BLASTX 7.6.2
Query= UN16629 /QuerySize=1459
(1458 letters)
Database: TAIR9 protein;
33,410 sequences; 13,468,323 total letters
Score E
Sequences producing significant alignments: (bits) Value
TAIR9_protein||AT5G49980.1 | Symbols: AFB5 | AFB5 (Auxin F-Box p... 671 4e-193
TAIR9_protein||AT4G24390.2 | Symbols: | F-box family protein (F... 574 6e-164
TAIR9_protein||AT4G24390.1 | Symbols: | F-box family protein (F... 574 6e-164
TAIR9_protein||AT1G12820.1 | Symbols: AFB3 | AFB3 (AUXIN SIGNALI... 347 2e-095
TAIR9_protein||AT3G26810.1 | Symbols: AFB2 | AFB2 (AUXIN SIGNALI... 340 2e-093
TAIR9_protein||AT4G03190.1 | Symbols: GRH1, ATGRH1, AFB1 | GRH1 ... 331 1e-090
TAIR9_protein||AT3G62980.1 | Symbols: TIR1 | TIR1 (TRANSPORT INH... 321 1e-087
TAIR9_protein||AT2G39940.1 | Symbols: COI1 | COI1 (CORONATINE IN... 141 1e-033
>TAIR9_protein||AT5G49980.1 | Symbols: AFB5 | AFB5 (Auxin F-Box protein 5);
ubiquitin-protein ligase | chr5:20334420-20336531 REVERSE
Length = 620
Score = 671 bits (1730), Expect = 4e-193
Identities = 325/352 (92%), Positives = 337/352 (95%), Gaps = 2/352 (0%)
Frame = +2
Query: 5 EVHRLLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYL 184
E+HRLLLGAPQLTSLGTGSF HD E + EQEPDYA+AFRACKSVVCLSGFRELMPEYL
Sbjct: 271 ELHRLLLGAPQLTSLGTGSFSHD--EEPQSEQEPDYAAAFRACKSVVCLSGFRELMPEYL 328
Query: 185 PAILPVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELR 364
PAI PVCANLTSLNFSYANISPDMFKPII CHKLQVFWALDSICDEGLQAVAATCKELR
Sbjct: 329 PAIFPVCANLTSLNFSYANISPDMFKPIILNCHKLQVFWALDSICDEGLQAVAATCKELR 388
Query: 365 ELRIFPFDPREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVF 544
ELRIFPFDPREDSEGPVSELGLQA+SEGCRKLES+LYFCQRMTNAA+IAMS NCPELTVF
Sbjct: 389 ELRIFPFDPREDSEGPVSELGLQAISEGCRKLESILYFCQRMTNAAVIAMSENCPELTVF 448
Query: 545 RLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLS 724
RLCIMGRHRPDHVTGK MDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKL+RTLS
Sbjct: 449 RLCIMGRHRPDHVTGKPMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLVRTLS 508
Query: 725 VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGC 904
VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSAC LS GC
Sbjct: 509 VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACSLSKGC 568
Query: 905 CKDVARAMPNLVVEVIGSDDDDENREFVETLYMYRSLDGPRKDAPQFVTIL* 1060
CKD+ARAMPNLVVEVIGSDDDD+NR++VETLYMYRSLDGPR DAP+FVTIL*
Sbjct: 569 CKDIARAMPNLVVEVIGSDDDDDNRDYVETLYMYRSLDGPRNDAPKFVTIL* 620
>TAIR9_protein||AT4G24390.2 | Symbols: | F-box family protein (FBX14) |
chr4:12613909-12615966 REVERSE
Length = 624
Score = 574 bits (1478), Expect = 6e-164
Identities = 282/356 (79%), Positives = 315/356 (88%), Gaps = 6/356 (1%)
Frame = +2
Query: 5 EVHRLLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYL 184
E+HRL++ APQLTSLGTGSF DN + EQ+PDYA+AFRACKS+VCLSGFRE PEYL
Sbjct: 271 ELHRLMVRAPQLTSLGTGSFSPDNVP--QGEQQPDYAAAFRACKSIVCLSGFREFRPEYL 328
Query: 185 PAILPVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELR 364
AI VCANLTSLNFSYANISP M KPII CH ++VFWALDSI DEGLQAVAATCKELR
Sbjct: 329 LAISSVCANLTSLNFSYANISPHMLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELR 388
Query: 365 ELRIFPFDPREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVF 544
ELRIFPFDPREDSEGPVS +GLQA+SEGCRKLES+LYFCQ MTN A+ AMS NCP+LTVF
Sbjct: 389 ELRIFPFDPREDSEGPVSGVGLQAISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVF 448
Query: 545 RLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLS 724
RLCIMGRHRPDHVTGK MD+GFGAIVKNCKKLTRLAVSGLLTD+AF Y+GEYGKLIRTLS
