BLASTX 7.6.2
Query= UN10769 /QuerySize=1173
(1172 letters)
Database: TAIR9 protein;
33,410 sequences; 13,468,323 total letters
Score E
Sequences producing significant alignments: (bits) Value
TAIR9_protein||AT1G03310.2 | Symbols: ATISA2, ISA2, DBE1, BE2 | ... 606 1e-173
TAIR9_protein||AT1G03310.1 | Symbols: ATISA2, ISA2, DBE1, BE2 | ... 606 1e-173
TAIR9_protein||AT2G39930.1 | Symbols: ISA1, ATISA1 | ISA1 (ISOAM... 87 2e-017
TAIR9_protein||AT4G09020.1 | Symbols: ATISA3, ISA3 | ISA3 (ISOAM... 62 5e-010
>TAIR9_protein||AT1G03310.2 | Symbols: ATISA2, ISA2, DBE1, BE2 | isoamylase,
putative / starch debranching enzyme, putative | chr1:813975-816623
FORWARD
Length = 883
Score = 606 bits (1561), Expect = 1e-173
Identities = 298/357 (83%), Positives = 326/357 (91%), Gaps = 10/357 (2%)
Frame = +1
Query: 1 VLRGVHGEHLSRPPLVEAISFDPLLAGRKLIADCWDPHDMVTIPKEVRFPHWKRWRELNT 180
+LRGVHGE LSRPPLVEAI+FDPLLA KLIADCWDP +M +PKEVRFPHWKRW ELNT
Sbjct: 535 LLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEM--MPKEVRFPHWKRWAELNT 592
Query: 181 RYCRDVRNFVRGRGLLSDLATRICGSGDVFTDGRGPAFSFNYVSRNSGLSLVDLVSFSGS 360
RYCR+VRNF+RGRG+LSDLATRICGSGDVFTDGRGPAFSFNY+SRNSGLSLVD+VSFSG
Sbjct: 593 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSG- 651
Query: 361 PELSSELSWNCGEEGATNKSAVLQTRLKQIRNFLFIQYVSLGIPVLNMGDECGVSTKGSP 540
PEL+SELSWNCGEEGATNKSAVLQ RLKQIRNFLFIQY+SLG+PVLNMGDECG+ST+GSP
Sbjct: 652 PELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSP 711
Query: 541 LLESRKPFDWNMLASAFGSQITQFISCMTTVRARRGDVFQRRNFLKPENIVWHANDQTAP 720
LLESRKPFDWN+LASAFG+QITQFIS MT+VRARR DVFQRR+FLKPENIVW+ANDQT P
Sbjct: 712 LLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTP 771
Query: 721 KWEDSSSKFLALEIKAETVEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGR 900
KWED +SKFLALEIK+E+ EE TA S EP EPK+NDLFIGFNASD PE+V+LPSLPDG
Sbjct: 772 KWEDPASKFLALEIKSESEEEETA-SLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 830
Query: 901 EWRRLVDTALPFPGFFSVEGETVVAEEEEEEEEEMNQPVMYEMKPYSCTLFETINAT 1071
+WRRLVDTALPFPGFFSVEGETVVA EE + Q V+YEMKPYSCTLFETIN T
Sbjct: 831 KWRRLVDTALPFPGFFSVEGETVVA------EEPLQQLVVYEMKPYSCTLFETINTT 881
>TAIR9_protein||AT1G03310.1 | Symbols: ATISA2, ISA2, DBE1, BE2 | isoamylase,
putative / starch debranching enzyme, putative | chr1:813975-816623
FORWARD
Length = 883
Score = 606 bits (1561), Expect = 1e-173
Identities = 298/357 (83%), Positives = 326/357 (91%), Gaps = 10/357 (2%)
Frame = +1
Query: 1 VLRGVHGEHLSRPPLVEAISFDPLLAGRKLIADCWDPHDMVTIPKEVRFPHWKRWRELNT 180
+LRGVHGE LSRPPLVEAI+FDPLLA KLIADCWDP +M +PKEVRFPHWKRW ELNT
Sbjct: 535 LLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEM--MPKEVRFPHWKRWAELNT 592
Query: 181 RYCRDVRNFVRGRGLLSDLATRICGSGDVFTDGRGPAFSFNYVSRNSGLSLVDLVSFSGS 360
RYCR+VRNF+RGRG+LSDLATRICGSGDVFTDGRGPAFSFNY+SRNSGLSLVD+VSFSG
Sbjct: 593 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSG- 651
Query: 361 PELSSELSWNCGEEGATNKSAVLQTRLKQIRNFLFIQYVSLGIPVLNMGDECGVSTKGSP 540
PEL+SELSWNCGEEGATNKSAVLQ RLKQIRNFLFIQY+SLG+PVLNMGDECG+ST+GSP
Sbjct: 652 PELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSP 711
Query: 541 LLESRKPFDWNMLASAFGSQITQFISCMTTVRARRGDVFQRRNFLKPENIVWHANDQTAP 720
