BLASTX 7.6.2
Query= UN19932 /QuerySize=1184
(1183 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 190 2e-047
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 187 1e-046
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 170 2e-041
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 166 4e-040
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 163 2e-039
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 151 9e-036
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 150 2e-035
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 149 3e-035
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 148 8e-035
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 148 1e-034
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 148 1e-034
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 147 2e-034
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 147 2e-034
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 119 5e-026
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 116 4e-025
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 110 2e-023
sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus... 102 6e-021
sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegi... 97 2e-019
sp|O75845|SC5D_HUMAN Lathosterol oxidase OS=Homo sapiens GN=SC5D... 67 3e-010
sp|O13666|ERG32_SCHPO Probable C-5 sterol desaturase 2 OS=Schizo... 57 2e-007
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 190 bits (482), Expect = 2e-047
Identities = 90/260 (34%), Positives = 147/260 (56%), Gaps = 8/260 (3%)
Frame = -1
Query: 1060 NLTWFETVWFDY-SATKSDFHVYCHTILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMK 884
+L + E WF + DF + T + + + + +PF + + +++KIQP K
Sbjct: 5 SLKFIEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKK 64
Query: 883 YSLSDMFRCYKDVMKLFLLVVGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIE 704
F C V+ + V + ++ P+I+ +G+ + PLPS+ + + FLIE
Sbjct: 65 NEWKTQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIE 124
Query: 703 DYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGH 524
D+ YW+HR LH + Y+ IH+VHH++ +P G + YAH E ++LG+ T +GP +
Sbjct: 125 DFYFYWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRD 184
Query: 523 IMTFWLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASV 350
+ T W+W+ R + +E HSGYDFPW TKL+PF+GG+ +HD+HH +VG N++S
Sbjct: 185 LFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHETFVG-----NYSST 239
Query: 349 FTYCDYIYGTDKGYRVHKKL 290
FTY D I+GT Y K++
Sbjct: 240 FTYLDKIFGTSDKYYSRKQI 259
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 187 bits (474), Expect = 1e-046
Identities = 94/264 (35%), Positives = 147/264 (55%), Gaps = 7/264 (2%)
Frame = -1
Query: 1060 NLTWFETVWFDYSATKSDFHVYCHTILVLFLVFSLAP-LPFVFVELTGWFERFKIQPKMK 884
+L + E WF + + + + + VF +PF+ + +F ++KIQ +
Sbjct: 5 SLKFIEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKE 64
Query: 883 YSLSDMFRCYKDVMKLFLLVVGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIE 704
C V+ +L+ + ++ P+I+ +G+ + PLPS+ + LV F+IE
Sbjct: 65 NDWKSQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIE 124
