BLASTX 7.6.2
Query= UN16635 /QuerySize=979
(978 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28891|S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 84 2e-015
sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 80 2e-014
sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 80 3e-014
sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Ra... 80 3e-014
sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 79 6e-014
sp|Q99N99|S5A2_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 77 1e-013
sp|Q28892|S5A2_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 76 3e-013
sp|P31214|S5A2_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Ra... 75 5e-013
sp|O18765|S5A2_PIG 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Su... 75 6e-013
sp|P31213|S5A2_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 73 3e-012
sp|P40526|DFG10_YEAST Protein DFG10 OS=Saccharomyces cerevisiae ... 62 7e-009
sp|Q8AVI9|S5A3_XENLA 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=... 57 2e-007
>sp|Q28891|S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca
fascicularis GN=SRD5A1 PE=1 SV=1
Length = 263
Score = 84 bits (205), Expect = 2e-015
Identities = 48/142 (33%), Positives = 79/142 (55%), Gaps = 5/142 (3%)
Frame = +1
Query: 430 FIITSSYFSSTVLMLYSQSFTLGLTEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEE 609
F + Y S L ++ +T+P F LIG +++ G++ N++ +L LR +
Sbjct: 127 FCTFNGYLQSRYLSHWAVYADDWVTDPRF---LIGFGLWLAGMLINIHSDHILRNLR--K 181
Query: 610 GGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATR 789
G YKIP+GGLF+ + +Y EI+ + + + S ++ +FA T +L GR+
Sbjct: 182 PGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHH 241
Query: 790 RWYLSKFDDFPKHVKALIPFVF 855
RWYL KF+++PK K LIPF+F
Sbjct: 242 RWYLQKFEEYPKFRKILIPFLF 263
>sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus
GN=Srd5a1 PE=2 SV=2
Length = 255
Score = 80 bits (196), Expect = 2e-014
Identities = 47/141 (33%), Positives = 76/141 (53%), Gaps = 5/141 (3%)
Frame = +1
Query: 430 FIITSSYFSSTVLMLYSQSFTLGLTEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEE 609
F + Y S L ++ +T P F L G +++VG+V N++ +L LR +
Sbjct: 119 FCTLNGYLQSRYLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLR--K 173
Query: 610 GGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATR 789
G+ YKIP+GGLF+ + +Y E++ + F + S ++ FA+ T L R+
Sbjct: 174 PGETGYKIPRGGLFEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHH 233
Query: 790 RWYLSKFDDFPKHVKALIPFV 852
+WYL KF+D+PK K LIPF+
Sbjct: 234 QWYLEKFEDYPKTRKILIPFL 254
>sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens
GN=SRD5A1 PE=1 SV=1
Length = 259
Score = 80 bits (195), Expect = 3e-014
Identities = 42/119 (35%), Positives = 71/119 (59%), Gaps = 5/119 (4%)
Frame = +1
Query: 499 LTEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYL 678
+T+P F LIG +++ G++ N++ +L LR + G YKIP+GGLF+ + +Y
Sbjct: 146 VTDPRF---LIGFGLWLTGMLINIHSDHILRNLR--KPGDTGYKIPRGGLFEYVTAANYF 200
Query: 679 FEIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
EI+ + + + S ++ +FA T +L GR+ WYL KF+++PK K +IPF+F
Sbjct: 201 GEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259
>sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus
GN=Srd5a1 PE=1 SV=2
Length = 259
Score = 80 bits (195), Expect = 3e-014
