BLASTX 7.6.2
Query= UN17158 /QuerySize=1322
(1321 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32962|NRL2_ARATH Nitrilase 2 OS=Arabidopsis thaliana GN=NIT2... 556 1e-157
sp|P32961|NRL1_ARATH Nitrilase 1 OS=Arabidopsis thaliana GN=NIT1... 550 1e-155
sp|P46010|NRL3_ARATH Nitrilase 3 OS=Arabidopsis thaliana GN=NIT3... 532 2e-150
sp|P46011|NRL4_ARATH Bifunctional nitrilase/nitrile hydratase NI... 442 3e-123
sp|Q42966|NRL4B_TOBAC Bifunctional nitrilase/nitrile hydratase N... 441 9e-123
sp|Q42965|NRL4A_TOBAC Bifunctional nitrilase/nitrile hydratase N... 440 1e-122
sp|Q6H849|NRL4_ORYSJ Bifunctional nitrilase/nitrile hydratase NI... 429 3e-119
sp|P40447|NIT1_YEAST Putative nitrilase-like protein NIT1 OS=Sac... 179 5e-044
sp|P82605|NRL_BACSX Nitrilase OS=Bacillus sp. (strain OxB-1) GN=... 162 6e-039
sp|Q02068|NRL1_RHORH Aliphatic nitrilase OS=Rhodococcus rhodochr... 151 2e-035
sp|P10045|NRLB_KLEPO Nitrilase, bromoxynil-specific OS=Klebsiell... 139 5e-032
sp|P40446|YIQ5_YEAST Putative nitrilase-like protein YIL165C OS=... 74 2e-012
sp|P20960|NRLA_ALCFA Nitrilase, arylacetone-specific OS=Alcalige... 62 6e-009
sp|Q03217|NRL2_RHORH Aliphatic nitrilase OS=Rhodococcus rhodochr... 62 8e-009
>sp|P32962|NRL2_ARATH Nitrilase 2 OS=Arabidopsis thaliana GN=NIT2 PE=1 SV=1
Length = 339
Score = 556 bits (1432), Expect = 1e-157
Identities = 267/335 (79%), Positives = 298/335 (88%), Gaps = 4/335 (1%)
Frame = -1
Query: 1243 TPTFSDMPSTIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPR 1064
TP STIVRATIVQASTVYNDTP T+EKA +FI +AAS G++LV+FPE +IGGYPR
Sbjct: 7 TPFNGVASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPR 66
Query: 1063 GYRFGIAVGVHNEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTL 884
G+RFG+ VGVHNE+G D FRKYHASAI VPGPEV+KLAE+A K+ VYLVMGAIEKDGYTL
Sbjct: 67 GFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTL 126
Query: 883 YCTALFFSSEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPL 704
YCTALFFS +G+FLGKHRK+MPTSLERCIWG GDGSTIPVYDTP+GKLGAAICWENRMPL
Sbjct: 127 YCTALFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPL 186
Query: 703 FRTALYGKGIELYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFT 524
+RTALY KGIELYCAPTADG+K WQSSM+HIAIEGGCFVLSACQF LRKDFPDH DYLFT
Sbjct: 187 YRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFT 246
Query: 523 DPSSGMYDGEHQDTIVSQGGSVIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDV 344
D YD + D+IVSQGGSVIISPLG++LAGPNFESEGLITADLDLGD+ARAKLYFD
Sbjct: 247 D----WYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDS 302
Query: 343 VGHYSRPEIFNLTVNENQKKPVTYVSKSVKAADDS 239
VGHYSRP++ +LTVNE+ KKPVT++SK KA DDS
Sbjct: 303 VGHYSRPDVLHLTVNEHPKKPVTFISKVEKAEDDS 337