Query: 703 DYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGH 524
D+ YW HR LH Y+ IH+VHH+Y SP G + YAH E ++LG+ T +GP +
Sbjct: 125 DFYFYWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRD 184
Query: 523 IMTFWLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHHYVGGQSQSNFASVFT 344
+ T W+W+ +R + +E HSGYDFPW+ T L+PF+GGA +HDYHH V N+AS FT
Sbjct: 185 LFTLWVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEV---FIGNYASTFT 241
Query: 343 YCDYIYGTD-KGY--RVHKKLLQQ 281
Y D I+GT K Y R+ KK + +
Sbjct: 242 YLDKIFGTSGKSYYSRIEKKSINK 265
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 170 bits (430), Expect = 2e-041
Identities = 86/265 (32%), Positives = 144/265 (54%), Gaps = 12/265 (4%)
Frame = -1
Query: 1036 WFDYSATKSDFHVYCHTILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRC 857
WFD + + L+ L++ +P++ ++ +F R+KIQPK +L++ + C
Sbjct: 40 WFDNDVVATGL----MSFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKKVPTLAEQWEC 95
Query: 856 YKDVMKLFLLVVGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHR 677
+ V+ V ++ P G+ + +P P + + + Q+ ++F +ED +YW HR
Sbjct: 96 TRLVLLSHFTVELPQIWLFDPMCATFGLSTSVPFPPVTKMIWQITLFFFLEDTWHYWAHR 155
Query: 676 WLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAI-----APGHIMTF 512
H Y IH+VHH Y++P G ++ YAH E++LLG T P + H++T
Sbjct: 156 LFHYGIFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVPLMWCYFTHDLHLVTM 215
Query: 511 WLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDY 332
++WITLR +A+++H+GYDFPW+L K +P + GA++HDYHH + NF+S F + D
Sbjct: 216 YIWITLRLFQAVDSHAGYDFPWSLNKFLPIWAGADHHDYHHMA---FKDNFSSSFRWWDA 272
Query: 331 IYGTDKGYRVHKKLLQQITEEAEQK 257
+ TD+ Y K EAE K
Sbjct: 273 VLKTDQNYHQFKARRLAAKYEAESK 297
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 166 bits (419), Expect = 4e-040
Identities = 86/241 (35%), Positives = 131/241 (54%), Gaps = 10/241 (4%)
Frame = -1
Query: 949 LPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLFLLVVGTLQFVTYPSIQLVGIR 770
LP+ ++ +F R+K+QP S + C K V+ LV + +P + +GI
Sbjct: 71 LPWFIIDQIPYFRRWKLQPTKIPSAKEQLYCLKSVLLSHFLVEAIPIWTFHPMCEKLGIT 130
Query: 769 SGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYA 590
+P PSL ++ ++F++ED +YW HR H Y+ IH+ HH Y +P G ++ YA
Sbjct: 131 VEVPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYA 190
Query: 589 HWAEVLLLGIPTFLGPAI-----APGHIMTFWLWITLRQMEAIETHSGYDFPWALTKLVP 425
H AE L LG T P + H+ T +WITLR +A+++HSGYDFPW+L K++P
Sbjct: 191 HPAETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMP 250
Query: 424 FYGGAEYHD-YHHYVGGQSQSNFASVFTYCDYIYGTDKGYRVHKKLLQQITEEAEQKGGT 248
F+ GAE+HD +HHY G N+AS F + DY T+ G +++ + AE
Sbjct: 251 FWAGAEHHDLHHHYFIG----NYASSFRWWDYCLDTESGPEAKASREERMKKRAENNAQK 306
Query: 247 K 245
K
Sbjct: 307 K 307
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 163 bits (412), Expect = 2e-039
Identities = 91/282 (32%), Positives = 145/282 (51%), Gaps = 26/282 (9%)
Frame = -1
Query: 1060 NLTWFETVWFDYSATKSDFHVYCHTILVLFLVFSLAP--------LPFVFVELTGWFERF 905
NL FE +W Y ++ + + L+F L LP+ ++ +F ++
Sbjct: 32 NLNVFEKLWGSY------YYYMANDLFATGLLFFLTHEIFYFGRCLPWAIIDRIPYFRKW 85
