BLASTX 7.6.2
Query= UN08656 /QuerySize=1197
(1196 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 224 1e-057
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 215 9e-055
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 214 2e-054
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 213 3e-054
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 210 3e-053
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 210 3e-053
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 209 4e-053
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 209 5e-053
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 205 5e-052
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 205 9e-052
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 178 9e-044
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 170 2e-041
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 165 8e-040
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 136 3e-031
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 126 4e-028
sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS... 121 1e-026
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 111 1e-023
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 107 2e-022
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 104 2e-021
sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens... 98 1e-019
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 224 bits (570), Expect = 1e-057
Identities = 112/257 (43%), Positives = 148/257 (57%), Gaps = 4/257 (1%)
Frame = -2
Query: 1060 VESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQ-AKHNTPE 884
+E W V + + L G+FI HE +F S +PF + L YKIQ K N +
Sbjct: 9 IEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWK 68
Query: 883 AQGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVF 704
Q CI +L H V P+M K +G+ + PLPS + I F+IEDF F
Sbjct: 69 TQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYF 128
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
YW HR LH + YK++H VHH++A PFG+T+EYAHP E + LG T IGP L L TL
Sbjct: 129 YWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTL 188
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W WL R+ +TVE H GY FPW+P+ +P +GG+ FHD+HH T GNYSSTF Y+D
Sbjct: 189 WVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDK 245
Query: 343 IFGTDKGYRKLKALKET 293
IFGT Y K ++++
Sbjct: 246 IFGTSDKYYSRKQIRDS 262
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 215 bits (545), Expect = 9e-055
Identities = 105/244 (43%), Positives = 141/244 (57%), Gaps = 4/244 (1%)
Frame = -2
Query: 1060 VESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKI-QAKHNTPE 884
+E W V + + L G+FI H+ +F +PF+ + YKI Q K N +
Sbjct: 9 IEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKENDWK 68
Query: 883 AQGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVF 704
+Q C ++ + P+M K +G+ + PLPS + ++ FIIEDF F
Sbjct: 69 SQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIEDFYF 128
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
YW HR LH YK +H VHH+YA+PFG+T+EYAHP E + LG T IGP L L TL
Sbjct: 129 YWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTL 188
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W WL +R+ +TVE H GY FPWS +N +P +GGA FHDYHH + GNY+STF Y+D
