BLASTX 7.6.2
Query= UN14422 /QuerySize=818
(817 letters)
Database: GenBank nr;
15,229,318 sequences; 5,219,829,378 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|18397066|ref|NP_565354.1| uncharacterized protein [Arabidopsi... 239 4e-061
gi|297835930|ref|XP_002885847.1| hypothetical protein ARALYDRAFT... 237 2e-060
gi|255583110|ref|XP_002532322.1| aldose 1-epimerase, putative [R... 171 2e-040
gi|35210494|dbj|BAC87890.1| aspartic acid-rich protein aspolin2 ... 127 2e-027
gi|35210492|dbj|BAC87889.1| aspartic acid-rich protein aspolin1 ... 127 2e-027
gi|108801632|ref|YP_641829.1| ATPase [Mycobacterium sp. MCS] 126 7e-027
gi|33284873|emb|CAE17619.1| novel protein (zgc:100957) [Danio re... 124 1e-026
gi|326666048|ref|XP_003198182.1| PREDICTED: hypothetical protein... 124 2e-026
gi|237874200|ref|NP_001003620.2| calsequestrin-like [Danio rerio] 123 3e-026
gi|307208951|gb|EFN86162.1| hypothetical protein EAI_13635 [Harp... 123 3e-026
gi|51968341|dbj|BAD42844.1| aspartic acid-rich protein aspolin2-... 122 6e-026
gi|51968339|dbj|BAD42843.1| aspartic acid-rich protein aspolin2-... 122 6e-026
gi|51968337|dbj|BAD42842.1| aspartic acid-rich protein aspolin1 ... 122 7e-026
gi|307211379|gb|EFN87506.1| Serine/threonine-protein kinase DCLK... 122 7e-026
>gi|18397066|ref|NP_565354.1| uncharacterized protein [Arabidopsis thaliana]
Length = 168
Score = 239 bits (609), Expect = 4e-061
Identities = 122/171 (71%), Positives = 146/171 (85%), Gaps = 10/171 (5%)
Frame = -3
Query: 710 EVVAARSMEETQRQQTWACSATETLLILLGRNLACLLLAAESLLIGLIAEDQRLSVKKGY 531
EVVA SM +TQRQ+TWACS TE+ L+L+G+NLACLLL AESLLIGL+A DQRLSVK+GY
Sbjct: 2 EVVAVGSM-KTQRQETWACSVTESFLLLVGKNLACLLLVAESLLIGLVAVDQRLSVKEGY 60
Query: 530 KKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFS---GDEGEEEADPEDDP 360
K+G EENKD SDSD DDDDDEDAD +DDDD+DDANDE+FS GDEGEEEADPEDDP
Sbjct: 61 KRGSRIEENKDASDSD--DDDDDEDAD-EDDDDEDDANDEDFSGGEGDEGEEEADPEDDP 117
Query: 359 APNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDEDVRQPPAK 207
NGG GSDD++DD D+E D+DD+D+D ++E+EDD +EEDD+DVRQPP+K
Sbjct: 118 VTNGGGGSDDEDDD--DEEGDNDDEDEDNEDEEEDD-DEEDDDDVRQPPSK 165
>gi|297835930|ref|XP_002885847.1| hypothetical protein ARALYDRAFT_899524
[Arabidopsis lyrata subsp. lyrata]
Length = 168
Score = 237 bits (604), Expect = 2e-060
Identities = 122/170 (71%), Positives = 146/170 (85%), Gaps = 8/170 (4%)
Frame = -3
Query: 710 EVVAARSMEETQRQQTWACSATETLLILLGRNLACLLLAAESLLIGLIAEDQRLSVKKGY 531
EVVA SM ETQRQ+TWACS TE+ LIL+G+NLACLLL AESLLIGLIA DQRLS+K+GY
Sbjct: 2 EVVAVGSM-ETQRQETWACSVTESFLILVGKNLACLLLVAESLLIGLIAVDQRLSLKEGY 60
Query: 530 KKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGE--EEADPEDDPA 357
K+G EENKD SD++D D+DDDEDAD +DDDD+DDANDE+FSG EG+ EEADPEDDP
Sbjct: 61 KRGSRAEENKDASDTED-DNDDDEDAD-EDDDDEDDANDEDFSGGEGDEGEEADPEDDPV 118
Query: 356 PNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDEDVRQPPAK 207
NGG GS DEDDD D+E D+DDDD+D +E++E+DE+EEDD+DVRQPP+K
Sbjct: 119 ANGGGGS--DEDDDDDEEGDNDDDDED-NEDEEEDEDEEDDDDVRQPPSK 165
>gi|255583110|ref|XP_002532322.1| aldose 1-epimerase, putative [Ricinus
communis]
Length = 174
Score = 171 bits (431), Expect = 2e-040
Identities = 95/182 (52%), Positives = 122/182 (67%), Gaps = 22/182 (12%)
Frame = -3
Query: 713 MEV--VAARSMEETQRQQTWACSATETLLILLG----RNLACLLLAAESLL------IGL 570
MEV V S+E T WACS E +++ +LA L A SLL L
Sbjct: 1 MEVGSVTNSSVERT----IWACSVVEAMVVETAVSAWESLARLFFVAGSLLNDINTSSKL 56