Query: 904 KIQPKMKYSLSDMFRCYKDVMKLFLLVVGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQL 725
KIQ + S + + C K V+ LV + +P Q +GI +P P + + + Q
Sbjct: 86 KIQDEKIPSDKEQWECLKSVLTSHFLVEAFPIWFFHPLCQKIGISYQVPFPKITDMLIQW 145
Query: 724 VVYFLIEDYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLG 545
V+F++ED +YW HR LH Y+ IH+ HH Y +P G A+ YAH EV LLG+ T
Sbjct: 146 AVFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGTVGI 205
Query: 544 PAI-----APGHIMTFWLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHH--Y 386
P + H+ T +WI LR +A++ HSGY+FPW+L +PF+ GA++HD HH +
Sbjct: 206 PIVWCLITGNLHLFTVSIWIILRLFQAVDAHSGYEFPWSLHNFLPFWAGADHHDEHHHYF 265
Query: 385 VGGQSQSNFASVFTYCDYIYGTDKGYRVHKKLLQQITEEAEQ 260
+GG S S F + D+I T+ G + K ++ + E+
Sbjct: 266 IGGYSSS-----FRWWDFILDTEAGPKAKKGREDKVKQNVEK 302
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 151 bits (381), Expect = 9e-036
Identities = 84/239 (35%), Positives = 129/239 (53%), Gaps = 16/239 (6%)
Frame = -1
Query: 988 TILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVM--KLFL---LV 824
++LV + L +P + + +++KIQP + ++C+K ++ F+ L+
Sbjct: 54 SLLVHEASYFLLCVPGFIFQFIPYMQKYKIQPDKPETWEKQWKCFKTLIFNHFFIQLPLI 113
Query: 823 VGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMI 644
GT F Y +I +P +AQ +IED +Y++HR LH K Y+ I
Sbjct: 114 CGTYYFTEYFNIPY----EWEEMPRWYVLLAQCFGCAVIEDAWHYFLHRLLHHKRIYKYI 169
Query: 643 HRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHS 464
H+VHHE+ SP G + YAH E L+LG F+G + H++ W W+ R ME I+ HS
Sbjct: 170 HKVHHEFVSPFGMQAEYAHPLETLILGAGFFIGIVVFCNHVVLLWAWVICRLMETIDVHS 229
Query: 463 GYDFPWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVHKK 293
GYD P LVPFY GA +HD+HH ++G N+AS FT+ D I+GTD + +K+
Sbjct: 230 GYDIPLNPLHLVPFYAGARFHDFHHMNFIG-----NYASTFTWWDRIFGTDSQFIAYKE 283
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 150 bits (378), Expect = 2e-035
Identities = 81/237 (34%), Positives = 130/237 (54%), Gaps = 16/237 (6%)
Frame = -1
Query: 988 TILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVM--KLFL---LV 824
+++V ++ L LP + + ++KIQ + ++C K ++ F+ L+
Sbjct: 54 SLIVHEAIYFLFSLPGFLFQFIPYMRKYKIQKDKPETFEGQWKCLKKILFNHFFIQLPLI 113
Query: 823 VGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMI 644
GT F + +I R +P T+A+ + +IED +Y++HR LH K Y+ I
Sbjct: 114 CGTYYFTEFFNIPYDWER----MPRWYLTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYI 169
Query: 643 HRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHS 464
H+VHHE+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HS
Sbjct: 170 HKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHS 229
Query: 463 GYDFPWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVH 299
GYD P LVPFY GA +HD+HH ++G N+AS FT+ D ++GTD Y +
Sbjct: 230 GYDIPLNPLNLVPFYTGARHHDFHHMNFIG-----NYASTFTWWDKLFGTDAQYHAY 281
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 149 bits (376), Expect = 3e-035
Identities = 80/244 (32%), Positives = 134/244 (54%), Gaps = 16/244 (6%)
Frame = -1
Query: 988 TILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVM--KLFL---LV 824
+++V ++ L LP + + ++KIQ + ++C K ++ F+ L+