Sbjct: 189 WVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEVFI---GNYASTFTYLDK 245
Query: 343 IFGT 332
IFGT
Sbjct: 246 IFGT 249
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 214 bits (543), Expect = 2e-054
Identities = 107/262 (40%), Positives = 154/262 (58%), Gaps = 10/262 (3%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHE ++FF LP + + YKIQ K T E
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFFFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSF-PLPSWKVVSAQILFFFIIEDFVF 704
Q KC LLF H C+ FPL+ +Y ++ + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQFPLICGTYYFTEYFSIPYDWETMPRWYIALARCFGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H +HHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLILGTGFFIGIMLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R+IET++ H GY P +P + +P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTVRLIETIDVHSGYDIPLNPLHLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGY-----RKLKALKET 293
IFGTD Y ++ KA K+T
Sbjct: 271 IFGTDAQYNAYNEKRKKAEKKT 292
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 213 bits (540), Expect = 3e-054
Identities = 104/248 (41%), Positives = 147/248 (59%), Gaps = 5/248 (2%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHET++F LP + + YKIQ K T E
Sbjct: 34 KNAWVYMLDNYTKFQIATWGSLIVHETIYFLFSLPGFLFQFIPFMRKYKIQKDKPETFEG 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFP-LPSWKVVSAQILFFFIIEDFVF 704
Q KC+ +LF H + PL+ +Y +F + + +P W A+ L +IED
Sbjct: 94 QWKCLKGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTLARCLGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R++ET++ H GY P +P N++P Y GA HD+HH GNY+STF + D
Sbjct: 214 WAWVTMRLLETIDVHSGYDIPLNPLNYIPFYTGARHHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGY 320
IFGTD Y
Sbjct: 271 IFGTDVQY 278
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 210 bits (532), Expect = 3e-053
Identities = 102/248 (41%), Positives = 145/248 (58%), Gaps = 5/248 (2%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHE ++F LP + + YKIQ K T E
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFP-LPSWKVVSAQILFFFIIEDFVF 704
Q KC LLF H C+ PL+ +Y ++ + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R++ET++ H GY P +P N +P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGY 320
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 210 bits (532), Expect = 3e-053
Identities = 102/248 (41%), Positives = 145/248 (58%), Gaps = 5/248 (2%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHE ++F LP + + YKIQ K T E
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFP-LPSWKVVSAQILFFFIIEDFVF 704
Q KC LLF H C+ PL+ +Y ++ + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R++ET++ H GY P +P N +P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGY 320
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 209 bits (531), Expect = 4e-053
Identities = 102/248 (41%), Positives = 145/248 (58%), Gaps = 5/248 (2%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHE ++F LP + + YKIQ K T E
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFSFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFP-LPSWKVVSAQILFFFIIEDFVF 704
Q KC LLF H C+ PL+ +Y ++ + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R++ET++ H GY P +P N +P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGY 320
