BLASTX 7.6.2
Query= UN20071 /QuerySize=1040
(1039 letters)
Database: UniProt/TrEMBL;
11,397,958 sequences; 3,661,877,547 total letters
Score E
Sequences producing significant alignments: (bits) Value
tr|Q9SSC0|Q9SSC0_ARATH At1g80190 OS=Arabidopsis thaliana GN=At1g... 366 4e-099
tr|B9MSY7|B9MSY7_POPTR Predicted protein OS=Populus trichocarpa ... 285 7e-075
tr|B9RV31|B9RV31_RICCO Partner of sld5, putative OS=Ricinus comm... 277 2e-072
tr|B6THR1|B6THR1_MAIZE DNA replication complex GINS protein PSF1... 227 2e-057
tr|A2Y5B1|A2Y5B1_ORYSI Putative uncharacterized protein OS=Oryza... 219 5e-055
tr|B9FJD1|B9FJD1_ORYSJ Putative uncharacterized protein OS=Oryza... 219 5e-055
tr|C7J2J4|C7J2J4_ORYSJ Os05g0453800 protein OS=Oryza sativa subs... 191 2e-046
tr|C1EGD1|C1EGD1_9CHLO Predicted protein OS=Micromonas sp. RCC29... 121 1e-025
tr|C1MVM2|C1MVM2_9CHLO Predicted protein OS=Micromonas pusilla C... 118 1e-024
tr|D0MST5|D0MST5_PHYIN DNA replication complex GINS protein PSF1... 105 1e-020
tr|C5YZ66|C5YZ66_SORBI Putative uncharacterized protein Sb09g022... 96 6e-018
tr|B9P6I5|B9P6I5_POPTR Predicted protein OS=Populus trichocarpa ... 92 6e-017
tr|Q00W11|Q00W11_OSTTA Glucose-6-phosphate/phosphate and phospho... 79 1e-012
tr|A5CAG4|A5CAG4_VITVI Putative uncharacterized protein OS=Vitis... 67 2e-009
tr|C5YZ65|C5YZ65_SORBI Putative uncharacterized protein Sb09g022... 67 3e-009
>tr|Q9SSC0|Q9SSC0_ARATH At1g80190 OS=Arabidopsis thaliana GN=At1g80190 PE=2
SV=1
Length = 201
Score = 366 bits (937), Expect = 4e-099
Identities = 174/201 (86%), Positives = 190/201 (94%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MYGRKGYQL+KDFA+GEKGQLKPFNSKLFDETIEEC+QNHHLIQSL+RKMQ+EGL+V NN
Sbjct: 1 MYGRKGYQLIKDFATGEKGQLKPFNSKLFDETIEECDQNHHLIQSLIRKMQQEGLDVQNN 60
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RNADHYGAL+HHL+LIRNKRCLMAYV+NRAEIVRDLAWRVGLEL LP EI EKLTTLEK
Sbjct: 61 RNADHYGALIHHLALIRNKRCLMAYVYNRAEIVRDLAWRVGLELLDLPSEIQEKLTTLEK 120
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
EYFKNHSVA+ SYMGK G+ELNVDMVPPKDPYIKVR++ DID GI+++DK TNFA HSMH
Sbjct: 121 EYFKNHSVALKSYMGKVGIELNVDMVPPKDPYIKVRILDDIDEGIVLSDKTTNFARHSMH 180
Query: 687 FLKRTDAEPYIARGQMEELTG 749
FLKRTDAEPYIARGQMEELTG
Sbjct: 181 FLKRTDAEPYIARGQMEELTG 201
>tr|B9MSY7|B9MSY7_POPTR Predicted protein OS=Populus trichocarpa
GN=POPTRDRAFT_589281 PE=4 SV=1
Length = 201
Score = 285 bits (728), Expect = 7e-075
Identities = 131/201 (65%), Positives = 164/201 (81%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MY RKGY+L+KD A+GEKGQL+PFN LF++ +++C Q++ +Q+L+RKMQ E ++V
Sbjct: 1 MYARKGYELIKDLANGEKGQLQPFNEDLFNQVVDQCSQHYLELQALIRKMQEESVDVRET 60
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RNA+HYGAL+HHLSLIRNKRCLMAYVHNRAEI++ AW+VGLEL LP E+ EKL+ EK
Sbjct: 61 RNAEHYGALIHHLSLIRNKRCLMAYVHNRAEIIQSFAWKVGLELLELPEEVQEKLSLSEK 120
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