Sbjct: 449 RLCIMGRHRPDHVTGKPMDDGFGAIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLS 508
Query: 725 VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGC 904
VAFAG+SD ALR+VLEGCP+LQKLEIRDSPFGDV LRSGMHRY NMRFVW+S+C +S G
Sbjct: 509 VAFAGNSDKALRYVLEGCPKLQKLEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGG 568
Query: 905 CKDVARAMPNLVVEVIGSD-DDDENR---EFVETLYMYRSLDGPRKDAPQFVTIL* 1060
C+ V+ A+PN+VVEV G+D DDDE+ ++VETLY+YRSLDGPRKDAP+FVTIL*
Sbjct: 569 CRGVSHALPNVVVEVFGADGDDDEDTVTGDYVETLYLYRSLDGPRKDAPKFVTIL* 624
>TAIR9_protein||AT4G24390.1 | Symbols: | F-box family protein (FBX14) |
chr4:12613909-12615966 REVERSE
Length = 624
Score = 574 bits (1478), Expect = 6e-164
Identities = 282/356 (79%), Positives = 315/356 (88%), Gaps = 6/356 (1%)
Frame = +2
Query: 5 EVHRLLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYL 184
E+HRL++ APQLTSLGTGSF DN + EQ+PDYA+AFRACKS+VCLSGFRE PEYL
Sbjct: 271 ELHRLMVRAPQLTSLGTGSFSPDNVP--QGEQQPDYAAAFRACKSIVCLSGFREFRPEYL 328
Query: 185 PAILPVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELR 364
AI VCANLTSLNFSYANISP M KPII CH ++VFWALDSI DEGLQAVAATCKELR
Sbjct: 329 LAISSVCANLTSLNFSYANISPHMLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELR 388
Query: 365 ELRIFPFDPREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVF 544
ELRIFPFDPREDSEGPVS +GLQA+SEGCRKLES+LYFCQ MTN A+ AMS NCP+LTVF
Sbjct: 389 ELRIFPFDPREDSEGPVSGVGLQAISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVF 448
Query: 545 RLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLS 724
RLCIMGRHRPDHVTGK MD+GFGAIVKNCKKLTRLAVSGLLTD+AF Y+GEYGKLIRTLS
Sbjct: 449 RLCIMGRHRPDHVTGKPMDDGFGAIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLS 508
Query: 725 VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGC 904
VAFAG+SD ALR+VLEGCP+LQKLEIRDSPFGDV LRSGMHRY NMRFVW+S+C +S G
Sbjct: 509 VAFAGNSDKALRYVLEGCPKLQKLEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGG 568
Query: 905 CKDVARAMPNLVVEVIGSD-DDDENR---EFVETLYMYRSLDGPRKDAPQFVTIL* 1060
C+ V+ A+PN+VVEV G+D DDDE+ ++VETLY+YRSLDGPRKDAP+FVTIL*
Sbjct: 569 CRGVSHALPNVVVEVFGADGDDDEDTVTGDYVETLYLYRSLDGPRKDAPKFVTIL* 624
>TAIR9_protein||AT1G12820.1 | Symbols: AFB3 | AFB3 (AUXIN SIGNALING F-BOX 3);
auxin binding / ubiquitin-protein ligase | chr1:4368879-4370780
REVERSE
Length = 578
Score = 347 bits (888), Expect = 2e-095
Identities = 180/359 (50%), Positives = 245/359 (68%), Gaps = 14/359 (3%)
Frame = +2
Query: 14 RLLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYLPAI 193
RL+ APQL LG GS+ ++E + E +A + S+ LSGF E+ P LPA
Sbjct: 224 RLMSCAPQLVDLGVGSY----ENEPDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAF 279
Query: 194 LPVCANLTSLNFSY-ANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELREL 370
P+C NL SLN SY A I + +I C +LQ W LDSI D+GL VAATCKEL+EL
Sbjct: 280 YPICQNLISLNLSYAAEIQGNHLIKLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQEL 339
Query: 371 RIFPFD--PREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVF 544
R+FP D ED+ V+E+GL A+S GC KL S+LYFC++MTNAALIA++ NCP F
Sbjct: 340 RVFPSDVHGEEDNNASVTEVGLVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRF 399
Query: 545 RLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLS 724
RLCI+ H+PDH+T +++DEGFGAIV+ CK L RL+VSGLLTDQ F Y+G Y + + LS
Sbjct: 400 RLCILEPHKPDHITFQSLDEGFGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLS 459
Query: 725 VAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGC 904
+AFAGD+D + +VL GC +++KLEIRDSPFG+ AL + + RY MR +WMS+C++++G
