKFEQGDY 1481
LEYA+ + S++AR+GMNGRKFGGN+VVAV+YPEDK+ QGDY
Sbjct: 69 LEYADVDGSSKARSGMNGRKFGGNQVVAVYYPEDKYAQGDY 109
>TAIR9_protein||AT1G71800.1 | Symbols: | cleavage stimulation factor, putative
| chr1:26999606-27001850 FORWARD
Length = 462
Score = 60 bits (143), Expect = 5e-009
Identities = 33/89 (37%), Positives = 48/89 (53%), Gaps = 2/89 (2%)
Frame = +3
Query: 843 LFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATDIACAAL 1022
+FVG +PY E Q+RE+ +G + F LV DRETG KGY FC Y+D A L
Sbjct: 11 VFVGNIPYDATEEQLREICGEVGPVVSFRLVTDRETGKPKGYGFCEYKDEETALSARRNL 70
Query: 1023 NGITMGDKTLCVRRA--NQGTAQTKPEQE 1103
+ + L V A ++GT +T+ + +
Sbjct: 71 QSYEINGRQLRVDFAENDKGTDKTRDQSQ 99
>TAIR9_protein||AT3G25840.1 | Symbols: | protein kinase family protein |
chr3:9452993-9457446 REVERSE
Length = 936
Score = 58 bits (139), Expect = 1e-008
Identities = 32/85 (37%), Positives = 43/85 (50%)
Frame = +2
Query: 2 RDRDGERIKRHDDREDDRRSRDRDRHHRSRHRDRSGERRDRVRGDDDYRRSSRDRDFDRR 181
RDRD E KR D D RR R+R++ + ++R R D RR R+ D+ R
Sbjct: 270 RDRDSEGSKRRDKDSDRRREREREKRREIESDRERRKEKERERSIDRDRRKEREGDYLRD 329
Query: 182 RDSDRDRGGDRRRRRSRSRGRSERR 256
R ++R R DR R SR R R + R
Sbjct: 330 RANERGRSRDRTRYNSRERKREKER 354
>TAIR9_protein||AT3G25840.2 | Symbols: | protein kinase family protein |
chr3:9452993-9456322 REVERSE
Length = 674
Score = 58 bits (139), Expect = 1e-008
Identities = 32/85 (37%), Positives = 43/85 (50%)
Frame = +2
Query: 2 RDRDGERIKRHDDREDDRRSRDRDRHHRSRHRDRSGERRDRVRGDDDYRRSSRDRDFDRR 181
RDRD E KR D D RR R+R++ + ++R R D RR R+ D+ R
Sbjct: 8 RDRDSEGSKRRDKDSDRRREREREKRREIESDRERRKEKERERSIDRDRRKEREGDYLRD 67
Query: 182 RDSDRDRGGDRRRRRSRSRGRSERR 256
R ++R R DR R SR R R + R
Sbjct: 68 RANERGRSRDRTRYNSRERKREKER 92
>TAIR9_protein||AT3G03340.1 | Symbols: UNE6 | UNE6 (unfertilized embryo sac 6) |
chr3:786439-789762 FORWARD
Length = 403
Score = 58 bits (138), Expect = 2e-008
Identities = 35/89 (39%), Positives = 48/89 (53%), Gaps = 6/89 (6%)
Frame = +2
Query: 35 DDREDDRRSRDRDRHHRSRH--RDRSGERRDRVRGDDDYRRSSRDR----DFDRRRDSDR 196
D++ + R+ R ++R+ + R +DR E+ DY R SRDR D DR +D D
Sbjct: 310 DEKANIRKERSKERNSKERESSKDREKEQETSREHRRDYDRRSRDRDRHHDRDREQDRDY 369
Query: 197 DRGGDRRRRRSRSRGRSERRSRSPSKTKR 283
DR R RRRSRSR RS R R + +R
Sbjct: 370 DRSHSRSRRRSRSRSRSRDRPRDYDRHRR 398
>TAIR9_protein||AT5G61030.1 | Symbols: GR-RBP3 | GR-RBP3 (glycine-rich
RNA-binding protein 3); ATP binding / RNA binding |
chr5:24560870-24562152 FORWARD
Length = 310
Score = 53 bits (125), Expect = 6e-007
Identities = 26/81 (32%), Positives = 42/81 (51%)
Frame = +3
Query: 840 RLFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATDIACAA 1019
+LF+GG+ Y + E +RE G + ++ DRETG S+G+ F + A A A
Sbjct: 41 KLFIGGMAYSMDEDSLREAFTKYGEVVDTRVILDRETGRSRGFGFVTFTSSEAASSAIQA 100
Query: 1020 LNGITMGDKTLCVRRANQGTA 1082
L+G + + + V AN T+
Sbjct: 101 LDGRDLHGRVVKVNYANDRTS 121
>TAIR9_protein||AT4G00830.1 | Symbols: | RNA recognition motif (RRM)-containing
protein | chr4:352782-354965 FORWARD
Length = 496
Score = 52 bits (123), Expect = 1e-006
Identities = 25/70 (35%), Positives = 41/70 (58%)
Frame = +3
Query: 843 LFVGGLPYYIPESQIRELLESLGALKGFDLVRDRETGNSKGYAFCAYQDPAATDIACAAL 1022
+F+GGLP + E +R+L E +G + L++DR++G+SKGYAF A++ A L
Sbjct: 118 VFIGGLPRDVGEEDLRDLCEEIGEIFEVRLMKDRDSGDSKGYAFVAFKTKDVAQKAIEEL 177
Query: 1023 NGITMGDKTL 1052
+ KT+
Sbjct: 178 HSKEFKGKTI 187
Database: TAIR9 protein
Posted date: Wed Jul 08 15:16:08 2009
Number of letters in database: 13,468,323
Number of sequences in database: 33,410
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,671,488,970
Number of Sequences: 33410
Number of Extensions: 4671488970
Number of Successful Extensions: 173983077
Number of sequences better than 0.0: 0
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