BLASTX 7.6.2
Query= UN10544 /QuerySize=1427
(1426 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 193 5e-048
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 181 1e-044
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 170 3e-041
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 160 3e-038
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 159 6e-038
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 149 7e-035
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 148 2e-034
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 147 2e-034
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 147 2e-034
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 147 3e-034
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 146 5e-034
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 145 1e-033
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 143 5e-033
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 114 2e-024
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 112 6e-024
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 112 8e-024
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 105 9e-022
sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegi... 100 3e-020
sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus... 99 7e-020
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 95 1e-018
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 193 bits (488), Expect = 5e-048
Identities = 95/264 (35%), Positives = 146/264 (55%), Gaps = 12/264 (4%)
Frame = -2
Query: 1242 NLTWLETVWFDYSATKSDYYLYCHNILFLFLVFSLAP-LPLVLVELARSASGWFDRYKIQ 1066
+L ++E WF + + +L + VF +P L + + +YKIQ
Sbjct: 5 SLKFIEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDF----MPFLQKYKIQ 60
Query: 1065 PKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPLPTFSEMLPQLFVY 886
P F C V+ I V P+ + P+I+ IG+ + PLP+ ++ +
Sbjct: 61 PTKKNEWKTQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACC 120
Query: 885 FLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAI 706
FLIED+ YWVHR H + Y++IH+VHH++ AP G A YAH E ++LG+ T +GP +
Sbjct: 121 FLIEDFYFYWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFL 180
Query: 705 APGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH--YVGGQSHSN 532
+ T W+W+ R + +E HSGYDFPW+PTK IPF+GG+ +HD+HH +VG N
Sbjct: 181 FSRDLFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHETFVG-----N 235
Query: 531 FASVFTYCDYIYGTDKGYRFQKKL 460
++S FTY D I+GT Y +K++
Sbjct: 236 YSSTFTYLDKIFGTSDKYYSRKQI 259
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 181 bits (459), Expect = 1e-044
Identities = 96/268 (35%), Positives = 144/268 (53%), Gaps = 11/268 (4%)
Frame = -2
Query: 1242 NLTWLETVWFDYSATKSDYYLYCHNILFLFLVFSLAP-LPLVLVELARSASGWFDRYKIQ 1066
+L ++E WF + + +L + VF +P ++ + +F +YKIQ
Sbjct: 5 SLKFIEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFI----PFFRKYKIQ 60
Query: 1065 PKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPLPTFSEMLPQLFVY 886
C V+ +L+ P+ + P+I+ IG+ + PLP+ +L L
Sbjct: 61 QTKENDWKSQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSS 120
Query: 885 FLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAI 706
F+IED+ YW HR H Y+ IH+VHH+Y +P G A YAH E ++LG+ T +GP +
Sbjct: 121 FIIEDFYFYWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFL 180
Query: 705 APGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSHSNFA 526
+ T W+W+ +R + +E HSGYDFPWS T IPF+GGA +HDYHH V N+A
Sbjct: 181 FSRDLFTLWVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEV---FIGNYA 237
Query: 525 SVFTYCDYIYGTD-KGY--RFQKKLLEQ 451
S FTY D I+GT K Y R +KK + +
Sbjct: 238 STFTYLDKIFGTSGKSYYSRIEKKSINK 265
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 170 bits (429), Expect = 3e-041
Identities = 94/283 (33%), Positives = 152/283 (53%), Gaps = 19/283 (6%)
Frame = -2
Query: 1239 LTWLETVWFDYSATKSDYYLYCHNI----LFLFLVFSLAPLPLVLVELARSASGWFDRYK 1072