Query: 569 IAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEG 390
A D+R + + +K+ RPD ENKD SD++D+D+DDD+ D+ D DDDANDE+FSG+EG
Sbjct: 57 TAIDERFPLDELFKRKRPDAENKDASDTEDDDEDDDD----DNADPDDDANDEDFSGEEG 112
Query: 389 EEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDEDVRQPPA 210
EEEADPEDDP NG GSDD++DDD DD D D++DDDED EDE++EEEE++E+V QPPA
Sbjct: 113 EEEADPEDDPEANGEGGSDDEDDDDDDD--DGDEEDDDEDGEDEEEEEEEEEEEVPQPPA 170
Query: 209 KK 204
KK
Sbjct: 171 KK 172
>gi|35210494|dbj|BAC87890.1| aspartic acid-rich protein aspolin2 [Gadus
chalcogrammus]
Length = 347
Score = 127 bits (318), Expect = 2e-027
Identities = 57/134 (42%), Positives = 86/134 (64%)
Frame = -3
Query: 629 LLGRNLACLLLAAESLLIGLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDAD 450
LLG +A L L G+ A+D ++G D+++ D D DD+DDDDD+D D
Sbjct: 8 LLGLLVALLCSFQTPLAPGVKAQDLPFVQEEGLVADDDDDDDDDAGDDDDDDDDDDDDDD 67
Query: 449 ADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDED 270
DDDDDDDD +D+ D+ +++ D +DD + DDD+DDD DD+ D DDDDDD+D
Sbjct: 68 DDDDDDDDDDDDDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 127
Query: 269 EEDEDDEEEEDDED 228
++D+DD++++DD+D
Sbjct: 128 DDDDDDDDDDDDDD 141
Score = 125 bits (312), Expect = 1e-026
Identities = 49/96 (51%), Positives = 74/96 (77%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD + AG
Sbjct: 170 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDHEAGD 229
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 230 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 265
Score = 123 bits (307), Expect = 4e-026
Identities = 51/116 (43%), Positives = 78/116 (67%)
Frame = -3
Query: 575 GLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGD 396
GL+A+D D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D
Sbjct: 39 GLVADDDDDDDDDAGDDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDDDDDDDDD 98
Query: 395 EGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
+ +++ D +DD + DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 99 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 154
Score = 122 bits (304), Expect = 1e-025
Identities = 48/96 (50%), Positives = 72/96 (75%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D+E+ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 79 DDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 138
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 139 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 174
>gi|35210492|dbj|BAC87889.1| aspartic acid-rich protein aspolin1 [Gadus
chalcogrammus]
Length = 228
Score = 127 bits (318), Expect = 2e-027
Identities = 57/134 (42%), Positives = 86/134 (64%)
Frame = -3
Query: 629 LLGRNLACLLLAAESLLIGLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDAD 450
LLG +A L L G+ A+D ++G D+++ D D DD+DDDDD+D D
Sbjct: 8 LLGLLVALLCSFQTPLAPGVKAQDLPFVQEEGLVADDDDDDDDDAGDDDDDDDDDDDDDD 67
Query: 449 ADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDED 270
DDDDDDDD +D+ D+ +++ D +DD + DDD+DDD DD+ D DDDDDD+D
Sbjct: 68 DDDDDDDDDDDDDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 127
Query: 269 EEDEDDEEEEDDED 228
++D+DD++++DD+D
Sbjct: 128 DDDDDDDDDDDDDD 141
Score = 123 bits (307), Expect = 4e-026
Identities = 51/116 (43%), Positives = 78/116 (67%)
Frame = -3
Query: 575 GLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGD 396
GL+A+D D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D
Sbjct: 39 GLVADDDDDDDDDAGDDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDDDDDDDDD 98