Sbjct: 54 SLIVHETIYFLFSLPGFLFQFIPFMRKYKIQKDKPETFEGQWKCLKGILFNHFFIQLPLI 113
Query: 823 VGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMI 644
GT F + +I R +P T+A+ + +IED +Y++HR LH K Y+ I
Sbjct: 114 CGTYYFTEFFNIPYDWER----MPRWYFTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYI 169
Query: 643 HRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHS 464
H+VHHE+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HS
Sbjct: 170 HKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTMRLLETIDVHS 229
Query: 463 GYDFPWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVHKKL 290
GYD P +PFY GA +HD+HH ++G N+AS FT+ D I+GTD Y + +
Sbjct: 230 GYDIPLNPLNYIPFYTGARHHDFHHMNFIG-----NYASTFTWWDRIFGTDVQYHAYTEK 284
Query: 289 LQQI 278
++++
Sbjct: 285 MKKL 288
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 148 bits (373), Expect = 8e-035
Identities = 83/239 (34%), Positives = 128/239 (53%), Gaps = 20/239 (8%)
Frame = -1
Query: 988 TILVLFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLF-------L 830
+++V L++ L LP + + +++KIQP + ++C+K M LF
Sbjct: 54 SLIVHELIYFLFCLPGFIFQFLPFMQKYKIQPDKPETWEKQWKCFK--MLLFNHFCIQLP 111
Query: 829 LVVGTLQFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYE 650
L+ GT F + SI +P +AQ +IED +Y++HR LH + Y+
Sbjct: 112 LICGTYYFTEFFSIPY----DWDTMPRWPFLLAQCFGCAVIEDTWHYFLHRALHHRRIYK 167
Query: 649 MIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIET 470
IH+VHH++TSP G + YAH E L+LG F+G + H++ W W+T R +E I+
Sbjct: 168 YIHKVHHDFTSPFGMQAEYAHPLETLILGAGFFIGTMVFCNHMILLWAWVTFRLLETIDV 227
Query: 469 HSGYDFPWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVH 299
HSGYD P L+PFY GA +HD+HH +VG N+ S FT+ D ++ TD + H
Sbjct: 228 HSGYDIPLNPLHLIPFYAGARFHDFHHMNFVG-----NYGSTFTWWDRLFDTDSQFNKH 281
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 148 bits (371), Expect = 1e-034
Identities = 80/235 (34%), Positives = 128/235 (54%), Gaps = 19/235 (8%)
Frame = -1
Query: 976 LFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLFL-----LVVGTL 812
L+ +F L F F+ + +++KIQ + + ++C+K ++ L+ GT
Sbjct: 61 LYFLFCLPGFLFQFIP---YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTY 117
Query: 811 QFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVH 632
F Y +I R +P +A+ +IED +Y++HR LH K Y+ IH+VH
Sbjct: 118 YFTEYFNIPYDWER----MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVH 173
Query: 631 HEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDF 452
HE+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HSGYD
Sbjct: 174 HEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDI 233
Query: 451 PWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVHKK 293
P L+PFY G+ +HD+HH ++G N+AS FT+ D I+GTD Y + +
Sbjct: 234 PLNPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYNAYNE 283
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 148 bits (371), Expect = 1e-034
Identities = 80/235 (34%), Positives = 128/235 (54%), Gaps = 19/235 (8%)
Frame = -1
Query: 976 LFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLFL-----LVVGTL 812
L+ +F L F F+ + +++KIQ + + ++C+K ++ L+ GT
Sbjct: 61 LYFLFCLPGFLFQFIP---YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTY 117