Sbjct: 460 IAFAGDTDKGMLYVLNGCKKMRKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGG 519
Query: 905 CKDVARAMPNLVVEVIGSDD-------DDENREFVETLYMYRSLDGPRKDAPQFVTIL* 1060
CK +A+ P L VE+I ++ +++ RE V+ LY+YR++ G RKDAP +V IL*
Sbjct: 520 CKRLAQNSPRLNVEIINENENNGMEQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL* 578
>TAIR9_protein||AT3G26810.1 | Symbols: AFB2 | AFB2 (AUXIN SIGNALING F-BOX 2);
auxin binding / ubiquitin-protein ligase | chr3:9868342-9870464
FORWARD
Length = 576
Score = 340 bits (870), Expect = 2e-093
Identities = 177/357 (49%), Positives = 235/357 (65%), Gaps = 12/357 (3%)
Frame = +2
Query: 14 RLLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYLPAI 193
RL+ APQ+ LG GS+++D SE + + + C S+ LSGF E P L A
Sbjct: 224 RLMACAPQIVDLGVGSYENDPDSESYLK----LMAVIKKCTSLRSLSGFLEAAPHCLSAF 279
Query: 194 LPVCANLTSLNFSY-ANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELREL 370
P+C NLTSLN SY A I +I C KLQ W LDSI D+GL+ VA+TCKEL+EL
Sbjct: 280 HPICHNLTSLNLSYAAEIHGSHLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQEL 339
Query: 371 RIFPFDPREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVFRL 550
R+FP D V+E GL A+S GC KL S+LYFCQ+MTNAAL+ ++ NCP FRL
Sbjct: 340 RVFPSDLLGGGNTAVTEEGLVAISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRL 399
Query: 551 CIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLSVA 730
CI+ ++PDHVT + +DEGFGAIVK CK L RL++SGLLTDQ F Y+G Y + LS+A
Sbjct: 400 CILEPNKPDHVTSQPLDEGFGAIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIA 459
Query: 731 FAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGCCK 910
FAGD+D + +VL GC +++KLEIRDSPFGD AL + + +Y MR +WMS+C++++ CK
Sbjct: 460 FAGDTDKGMLYVLNGCKKMKKLEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCK 519
Query: 911 DVARAMPNLVVEVIGSDDDD-------ENREFVETLYMYRSLDGPRKDAPQFVTIL* 1060
+A P L VE+I +D++ E R+ V+ LY+YR++ G R DAP FV IL*
Sbjct: 520 RLAEKAPWLNVEIINENDNNRMEENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL* 576
>TAIR9_protein||AT4G03190.1 | Symbols: GRH1, ATGRH1, AFB1 | GRH1 (GRR1-LIKE
PROTEIN 1); auxin binding / protein binding / ubiquitin-protein ligase
| chr4:1405108-1407057 REVERSE
Length = 586
Score = 331 bits (847), Expect = 1e-090
Identities = 172/347 (49%), Positives = 230/347 (66%), Gaps = 7/347 (2%)
Frame = +2
Query: 17 LLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYASAFRACKSVVCLSGFRELMPEYLPAIL 196
LL APQLT LGTGSF ++ + E + AF CK + LSG +++PEYLPA+
Sbjct: 226 LLRCAPQLTELGTGSF----AAQLKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALY 281
Query: 197 PVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELRELRI 376
VC LTSLN SYA + ++ C KLQ W +D I D+GL+AVA+ CKELRELR+
Sbjct: 282 SVCPGLTSLNLSYATVRMPDLVELLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRV 341
Query: 377 FPFDPREDSEG-PVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTVFRLC 553
FP +P D+ P++E GL VS+GCRKLESVLYFC + TNAAL ++ P L FRLC
Sbjct: 342 FPSEPDLDATNIPLTEQGLVFVSKGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLC 401
Query: 554 IMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTLSVAF 733
++ PD+ T + +D+GF AI + C+ L RL+VSGLL+D+AF+Y+G++ K +R LS+AF
Sbjct: 402 VIEPFAPDYKTNEPLDKGFKAIAEGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAF 461
Query: 734 AGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMGCCKD 913
AGDSD+ L H+L GC L+KLEIRD PFGD AL + MR +WMS+C +S G CK
Sbjct: 462 AGDSDLMLHHLLSGCESLKKLEIRDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKL 521