Query: 395 EGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
+ +++ D +DD + DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 99 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 154
Score = 122 bits (304), Expect = 1e-025
Identities = 48/96 (50%), Positives = 72/96 (75%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D+E+ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 79 DDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 138
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 139 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 174
>gi|108801632|ref|YP_641829.1| ATPase [Mycobacterium sp. MCS]
Length = 385
Score = 126 bits (314), Expect = 7e-027
Identities = 49/102 (48%), Positives = 76/102 (74%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 208 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 267
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDEDVRQPPA 210
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D+ + PA
Sbjct: 268 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDLEEAPA 309
>gi|33284873|emb|CAE17619.1| novel protein (zgc:100957) [Danio rerio]
Length = 579
Score = 124 bits (311), Expect = 1e-026
Identities = 49/97 (50%), Positives = 73/97 (75%)
Frame = -3
Query: 518 PDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAG 339
PDE++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 375 PDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 434
Query: 338 SDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 435 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 471
Score = 122 bits (304), Expect = 1e-025
Identities = 48/96 (50%), Positives = 72/96 (75%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 484 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 543
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DDED
Sbjct: 544 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDED 579
>gi|326666048|ref|XP_003198182.1| PREDICTED: hypothetical protein LOC100007086
[Danio rerio]
Length = 293
Score = 124 bits (310), Expect = 2e-026
Identities = 58/137 (42%), Positives = 88/137 (64%), Gaps = 2/137 (1%)
Frame = -3
Query: 632 ILLGRNLACLLLAAESLL--IGLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDE 459
+LLG LA L L + A+D L ++G D+++ D D DD+DDDDD+
Sbjct: 7 LLLGLLLALLCSFQTPLAPSASVKAQDLPLIREEGLMADDDDDDDDDDDDDDDDDDDDDD 66
Query: 458 DADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDD 279
D D DDDDDDDD +D++ D+ +++ D +DD + DDD+DDD DD+ D DDDDD
Sbjct: 67 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 126
Query: 278 DEDEEDEDDEEEEDDED 228
D+D++D+DD++++DD+D
Sbjct: 127 DDDDDDDDDDDDDDDDD 143
>gi|237874200|ref|NP_001003620.2| calsequestrin-like [Danio rerio]
Length = 553
Score = 123 bits (308), Expect = 3e-026
Identities = 48/97 (49%), Positives = 73/97 (75%)
Frame = -3
Query: 518 PDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAG 339
PD+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 375 PDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 434
Query: 338 SDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 435 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 471
>gi|307208951|gb|EFN86162.1| hypothetical protein EAI_13635 [Harpegnathos
saltator]
Length = 208
Score = 123 bits (308), Expect = 3e-026
Identities = 50/106 (47%), Positives = 75/106 (70%)
Frame = -3
Query: 545 VKKGYKKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPED 366