>sp|P32961|NRL1_ARATH Nitrilase 1 OS=Arabidopsis thaliana GN=NIT1 PE=1 SV=2
Length = 346
Score = 550 bits (1416), Expect = 1e-155
Identities = 266/348 (76%), Positives = 304/348 (87%), Gaps = 5/348 (1%)
Frame = -1
Query: 1282 SSTEDMSKALKGTTPTFSDMPSTIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQL 1103
SST+DMS ++ TP PST VR TIVQ+STVYNDTP TI+KA ++I +AAS GA+L
Sbjct: 2 SSTKDMS-TVQNATPFNGVAPSTTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAEL 60
Query: 1102 VLFPEGYIGGYPRGYRFGIAVGVHNEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVY 923
VLFPEG+IGGYPRG+RFG+AVGVHNE+G D FRKYHASAI VPGPEV +LA++ARK+ VY
Sbjct: 61 VLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVY 120
Query: 922 LVMGAIEKDGYTLYCTALFFSSEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGK 743
LVMGAIEK+GYTLYCT LFFS +G+FLGKHRK+MPTSLERCIWG GDGSTIPVYDTP+GK
Sbjct: 121 LVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGK 180
Query: 742 LGAAICWENRMPLFRTALYGKGIELYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSL 563
LGAAICWENRMPL+RTALY KGIELYCAPTADG+K WQSSM+HIAIEGGCFVLSACQF
Sbjct: 181 LGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQ 240
Query: 562 RKDFPDHADYLFTDPSSGMYDGEHQDTIVSQGGSVIISPLGKILAGPNFESEGLITADLD 383
RK FPDH DYLFTD YD + D+IVSQGGSVIISPLG++LAGPNFESEGL+TAD+D
Sbjct: 241 RKHFPDHPDYLFTD----WYDDKEHDSIVSQGGSVIISPLGQVLAGPNFESEGLVTADID 296
Query: 382 LGDIARAKLYFDVVGHYSRPEIFNLTVNENQKKPVTYVSKSVKAADDS 239
LGDIARAKLYFD VGHYSRP++ +LTVNE+ +K VT+V+K KA DDS
Sbjct: 297 LGDIARAKLYFDSVGHYSRPDVLHLTVNEHPRKSVTFVTKVEKAEDDS 344
>sp|P46010|NRL3_ARATH Nitrilase 3 OS=Arabidopsis thaliana GN=NIT3 PE=1 SV=1
Length = 346
Score = 532 bits (1370), Expect = 2e-150
Identities = 257/348 (73%), Positives = 301/348 (86%), Gaps = 5/348 (1%)
Frame = -1
Query: 1282 SSTEDMSKALKGTTPTFSDMPSTIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQL 1103
SSTE+MS ++K TT PS+ VR TIVQ+STVYNDTP T++KA +FI +AAS GA+L
Sbjct: 2 SSTEEMS-SVKNTTQVIGVDPSSTVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKL 60
Query: 1102 VLFPEGYIGGYPRGYRFGIAVGVHNEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVY 923
VLFPE +IGGYPRG+RFG+AVGVHNE+G D FR YHASAI VPGPEV++LAE+A K+ V+
Sbjct: 61 VLFPEAFIGGYPRGFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVH 120
Query: 922 LVMGAIEKDGYTLYCTALFFSSEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGK 743
LVMGAIEKDGYTLYCTALFFS +G+FLGKHRKVMPTSLERCIWG GDGSTIPVYDTP+GK
Sbjct: 121 LVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGK 180
Query: 742 LGAAICWENRMPLFRTALYGKGIELYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSL 563
+GAAICWENRMPL+RTALY KGIE+YCAPTAD + WQ+SM+HIA+EGGCFVLSA QF
Sbjct: 181 IGAAICWENRMPLYRTALYAKGIEIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCK 240