L ++E +W Y Y + +++ L FL+ L + + A +F R+K
Sbjct: 27 LNFVEQLWLAY------YKWFDNDVVATGLMSFLLHELIYFGRCIPWMIIDAMPYFRRWK 80
Query: 1071 IQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPLPTFSEMLPQLF 892
IQPK +L++ + C + V+ V P + P G+ + +P P ++M+ Q+
Sbjct: 81 IQPKKVPTLAEQWECTRLVLLSHFTVELPQIWLFDPMCATFGLSTSVPFPPVTKMIWQIT 140
Query: 891 VYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGP 712
++F +ED +YW HR FH Y IH+VHH Y+AP G +A YAH E++LLG T P
Sbjct: 141 LFFFLEDTWHYWAHRLFHYGIFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVP 200
Query: 711 AI-----APGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGG 547
+ H++T ++WI LR +A+++H+GYDFPWS K++P + GA++HDYHH
Sbjct: 201 LMWCYFTHDLHLVTMYIWITLRLFQAVDSHAGYDFPWSLNKFLPIWAGADHHDYHHMA-- 258
Query: 546 QSHSNFASVFTYCDYIYGTDKGY-RFQKKLLEQIKESSKKSNK 421
NF+S F + D + TD+ Y +F+ + L E+ K K
Sbjct: 259 -FKDNFSSSFRWWDAVLKTDQNYHQFKARRLAAKYEAESKKAK 300
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 160 bits (403), Expect = 3e-038
Identities = 84/227 (37%), Positives = 125/227 (55%), Gaps = 10/227 (4%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPLPTFSE 910
+F R+K+QP S + C K V+ LV +P + +GI +P P+
Sbjct: 81 YFRRWKLQPTKIPSAKEQLYCLKSVLLSHFLVEAIPIWTFHPMCEKLGITVEVPFPSLKT 140
Query: 909 MLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGI 730
M ++ ++F++ED +YW HR FH Y+ IH+ HH Y AP G +A YAH AE L LG
Sbjct: 141 MALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGF 200
Query: 729 PTFMGPAI-----APGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHD- 568
T P + H+ T +WI LR +A+++HSGYDFPWS K +PF+ GAE+HD
Sbjct: 201 GTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDL 260
Query: 567 YHHYVGGQSHSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKS 427
+HHY G N+AS F + DY T+ G + E++K+ ++ +
Sbjct: 261 HHHYFIG----NYASSFRWWDYCLDTESGPEAKASREERMKKRAENN 303
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 159 bits (401), Expect = 6e-038
Identities = 96/282 (34%), Positives = 148/282 (52%), Gaps = 14/282 (4%)
Frame = -2
Query: 1242 NLTWLETVWFDYSATKSDYYLYCHNILFLFLVFSLAPLPLVLVELARSASGWFDRYKIQP 1063
NL E +W Y ++ L+ +LF FL + L +F ++KIQ
Sbjct: 32 NLNVFEKLWGSYYYYMAN-DLFATGLLF-FLTHEIFYFGRCLPWAIIDRIPYFRKWKIQD 89
Query: 1062 KVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPLPTFSEMLPQLFVYF 883
+ S + + C K V+ LV +P Q IGI +P P ++ML Q V+F
Sbjct: 90 EKIPSDKEQWECLKSVLTSHFLVEAFPIWFFHPLCQKIGISYQVPFPKITDMLIQWAVFF 149
Query: 882 LIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAI- 706
++ED +YW HR H Y+ IH+ HH Y AP G AA YAH EV LLG+ T P +
Sbjct: 150 VLEDTWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGTVGIPIVW 209
Query: 705 ----APGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH--YVGGQ 544
H+ T +WI LR +A++ HSGY+FPWS ++PF+ GA++HD HH ++GG
Sbjct: 210 CLITGNLHLFTVSIWIILRLFQAVDAHSGYEFPWSLHNFLPFWAGADHHDEHHHYFIGG- 268
Query: 543 SHSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKQ 418
++S F + D+I T+ G + +K +++K++ +K K+
Sbjct: 269 ----YSSSFRWWDFILDTEAGPKAKKGREDKVKQNVEKLQKK 306
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 149 bits (374), Expect = 7e-035
Identities = 75/207 (36%), Positives = 114/207 (55%), Gaps = 8/207 (3%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGI-RSGLPLPTFS 913
+ +YKIQP + + C+K ++ + PL +Y + I +P +
Sbjct: 77 YMQKYKIQPDKPETWEKQWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWY 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
+L Q F +IED +Y++HR H K Y+ IH+VHHE+ +P G A YAH E L+LG
Sbjct: 137 VLLAQCFGCAVIEDAWHYFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + H++ W W+ R +E I+ HSGYD P +P +PFY GA +HD+HH
Sbjct: 197 AGFFIGIVVFCNHVVLLWAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGY 478
++G N+AS FT+ D I+GTD +
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQF 278
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 148 bits (371), Expect = 2e-034
