BLASTX 7.6.2
Query= UN20530 /QuerySize=1567
(1566 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P92947|MDARP_ARATH Monodehydroascorbate reductase, chloroplas... 570 9e-162
sp|Q42711|MDARS_CUCSA Monodehydroascorbate reductase, seedling i... 240 2e-062
sp|Q40977|MDAR_PEA Monodehydroascorbate reductase OS=Pisum sativ... 235 1e-060
sp|Q43497|MDAR_SOLLC Monodehydroascorbate reductase OS=Solanum l... 230 3e-059
sp|Q9LK94|MDAR2_ARATH Probable monodehydroascorbate reductase, c... 230 4e-059
sp|Q9LFA3|MDAR3_ARATH Probable monodehydroascorbate reductase, c... 226 4e-058
sp|Q93WJ8|MDAR4_ARATH Probable monodehydroascorbate reductase, c... 214 2e-054
sp|Q9SR59|MDAR1_ARATH Probable monodehydroascorbate reductase, c... 198 1e-049
sp|P16640|CAMA_PSEPU Putidaredoxin reductase OS=Pseudomonas puti... 95 1e-018
sp|P43494|THCD_RHOER Rhodocoxin reductase OS=Rhodococcus erythro... 91 3e-017
>sp|P92947|MDARP_ARATH Monodehydroascorbate reductase, chloroplastic
OS=Arabidopsis thaliana GN=At1g63940 PE=2 SV=3
Length = 493
Score = 570 bits (1469), Expect = 9e-162
Identities = 282/304 (92%), Positives = 296/304 (97%), Gaps = 1/304 (0%)
Frame = +3
Query: 54 AVRRAMALASTTLPAKTGLSLWCPASPCSLARRLPARFSPIGSRIASRGIVTASFANENR 233
AVRR MALASTTLP K+GLSLWCP+SP SLARR PARFSPIGSRIASR +VTASFANENR
Sbjct: 3 AVRRVMALASTTLPTKSGLSLWCPSSP-SLARRFPARFSPIGSRIASRSLVTASFANENR 61
Query: 234 EFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKKPARLP 413
EFVI+GGGNAAGYAARTFVENGMADGRLC+VTKEAYAPYERPALTKAYLFPPEKKPARLP
Sbjct: 62 EFVIVGGGNAAGYAARTFVENGMADGRLCIVTKEAYAPYERPALTKAYLFPPEKKPARLP 121
Query: 414 GFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGC 593
GFHTCVGGGGERQTPDWYKEKGIE+IYEDPV GADFEKQTLTT+ GKQLKYGSLIIATGC
Sbjct: 122 GFHTCVGGGGERQTPDWYKEKGIEVIYEDPVAGADFEKQTLTTDAGKQLKYGSLIIATGC 181
Query: 594 TASRFSDKIGGNLPGVHYIREVADADSLISSLGKSKKVVIVGGGYIGMEVAAAAVAWNLD 773
TASRF DKIGG+LPGVHYIREVADADSLI+SLGK+KK+VIVGGGYIGMEVAAAAVAWNLD
Sbjct: 182 TASRFPDKIGGHLPGVHYIREVADADSLIASLGKAKKIVIVGGGYIGMEVAAAAVAWNLD 241
Query: 774 TTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAVKLADGS 953
TTIVFPEDQLLQRLFTPSLAQ+YE+LYRQNGVKFVKGASINNLEAGSDGRV+AVKLADGS
Sbjct: 242 TTIVFPEDQLLQRLFTPSLAQKYEELYRQNGVKFVKGASINNLEAGSDGRVSAVKLADGS 301
Query: 954 TIEA 965
TIEA
Sbjct: 302 TIEA 305
Score = 159 bits (401), Expect = 6e-038
Identities = 85/119 (71%), Positives = 96/119 (80%), Gaps = 6/119 (5%)
Frame = +2
Query: 974 EHCVKSLLTAHTDTYDYLPYFYSRVFEYEGSSRKVWWQFLGIMWEKQLK*QLRSQD---- 1141
+HCVKSLLTAHTDTYDYLPYFYSRVFEYEGS RKVWWQF G + + ++ + D