Query: 562 RKDFPDHADYLFTDPSSGMYDGEHQDTIVSQGGSVIISPLGKILAGPNFESEGLITADLD 383
R++FP+H DYLF D + D + D VS GGSVIISPLGK+LAGPN+ESEGL+TADLD
Sbjct: 241 RREFPEHPDYLFND----IVDTKEHDPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLD 296
Query: 382 LGDIARAKLYFDVVGHYSRPEIFNLTVNENQKKPVTYVSKSVKAADDS 239
LGDIARAKLYFDVVGHYS+P+IFNLTVNE+ KKPVT+++K KA D+S
Sbjct: 297 LGDIARAKLYFDVVGHYSKPDIFNLTVNEHPKKPVTFMTKVEKAEDES 344
>sp|P46011|NRL4_ARATH Bifunctional nitrilase/nitrile hydratase NIT4
OS=Arabidopsis thaliana GN=NIT4 PE=1 SV=1
Length = 355
Score = 442 bits (1136), Expect = 3e-123
Identities = 210/321 (65%), Positives = 257/321 (80%), Gaps = 4/321 (1%)
Frame = -1
Query: 1219 STIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAV 1040
S+IVRAT+VQASTV+ DTP T++KA +++AA +G+QLV+FPE +IGGYPRG F +A+
Sbjct: 33 SSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAI 92
Query: 1039 GVHNEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFS 860
G G D FRKYHASAI VPGPEV++LA MA+K+KVYLVMG IE++GYTLYCT LFF
Sbjct: 93 GSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKVYLVMGVIEREGYTLYCTVLFFD 152
Query: 859 SEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGK 680
S+G FLGKHRK+MPT+LERCIWG GDGSTIPV+DTP+GK+GAAICWENRMP RTA+Y K
Sbjct: 153 SQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIGKIGAAICWENRMPSLRTAMYAK 212
Query: 679 GIELYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFTDPSSGMYD 500
GIE+YCAPTAD + W +SM HIA+EGGCFVLSA QF RKD+P +Y+F SG +
Sbjct: 213 GIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFCRRKDYPSPPEYMF----SGSEE 268
Query: 499 GEHQDTIVSQGGSVIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDVVGHYSRPE 320
D++V GGS IISPLG +LAGPN+ E LITADLDLGDIARAK FDVVGHYSRPE
Sbjct: 269 SLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGDIARAKFDFDVVGHYSRPE 328
Query: 319 IFNLTVNENQKKPVTYVSKSV 257
+F+L + E+ +K V++ + V
Sbjct: 329 VFSLNIREHPRKAVSFKTSKV 349
>sp|Q42966|NRL4B_TOBAC Bifunctional nitrilase/nitrile hydratase NIT4B
OS=Nicotiana tabacum GN=NIT4B PE=2 SV=1
Length = 348
Score = 441 bits (1132), Expect = 9e-123
Identities = 215/320 (67%), Positives = 255/320 (79%), Gaps = 5/320 (1%)
Frame = -1
Query: 1210 VRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVGVH 1031
VRAT+VQAST++ DTP T++KA +A+AAS GAQLV+FPE +IGGYPRG FG+++G
Sbjct: 29 VRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNR 88
Query: 1030 NEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFSSEG 851
G + FRKYHASAI VPGPEVD+LA MA K+KVYLVMG IE+DGYTLYCT LFF S+G
Sbjct: 89 TAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGYTLYCTVLFFDSQG 148
Query: 850 RFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGKGIE 671