Identities = 55/181 (30%), Positives = 95/181 (52%), Gaps = 16/181 (8%)
Frame = +1
Query: 334 LLKSALALHFLKR--IFEVLCIHKYSGEMAVDSAFIITSSY--FSSTVLMLYSQSFTL-- 495
+L + +H+++R +F VL G+ + F++ + F+ V Y F +
Sbjct: 86 VLLAMFLIHYVQRTLVFPVLI---RGGKPTLLVTFVLAFLFCTFNGYVQSRYLSQFAVYA 142
Query: 496 --GLTEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICP 669
+T P F L G +++VG+V N++ +L LR + G+ YKIP+GGLF+ +
Sbjct: 143 EDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLR--KPGETGYKIPRGGLFEYVSAA 197
Query: 670 HYLFEIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPF 849
+Y E++ + F + S ++ FA+ T L+ R+ +WY KF+D+PK K LIPF
Sbjct: 198 NYFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPF 257
Query: 850 V 852
V
Sbjct: 258 V 258
>sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus
GN=SRD5A1 PE=2 SV=1
Length = 257
Score = 79 bits (192), Expect = 6e-014
Identities = 45/141 (31%), Positives = 78/141 (55%), Gaps = 5/141 (3%)
Frame = +1
Query: 430 FIITSSYFSSTVLMLYSQSFTLGLTEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEE 609
F + Y S L Y+ L++P F L G ++++G++ N++ +L LR +
Sbjct: 121 FCTYNGYLQSRYLSQYAVYADDWLSDPRF---LTGSALWLIGMLINIHSDHVLRNLR--K 175
Query: 610 GGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATR 789
G+ YKIP+GGLF+ I +Y E++ + + + S +I ++FA+ T L R+
Sbjct: 176 PGETGYKIPRGGLFEYISAANYFGEVVEWCGYALASWSIQGWAFAVFTFCVLFTRAQQHH 235
Query: 790 RWYLSKFDDFPKHVKALIPFV 852
+WY KF+D+PK K +IPF+
Sbjct: 236 KWYHEKFEDYPKFRKIMIPFL 256
>sp|Q99N99|S5A2_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Mus musculus
GN=Srd5a2 PE=2 SV=1
Length = 254
Score = 77 bits (189), Expect = 1e-013
Identities = 38/109 (34%), Positives = 70/109 (64%), Gaps = 2/109 (1%)
Frame = +1
Query: 529 IGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFF 708
+G+ F++G+ N++ +L +LR + G+ +Y+IP+GGLF + ++L EII + +
Sbjct: 148 VGVFFFILGMGINIHSDCMLRQLR--KPGEVIYRIPQGGLFTYVSGANFLGEIIEWMGYA 205
Query: 709 MVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
+ + ++ +F+FA T +L +++ R+YL F D+PK KALIPF+F
Sbjct: 206 LATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF 254
>sp|Q28892|S5A2_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca
fascicularis GN=SRD5A2 PE=1 SV=1
Length = 254
Score = 76 bits (186), Expect = 3e-013
Identities = 48/178 (26%), Positives = 93/178 (52%), Gaps = 9/178 (5%)
Frame = +1
Query: 334 LLKSALALHFLKRIFEVLCIHK---YSGEMAVDSAFIITSSYFSSTVLMLYSQSFTLG-L 501
+L +H+ R F +++ Y + + F + ++Y + G
Sbjct: 82 VLLGLFCVHYFHRTFVYSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGWY 141
Query: 502 TEPAFDTKLIGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLF 681
T+ F +G+ +F++G+ N++ +L +LR + G+ Y+IPKGGLF + ++L
Sbjct: 142 TDIRF---CLGVFLFILGMGVNIHSDYILRQLR--KPGEITYRIPKGGLFTYVSGANFLG 196
Query: 682 EIIVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
EII + + + + ++ + +FA + +L R++ R+YL F+D+PK KALIPF+F
Sbjct: 197 EIIEWIGYALATWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254
>sp|P31214|S5A2_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Rattus norvegicus
GN=Srd5a2 PE=1 SV=1
Length = 254
Score = 75 bits (184), Expect = 5e-013
Identities = 38/108 (35%), Positives = 69/108 (63%), Gaps = 2/108 (1%)
Frame = +1
Query: 532 GIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFFM 711
G+ +F++G+ N++ L +LR + G+ +Y+IP+GGLF + ++L EII + + +