Query: 811 QFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVH 632
F Y +I R +P +A+ +IED +Y++HR LH K Y+ IH+VH
Sbjct: 118 YFTEYFNIPYDWER----MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVH 173
Query: 631 HEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDF 452
HE+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HSGYD
Sbjct: 174 HEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDI 233
Query: 451 PWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVHKK 293
P L+PFY G+ +HD+HH ++G N+AS FT+ D I+GTD Y + +
Sbjct: 234 PLNPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYNAYNE 283
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 147 bits (370), Expect = 2e-034
Identities = 84/243 (34%), Positives = 131/243 (53%), Gaps = 20/243 (8%)
Frame = -1
Query: 970 LVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLFL-----LVVGTLQF 806
L FS LP + + +++KIQ + + ++C+K ++ L+ GT F
Sbjct: 61 LYFSFC-LPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYF 119
Query: 805 VTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVHHE 626
Y +I R +P +A+ +IED +Y++HR LH K Y+ IH+VHHE
Sbjct: 120 TEYFNIPYDWER----MPRWYFLLARCFGCAVIEDTWHYFMHRLLHHKRIYKYIHKVHHE 175
Query: 625 YTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPW 446
+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HSGYD P
Sbjct: 176 FQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPL 235
Query: 445 ALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVH---KKLLQQ 281
L+PFY G+ +HD+HH ++G N+AS FT+ D I+GTD Y + KK ++
Sbjct: 236 NPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYHAYYEKKKKFEK 290
Query: 280 ITE 272
TE
Sbjct: 291 KTE 293
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 147 bits (369), Expect = 2e-034
Identities = 80/235 (34%), Positives = 127/235 (54%), Gaps = 19/235 (8%)
Frame = -1
Query: 976 LFLVFSLAPLPFVFVELTGWFERFKIQPKMKYSLSDMFRCYKDVM-----KLFLLVVGTL 812
L+ F L F F+ + +++KIQ + + ++C+K ++ F L+ GT
Sbjct: 61 LYFFFCLPGFLFQFIP---YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQFPLICGTY 117
Query: 811 QFVTYPSIQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVH 632
F Y SI +P +A+ +IED +Y++HR LH K Y+ IH++H
Sbjct: 118 YFTEYFSIPY----DWETMPRWYIALARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKIH 173
Query: 631 HEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDF 452
HE+ +P G + YAH E L+LG F+G + H++ W W+T+R +E I+ HSGYD
Sbjct: 174 HEFQAPFGMEAEYAHPLETLILGTGFFIGIMLLCDHVILLWAWVTVRLIETIDVHSGYDI 233
Query: 451 PWALTKLVPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRVHKK 293
P L+PFY G+ +HD+HH ++G N+AS FT+ D I+GTD Y + +
Sbjct: 234 PLNPLHLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDAQYNAYNE 283
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 119 bits (297), Expect = 5e-026
Identities = 73/207 (35%), Positives = 111/207 (53%), Gaps = 14/207 (6%)
Frame = -1
Query: 850 DVMKLFLLVVGTLQFV-TYPS--IQLVGI-RSGLPL--PSLMETVAQLVVYFLIEDYTNY 689
D+MK L + G Q + YP +QL+ + + LP+ P++ E V+QL ++FL D+T +
Sbjct: 87 DLMKKSLKLQGWNQLLWIYPMALVQLIWVPDTELPILAPTVFEMVSQLAIFFLAFDFTYF 146