V G G D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +D
Sbjct: 36 VSNGDGDGDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 95
Query: 365 DPAPNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
D + DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 96 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 141
>gi|51968341|dbj|BAD42844.1| aspartic acid-rich protein aspolin2-2 [Cyprinus
carpio]
Length = 282
Score = 122 bits (306), Expect = 6e-026
Identities = 57/137 (41%), Positives = 87/137 (63%), Gaps = 2/137 (1%)
Frame = -3
Query: 632 ILLGRNLACLLLAAESLL--IGLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDE 459
+LLG LA L L + A+D ++G D+++ D D DD+DDDDD+
Sbjct: 7 LLLGLLLALLCSFQTPLAPSASVKAQDLPFIREEGLMADDDDDDDDDDDDDDDDDDDDDD 66
Query: 458 DADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDD 279
D D DDDDDDDD +D++ D+ +++ D +DD + DDD+DDD DD+ D DDDDD
Sbjct: 67 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 126
Query: 278 DEDEEDEDDEEEEDDED 228
D+D++D+DD++++DD+D
Sbjct: 127 DDDDDDDDDDDDDDDDD 143
>gi|51968339|dbj|BAD42843.1| aspartic acid-rich protein aspolin2-1 [Cyprinus
carpio]
Length = 272
Score = 122 bits (306), Expect = 6e-026
Identities = 57/137 (41%), Positives = 87/137 (63%), Gaps = 2/137 (1%)
Frame = -3
Query: 632 ILLGRNLACLLLAAESLL--IGLIAEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDE 459
+LLG LA L L + A+D ++G D+++ D D DD+DDDDD+
Sbjct: 7 LLLGLLLALLCSFQTPLAPSASVKAQDLPFIREEGLMADDDDDDDDDDDDDDDDDDDDDD 66
Query: 458 DADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDD 279
D D DDDDDDDD +D++ D+ +++ D +DD + DDD+DDD DD+ D DDDDD
Sbjct: 67 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 126
Query: 278 DEDEEDEDDEEEEDDED 228
D+D++D+DD++++DD+D
Sbjct: 127 DDDDDDDDDDDDDDDDD 143
>gi|51968337|dbj|BAD42842.1| aspartic acid-rich protein aspolin1 [Cyprinus
carpio]
Length = 186
Score = 122 bits (305), Expect = 7e-026
Identities = 50/113 (44%), Positives = 78/113 (69%)
Frame = -3
Query: 566 AEDQRLSVKKGYKKGRPDEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGE 387
A+D ++G D+++ D D DD+DDDDD+D D DDDDDDDD +D++ D+ +
Sbjct: 31 AQDLPFIREEGLMADDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 90
Query: 386 EEADPEDDPAPNGGAGSDDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
++ D +DD + DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 91 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 143
>gi|307211379|gb|EFN87506.1| Serine/threonine-protein kinase DCLK2 [Harpegnathos
saltator]
Length = 1339
Score = 122 bits (305), Expect = 7e-026
Identities = 49/96 (51%), Positives = 71/96 (73%)
Frame = -3
Query: 515 DEENKDGSDSDDNDDDDDEDADADDDDDDDDANDENFSGDEGEEEADPEDDPAPNGGAGS 336
D EN D D DD+DDDDD+D D DDDDDDDD +D++ D+ +++ D +DD +
Sbjct: 697 DGENDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 756
Query: 335 DDDEDDDGDDESDSDDDDDDEDEEDEDDEEEEDDED 228
DDD+DDD DD+ D DDDDDD+D++D+DD++++DD+D
Sbjct: 757 DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 792
Database: GenBank nr
Posted date: Thu Sep 08 23:06:31 2011
Number of letters in database: 5,219,829,378
Number of sequences in database: 15,229,318
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,687,995,622,555
Number of Sequences: 15229318
Number of Extensions: 1687995622555
Number of Successful Extensions: 433925934
Number of sequences better than 0.0: 0
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