+LGKHRK+MPT+LER IWG GDGSTIPVYDTPLGK+GAAICWENRMPL RTA+Y KGIE
Sbjct: 149 HYLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRMPLLRTAMYAKGIE 208
Query: 670 LYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFTDPSSGMYDGEH 491
+YCAPTAD WQ+SM HIA+EGGCFVLSA QF RKD+P +Y+F SG D
Sbjct: 209 IYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVF----SGTED-LT 263
Query: 490 QDTIVSQGGSVIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDVVGHYSRPEIFN 311
D+IV GGSVIISP G +LAGPN+E E LI+ADLDLG+IARAK FDVVGHY+RPE+ +
Sbjct: 264 PDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAKFDFDVVGHYARPEVLS 323
Query: 310 LTVNENQKKPVTYVSKSVKA 251
L V ++ PV++ S S KA
Sbjct: 324 LIVRDHAVSPVSFTSTSSKA 343
>sp|Q42965|NRL4A_TOBAC Bifunctional nitrilase/nitrile hydratase NIT4A
OS=Nicotiana tabacum GN=NIT4A PE=2 SV=1
Length = 349
Score = 440 bits (1131), Expect = 1e-122
Identities = 214/320 (66%), Positives = 253/320 (79%), Gaps = 4/320 (1%)
Frame = -1
Query: 1210 VRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVGVH 1031
VRAT+VQAST++ DTP T+ KA +A+AAS GAQLV+FPE +IGGYPRG FG+++G
Sbjct: 29 VRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNR 88
Query: 1030 NEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFSSEG 851
G + FRKYHASAI VPGPEVD+LA MA K+KVYLVMG IE+DGYTLYCT LFF S+G
Sbjct: 89 TAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGYTLYCTVLFFDSQG 148
Query: 850 RFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGKGIE 671
FLGKHRK+MPT+LER IWG GDGSTIPVYDTPLGK+GAAICWENRMPL RTA+Y KGIE
Sbjct: 149 HFLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRMPLLRTAMYAKGIE 208
Query: 670 LYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFTDPSSGMYDGEH 491
+YCAPTAD WQ+SM HIA+EGGCFVLSA QF RKD+P +Y+F SG +
Sbjct: 209 IYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVF----SGTEEDLT 264
Query: 490 QDTIVSQGGSVIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDVVGHYSRPEIFN 311
D+IV GGSVIISP G +LAGPN+ E LI+ADLDLG+IARAK FDVVGHY+RPE+ +
Sbjct: 265 PDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLDLGEIARAKFDFDVVGHYARPEVLS 324
Query: 310 LTVNENQKKPVTYVSKSVKA 251
L V ++ PV++ S S KA
Sbjct: 325 LIVRDHAVSPVSFTSTSSKA 344
>sp|Q6H849|NRL4_ORYSJ Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza
sativa subsp. japonica GN=NIT4 PE=2 SV=1
Length = 362
Score = 429 bits (1102), Expect = 3e-119
Identities = 211/338 (62%), Positives = 259/338 (76%), Gaps = 11/338 (3%)
Frame = -1
Query: 1231 SDMPSTIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRF 1052
+D + VRAT+VQASTV+ DTP T++KA I +AA G+QLV+FPE ++GGYPRG F
Sbjct: 24 ADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAFVGGYPRGSTF 83