Sbjct: 149 GVFLFILGMGINIHSDYTLRQLR--KPGEVIYRIPRGGLFTYVSGANFLGEIIEWIGYAL 206
Query: 712 VSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
+ ++ +F+FA T +L +++ R+YL F D+PK KALIPF+F
Sbjct: 207 ATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF 254
>sp|O18765|S5A2_PIG 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa
GN=SRD5A2 PE=2 SV=2
Length = 254
Score = 75 bits (183), Expect = 6e-013
Identities = 37/109 (33%), Positives = 71/109 (65%), Gaps = 2/109 (1%)
Frame = +1
Query: 529 IGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFF 708
+G+ +F++G+ N++ +L +LR + G+ +YKIP+GGLF + ++L EII + +
Sbjct: 148 LGVFLFILGMGINIHSDYILRQLR--KPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYA 205
Query: 709 MVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
+ + ++ + +FA + +L R++ R+Y+ F+D+PK KALIPF+F
Sbjct: 206 LATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254
>sp|P31213|S5A2_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens
GN=SRD5A2 PE=1 SV=1
Length = 254
Score = 73 bits (177), Expect = 3e-012
Identities = 37/109 (33%), Positives = 70/109 (64%), Gaps = 2/109 (1%)
Frame = +1
Query: 529 IGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLFEIIVFWSFF 708
+G+ +F++G+ N++ +L +LR + G+ Y+IP+GGLF + ++L EII + +
Sbjct: 148 LGVFLFILGMGINIHSDYILRQLR--KPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYA 205
Query: 709 MVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFVF 855
+ + ++ + +FA + +L R++ R+YL F+D+PK KALIPF+F
Sbjct: 206 LATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254
>sp|P40526|DFG10_YEAST Protein DFG10 OS=Saccharomyces cerevisiae GN=DFG10 PE=2
SV=1
Length = 253
Score = 62 bits (148), Expect = 7e-009
Identities = 47/170 (27%), Positives = 76/170 (44%), Gaps = 21/170 (12%)
Frame = +1
Query: 358 HFLKRIFEVLCIHKYSGEMAVDSAFIITSSYFSSTVLM-----LYSQSFTLGLTEPAFDT 522
H L+R++E L + Y+ ++ + + +F S +L+ LY S L AF
Sbjct: 99 HSLRRLYETLYVLHYTSNSRMNWSHYLVGIWFYSVLLLILNISLYKNSIPNTLNMNAF-- 156
Query: 523 KLIGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKVYKIPKGGLFDTIICPHYLFEIIVFWS 702
++F + +HV+LA L Y +P G LF + CPHYL EII++ +
Sbjct: 157 -----IIFCIASWDQYKNHVILANLVK-------YSLPTGRLFRLVCCPHYLDEIIIYST 204
Query: 703 FFMVSQTIYSFSFAMGTTFYLVGRSYATRRWYLSKFDDFPKHVKALIPFV 852
Q Y + T+ L + T+ +Y KF D A+IPF+
Sbjct: 205 LLPYEQEFYLTLVWVITS--LTISALETKNYYRHKFKDNHVAPYAIIPFI 252
>sp|Q8AVI9|S5A3_XENLA 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=Xenopus laevis
GN=srd5a3 PE=2 SV=1
Length = 319
Score = 57 bits (136), Expect = 2e-007
Identities = 31/116 (26%), Positives = 59/116 (50%), Gaps = 7/116 (6%)
Frame = +1
Query: 526 LIGIVMFVVGIVGNLYHHVLLAKLRNEEGGKKV---YKIPKGGLFDTIICPHYLFEIIVF 696
++G+ ++V + H +LA LR G + + +P G F+ + CPHY E++++
Sbjct: 205 ILGLTLYVWASLHQYTCHCILADLRKSASGAIINLKHAVPTGDWFEKVSCPHYFAELLIY 264
Query: 697 WSFFMVSQTIYSFSFAMGTTFYLVGRSYAT---RRWYLSKFDDFPKHVKALIPFVF 855
S +V + + + + + L+ ++ A +Y KFD +P H KA IP +F
Sbjct: 265 LSIAVVFGLLNTIWWLV-VLYVLLSQALAAVLCHEFYHEKFDSYPIHRKAFIPLIF 319
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,472,851,095
Number of Sequences: 518415
Number of Extensions: 69472851095
Number of Successful Extensions: 496854919
Number of sequences better than 0.0: 0
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