Query: 688 WIHRWLH-CKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTF 512
W H + H KW Y H VHH Y+SP ++ + H E+ + P I P H +T+
Sbjct: 147 WFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVATFITTVPWIFPTHCLTY 206
Query: 511 WLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDY 332
WLW + Q + E H GYDFP+AL ++ FY GA HD HH + + F F Y D
Sbjct: 207 WLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHL---RPLTCFQPWFNYLDR 263
Query: 331 IYGTDKGYRVHKKLLQQITEEAEQKGG 251
+ GY + + L+++TE +K G
Sbjct: 264 L----MGYHITYEDLKKMTEAKFKKFG 286
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 116 bits (289), Expect = 4e-025
Identities = 78/237 (32%), Positives = 121/237 (51%), Gaps = 22/237 (9%)
Frame = -1
Query: 940 VFVELTGWFERFKIQPKMKYSLSDMFRCYKDVMKLFLLVVGTLQFV-TYPS--IQLVGI- 773
VF+ +F+ KIQ K + D++K L + G Q + YP +QL+ +
Sbjct: 65 VFLCNVPFFKDAKIQKDRKVTW--------DLIKKSLKLQGWNQLLWIYPMALVQLIWVP 116
Query: 772 RSGLPL--PSLMETVAQLVVYFLIEDYTNYWIHRWLH-CKWGYEMIHRVHHEYTSPIGYA 602
+ LP+ P++ E ++QL ++FL D+T +W H H KW Y H VHH Y+SP +
Sbjct: 117 DTELPILAPTVFEMLSQLAIFFLAFDFTYFWFHYINHKVKWLYRWCHSVHHMYSSPFAAS 176
Query: 601 SPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPWALTKLVPF 422
+ + H E+ +G P I P H +T+W+W + Q + E H GYDFP+AL ++ F
Sbjct: 177 AQHLHPFELFFVGTFITTIPWIFPTHCLTYWIWFFIAQSVSYEVHIGYDFPFALHRIFWF 236
Query: 421 YGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRVHKKLLQQITEEAEQKGG 251
Y GA HD HH + + F F Y D + GY + L+++TE +K G
Sbjct: 237 YSGAPAHDMHHL---RPLTCFQPWFNYLDRL----MGYHITYADLKKMTEAKFKKFG 286
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 110 bits (274), Expect = 2e-023
Identities = 70/229 (30%), Positives = 116/229 (50%), Gaps = 20/229 (8%)
Frame = -1
Query: 949 LPFVFVELTG--W--FERFKIQPKMKYSLSDMFRCYKDVMKLFLLVV---GTLQFVTYPS 791
LP++ ++ G W R+KIQP + + + C + +L+V Q++ P
Sbjct: 54 LPYLSCDILGRKWPAIYRYKIQPDKLPTTAMLLHCSGVTLYNHILLVIPAAVAQWMWRPP 113
Query: 790 IQLVGIRSGLPLPSLMETVAQLVVYFLIEDYTNYWIHRWLHCK--WGYEMIHRVHHEYTS 617
I L P+L+E V + L+ D Y+I +LH K W Y H +HH Y++
Sbjct: 114 IPLPE-----QAPTLLELVGGVTGNLLLFD-LQYFIWHFLHHKIRWLYVTFHAIHHNYSA 167
Query: 616 PIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPWALT 437
P A+ E++ +G T L P + H++T W+++ + ++E H GYDFPW+ +
Sbjct: 168 PFALATQCLGGWELVTVGFWTTLNPVLLRCHLLTTWMFMVVHVYVSVEDHCGYDFPWSTS 227
Query: 436 KLVPF--YGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRVHK 296
+L+PF YGG HD HH + +NFA F++ D ++GT +R K
Sbjct: 228 RLIPFGVYGGPSKHDVHHQ---KPNTNFAPHFSHWDKMFGTHADFRFSK 273
>sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus GN=Ch25h PE=1
SV=1
Length = 298
Score = 102 bits (253), Expect = 6e-021
Identities = 75/222 (33%), Positives = 110/222 (49%), Gaps = 23/222 (10%)
Frame = -1
Query: 949 LPFVFVE-LTGW---FERFKIQPKMKYSLSDMFRCYKDVMKLFLLVVGTLQFVTYPSIQL 782
LPFV ++ L W R+KI P S+ + C L L + L FV +P L
Sbjct: 53 LPFVVLDVLYPWVPILRRYKIHPDFSPSVKQLLPC------LGLTLYQHLVFV-FPVTLL 105
Query: 781 VGIRSGLPL----PSLMETVAQLVVYFLIEDYTNYWIHRWLHCK--WGYEMIHRVHHEYT 620
+RS L P L++ ++ +++ L+ D T + LH K W Y H+VHH+ +
Sbjct: 106 HWVRSPALLPQEAPELVQLLSHVLICLLLFD-TEIFAWHLLHHKVPWLYRTFHKVHHQNS 164