Query: 914 VARAMPNLVVEVIGSDDDDENREF--VETLYMYRSLDGPRKDAPQFV 1048
+++ MP L VEVI + E VE +Y+YR++ GPR D P+FV
Sbjct: 522 LSQKMPRLNVEVIDEHPPESRPESSPVERIYIYRTVAGPRMDTPEFV 568
>TAIR9_protein||AT3G62980.1 | Symbols: TIR1 | TIR1 (TRANSPORT INHIBITOR RESPONSE
1); auxin binding / protein binding / ubiquitin-protein ligase |
chr3:23273479-23276181 REVERSE
Length = 595
Score = 321 bits (821), Expect = 1e-087
Identities = 174/352 (49%), Positives = 227/352 (64%), Gaps = 16/352 (4%)
Frame = +2
Query: 17 LLLGAPQLTSLGTGSFKHDNQSEREQEQEPDYAS----AFRACKSVVCLSGFRELMPEYL 184
LL APQL LGTG + E PD S A CK + CLSGF + +P YL
Sbjct: 230 LLQRAPQLEELGTGGY--------TAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYL 281
Query: 185 PAILPVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICDEGLQAVAATCKELR 364
PA+ VC+ LT+LN SYA + ++ C KLQ W LD I D GL+ +A+TCK+LR
Sbjct: 282 PAVYSVCSRLTTLNLSYATVQSYDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLR 341
Query: 365 ELRIFPFDP-REDSEGPVSELGLQAVSEGCRKLESVLYFCQRMTNAALIAMSHNCPELTV 541
ELR+FP +P + ++E GL +VS GC KLESVLYFC++MTNAALI ++ N P +T
Sbjct: 342 ELRVFPSEPFVMEPNVALTEQGLVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTR 401
Query: 542 FRLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLIRTL 721
FRLCI+ PD++T + +D GFGAIV++CK L RL++SGLLTD+ F Y+G Y K + L
Sbjct: 402 FRLCIIEPKAPDYLTLEPLDIGFGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEML 461
Query: 722 SVAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACKLSMG 901
SVAFAGDSD+ + HVL GC L+KLEIRD PFGD AL + + MR +WMS+C +S G
Sbjct: 462 SVAFAGDSDLGMHHVLSGCDSLRKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFG 521
Query: 902 CCKDVARAMPNLVVEVI---GSDDDDENREFVETLYMYRSLDGPRKDAPQFV 1048
CK + + MP L VEVI G+ D VE +++YR++ GPR D P FV
Sbjct: 522 ACKLLGQKMPKLNVEVIDERGAPDSRPESCPVERVFIYRTVAGPRFDMPGFV 573
>TAIR9_protein||AT2G39940.1 | Symbols: COI1 | COI1 (CORONATINE INSENSITIVE 1);
protein binding / ubiquitin-protein ligase | chr2:16672848-16675486
REVERSE
Length = 593
Score = 141 bits (355), Expect = 1e-033
Identities = 86/278 (30%), Positives = 143/278 (51%), Gaps = 8/278 (2%)
Frame = +2
Query: 143 VCLSGFRELMPEYLPAILPVCANLTSLNFSYANISPDMFKPIIHTCHKLQVFWALDSICD 322
+C G + P +P + P A + L+ YA + + +I C L+V + I D
Sbjct: 272 LCRLGLSYMGPNEMPILFPFAAQIRKLDLLYALLETEDHCTLIQKCPNLEVLETRNVIGD 331
Query: 323 EGLQAVAATCKELRELRI---FPFDPREDSEGPVSELGLQAVSEGCRKLESVLYFCQRMT 493
GL+ +A CK+L+ LRI ED EG VS+ GL A+++GC++LE + + +T
Sbjct: 332 RGLEVLAQYCKQLKRLRIERGADEQGMEDEEGLVSQRGLIALAQGCQELEYMAVYVSDIT 391
Query: 494 NAALIAMSHNCPELTVFRLCIMGRHRPDHVTGKAMDEGFGAIVKNCKKLTRLAV---SGL 664
N +L ++ L FRL ++ R + +T +D G +++ CKKL R A G
Sbjct: 392 NESLESIGTYLKNLCDFRLVLLDRE--ERITDLPLDNGVRSLLIGCKKLRRFAFYLRQGG 449
Query: 665 LTDQAFRYMGEYGKLIRTLSVAFAGDSDMALRHVLEGCPRLQKLEIRDSPFGDVALRSGM 844
LTD Y+G+Y +R + + + G+SD L GCP LQKLE+R F + A+ + +
Sbjct: 450 LTDLGLSYIGQYSPNVRWMLLGYVGESDEGLMEFSRGCPNLQKLEMRGCCFSERAIAAAV 509
Query: 845 HRYYNMRFVWMSACKLSMGCCKDVARAMPNLVVEVIGS 958
+ ++R++W+ + SM + A P +E+I S
Sbjct: 510 TKLPSLRYLWVQGYRASMTGQDLMQMARPYWNIELIPS 547
Database: TAIR9 protein
Posted date: Wed Jul 08 15:16:08 2009
Number of letters in database: 13,468,323
Number of sequences in database: 33,410
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,775,572,671
Number of Sequences: 33410
Number of Extensions: 8775572671
Number of Successful Extensions: 306414901
Number of sequences better than 0.0: 0
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