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 209 bits (530), Expect = 5e-053
Identities = 102/248 (41%), Positives = 146/248 (58%), Gaps = 5/248 (2%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS IVHE ++F LP + + YKIQ K T E
Sbjct: 34 KNAWVYMLDNYTKFQIATWGSLIVHEAIYFLFSLPGFLFQFIPYMRKYKIQKDKPETFEG 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFP-LPSWKVVSAQILFFFIIEDFVF 704
Q KC+ +LF H + PL+ +Y +F + + +P W + A+ L +IED
Sbjct: 94 QWKCLKKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTLARCLGCAVIEDTWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG FIG L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W+ +R++ET++ H GY P +P N +P Y GA HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLVPFYTGARHHDFHHMNFI---GNYASTFTWWDK 270
Query: 343 IFGTDKGY 320
+FGTD Y
Sbjct: 271 LFGTDAQY 278
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 205 bits (521), Expect = 5e-052
Identities = 99/247 (40%), Positives = 142/247 (57%), Gaps = 5/247 (2%)
Frame = -2
Query: 1048 WQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEAQGK 872
W +++ N++ FQ+A GS IVHE ++F LP + + YKIQ K T E Q K
Sbjct: 37 WNHMLQNYTKFQIATWGSLIVHELIYFLFCLPGFIFQFLPFMQKYKIQPDKPETWEKQWK 96
Query: 871 CIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSF-PLPSWKVVSAQILFFFIIEDFVFYWG 695
C LLF H C+ PL+ +Y +F + + +P W + AQ +IED Y+
Sbjct: 97 CFKMLLFNHFCIQLPLICGTYYFTEFFSIPYDWDTMPRWPFLLAQCFGCAVIEDTWHYFL 156
Query: 694 HRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITLWSW 515
HR LH + +YK +H VHH++ +PFG+ +EYAHP E L LG FIG + H+I LW+W
Sbjct: 157 HRALHHRRIYKYIHKVHHDFTSPFGMQAEYAHPLETLILGAGFFIGTMVFCNHMILLWAW 216
Query: 514 LVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFG 335
+ R++ET++ H GY P +P + +P Y GA FHD+HH GNY STF + D +F
Sbjct: 217 VTFRLLETIDVHSGYDIPLNPLHLIPFYAGARFHDFHHMNFV---GNYGSTFTWWDRLFD 273
Query: 334 TDKGYRK 314
TD + K
Sbjct: 274 TDSQFNK 280
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 205 bits (519), Expect = 9e-052
Identities = 101/260 (38%), Positives = 151/260 (58%), Gaps = 5/260 (1%)
Frame = -2
Query: 1057 ESGWQYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERTGLLNSYKIQA-KHNTPEA 881
++ W Y++ N++ FQ+A GS +VHE +F +P + + YKIQ K T E
Sbjct: 34 KNAWNYMLDNYTKFQIATWGSLLVHEASYFLLCVPGFIFQFIPYMQKYKIQPDKPETWEK 93
Query: 880 QGKCIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSF-PLPSWKVVSAQILFFFIIEDFVF 704
Q KC L+F H + PL+ +Y ++ + + +P W V+ AQ +IED
Sbjct: 94 QWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWYVLLAQCFGCAVIEDAWH 153
Query: 703 YWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITL 524
Y+ HR+LH K +YK +H VHHE+ +PFG+ +EYAHP E L LG FIG + H++ L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILGAGFFIGIVVFCNHVVLL 213
Query: 523 WSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDW 344
W+W++ R++ET++ H GY P +P + +P Y GA FHD+HH GNY+STF + D
Sbjct: 214 WAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMNFI---GNYASTFTWWDR 270
Query: 343 IFGTDKGYRKLKALKET*QL 284
IFGTD + K ++ QL
Sbjct: 271 IFGTDSQFIAYKEKEKKQQL 290
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 178 bits (450), Expect = 9e-044
Identities = 88/237 (37%), Positives = 131/237 (55%), Gaps = 9/237 (3%)
Frame = -2
Query: 997 SFIVHETVFFFSGLPFIFLERTGLLNSYKIQAKH-NTPEAQGKCIAWLLFYHSCVNFPLM 821