Identities = 78/214 (36%), Positives = 119/214 (55%), Gaps = 10/214 (4%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLP-LPTFS 913
+ +YKIQ + + + C+K ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + H+I W W+ +R +E I+ HSGYD P +P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGYR--FQKK 463
++G N+AS FT+ D I+GTD Y ++KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYHAYYEKK 285
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 147 bits (370), Expect = 2e-034
Identities = 79/220 (35%), Positives = 120/220 (54%), Gaps = 16/220 (7%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLP-LPTFS 913
+ +YKIQ + + + C+K ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + H+I W W+ +R +E I+ HSGYD P +P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGY--------RFQKK 463
++G N+AS FT+ D I+GTD Y +F+KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYNAYNEKRKKFEKK 291
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 147 bits (370), Expect = 2e-034
Identities = 79/220 (35%), Positives = 120/220 (54%), Gaps = 16/220 (7%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLP-LPTFS 913
+ +YKIQ + + + C+K ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + H+I W W+ +R +E I+ HSGYD P +P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGY--------RFQKK 463
++G N+AS FT+ D I+GTD Y +F+KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYNAYNEKRKKFEKK 291
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 147 bits (369), Expect = 3e-034
Identities = 77/214 (35%), Positives = 116/214 (54%), Gaps = 8/214 (3%)
Frame = -2
Query: 1080 RYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLP-LPTFSEML 904
+YKIQ + + C K ++ + PL +Y + I +P + L
Sbjct: 80 KYKIQKDKPETFEGQWKCLKGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTL 139
Query: 903 PQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPT 724
+ +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 140 ARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGF 199
Query: 723 FMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH--YVG 550
F+G + H+I W W+ +R +E I+ HSGYD P +P YIPFY GA +HD+HH ++G
Sbjct: 200 FIGIVLLCDHVILLWAWVTMRLLETIDVHSGYDIPLNPLNYIPFYTGARHHDFHHMNFIG 259
Query: 549 GQSHSNFASVFTYCDYIYGTDKGYRFQKKLLEQI 448
N+AS FT+ D I+GTD Y + ++++
Sbjct: 260 -----NYASTFTWWDRIFGTDVQYHAYTEKMKKL 288
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 146 bits (367), Expect = 5e-034
Identities = 75/207 (36%), Positives = 113/207 (54%), Gaps = 8/207 (3%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGL-PLPTFS 913
+ +YKIQP + + C+K ++ + PL +Y + I +P +
Sbjct: 77 FMQKYKIQPDKPETWEKQWKCFKMLLFNHFCIQLPLICGTYYFTEFFSIPYDWDTMPRWP 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
+L Q F +IED +Y++HR H + Y+ IH+VHH++T+P G A YAH E L+LG
Sbjct: 137 FLLAQCFGCAVIEDTWHYFLHRALHHRRIYKYIHKVHHDFTSPFGMQAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + HMI W W+ R +E I+ HSGYD P +P IPFY GA +HD+HH
Sbjct: 197 AGFFIGTMVFCNHMILLWAWVTFRLLETIDVHSGYDIPLNPLHLIPFYAGARFHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGY 478
+VG N+ S FT+ D ++ TD +
Sbjct: 257 FVG-----NYGSTFTWWDRLFDTDSQF 278
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 145 bits (364), Expect = 1e-033
Identities = 76/207 (36%), Positives = 114/207 (55%), Gaps = 8/207 (3%)
Frame = -2
Query: 1089 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGI-RSGLPLPTFS 913
+ +YKIQ + + + C+K ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQFPLICGTYYFTEYFSIPYDWETMPRWY 136
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 733
L + F +IED +Y++HR H K Y+ IH++HHE+ AP G A YAH E L+LG
Sbjct: 137 IALARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLILG 196
Query: 732 IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH-- 559
F+G + H+I W W+ +R IE I+ HSGYD P +P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIMLLCDHVILLWAWVTVRLIETIDVHSGYDIPLNPLHLIPFYAGSRHHDFHHMN 256
Query: 558 YVGGQSHSNFASVFTYCDYIYGTDKGY 478
++G N+AS FT+ D I+GTD Y