LLESRKPFDWN+LASAFG+QITQFIS MT+VRARR DVFQRR+FLKPENIVW+ANDQT P
Sbjct: 712 LLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTP 771
Query: 721 KWEDSSSKFLALEIKAETVEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGR 900
KWED +SKFLALEIK+E+ EE TA S EP EPK+NDLFIGFNASD PE+V+LPSLPDG
Sbjct: 772 KWEDPASKFLALEIKSESEEEETA-SLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 830
Query: 901 EWRRLVDTALPFPGFFSVEGETVVAEEEEEEEEEMNQPVMYEMKPYSCTLFETINAT 1071
+WRRLVDTALPFPGFFSVEGETVVA EE + Q V+YEMKPYSCTLFETIN T
Sbjct: 831 KWRRLVDTALPFPGFFSVEGETVVA------EEPLQQLVVYEMKPYSCTLFETINTT 881
>TAIR9_protein||AT2G39930.1 | Symbols: ISA1, ATISA1 | ISA1 (ISOAMYLASE 1);
alpha-amylase/ isoamylase | chr2:16666078-16672183 FORWARD
Length = 784
Score = 87 bits (213), Expect = 2e-017
Identities = 46/128 (35%), Positives = 71/128 (55%), Gaps = 4/128 (3%)
Frame = +1
Query: 19 GEHLSRPPLVEAISFDPLLAGRKLIADCWDPHDMVTIPKEVRFPHWKRWRELNTRYCRDV 198
G +S PP+++ IS DP+L G KLIA+ WD + + FPHW W E N ++ V
Sbjct: 440 GTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVG---MFPHWGIWSEWNGKFRDVV 496
Query: 199 RNFVRGR-GLLSDLATRICGSGDVFTDGRGPAFSFNYVSRNSGLSLVDLVSFSGSPELSS 375
R F++G G A +CGS +++ GR P S N++ + G +L DLV+++ L++
Sbjct: 497 RQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADLVTYNNKNNLAN 556
Query: 376 ELSWNCGE 399
N GE
Sbjct: 557 GEENNDGE 564
Score = 54 bits (129), Expect = 1e-007
Identities = 38/132 (28%), Positives = 59/132 (44%), Gaps = 9/132 (6%)
Frame = +1
Query: 382 SWNCGEEGATNKSAVLQTRLKQIRNFLFIQYVSLGIPVLNMGDECGVSTKGSPLL----E 549
SWNCGEEG +V + R +Q+RNF VS G+P++ MGDE G + G+
Sbjct: 569 SWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDN 628
Query: 550 SRKPFDWNMLASAFGSQITQFISCMTTVRAR-RGDVFQRRNFLKPENIVWHANDQTAPKW 726
F W+ A F C ++ R + +F + + WH P W
Sbjct: 629 YMNYFRWDKKEEAHSD---FFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNW 685
Query: 727 EDSSSKFLALEI 762
+ +S+F+A +
Sbjct: 686 SE-TSRFVAFSL 696
>TAIR9_protein||AT4G09020.1 | Symbols: ATISA3, ISA3 | ISA3 (ISOAMYLASE 3);
alpha-amylase/ isoamylase | chr4:5784099-5788839 FORWARD
Length = 765
Score = 62 bits (150), Expect = 5e-010
Identities = 44/140 (31%), Positives = 64/140 (45%), Gaps = 11/140 (7%)
Frame = +1
Query: 355 GSPELSSELSWNCGEEGATNKSAVLQTRLKQIRNFLFIQYVSLGIPVLNMGDECGVSTKG 534
G+ + SWNCG EG T + + R +Q++NF +S G P++ MGDE G + G
Sbjct: 560 GNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYG 619
Query: 535 SPLL----ESRKPFDWNMLASAFGSQITQFISCMTTVRARRGDVFQRRNFLKPENIVWHA 702
+ S F W L A +F S + R V + NFL I WH
Sbjct: 620 NNNSYGHDTSLNNFQWKEL-DAKKQNHFRFFSEVIKFR-HSHHVLKHENFLTQGEITWHE 677
Query: 703 NDQTAPKWEDSSSKFLALEI 762
++ W++S SKFLA +
Sbjct: 678 DN-----WDNSESKFLAFTL 692
Database: TAIR9 protein
Posted date: Wed Jul 08 15:16:08 2009
Number of letters in database: 13,468,323
Number of sequences in database: 33,410
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,924,865,478
Number of Sequences: 33410
Number of Extensions: 5924865478
Number of Successful Extensions: 216150274
Number of sequences better than 0.0: 0
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