SF++HE ++F +P++ ++ +KIQ K T Q +C +L H V P +
Sbjct: 52 SFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKKVPTLAEQWECTRLVLLSHFTVELPQI 111
Query: 820 LFSYRVFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVFYWGHRILHTKWLYKNVHSVHH 641
+ G+ +S P P + QI FF +ED YW HR+ H Y+ +H VHH
Sbjct: 112 WLFDPMCATFGLSTSVPFPPVTKMIWQITLFFFLEDTWHYWAHRLFHYGIFYRFIHKVHH 171
Query: 640 EYATPFGLTSEYAHPAEILFLGFATFIGPAL-----TGPHLITLWSWLVLRVIETVEAHC 476
Y+ PFGL++EYAHP EI+ LG T P + HL+T++ W+ LR+ + V++H
Sbjct: 172 RYSAPFGLSAEYAHPLEIILLGAGTVFVPLMWCYFTHDLHLVTMYIWITLRLFQAVDSHA 231
Query: 475 GYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKA 305
GY FPWS + FLP++ GAD HDYHH N+SS+F + D + TD+ Y + KA
Sbjct: 232 GYDFPWSLNKFLPIWAGADHHDYHHMAF---KDNFSSSFRWWDAVLKTDQNYHQFKA 285
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 170 bits (430), Expect = 2e-041
Identities = 89/231 (38%), Positives = 132/231 (57%), Gaps = 11/231 (4%)
Frame = -2
Query: 994 FIVHETVFFFSGLPFIFLERTGLLNSYKIQ-AKHNTPEAQGKCIAWLLFYHSCVN-FPLM 821
F++HE ++FF LP+ +++ +K+Q K + + Q C+ +L H V P+
Sbjct: 59 FLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKIPSAKEQLYCLKSVLLSHFLVEAIPIW 118
Query: 820 LFSYRVFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVFYWGHRILHTKWLYKNVHSVHH 641
F + + + +G+ P PS K ++ +I FF++ED YW HR+ H YK +H HH
Sbjct: 119 TF-HPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHH 177
Query: 640 EYATPFGLTSEYAHPAEILFLGFATFIGPAL----TGP-HLITLWSWLVLRVIETVEAHC 476
YA PFGL++EYAHPAE L LGF T P L TG HL TL W+ LR+ + V++H
Sbjct: 178 RYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHS 237
Query: 475 GYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKG 323
GY FPWS + +P + GA+ HD HH GNY+S+F + D+ T+ G
Sbjct: 238 GYDFPWSLNKIMPFWAGAEHHDLHHHYFI---GNYASSFRWWDYCLDTESG 285
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 165 bits (416), Expect = 8e-040
Identities = 100/279 (35%), Positives = 145/279 (51%), Gaps = 14/279 (5%)
Frame = -2
Query: 1102 FSFLSFSLYQMDSL--VESGW-QYLVTNFSDFQLACIGSFIVHETVFFFSGLPFIFLERT 932
FS + + Q+D+L E W Y +D + F+ HE +F LP+ ++R
Sbjct: 20 FSQVYQNFNQLDNLNVFEKLWGSYYYYMANDLFATGLLFFLTHEIFYFGRCLPWAIIDRI 79
Query: 931 GLLNSYKIQ-AKHNTPEAQGKCIAWLLFYHSCVN-FPLMLFSYRVFKFMGMRSSFPLPSW 758
+KIQ K + + Q +C+ +L H V FP+ F + + + +G+ P P
Sbjct: 80 PYFRKWKIQDEKIPSDKEQWECLKSVLTSHFLVEAFPIWFF-HPLCQKIGISYQVPFPKI 138
Query: 757 KVVSAQILFFFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFL 578
+ Q FF++ED YW HR LH YK +H HH YA PFGL +EYAHP E+ L
Sbjct: 139 TDMLIQWAVFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVEVALL 198
Query: 577 GFAT----FIGPALTGP-HLITLWSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFH 413
G T + +TG HL T+ W++LR+ + V+AH GY FPWS NFLP + GAD H
Sbjct: 199 GLGTVGIPIVWCLITGNLHLFTVSIWIILRLFQAVDAHSGYEFPWSLHNFLPFWAGADHH 258
Query: 412 DYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKE 296
D HH G YSS+F + D+I T+ G + K ++
Sbjct: 259 DEHHHYFI---GGYSSSFRWWDFILDTEAGPKAKKGRED 294
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 136 bits (342), Expect = 3e-031
Identities = 85/264 (32%), Positives = 135/264 (51%), Gaps = 23/264 (8%)
Frame = -2
Query: 1057 ESGWQYLVTNF--SDFQLACIGSFIVHETVFF-FSGLPFIFLERTG---LLNSYKIQAKH 896
++ W+ L+T F ++ L IG+ V F+ F+GL + ++ TG ++ Y+IQ
