BLASTX 7.6.2
Query= UN16717 /QuerySize=1129
(1128 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q03529|SCS7_YEAST Ceramide very long chain fatty acid hydroxy... 185 5e-046
sp|O13846|SCS7_SCHPO Ceramide very long chain fatty acid hydroxy... 164 1e-039
sp|Q2LAM0|FA2H_RAT Fatty acid 2-hydroxylase OS=Rattus norvegicus... 139 4e-032
sp|Q4R4P4|FA2H_MACFA Fatty acid 2-hydroxylase OS=Macaca fascicul... 138 1e-031
sp|Q7L5A8|FA2H_HUMAN Fatty acid 2-hydroxylase OS=Homo sapiens GN... 137 2e-031
sp|Q5MPP0|FA2H_MOUSE Fatty acid 2-hydroxylase OS=Mus musculus GN... 134 1e-030
>sp|Q03529|SCS7_YEAST Ceramide very long chain fatty acid hydroxylase SCS7
OS=Saccharomyces cerevisiae GN=SCS7 PE=1 SV=1
Length = 384
Score = 185 bits (469), Expect = 5e-046
Identities = 100/236 (42%), Positives = 132/236 (55%), Gaps = 8/236 (3%)
Frame = -3
Query: 892 VDLDKPLVFQV---GHLGEEYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVWWAIPTIWLP 722
+DL++PL+ Q+ + Y + IH+P +G +F E LT+T WW +P WLP
Sbjct: 148 LDLNRPLLMQILRSDFKKDFYVDQIHRPRHYGKGSAPLFGNFLEPLTKTAWWVVPVAWLP 207
Query: 721 VVCYVLSISAARGLTFPQMGLIVAFGVFTWTLLEYTLHRFLFHIE---TKSYWANTAHYL 551
VV Y + + A + + + GVF WTL+EY LHRFLFH + +S A H+L
Sbjct: 208 VVVYHMGV-ALKNMNQLFACFLFCVGVFVWTLIEYGLHRFLFHFDDWLPESNIAFATHFL 266
Query: 550 LHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTH 371
LHGCHH P D RLV PPT IL P +KL+ + A A G LFGYV YD H
Sbjct: 267 LHGCHHYLPMDKYRLVMPPTLFVILCAPFYKLVFALLPLYWAYAGFAGGLFGYVCYDECH 326
Query: 370 FYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDRVFGTLPGTKAAQKKL 203
F+LHH + P + LKKYHL HH++ G+G+TS WD VFGT G A K+
Sbjct: 327 FFLHHSK-LPPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSKM 381
>sp|O13846|SCS7_SCHPO Ceramide very long chain fatty acid hydroxylase-like
protein C19G12.08 OS=Schizosaccharomyces pombe GN=SPAC19G12.08 PE=2
SV=1
Length = 347
Score = 164 bits (414), Expect = 1e-039
Identities = 90/228 (39%), Positives = 124/228 (54%), Gaps = 12/228 (5%)
Frame = -3
Query: 892 VDLDKPLVFQV--GHLGEE-YQEWIHQP-IVCVEGPRFFQSDFWEFLTRTVWWAIPTIWL 725
+DL KPL+ Q+ G++ ++ Y + +H+P G +F E LT+T W+ IP IW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDVYLDQVHRPRHYRGSGSAPLFGNFLEPLTKTPWYMIPLIWV 166
Query: 724 PVVCYVLSISAARGLTFPQMGLIVAFGVFTWTLLEYTLHRFLFHIETKSYWAN-----TA 560
P V Y + A G+ F G+FTWTL+EYT+HRFLFH++ Y + T
Sbjct: 167 PCVTYGF-LYACTGIPFSVAITFFIIGLFTWTLVEYTMHRFLFHLD--EYTPDHPIFLTM 223
Query: 559 HYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYD 380
H+ HGCHH P D RLV PP I P + + L+ A G + GYV YD
Sbjct: 224 HFAFHGCHHFLPADKYRLVMPPALFLIFATPWYHFIQLVLPHYIGVAGFSGAILGYVFYD 283
Query: 379 CTHFYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDRVFGT 236
TH++LHH + LK +HL+HH++ YGITS WDRVFGT
Sbjct: 284 LTHYFLHHRRMPNAYLTDLKTWHLDHHYKDYKSAYGITSWFWDRVFGT 331
>sp|Q2LAM0|FA2H_RAT Fatty acid 2-hydroxylase OS=Rattus norvegicus GN=Fa2h PE=1
SV=2
Length = 372
Score = 139 bits (349), Expect = 4e-032
Identities = 67/156 (42%), Positives = 98/156 (62%), Gaps = 4/156 (2%)
Frame = -3
Query: 667 MGLIVAFGVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLRLVFPP 494
+GL V G+ WTL+EY +HRFLFH++ + S++ H+++HG HHK P DG RLVFPP
Sbjct: 216 IGLFV-LGMLIWTLVEYLIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPP 274
Query: 493 TATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKK 317
++++ + L LI A A + G L GYV+YD TH+YLH G P + ++ + +K
Sbjct: 275 VPASVVVAFFYVFLRLILPEAVAGILFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKA 334
Query: 316 YHLNHHFRIQDKGYGITSSLWDRVFGTLPGTKAAQK 209
+H+ HHF Q G+GI++ LWD F TL +A K
Sbjct: 335 HHVKHHFEYQKSGFGISTKLWDYFFHTLIPEEADPK 370
Score = 80 bits (197), Expect = 2e-014
Identities = 40/82 (48%), Positives = 54/82 (65%), Gaps = 3/82 (3%)
Frame = -3
Query: 892 VDLDKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVWWAIPTIWLPVVC 713
VD KPL++QVGHLGE+Y EW+HQP+ R F SD E ++TVW+++P IW+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--ARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 181
Query: 712 YVLSISAARGLTFPQMGLIVAF 647
Y LS S R LT + L +F
Sbjct: 182 Y-LSWSYYRTLTQDNIRLFASF 202
