BLASTX 7.6.2
Query= UN19963 /QuerySize=1427
(1426 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q54CV5|BUB1_DICDI Probable inactive serine/threonine-protein ... 142 9e-033
sp|Q9Z1S0|BUB1B_MOUSE Mitotic checkpoint serine/threonine-protei... 135 9e-031
sp|O60566|BUB1B_HUMAN Mitotic checkpoint serine/threonine-protei... 130 3e-029
sp|O59767|MAD3_SCHPO Mitotic spindle checkpoint component mad3 O... 111 1e-023
sp|O08901|BUB1_MOUSE Mitotic checkpoint serine/threonine-protein... 92 6e-018
>sp|Q54CV5|BUB1_DICDI Probable inactive serine/threonine-protein kinase bub1
OS=Dictyostelium discoideum GN=bub1 PE=3 SV=1
Length = 1306
Score = 142 bits (356), Expect = 9e-033
Identities = 80/226 (35%), Positives = 126/226 (55%), Gaps = 3/226 (1%)
Frame = +2
Query: 158 NEWELFKENVRPLKRGRNVRLLNHALKSHTDHQLRKTLLEKRGKLIEDIDEYDGDDPLSP 337
++WE KEN+ PLK GR+ L AL + +Q + L +++ K I+EY G+DP+
Sbjct: 5 SDWENTKENIVPLKTGRDPTKL--ALFAKQTNQSDEELEKEKQKFEIIINEYQGEDPIDN 62
Query: 338 WIQCIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAEV 517
W++ IKWVQ+++P G L+V+ E+C R F +E+YK+D RYL++W+ YA+ C D
Sbjct: 63 WLKYIKWVQQSYPGGNMKEQLIVLLERCTRLFLSTEKYKNDPRYLRIWITYADMCRDPIE 122
Query: 518 IYKFLEVNEIGKSHALYYIAYALHMEFKSKVKTANEIFNLGISRNAKPMEKLNDAYKKFM 697
++ FLEV IG +L Y A A+ E K + A++ F GI R A+P+E+L + F
Sbjct: 123 VFSFLEVQRIGFYLSLLYEARAIVYENKGNYEQADKSFKQGIERKAQPIERLQQKHLDFE 182
Query: 698 VRTMRRPKTADEEPKDNNDMPSRSFGTVLSRVD-NNNNNTGRQALG 832
R + R K + + ++ ++D N N R ALG
Sbjct: 183 RRLIARLKHQQQHQQLQQQQQQQNDQLEQQQLDENGNTKLTRSALG 228
>sp|Q9Z1S0|BUB1B_MOUSE Mitotic checkpoint serine/threonine-protein kinase BUB1
beta OS=Mus musculus GN=Bub1b PE=1 SV=1
Length = 1052
Score = 135 bits (339), Expect = 9e-031
Identities = 96/342 (28%), Positives = 156/342 (45%), Gaps = 35/342 (10%)
Frame = +2
Query: 155 GNEWELFKENVRPLKRGRNVRLLNHAL-----KSHTDHQLRKTLLEKRGKLIEDIDEYDG 319
G EWEL KEN++PL+ GR + L AL HT Q +K E +I Y G
Sbjct: 12 GAEWELSKENIQPLRHGRVMSTLQGALAKQESAGHTALQQQKRAFE------SEIRFYSG 65
Query: 320 DDPLSPWIQCIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 499
DDPL W + I W ++ +P GG+ S + + E+ + RY +D R+L +W++
Sbjct: 66 DDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLSLWIKLGHL 125
Query: 500 CADAEVIYKFLEVNEIGKSHALYYIAYALHMEFKSKVKTANEIFNLGISRNAKPMEKLND 679
C + +Y +L+ IG S A +YI++A E + K A+ IF GI R A+P+++L
Sbjct: 126 CNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKAEPLDRLQS 185
Query: 680 AYKKFMVRTMRRPKTADEEPKDNNDMPSRSFGTVLSRVDNNNNNTGR------------- 820
+++F R R+ A ++ PS + L+ + + R
Sbjct: 186 QHRQFQSRVSRQAFLALGNEEEEALEPSEPQRSSLAELKSRGKKMARAPISRVGGALKAP 245
Query: 821 -QALGPQAKKPKPNHSSQAPLSIYTDNTQGNHPDSEKSKPEYGSWLMLGGRAERNKENNS 997
Q+ G P+P H ++ ++++ +N E SKP W + R KEN
Sbjct: 246 GQSRGFLNAVPQPVHGNRR-ITVFDENAD-TTSRPELSKPVAQPW--MAPPVPRAKENEL 301
Query: 998 LPGKWKTFKVPQKPIVRTPAASAS------SFEVFVDEEEEE 1105
PG W T + + PA+ S SF +V+E ++
Sbjct: 302 QPGPWSTDRPAGRRPHDNPASVTSIPSVLPSFTPYVEESAQQ 343
>sp|O60566|BUB1B_HUMAN Mitotic checkpoint serine/threonine-protein kinase BUB1