YF HS A+ +YM + G++LNVDMVPPKDPYIKVRV+ D+ GI+++DK N A HSMH
Sbjct: 121 NYFGKHSAALQTYMAEVGIDLNVDMVPPKDPYIKVRVLDDMGEGILLSDKTANLARHSMH 180
Query: 687 FLKRTDAEPYIARGQMEELTG 749
FLKRTDAE YIARG MEELTG
Sbjct: 181 FLKRTDAEQYIARGLMEELTG 201
>tr|B9RV31|B9RV31_RICCO Partner of sld5, putative OS=Ricinus communis
GN=RCOM_0898470 PE=4 SV=1
Length = 207
Score = 277 bits (706), Expect = 2e-072
Identities = 131/200 (65%), Positives = 160/200 (80%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MY +KGY+LVK+F+SGEKGQL+ FN LF + I +C Q++ +Q+L+RKMQ E ++V
Sbjct: 7 MYAKKGYELVKEFSSGEKGQLQAFNEDLFHQVIGQCGQHYLELQALIRKMQEESVDVAET 66
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RNADHYGAL HHLSLIRNKRCLMAYV+NRAEI+R+LAW+VG EL LP EI EKL EK
Sbjct: 67 RNADHYGALTHHLSLIRNKRCLMAYVYNRAEIIRNLAWKVGFELLELPEEIQEKLNHSEK 126
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
Y+ HS A+ SYM + G++LNVDMVPPKDPYIKVRV+ D+ GI+++DK N + HSMH
Sbjct: 127 NYYGKHSAALQSYMAEIGIDLNVDMVPPKDPYIKVRVLSDMGEGILLSDKTANLSRHSMH 186
Query: 687 FLKRTDAEPYIARGQMEELT 746
FLKRTDAE YIARG MEELT
Sbjct: 187 FLKRTDAEQYIARGMMEELT 206
>tr|B6THR1|B6THR1_MAIZE DNA replication complex GINS protein PSF1 OS=Zea mays
PE=2 SV=1
Length = 197
Score = 227 bits (577), Expect = 2e-057
Identities = 107/198 (54%), Positives = 145/198 (73%), Gaps = 4/198 (2%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MYGR+ QL+K+ S E GQL PFNS +FD+ I EC +++ QSL+RKM + L++
Sbjct: 1 MYGRRASQLLKEIDSSEAGQLVPFNSDVFDQVIRECNEHNSQFQSLIRKMVEQNLDIETT 60
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RN DHYGA +HHLSL+RNKRCLMAY++NRAE+++ W++G P LP +I EKL EK
Sbjct: 61 RNEDHYGAAIHHLSLLRNKRCLMAYMYNRAEVIQSFRWKIG---PVLPHDIQEKLHFSEK 117
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
EYFKNHS AI SY+ + ++L VDMVPPKDPYI+VRV+ DI + + D + + +S+H
Sbjct: 118 EYFKNHSAAIKSYISEMDIDLTVDMVPPKDPYIQVRVLEDIGE-VSLGDHSVSLTKNSLH 176
Query: 687 FLKRTDAEPYIARGQMEE 740
FL+RTDAE +I++G MEE
Sbjct: 177 FLRRTDAEQFISQGLMEE 194
>tr|A2Y5B1|A2Y5B1_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
indica GN=OsI_20178 PE=4 SV=1
Length = 225
Score = 219 bits (557), Expect = 5e-055
Identities = 106/197 (53%), Positives = 141/197 (71%), Gaps = 4/197 (2%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MYGR+ QL+K+ S E GQL PFNS +FD+ I EC +++ QSL+RKM + L++
Sbjct: 1 MYGRRASQLLKEVDSCEAGQLVPFNSDVFDQVIRECNEHNTQFQSLIRKMVEQNLDIETT 60
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RN DHYGA VHHLSL+RNKRCLMAY++NRAE+++ W++G P LP EI EKL EK
Sbjct: 61 RNDDHYGAAVHHLSLLRNKRCLMAYMYNRAEVIQSFRWKIG---PVLPHEIQEKLHFSEK 117
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
EYFKNHS AI SYM + ++L VDMVPPKDPYI+VRV+ DI + + D + + +S+H
Sbjct: 118 EYFKNHSAAIKSYMSEMDIDLTVDMVPPKDPYIQVRVLEDIGE-VSLGDHSISLTKNSLH 176
Query: 687 FLKRTDAEPYIARGQME 737
FL+RTD E +I++ E
Sbjct: 177 FLRRTDTEQFISQSLCE 193
>tr|B9FJD1|B9FJD1_ORYSJ Putative uncharacterized protein OS=Oryza sativa subsp.