Query: 1051 G----IAVGVHNEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTL 884
G I++G + G + FRKYHA+AI VPGPEV +LA MA K+KV+LVMG IE++GYTL
Sbjct: 84 GFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRLAAMAGKYKVFLVMGVIEREGYTL 143
Query: 883 YCTALFFSSEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPL 704
YC+ LFF GR+LGKHRK+MPT+LER IWG GDGSTIPVYDTPLGK+GA ICWEN+MPL
Sbjct: 144 YCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGSTIPVYDTPLGKIGALICWENKMPL 203
Query: 703 FRTALYGKGIELYCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFT 524
RTALYGKGIE+YCAPTAD + WQ+SM HIA+EGGCFVLSA QF RKD+P +Y+F
Sbjct: 204 LRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVF- 262
Query: 523 DPSSGMYDGEHQDTIVSQGGSVIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDV 344
SG+ + DT+V GGSVIISP G++LAGPN+E E LITADLDLG+I RAK FDV
Sbjct: 263 ---SGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEALITADLDLGEIVRAKFDFDV 319
Query: 343 VGHYSRPEIFNLTVNENQKKPVTYVS---KSVKAADDS 239
VGHY+RPE+ +L VN+ PV++ S K+ A DS
Sbjct: 320 VGHYARPEVLSLVVNDQPHLPVSFTSAAEKTTAAKSDS 357
>sp|P40447|NIT1_YEAST Putative nitrilase-like protein NIT1 OS=Saccharomyces
cerevisiae GN=NIT1 PE=5 SV=1
Length = 199
Score = 179 bits (453), Expect = 5e-044
Identities = 83/187 (44%), Positives = 120/187 (64%), Gaps = 4/187 (2%)
Frame = -1
Query: 1195 VQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVGVHNEDGG 1016
+Q + T T++K + + GA+LV+ PE +GGYP+G FG+ +G ++G
Sbjct: 9 LQIGSCPGSTKDTLKKILSYEKEIKESGAKLVVIPEATLGGYPKGSNFGVYLGYRLQEGR 68
Query: 1015 DSFRKYHASAIAVPG----PEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFSSEGR 848
+ + KY A AI + PE+ +L +++ L +G IE+DG TLYCT ++ +
Sbjct: 69 EEYAKYLAEAIEIGNGEKYPEISQLCALSKATDASLCVGCIERDGTTLYCTMVYIDPKDG 128
Query: 847 FLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGKGIEL 668
++GKHRK+MPT+ ER IWG GDGST+PV DT GK+G AICWEN MPL R A+Y KG+E+
Sbjct: 129 YVGKHRKLMPTAGERLIWGQGDGSTLPVVDTAAGKIGGAICWENMMPLLRYAMYKKGVEI 188
Query: 667 YCAPTAD 647
+CAPT D
Sbjct: 189 WCAPTVD 195
>sp|P82605|NRL_BACSX Nitrilase OS=Bacillus sp. (strain OxB-1) GN=nit PE=1 SV=2
Length = 339
Score = 162 bits (409), Expect = 6e-039
Identities = 107/330 (32%), Positives = 166/330 (50%), Gaps = 30/330 (9%)
Frame = -1
Query: 1207 RATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVGVHN 1028
R VQAS V D TI+K + +AA++GA+++ FPE +I GYP G A
Sbjct: 8 RVAAVQASPVLLDLDATIDKTCRLVDEAAANGAKVIAFPEAFIPGYPWWIWLGNA----- 62
Query: 1027 EDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFSSEGR 848
+ G + + + +++ +P V KL+ A +KVY + EKDG +LY T L+F G
Sbjct: 63 DYGMKYYIQLYKNSVEIPSLAVQKLSS-AGTNKVYFCVSVTEKDGGSLYLTQLWFDPNGD 121
Query: 847 FLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGKGIEL 668