>sp|Q4R4P4|FA2H_MACFA Fatty acid 2-hydroxylase OS=Macaca fascicularis GN=FA2H
PE=2 SV=1
Length = 372
Score = 138 bits (346), Expect = 1e-031
Identities = 63/141 (44%), Positives = 90/141 (63%), Gaps = 3/141 (2%)
Frame = -3
Query: 646 GVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLRLVFPPTATAILL 473
G+F W+L+EY +HRFLFH++ + SY+ H+++HG HHK P DG RLVFPP ++++
Sbjct: 222 GIFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Query: 472 VPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKKYHLNHHF 296
+ L LI A + G L GYV+YD TH+YLH G P ++ + LK +H+ HHF
Sbjct: 282 GVFYLCLQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHRGSYLYNLKAHHVKHHF 341
Query: 295 RIQDKGYGITSSLWDRVFGTL 233
Q G+GI++ LWD F TL
Sbjct: 342 AHQKSGFGISTKLWDYCFHTL 362
Score = 79 bits (192), Expect = 7e-014
Identities = 44/86 (51%), Positives = 57/86 (66%), Gaps = 6/86 (6%)
Frame = -3
Query: 892 VDLDKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVWWAIPTIWLPVVC 713
VD KPL++QVGHLGE+Y EW+HQP+ R F SD E L++TVW+++P IW+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--TRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVL 181
Query: 712 YVLSISAARGLTFPQMGLIVAFGVFT 635
Y LS S R TF Q G + F FT
Sbjct: 182 Y-LSWSYYR--TFAQ-GNVRLFTSFT 203
>sp|Q7L5A8|FA2H_HUMAN Fatty acid 2-hydroxylase OS=Homo sapiens GN=FA2H PE=1
SV=1
Length = 372
Score = 137 bits (343), Expect = 2e-031
Identities = 63/145 (43%), Positives = 91/145 (62%), Gaps = 3/145 (2%)
Frame = -3
Query: 646 GVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLRLVFPPTATAILL 473
G F W+L+EY +HRFLFH++ + SY+ H+++HG HHK P DG RLVFPP ++++
Sbjct: 222 GTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Query: 472 VPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKKYHLNHHF 296
+ + LI A + G L GYV+YD TH+YLH G P + ++ + LK +H+ HHF
Sbjct: 282 GVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHF 341
Query: 295 RIQDKGYGITSSLWDRVFGTLPGTK 221
Q G+GI++ LWD F TL K
Sbjct: 342 AHQKSGFGISTKLWDYCFHTLTPEK 366
Score = 82 bits (200), Expect = 8e-015
Identities = 50/132 (37%), Positives = 72/132 (54%), Gaps = 7/132 (5%)
Frame = -3
Query: 892 VDLDKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVWWAIPTIWLPVVC 713
VD KPL++QVGHLGE+Y EW+HQP+ R F SD E L++TVW+++P IW+P+V
Sbjct: 124 VDWRKPLLWQVGHLGEKYDEWVHQPV--TRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVL 181
Query: 712 YVLSISAARGLTFPQMGLIVAFGVFTWTLLEYTLHRFLFHIETKSYWANTAHYLLHG--C 539
Y LS S R + L +F + ++ LF + T + + YL+H
Sbjct: 182 Y-LSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGT--FLWSLIEYLIHRFLF 238
Query: 538 HHKHPQDGLRLV 503
H K P D L+
Sbjct: 239 HMKPPSDSYYLI 250
>sp|Q5MPP0|FA2H_MOUSE Fatty acid 2-hydroxylase OS=Mus musculus GN=Fa2h PE=1
SV=1
Length = 372
Score = 134 bits (336), Expect = 1e-030
Identities = 64/156 (41%), Positives = 95/156 (60%), Gaps = 4/156 (2%)
Frame = -3
Query: 667 MGLIVAFGVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLRLVFPP 494
+GL V G+ WT +EY +HRFLFH++ + S++ H+++HG HHK P DG RLVFPP
Sbjct: 216 IGLFV-LGMLFWTFVEYVIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPP 274
Query: 493 TATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKK 317
++++ + L LI + G L GYV+YD TH+YLH G P + ++ + +K
Sbjct: 275 VPASLVIAFFYVFLRLILPETVGGIIFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKA 334
Query: 316 YHLNHHFRIQDKGYGITSSLWDRVFGTLPGTKAAQK 209
+H+ HHF Q G+GI++ LWD F TL +A K
Sbjct: 335 HHVKHHFEYQKSGFGISTKLWDYFFHTLIPEEAHPK 370
Score = 78 bits (191), Expect = 9e-014
Identities = 38/72 (52%), Positives = 50/72 (69%), Gaps = 3/72 (4%)
Frame = -3
Query: 892 VDLDKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVWWAIPTIWLPVVC 713
VD KPL++QVGHLGE+Y EW+HQP+ R F SD E ++TVW+++P IW+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--ARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 181
Query: 712 YVLSISAARGLT 677
Y LS S R LT
Sbjct: 182 Y-LSWSYYRTLT 192
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,472,851,095
Number of Sequences: 518415
Number of Extensions: 69472851095
Number of Successful Extensions: 496854919
Number of sequences better than 0.0: 0
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