japonica GN=OsJ_18766 PE=4 SV=1
Length = 225
Score = 219 bits (557), Expect = 5e-055
Identities = 106/197 (53%), Positives = 141/197 (71%), Gaps = 4/197 (2%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MYGR+ QL+K+ S E GQL PFNS +FD+ I EC +++ QSL+RKM + L++
Sbjct: 1 MYGRRASQLLKEVDSCEAGQLVPFNSDVFDQVIRECNEHNTQFQSLIRKMVEQNLDIETT 60
Query: 327 RNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEK 506
RN DHYGA VHHLSL+RNKRCLMAY++NRAE+++ W++G P LP EI EKL EK
Sbjct: 61 RNDDHYGAAVHHLSLLRNKRCLMAYMYNRAEVIQSFRWKIG---PVLPHEIQEKLHFSEK 117
Query: 507 EYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMH 686
EYFKNHS AI SYM + ++L VDMVPPKDPYI+VRV+ DI + + D + + +S+H
Sbjct: 118 EYFKNHSAAIKSYMSEMDIDLTVDMVPPKDPYIQVRVLEDIGE-VSLGDHSISLTKNSLH 176
Query: 687 FLKRTDAEPYIARGQME 737
FL+RTD E +I++ E
Sbjct: 177 FLRRTDTEQFISQSLCE 193
>tr|C7J2J4|C7J2J4_ORYSJ Os05g0453800 protein OS=Oryza sativa subsp. japonica
GN=Os05g0453800 PE=4 SV=1
Length = 263
Score = 191 bits (483), Expect = 2e-046
Identities = 91/172 (52%), Positives = 123/172 (71%), Gaps = 4/172 (2%)
Frame = +3
Query: 222 SKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNNRNADHYGALVHHLSLIRNKRCLMAY 401
S +FD+ I EC +++ QSL+RKM + L++ RN DHYGA VHHLSL+RNKRCLMAY
Sbjct: 64 SDVFDQVIRECNEHNTQFQSLIRKMVEQNLDIETTRNDDHYGAAVHHLSLLRNKRCLMAY 123
Query: 402 VHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKEYFKNHSVAITSYMGKAGLELNVDM 581
++NRAE+++ W++G P LP EI EKL EKEYFKNHS AI SYM + ++L VDM
Sbjct: 124 MYNRAEVIQSFRWKIG---PVLPHEIQEKLHFSEKEYFKNHSAAIKSYMSEMDIDLTVDM 180
Query: 582 VPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMHFLKRTDAEPYIARGQME 737
VPPKDPYI+VRV+ DI + + D + + +S+HFL+RTD E +I++ E
Sbjct: 181 VPPKDPYIQVRVLEDIGE-VSLGDHSISLTKNSLHFLRRTDTEQFISQSLCE 231
>tr|C1EGD1|C1EGD1_9CHLO Predicted protein OS=Micromonas sp. RCC299
GN=MICPUN_63601 PE=4 SV=1
Length = 201
Score = 121 bits (303), Expect = 1e-025
Identities = 66/200 (33%), Positives = 112/200 (56%), Gaps = 9/200 (4%)
Frame = +3
Query: 150 YGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNNR 329
+G K LV+D + EKG + P+N +L ++E ++H I L+ +Q +G +
Sbjct: 4 FGDKAATLVQDISQSEKGTIPPYNDELVRAVVDESNEHHRSILRLIGDIQEQGTSLDAAA 63
Query: 330 NADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKE 509
D LVHH +++RNKR L+ Y++ RA+ VR L W VG LP ++ E L+ E+
Sbjct: 64 PEDAAAILVHHQAVLRNKRALLVYLNERADRVRALRWEVGTA---LPEDLGESLSHSERG 120
Query: 510 YFKNHSVAITSYMGK---AGLELNVDMVPPKDPYIKVRVVGDIDRG-IMMNDKATNFAPH 677
YF+ +S + Y G+ G+ + +D PPK+ ++VRV+ +RG ++ D + A +