+GKHRK+ T+ E+ IWG GDGS +PV++T G LG CWE+ +PL A+ ++
Sbjct: 122 LIGKHRKLKATNAEKTIWGDGDGSMMPVFETEFGNLGGLQCWEHFLPLNVAAMASMNEQV 181
Query: 667 YCAPTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFTDPSSGMYDGEHQ 488
+ A +W M EG F C + + + ++ +F SS ++ E +
Sbjct: 182 HVA-------SWPIGMPQ---EGHLFGPEQCVTATK--YYAISNQVFCLLSSQIWTEEQR 229
Query: 487 DTI-----------VSQGGSVIISPLGKILAGP-NFESEGLITADLDLGDIARAKLYFDV 344
D I V G S II+P G + + EG+ AD+DL I K D
Sbjct: 230 DKICETEEQRNFMKVGHGFSKIIAPNGMEIGNKLAHDEEGITYADIDLEQIIPGKFLIDS 289
Query: 343 VGHYSRPEIFNLTVNENQKKPVTYVSKSVK 254
GHYS P +L+ + +KKP+ ++ +S +
Sbjct: 290 AGHYSTPGFLSLSFDRTEKKPIKHIGESAQ 319
>sp|Q02068|NRL1_RHORH Aliphatic nitrilase OS=Rhodococcus rhodochrous PE=1 SV=1
Length = 383
Score = 151 bits (379), Expect = 2e-035
Identities = 105/327 (32%), Positives = 160/327 (48%), Gaps = 25/327 (7%)
Frame = -1
Query: 1210 VRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVGVH 1031
V+ VQA V D TI+KA FI +AA +GA+ + FPE +I GYP + +G
Sbjct: 13 VKVATVQAEPVILDADATIDKAIGFIEEAAKNGAEFLAFPEVWIPGYP----YWAWIGDV 68
Query: 1030 NEDGGDSFRKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFSSEG 851
D KYH +++ + + +L AR++ + LVMG EKDG + Y + +F G
Sbjct: 69 KWAVSDFIPKYHENSLTLGDDRMRRLQLAARQNNIALVMGYSEKDGASRYLSQVFIDQNG 128
Query: 850 RFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGKGIE 671
+ RK+ PT +ER I+G G+G+ +D G++G CWE+ PL + +Y +
Sbjct: 129 DIVANRRKLKPTHVERTIYGEGNGTDFLTHDFGFGRVGGLNCWEHFQPLSKYMMYSLNEQ 188
Query: 670 LYCAP-------TAD----GTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFT 524
++ A T D + + AIEG FVL++ +
Sbjct: 189 IHVASWPAMFALTPDVHQLSVEANDTVTRSYAIEGQTFVLASTHVIGKAT---------Q 239
Query: 523 DPSSGMYDGEHQDTIVSQGGSVIISPLGKILAGPNFE-SEGLITADLDLGDIARAKLYFD 347
D +G D + + QG + I P GK LA P E +EGL+ A+LDL I AK D
Sbjct: 240 DLFAGDDDAKRALLPLGQGWARIYGPDGKSLAEPLPEDAEGLLYAELDLEQIILAKAAAD 299
Query: 346 VVGHYSRPEIFNLTVNENQKKPVTYVS 266
GHYSRP++ +L ++ PV Y++
Sbjct: 300 PAGHYSRPDVLSLKIDTRNHTPVQYIT 326
>sp|P10045|NRLB_KLEPO Nitrilase, bromoxynil-specific OS=Klebsiella pneumoniae
subsp. ozaenae GN=bxn PE=1 SV=1
Length = 349
Score = 139 bits (349), Expect = 5e-032
Identities = 105/339 (30%), Positives = 171/339 (50%), Gaps = 30/339 (8%)
Frame = -1
Query: 1216 TIVRATIVQASTVYNDTPKTIEKAGEFIAQAASDGAQLVLFPEGYIGGYPRGYRFGIAVG 1037
T +A VQA V+ D T +K +A+AA+ GAQLV FPE +I GYP G+
Sbjct: 3 TTFKAAAVQAEPVWMDAAATADKTVTLVAKAAAAGAQLVAFPELWIPGYP-GFML----- 56
Query: 1036 VHNEDGGDSF-RKYHASAIAVPGPEVDKLAEMARKHKVYLVMGAIEKDGYTLYCTALFFS 860
HN+ F KY AIA GPE++K+ A++H + L G E+ G TLY + +
Sbjct: 57 THNQTETLPFIIKYRKQAIAADGPEIEKIRCAAQEHNIALSFGYSERAGRTLYMSQMLID 116
Query: 859 SEGRFLGKHRKVMPTSLERCIWGCGDGSTIPVYDTPLGKLGAAICWENRMPLFRTALYGK 680