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDAQY 278
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 143 bits (358), Expect = 5e-033
Identities = 74/204 (36%), Positives = 110/204 (53%), Gaps = 8/204 (3%)
Frame = -2
Query: 1080 RYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLP-LPTFSEML 904
+YKIQ + + C K ++ + PL +Y + I +P + L
Sbjct: 80 KYKIQKDKPETFEGQWKCLKKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTL 139
Query: 903 PQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPT 724
+ +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 140 ARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGF 199
Query: 723 FMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHH--YVG 550
F+G + H+I W W+ +R +E I+ HSGYD P +P +PFY GA +HD+HH ++G
Sbjct: 200 FIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLVPFYTGARHHDFHHMNFIG 259
Query: 549 GQSHSNFASVFTYCDYIYGTDKGY 478
N+AS FT+ D ++GTD Y
Sbjct: 260 -----NYASTFTWWDKLFGTDAQY 278
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 114 bits (284), Expect = 2e-024
Identities = 71/196 (36%), Positives = 102/196 (52%), Gaps = 10/196 (5%)
Frame = -2
Query: 1020 DVMKMFILVVGPLQLV-SYPS--IQMIGI-RSGLPL--PTFSEMLPQLFVYFLIEDYTNY 859
D+MK + + G QL+ YP +Q+I + + LP+ PT EM+ QL ++FL D+T +
Sbjct: 87 DLMKKSLKLQGWNQLLWIYPMALVQLIWVPDTELPILAPTVFEMVSQLAIFFLAFDFTYF 146
Query: 858 WVHRFFHS-KWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITF 682
W H F H KW Y H VHH Y++P +A + H E+ + P I P H +T+
Sbjct: 147 WFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVATFITTVPWIFPTHCLTY 206
Query: 681 WLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSHSNFASVFTYCDY 502
WLW + Q + E H GYDFP++ + FY GA HD HH + + F F Y D
Sbjct: 207 WLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHL---RPLTCFQPWFNYLDR 263
Query: 501 IYGTDKGYRFQKKLLE 454
+ G Y KK+ E
Sbjct: 264 LMGYHITYEDLKKMTE 279
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 112 bits (280), Expect = 6e-024
Identities = 70/196 (35%), Positives = 102/196 (52%), Gaps = 10/196 (5%)
Frame = -2
Query: 1020 DVMKMFILVVGPLQLV-SYPS--IQMIGI-RSGLPL--PTFSEMLPQLFVYFLIEDYTNY 859
D++K + + G QL+ YP +Q+I + + LP+ PT EML QL ++FL D+T +
Sbjct: 87 DLIKKSLKLQGWNQLLWIYPMALVQLIWVPDTELPILAPTVFEMLSQLAIFFLAFDFTYF 146
Query: 858 WVHRFFHS-KWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITF 682
W H H KW Y H VHH Y++P +A + H E+ +G P I P H +T+
Sbjct: 147 WFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVGTFITTIPWIFPTHCLTY 206
Query: 681 WLWIALRQIEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSHSNFASVFTYCDY 502
W+W + Q + E H GYDFP++ + FY GA HD HH + + F F Y D
Sbjct: 207 WIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHL---RPLTCFQPWFNYLDR 263
Query: 501 IYGTDKGYRFQKKLLE 454
+ G Y KK+ E
Sbjct: 264 LMGYHITYADLKKMTE 279
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 112 bits (279), Expect = 8e-024
Identities = 68/213 (31%), Positives = 107/213 (50%), Gaps = 18/213 (8%)
Frame = -2
Query: 1080 RYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQMIGIRSGLPL----PTFS 913
RYKIQP + + + C + IL+V P + + R +PL PT
Sbjct: 71 RYKIQPDKLPTTAMLLHCSGVTLYNHILLVIPAAVAQWM------WRPPIPLPEQAPTLL 124
Query: 912 EMLPQLFVYFLIEDYTNYWVHRFFHSK--WGYENIHRVHHEYTAPIGYAAPYAHWAEVLL 739
E++ + L+ D Y++ F H K W Y H +HH Y+AP A E++
Sbjct: 125 ELVGGVTGNLLLFD-LQYFIWHFLHHKIRWLYVTFHAIHHNYSAPFALATQCLGGWELVT 183
Query: 738 LGIPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIPF--YGGAEYHDY 565
+G T + P + H++T W+++ + ++E H GYDFPWS ++ IPF YGG HD
Sbjct: 184 VGFWTTLNPVLLRCHLLTTWMFMVVHVYVSVEDHCGYDFPWSTSRLIPFGVYGGPSKHDV 243
Query: 564 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRFQK 466
HH + ++NFA F++ D ++GT +RF K
Sbjct: 244 HHQ---KPNTNFAPHFSHWDKMFGTHADFRFSK 273
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 105 bits (261), Expect = 9e-022
Identities = 68/223 (30%), Positives = 107/223 (47%), Gaps = 15/223 (6%)