Sbjct: 121 YFQQYSALVNRYQGRRGGVGINITLDPTPPKEHKVQVRVLQ--ERGELVTRDGTVDLAKN 178
Query: 678 SMHFLKRTDAEPYIARGQME 737
++H L R +A+P I G +E
Sbjct: 179 TVHLLWRDEAQPLITEGVVE 198
>tr|C1MVM2|C1MVM2_9CHLO Predicted protein OS=Micromonas pusilla CCMP1545
GN=MICPUCDRAFT_34219 PE=4 SV=1
Length = 200
Score = 118 bits (294), Expect = 1e-024
Identities = 63/201 (31%), Positives = 106/201 (52%), Gaps = 7/201 (3%)
Frame = +3
Query: 150 YGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNNR 329
+G K LV+D + E+G + F + ++EC+++H I L+ ++ G +
Sbjct: 4 FGEKSAALVRDLSQSERGTIPQFADETVRGVLDECQEHHRSILRLIDDIKERGTSLEAAA 63
Query: 330 NADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKE 509
AD LVHH +++RNKR L+ Y++ R VRDL W VG LP L + E L+ E+
Sbjct: 64 PADAASILVHHQAVLRNKRALLVYMNERMRRVRDLRWEVGAGLPDL---VAESLSHAERT 120
Query: 510 YFKNHSVAITSYMGK---AGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHS 680
+F +S + Y G+ GL + +D PP+D ++VR + D I+ D + +S
Sbjct: 121 FFTEYSALLNKYQGRRHGVGLNVTLDKSPPEDHKVEVRALEDYGE-IVTRDGSVELTRNS 179
Query: 681 MHFLKRTDAEPYIARGQMEEL 743
+H + R + +P I G +E+L
Sbjct: 180 VHLMWRDECQPLIQEGVLEQL 200
>tr|D0MST5|D0MST5_PHYIN DNA replication complex GINS protein PSF1
OS=Phytophthora infestans T30-4 GN=PITG_00071 PE=4 SV=1
Length = 173
Score = 105 bits (261), Expect = 1e-020
Identities = 51/133 (38%), Positives = 78/133 (58%), Gaps = 4/133 (3%)
Frame = +3
Query: 345 GALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKEYFKNH 524
G +V+H L+RNKRCL+AY+H+R E ++ L W G +P ++ + L E ++F +
Sbjct: 43 GLVVNHQCLLRNKRCLIAYLHHRIEKIKALRWETG---TIMPAQLAQNLCQREVQFFNQY 99
Query: 525 SVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMHFLKRTD 704
+T YM L+L+ DM PPKD Y++VRV+ D +M N HS HFL+R D
Sbjct: 100 DQLLTDYMADFELDLSADMKPPKDLYVEVRVLRDCGE-VMTESGLVNLEAHSQHFLRRVD 158
Query: 705 AEPYIARGQMEEL 743
E I +G +E++
Sbjct: 159 VEQLIRQGLLEQI 171
>tr|C5YZ66|C5YZ66_SORBI Putative uncharacterized protein Sb09g022245 (Fragment)
OS=Sorghum bicolor GN=Sb09g022245 PE=4 SV=1
Length = 66
Score = 96 bits (237), Expect = 6e-018
Identities = 44/67 (65%), Positives = 53/67 (79%), Gaps = 3/67 (4%)
Frame = +3
Query: 336 DHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKEYF 515
DHYGA +HHLSL+RNKRCLMAY++NRAE+++ W+VG P LP +I EKL EKEYF
Sbjct: 1 DHYGAAIHHLSLLRNKRCLMAYMYNRAEVIQSFRWKVG---PVLPHDIQEKLHFSEKEYF 57
Query: 516 KNHSVAI 536
KNHS AI
Sbjct: 58 KNHSAAI 64
>tr|B9P6I5|B9P6I5_POPTR Predicted protein OS=Populus trichocarpa
GN=POPTRDRAFT_593186 PE=4 SV=1
Length = 57
Score = 92 bits (228), Expect = 6e-017