++G + RK+ PT ER ++G GDGS + V T +G++GA C EN L + AL +
Sbjct: 117 ADGITKIRRRKLKPTRFERELFGEGDGSDLQVAQTSVGRVGALNCAENLQSLNKFALAAE 176
Query: 679 GIELYC--------APTADGTKNWQSSMMHIAIEGGCFVLSACQFSLRKDFPDHADYLFT 524
G +++ +P G + ++ A E G FVL + Q
Sbjct: 177 GEQIHISAWPFTLGSPVLVGDSIGAINQVY-AAETGTFVLMSTQVVGPTGI--------- 226
Query: 523 DPSSGMYDGEHQDTIVSQGGSVIISPLGKILA-GPNFESEGLITADLDLGDIARAKLYFD 347
+ + D + + + G + I P ++ + + EG++ A++DL + AK D
Sbjct: 227 -AAFEIEDRYNPNQYLGGGYARIYGPDMQLKSKSLSPTEEGIVYAEIDLSMLEAAKYSLD 285
Query: 346 VVGHYSRPEIFNLTVNENQKKPVTYVSKSVKAADDSEPQ 230
GHYSRP++F++++N Q++P VS+ + + D +P+
Sbjct: 286 PTGHYSRPDVFSVSIN-RQRQPA--VSEVIDSNGDEDPR 321
>sp|P40446|YIQ5_YEAST Putative nitrilase-like protein YIL165C OS=Saccharomyces
cerevisiae GN=YIL165C PE=5 SV=1
Length = 119
Score = 74 bits (180), Expect = 2e-012
Identities = 48/115 (41%), Positives = 64/115 (55%), Gaps = 11/115 (9%)
Frame = -1
Query: 622 MMHIAIEGGCFVLSACQFSLRKDFPDHADYLF---TDPSSG---MYDGEHQDTIVSQGGS 461
M +IA EG F++SA QF PD F D ++G + D GGS
Sbjct: 1 MKNIAYEGRLFLISAVQF-----MPDATAMGFGEIIDQATGKRKLPGWPSADDNCINGGS 55
Query: 460 VIISPLGKILAGPNFESEGLITADLDLGDIARAKLYFDVVGHYSRPEIFNLTVNE 296
VII P G+I+AGP EGL+TA+++ IA A+ D VGHY+R ++F LTVNE
Sbjct: 56 VIIDPYGEIIAGPLLGQEGLLTAEINTDLIAEARFDLDPVGHYARGDVFQLTVNE 110
>sp|P20960|NRLA_ALCFA Nitrilase, arylacetone-specific OS=Alcaligenes faecalis
PE=1 SV=2
Length = 356
Score = 62 bits (150), Expect = 6e-009
Identities = 41/94 (43%), Positives = 60/94 (63%), Gaps = 6/94 (6%)
Frame = -1
Query: 499 GEHQDTIVSQGG--SVIISPLGKILAG--PNFESEGLITADLDLGDIARAKLYFDVVGHY 332
GEH +++ GG S+I +P G+ LA P+ ++EGLI ADL++ +IA AK D VGHY
Sbjct: 238 GEHNASLLKVGGGSSMIFAPDGRTLAPYLPH-DAEGLIIADLNMEEIAFAKAINDPVGHY 296
Query: 331 SRPEIFNLTVNENQKKPVTYV-SKSVKAADDSEP 233
S+PE L ++ ++P+T V SKSV + EP
Sbjct: 297 SKPEATRLVLDLGHREPMTRVHSKSVIQEEAPEP 330
>sp|Q03217|NRL2_RHORH Aliphatic nitrilase OS=Rhodococcus rhodochrous GN=nitA
PE=1 SV=2
Length = 366
Score = 62 bits (149), Expect = 8e-009
Identities = 36/94 (38%), Positives = 50/94 (53%), Gaps = 3/94 (3%)
Frame = -1
Query: 502 DGEHQDTIVSQGGSV--IISPLGKILAGPNFE-SEGLITADLDLGDIARAKLYFDVVGHY 332
D + Q ++ +GG II P G+ LA P E EG++ AD+DL I AK D VGHY
Sbjct: 239 DNDEQRKLIGRGGGFARIIGPDGRDLATPLAEDEEGILYADIDLSAITLAKQAADPVGHY 298
Query: 331 SRPEIFNLTVNENQKKPVTYVSKSVKAADDSEPQ 230
SRP++ +L N+ PV ++ A PQ
Sbjct: 299 SRPDVLSLNFNQRHTTPVNTAISTIHATHTLVPQ 332
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,472,851,095
Number of Sequences: 518415
Number of Extensions: 69472851095
Number of Successful Extensions: 496854919
Number of sequences better than 0.0: 0
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