Sbjct: 61 QAQWERLLTTFEGKEWILFFIGAIQVPCLFFWSFNGL-LLVVDTTGKPNFISRYRIQVGK 119
Query: 895 NTPEAQGK---CIAWLLFYHSCVNFPLMLFSYRVFKFMGMRSSFPLPSWKVVSAQILFFF 725
N P K I +LF V+FP+++F Y K+ G LP++ ++ F
Sbjct: 120 NEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLKWWGDPCRRELPTFHWFLLELAIFT 179
Query: 724 IIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAE-ILFLGFATFIGPAL 548
+IE+ +FY+ HR+LH YK +H HHE+ P G+ S YAHP E ++ +GP +
Sbjct: 180 LIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEHVVSNMLPAIVGPLV 239
Query: 547 TGPHLITLWSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYS 368
G HL ++ W L +I T +HCGYH P+ PS +FHDYHH K
Sbjct: 240 MGSHLSSITMWFSLALIITTISHCGYHLPFLPS--------PEFHDYHH----LKFNQCY 287
Query: 367 STFVYMDWIFGTDKGYRKLKALKE 296
+D + GTD +++ KA ++
Sbjct: 288 GVLGVLDHLHGTDTMFKQTKAYEK 311
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 126 bits (315), Expect = 4e-028
Identities = 74/228 (32%), Positives = 113/228 (49%), Gaps = 20/228 (8%)
Frame = -2
Query: 967 FSGLPFIFLERTG---LLNSYKIQAKHNTPEAQGK---CIAWLLFYHSCVNFPLMLFSYR 806
F+GL + ++ TG ++ Y+IQ N P K I +LF ++FP+++F Y
Sbjct: 94 FNGL-LLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYP 152
Query: 805 VFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATP 626
K+ LP++ ++ F +IE+ +FY+ HR+LH YK +H HHE+ P
Sbjct: 153 FLKWWRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAP 212
Query: 625 FGLTSEYAHPAEILFLG-FATFIGPALTGPHLITLWSWLVLRVIETVEAHCGYHFPWSPS 449
G+ S YAHP E +GP + G HL ++ W L +I T +HCGYH P+ PS
Sbjct: 213 IGVISLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLPFLPS 272
Query: 448 NFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKA 305
+FHDYHH K +D + GTD +++ KA
Sbjct: 273 --------PEFHDYHH----LKFNQCYGVLGVLDHLHGTDTMFKQTKA 308
>sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS=Danio rerio
GN=ch25h PE=2 SV=1
Length = 251
Score = 121 bits (303), Expect = 1e-026
Identities = 75/226 (33%), Positives = 111/226 (49%), Gaps = 18/226 (7%)
Frame = -2
Query: 979 TVFFFSGLPFIFLE----RTGLLNSYKIQAKHNTP-EAQGKCIAWLLFYHSCVNFPLML- 818
TV+ LPF+ L+ + L+ YKIQ K + C+A L+ H FPL +
Sbjct: 30 TVYLSFCLPFVLLDALSPKVELIRRYKIQQKASVSWTMMWSCLALSLYNHVVYIFPLSVL 89
Query: 817 -FSYRVFKFMGMRSSFPLPSWKVVSAQILFFFIIEDFVFYWGHRILH-TKWLYKNVHSVH 644
+ +R ++ W + + +LF DF ++ H + H WLY+ H VH
Sbjct: 90 HWYWRPVSYLAEAPGVLRVVWDLAACLLLF-----DFQYFVWHLLHHKVPWLYRTFHKVH 144
Query: 643 HEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITLWSWLVLRVIETVEAHCGYHF 464
H+Y + F L +EY+ E L LGF + P L G H +T + +L + +VE HCGY
Sbjct: 145 HKYTSTFALATEYSGAWETLSLGFFAAVNPMLLGVHPMTEMLFHMLNMWLSVEDHCGYDL 204
Query: 463 PWSPSNFLP--LYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 332
PW+ +P LYGGA HD HH+ NY+ F + D +FGT
Sbjct: 205 PWATHRLMPFGLYGGAPHHDVHHQKF---KSNYAPYFTHWDKLFGT 247
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 111 bits (276), Expect = 1e-023
Identities = 71/233 (30%), Positives = 112/233 (48%), Gaps = 25/233 (10%)
Frame = -2
Query: 973 FFFSGLPFIFLERTG----LLNSYKIQA-KHNTPEAQGKCIAWLLFYHSCVNFPLMLFSY 809
+F LP++ + G + YKIQ K T C L+ H + P + +
Sbjct: 49 YFVLVLPYLSCDILGRKWPAIYRYKIQPDKLPTTAMLLHCSGVTLYNHILLVIPAAVAQW 108
Query: 808 RVFKFMGMRSSFPLPSWKVVSAQI-------LFFFIIEDFVFYWGHRILHTKWLYKNVHS 650
R PLP ++ L F ++ F++++ H + +WLY H+