beta OS=Homo sapiens GN=BUB1B PE=1 SV=3
Length = 1050
Score = 130 bits (326), Expect = 3e-029
Identities = 78/248 (31%), Positives = 125/248 (50%), Gaps = 12/248 (4%)
Frame = +2
Query: 155 GNEWELFKENVRPLKRGRNVRLLNHALKSHTDHQLRKTLLEKRGKLIEDIDEYDGDDPLS 334
G+EWEL KENV+PL++GR + L AL + TL +++ +I Y G+DPL
Sbjct: 19 GDEWELSKENVQPLRQGRIMSTLQGALAQ--ESACNNTLQQQKRAFEYEIRFYTGNDPLD 76
Query: 335 PWIQCIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAE 514
W + I W ++ +P GG+ S + + E+ V +RY D R+L +WL+ C +
Sbjct: 77 VWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPL 136
Query: 515 VIYKFLEVNEIGKSHALYYIAYALHMEFKSKVKTANEIFNLGISRNAKPMEKLNDAYKKF 694
+Y +L IG S A +YI++A E + + A+ IF GI + A+P+E+L +++F
Sbjct: 137 DMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQF 196
Query: 695 MVRTMRRPKTA----DEEPKDNNDMPSRSFGTVLSRVDNNNNNTGRQAL---GPQAKKPK 853
R R+ A +EE + +P RS L+ + + T R + G K P
Sbjct: 197 QARVSRQTLLALEKEEEEEVFESSVPQRS---TLAELKSKGKKTARAPIIRVGGALKAPS 253
Query: 854 PNHSSQAP 877
N Q P
Sbjct: 254 QNRGLQNP 261
>sp|O59767|MAD3_SCHPO Mitotic spindle checkpoint component mad3
OS=Schizosaccharomyces pombe GN=mad3 PE=1 SV=2
Length = 310
Score = 111 bits (277), Expect = 1e-023
Identities = 59/193 (30%), Positives = 100/193 (51%), Gaps = 8/193 (4%)
Frame = +2
Query: 149 ETGNEW------ELFKENVRPLKRGRNV-RLLNHALKSHTDHQLRKTLLEKRGKLIEDID 307
+ G W E KEN+ P K G + L + ++HT+ ++ E+ G +
Sbjct: 5 DAGKNWVHMDVIEQSKENIEPRKAGHSASALAKSSSRNHTEKEVAGLQKERMGHERKIET 64
Query: 308 EYDGDDPLSPWIQCIKWVQEAFPPG-GECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWL 484
DDPL WI IKW + FP G + SGL+ + E+C R+F + YKDD+RYL++W+
Sbjct: 65 SESLDDPLQVWIDYIKWTLDNFPQGETKTSGLVTLLERCTREFVRNPLYKDDVRYLRIWM 124
Query: 485 EYAEHCADAEVIYKFLEVNEIGKSHALYYIAYALHMEFKSKVKTANEIFNLGISRNAKPM 664
+Y + + ++ FL + IG+ +++Y YA + E + + A+E++ G AKP
Sbjct: 125 QYVNYIDEPVELFSFLAHHHIGQESSIFYEEYANYFESRGLFQKADEVYQKGKRMKAKPF 184
Query: 665 EKLNDAYKKFMVR 703
+ Y++F R
Sbjct: 185 LRFQQKYQQFTHR 197
>sp|O08901|BUB1_MOUSE Mitotic checkpoint serine/threonine-protein kinase BUB1
OS=Mus musculus GN=Bub1 PE=2 SV=1
Length = 1058
Score = 92 bits (228), Expect = 6e-018
Identities = 45/134 (33%), Positives = 75/134 (55%), Gaps = 2/134 (1%)
Frame = +2
Query: 302 IDEYDGDDPLSPWIQCIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLRYLKVW 481
+ Y G+DPL W IKWV+E FP E L+ + E +++F H + Y +D R++
Sbjct: 15 MQSYTGNDPLGEWESFIKWVEENFPDNKEY--LMTLLEHLMKEFLHKKNYHNDSRFINYC 72
Query: 482 LEYAEHCADAEVIYKFLEVNEIGKSHALYYIAYALHMEFKSKVKTANEIFNLGISRNAKP 661
L++AE+ +D ++FL IG + Y+++A H+E + +++ A+ IF GI A+P
Sbjct: 73 LKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEP 132
Query: 662 MEKLNDAYKKFMVR 703
E L Y+ F R
Sbjct: 133 KELLQQQYRLFQAR 146
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,872,592,117
Number of Sequences: 518415
Number of Extensions: 81872592117
Number of Successful Extensions: 541474699
Number of sequences better than 0.0: 0
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