Query: 619 SPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPWAL 440
S A+ Y + E+L L L A+ H +T + + + ++E HSGYDFPW+
Sbjct: 165 SSFALATQYMSFWELLSLTFFDVLNVAVLRCHPLTIFTFHVINIWLSVEDHSGYDFPWST 224
Query: 439 TKLVPF--YGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGT 320
+LVPF YGG +HD HH Q NFA FT+ D + GT
Sbjct: 225 HRLVPFGWYGGVAHHDMHH---SQFNCNFAPYFTHWDKMLGT 263
>sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegicus GN=Ch25h
PE=2 SV=1
Length = 298
Score = 97 bits (241), Expect = 2e-019
Identities = 77/234 (32%), Positives = 113/234 (48%), Gaps = 25/234 (10%)
Frame = -1
Query: 979 VLFLVFSLAP--LPFVFVE-LTGW---FERFKIQPKMKYSLSDMFRCYKDVMKLFLLVVG 818
V+F +F+ LPFV ++ L W R+KI P S+ + C L L +
Sbjct: 41 VIFSIFTYLGFCLPFVVLDVLCPWVPILRRYKIHPDFSPSVRQLLPC------LGLTLYQ 94
Query: 817 TLQFVTYPSIQLVGIRSGLPL----PSLMETVAQLVVYFLIEDYTNYWIHRWLHCK--WG 656
L FV +P + RS L P L + ++ +++ L+ D T + LH K W
Sbjct: 95 HLVFV-FPVTLMHWARSPALLPREAPELSQLLSHVLICLLLFD-TEIFAWHLLHHKVPWL 152
Query: 655 YEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFLGPAIAPGHIMTFWLWITLRQMEAI 476
Y H+VHH+ +S A+ Y E+L L L A+ H +T ++ + ++
Sbjct: 153 YRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWLSV 212
Query: 475 ETHSGYDFPWALTKLVPF--YGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGT 320
E HSGYDFPW+ +LVPF YGG +HD HH Q NFA FT+ D + GT
Sbjct: 213 EDHSGYDFPWSTHRLVPFGWYGGVAHHDLHH---SQFNCNFAPYFTHWDKMLGT 263
>sp|O75845|SC5D_HUMAN Lathosterol oxidase OS=Homo sapiens GN=SC5DL PE=1 SV=2
Length = 299
Score = 67 bits (161), Expect = 3e-010
Identities = 45/145 (31%), Positives = 64/145 (44%), Gaps = 5/145 (3%)
Frame = -1
Query: 748 LMETVAQLVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLL 569
L E V ++ + D YWIHR LH + Y+ +H+ HH + P +AS H + L
Sbjct: 116 LFELVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFL 175
Query: 568 LGIPTFLGPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHH 389
+P + P I P H + + L + I H G DF L PF G+ +H HH
Sbjct: 176 QSLPYHIYPFIFPLHKVVYLSLYILVNIWTISIHDG-DFR-VPQILQPFINGSAHHTDHH 233
Query: 388 YVGGQSQSNFASVFTYCDYIYGTDK 314
N+ FT D I G+ K
Sbjct: 234 MF---FDYNYGQYFTLWDRIGGSFK 255
>sp|O13666|ERG32_SCHPO Probable C-5 sterol desaturase 2 OS=Schizosaccharomyces
pombe GN=SPBC27B12.03c PE=2 SV=1
Length = 329
Score = 57 bits (137), Expect = 2e-007
Identities = 32/127 (25%), Positives = 54/127 (42%), Gaps = 5/127 (3%)
Frame = -1
Query: 727 LVVYFLIEDYTNYWIHRWLHCKWGYEMIHRVHHEYTSPIGYASPYAHWAEVLLLGIPTFL 548
+ ++ L D+ YWIHR LH +W Y +H++HH++ P Y+S H+ + +P +
Sbjct: 155 IALFLLFSDFLIYWIHRALHHRWLYAPLHKLHHKWIVPTPYSSHAFHYLDGYSQSLPYHM 214
Query: 547 GPAIAPGHIMTFWLWITLRQMEAIETHSGYDFPWALTKLVPFYGGAEYHDYHHYVGGQSQ 368
P P + + L + H G F GA +H HH +
Sbjct: 215 FPFFFPLNKYVYLLLFGSVNYWTVLIHDGKYF-----SNNAVVNGAAHHAAHHMYFNYNY 269
Query: 367 SNFASVF 347
F ++F
Sbjct: 270 GQFFTLF 276
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,872,592,117
Number of Sequences: 518415
Number of Extensions: 81872592117
Number of Successful Extensions: 541474699
Number of sequences better than 0.0: 0
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