Sbjct: 109 M------WRPPIPLPEQAPTLLELVGGVTGNLLLFDLQYFIWHFLHHKI--RWLYVTFHA 160
Query: 649 VHHEYATPFGLTSEYAHPAEILFLGFATFIGPALTGPHLITLWSWLVLRVIETVEAHCGY 470
+HH Y+ PF L ++ E++ +GF T + P L HL+T W ++V+ V +VE HCGY
Sbjct: 161 IHHNYSAPFALATQCLGGWELVTVGFWTTLNPVLLRCHLLTTWMFMVVHVYVSVEDHCGY 220
Query: 469 HFPWSPSNFLP--LYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYR 317
FPWS S +P +YGG HD HH+ T N++ F + D +FGT +R
Sbjct: 221 DFPWSTSRLIPFGVYGGPSKHDVHHQKPNT---NFAPHFSHWDKMFGTHADFR 270
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 107 bits (266), Expect = 2e-022
Identities = 57/164 (34%), Positives = 82/164 (50%), Gaps = 5/164 (3%)
Frame = -2
Query: 745 AQILFFFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFA 569
+Q+ FF+ DF ++W H I H KWLY+ HSVHH Y++PF ++++ HP E+ F+G
Sbjct: 132 SQLAIFFLAFDFTYFWFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVGTF 191
Query: 568 TFIGPALTGPHLITLWSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLY 389
P + H +T W W + + E H GY FP++ Y GA HD HH
Sbjct: 192 ITTIPWIFPTHCLTYWIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHLRPL 251
Query: 388 TKSGNYSSTFVYMDWIFGTDKGYRKLKALKET*QLVKLGLSRPE 257
T + F Y+D + G Y LK + E + K GL E
Sbjct: 252 T---CFQPWFNYLDRLMGYHITYADLKKMTEA-KFKKFGLYSAE 291
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 104 bits (257), Expect = 2e-021
Identities = 58/162 (35%), Positives = 83/162 (51%), Gaps = 9/162 (5%)
Frame = -2
Query: 745 AQILFFFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFA 569
+Q+ FF+ DF ++W H H KWLY+ HSVHH Y++PF ++++ HP E+ F+ A
Sbjct: 132 SQLAIFFLAFDFTYFWFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFV--A 189
Query: 568 TFIG--PALTGPHLITLWSWLVLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRL 395
TFI P + H +T W W + + E H GY FP++ Y GA HD HH
Sbjct: 190 TFITTVPWIFPTHCLTYWLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHLR 249
Query: 394 LYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET*QLVKLGL 269
T + F Y+D + G Y LK + E + K GL
Sbjct: 250 PLT---CFQPWFNYLDRLMGYHITYEDLKKMTEA-KFKKFGL 287
>sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens GN=CH25H PE=1
SV=1
Length = 272
Score = 98 bits (242), Expect = 1e-019
Identities = 67/218 (30%), Positives = 102/218 (46%), Gaps = 16/218 (7%)
Frame = -2
Query: 958 LPFIFLE----RTGLLNSYKIQAKHNTPEAQG--KCIAWLLFYHSCVNFPLMLFSYRVFK 797
LPF+ L+ L YKI +P AQ C+ L+ H FP+ L +
Sbjct: 53 LPFVVLDILCSWVPALRRYKIHPDF-SPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSP 111
Query: 796 FMGMRSSFPLPSWKVVSAQILFFFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFG 620
+ + P ++ ILF ++ D F+ H + H WLY+ H VHH+ ++ F
Sbjct: 112 ALLPHEA---PELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFA 168
Query: 619 LTSEYAHPAEILFLGFATFIGPALTGPHLITLWSWLVLRVIETVEAHCGYHFPWSPSNFL 440
L ++Y E+ LGF + L G H +T ++ V+ + +VE H GY+FPWS +
Sbjct: 169 LATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLV 228
Query: 439 PL--YGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 332
P YGG HD HH + N++ F + D I GT
Sbjct: 229 PFGWYGGVVHHDLHHSHF---NCNFAPYFTHWDKILGT 263
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,801,685,782
Number of Sequences: 518415
Number of Extensions: 36801685782
Number of Successful Extensions: 258262551
Number of sequences better than 0.0: 0
|