Frame = -2
Query: 1125 LVLVELARSASGWFDRYKIQPKVNYSLS--DMFGCYKDVMKMFILVVGPLQLVSYPSIQM 952
L+LV + RY+IQ N + + + V+ +V P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLKW 156
Query: 951 IGIRSGLPLPTFSEMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYA 772
G LPTF L +L ++ LIE+ Y+ HR H Y+ IH+ HHE+TAPIG
Sbjct: 157 WGDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVI 216
Query: 771 APYAHWAE-VLLLGIPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIP 595
+ YAH E V+ +P +GP + H+ + +W +L I +H GY ++P
Sbjct: 217 SLYAHPIEHVVSNMLPAIVGPLVMGSHLSSITMWFSLALIITTISHCGY--------HLP 268
Query: 594 FYGGAEYHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRFQK 466
F E+HDYHH Q + V D+++GTD ++ K
Sbjct: 269 FLPSPEFHDYHHLKFNQCY----GVLGVLDHLHGTDTMFKQTK 307
>sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegicus GN=Ch25h
PE=2 SV=1
Length = 298
Score = 100 bits (248), Expect = 3e-020
Identities = 68/238 (28%), Positives = 111/238 (46%), Gaps = 12/238 (5%)
Frame = -2
Query: 1164 LFLFLVFSLAPLPLVLVELARSASGWFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGP 985
+F +L F LP V++++ RYKI P + S+ + C + ++ V P
Sbjct: 45 IFTYLGFC---LPFVVLDVLCPWVPILRRYKIHPDFSPSVRQLLPCLGLTLYQHLVFVFP 101
Query: 984 LQLVSYPSIQMIGIRSGLPLPTFSEMLPQLFVYFLIEDYTNYWVHRFFHS-KWGYENIHR 808
+ L+ + + R P S++L + + L+ D + H H W Y H+
Sbjct: 102 VTLMHWARSPALLPREA---PELSQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHK 158
Query: 807 VHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGY 628
VHH+ ++ A Y E+L L + A+ H +T ++ + ++E HSGY
Sbjct: 159 VHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWLSVEDHSGY 218
Query: 627 DFPWSPTKYIPF--YGGAEYHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRFQKKL 460
DFPWS + +PF YGG +HD HH Q + NFA FT+ D + GT + K+L
Sbjct: 219 DFPWSTHRLVPFGWYGGVAHHDLHH---SQFNCNFAPYFTHWDKMLGTLRCAPHSKRL 273
>sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus GN=Ch25h PE=1
SV=1
Length = 298
Score = 99 bits (245), Expect = 7e-020
Identities = 64/221 (28%), Positives = 104/221 (47%), Gaps = 17/221 (7%)
Frame = -2
Query: 1131 LPLVLVELARSASGWFDRYKIQPKVNYSLSDMFGCYKDVMKMFILVVGPLQLVSYPSIQM 952
LP V++++ RYKI P + S+ + C + ++ V P+ L+ +
Sbjct: 53 LPFVVLDVLYPWVPILRRYKIHPDFSPSVKQLLPCLGLTLYQHLVFVFPVTLLHW----- 107
Query: 951 IGIRSGLPL----PTFSEMLPQLFVYFLIEDYTNYWVHRFFHS-KWGYENIHRVHHEYTA 787
+RS L P ++L + + L+ D + H H W Y H+VHH+ ++
Sbjct: 108 --VRSPALLPQEAPELVQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSS 165
Query: 786 PIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPT 607
A Y + E+L L + A+ H +T + + + ++E HSGYDFPWS
Sbjct: 166 SFALATQYMSFWELLSLTFFDVLNVAVLRCHPLTIFTFHVINIWLSVEDHSGYDFPWSTH 225
Query: 606 KYIPF--YGGAEYHDYHHYVGGQSHSNFASVFTYCDYIYGT 490
+ +PF YGG +HD HH Q + NFA FT+ D + GT
Sbjct: 226 RLVPFGWYGGVAHHDMHH---SQFNCNFAPYFTHWDKMLGT 263
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 95 bits (234), Expect = 1e-018
Identities = 58/192 (30%), Positives = 92/192 (47%), Gaps = 11/192 (5%)
Frame = -2
Query: 1125 LVLVELARSASGWFDRYKIQPKVNYSLS--DMFGCYKDVMKMFILVVGPLQLVSYPSIQM 952
L+LV + RY+IQ N + + + V+ ++ P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKW 156
Query: 951 IGIRSGLPLPTFSEMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYA 772
LPTF L +L ++ LIE+ Y+ HR H Y+ IH+ HHE+TAPIG
Sbjct: 157 WRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVI 216
Query: 771 APYAHWAEVLLLG-IPTFMGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSPTKYIP 595
+ YAH E + +P +GP + H+ + +W +L I +H GY ++P
Sbjct: 217 SLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGY--------HLP 268
Query: 594 FYGGAEYHDYHH 559
F E+HDYHH
Sbjct: 269 FLPSPEFHDYHH 280
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,414,502,248
Number of Sequences: 518415
Number of Extensions: 42414502248
Number of Successful Extensions: 312188416
Number of sequences better than 0.0: 0
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