Identities = 43/57 (75%), Positives = 48/57 (84%)
Frame = +3
Query: 579 MVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSMHFLKRTDAEPYIARGQMEELTG 749
MVPPKDPYIKVRV+ D+ GI+++DK N A HSMHFLKRTDAE YIARG MEELTG
Sbjct: 1 MVPPKDPYIKVRVLDDMGEGILLSDKTANLARHSMHFLKRTDAEQYIARGLMEELTG 57
>tr|Q00W11|Q00W11_OSTTA Glucose-6-phosphate/phosphate and
phosphoenolpyruvate/phosphate antiporter (ISS) (Fragment)
OS=Ostreococcus tauri GN=Ot14g02010 PE=4 SV=1
Length = 706
Score = 79 bits (192), Expect = 1e-012
Identities = 40/139 (28%), Positives = 75/139 (53%), Gaps = 4/139 (2%)
Frame = +3
Query: 324 NRNADHYGALVHHLSLIRNKRCLMAYVHNRAEIVRDLAWRVGLELPYLPPEILEKLTTLE 503
+ +A G L HH +++RNKR ++ Y++ R V+++ W VG+ LP ++ E
Sbjct: 568 SEDATRVGLLAHHQAILRNKRAMLVYLNERVGRVKEMRWSVGMG---LPESASANMSHGE 624
Query: 504 KEYFKNHSVAITSYMGKAGLELNVDMVPPKDPYIKVRVVGDIDRGIMMNDKATNFAPHSM 683
+ +F ++ + YM L+L +D+ PP++ I+VR + D I D + +++
Sbjct: 625 RTFFTKYAETLNEYMRAVDLDLTLDLEPPQNHKIQVRCLEDHGE-IFTRDGTVDLKKNTV 683
Query: 684 HFLKRTDAEPYIARGQMEE 740
H L R +A+P I G +E+
Sbjct: 684 HLLWREEAQPLIREGVLEQ 702
>tr|A5CAG4|A5CAG4_VITVI Putative uncharacterized protein OS=Vitis vinifera
GN=VITISV_008832 PE=4 SV=1
Length = 137
Score = 67 bits (163), Expect = 2e-009
Identities = 37/80 (46%), Positives = 51/80 (63%), Gaps = 5/80 (6%)
Frame = +3
Query: 342 YGALVHHLSLIRNKRCLMAYV--HNRAEIVRDLAWRVGLELPYLPPEILEKLTTLEKEYF 515
YG L +RN+ ++ +NRAEI+R+L W++G P LP EI EKL+ E+EYF
Sbjct: 30 YGGLGFGNIALRNRALKGKWIRRYNRAEIIRNLRWKIG---PVLPQEIHEKLSYSEEEYF 86
Query: 516 KNHSVAITSYMGKAGLELNV 575
KNHS A+ SYM + L+L V
Sbjct: 87 KNHSTALESYMSELELDLTV 106
>tr|C5YZ65|C5YZ65_SORBI Putative uncharacterized protein Sb09g022240 OS=Sorghum
bicolor GN=Sb09g022240 PE=4 SV=1
Length = 62
Score = 67 bits (162), Expect = 3e-009
Identities = 29/62 (46%), Positives = 42/62 (67%)
Frame = +3
Query: 147 MYGRKGYQLVKDFASGEKGQLKPFNSKLFDETIEECEQNHHLIQSLMRKMQREGLEVPNN 326
MYGR+ QL+K+ S E GQL PFN+ +FD+ I EC +++ QSL+RKM + L++
Sbjct: 1 MYGRRASQLLKEIDSSEAGQLAPFNTDMFDQVIRECSEHNSQFQSLIRKMVEQNLDIETT 60
Query: 327 RN 332
RN
Sbjct: 61 RN 62
Database: UniProt/TrEMBL
Posted date: Sat Aug 07 14:51:12 2010
Number of letters in database: 3,661,877,547
Number of sequences in database: 11,397,958
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,619,371,670,877
Number of Sequences: 11397958
Number of Extensions: 1619371670877
Number of Successful Extensions: 591400999
Number of sequences better than 0.0: 0
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