Sbjct: 377 QHCVKSLLTAHTDTYDYLPYFYSRVFEYEGSPRKVWWQFFGDNVGETV--EVGNFDPKIA 434
Query: 1142 RYLLDDLAGESVLVESGSPEEFQLLPKLARSQPIVDKAKLAGASSVEEALEIAQAALQS 1318
+ ++ + VLVESGSPEEFQLLPKLARSQP+VDKAKLA ASSVEEALEIAQAALQS
Sbjct: 435 TFWIESGRLKGVLVESGSPEEFQLLPKLARSQPLVDKAKLASASSVEEALEIAQAALQS 493
>sp|Q42711|MDARS_CUCSA Monodehydroascorbate reductase, seedling isozyme
OS=Cucumis sativus PE=2 SV=1
Length = 434
Score = 240 bits (612), Expect = 2e-062
Identities = 125/253 (49%), Positives = 164/253 (64%), Gaps = 6/253 (2%)
Frame = +3
Query: 219 ANENREFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKK 398
A+E ++VI+GGG AAGYAAR FV+ G+ G L +++KEA APYERPAL+KAYLFP +
Sbjct: 2 ADETFKYVILGGGVAAGYAAREFVKQGLNPGELAIISKEAVAPYERPALSKAYLFP--ES 59
Query: 399 PARLPGFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLI 578
PARLPGFH CVG GGER PDWYKEKGIE+I + AD + L + GK Y +LI
Sbjct: 60 PARLPGFHVCVGSGGERLLPDWYKEKGIELILSTEIVEADLPAKRLRSAHGKIYNYQTLI 119
Query: 579 IATGCTASRFSD--KIGGNLPGVHYIREVADADSLISSL--GKSKKVVIVGGGYIGMEVA 746
IATG T + SD G + + Y+RE+ DAD L+ ++ ++ KVV+VGGGYIG+E+
Sbjct: 120 IATGSTVIKLSDFGVQGADAKNIFYLREIDDADQLVEAIKAKENGKVVVVGGGYIGLELG 179
Query: 747 AAAVAWNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRV 926
AA N D ++V+PE + RLFTP +A YE Y Q G+ +KG ++G V
Sbjct: 180 AALRINNFDVSMVYPEPWCMPRLFTPEIAAFYEGYYAQKGITIIKGTVAVGFTVDTNGEV 239
Query: 927 TAVKLADGSTIEA 965
VKL DG +EA
Sbjct: 240 KEVKLKDGRVLEA 252
>sp|Q40977|MDAR_PEA Monodehydroascorbate reductase OS=Pisum sativum PE=1 SV=1
Length = 433
Score = 235 bits (597), Expect = 1e-060
Identities = 120/248 (48%), Positives = 158/248 (63%), Gaps = 6/248 (2%)
Frame = +3
Query: 234 EFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKKPARLP 413
+++IIGGG +AGYAAR FV+ G+ G L +++KEA APYERPAL+KAYLFP + PARLP
Sbjct: 6 KYIIIGGGVSAGYAAREFVKQGVHPGELAIISKEAVAPYERPALSKAYLFP--ESPARLP 63
Query: 414 GFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGC 593
GFHTCVG GGER P+WY EKGI++ + AD + L + G+ Y +L+IATG
Sbjct: 64 GFHTCVGSGGERLLPEWYSEKGIQLYLSTEIVSADLAAKFLKSANGEHFDYQTLVIATGS 123
Query: 594 TASRFSD--KIGGNLPGVHYIREVADADSLISSLGKSK--KVVIVGGGYIGMEVAAAAVA 761
R +D IG N + Y+REV DAD L ++ + K K V+VGGGYIG+E++A
Sbjct: 124 AVIRLTDFGVIGANAKNIFYLREVDDADKLYEAIKRKKNAKRVVVGGGYIGLELSAVLKL 183
Query: 762 WNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAVKL 941
+LD T+V+PE + RLFT +A YE Y G+ +KG A SDG V VKL
Sbjct: 184 NDLDVTMVYPEPWCMPRLFTSEIAAFYEGYYANKGINIIKGTVAVGFTANSDGEVKEVKL 243
Query: 942 ADGSTIEA 965
DG +EA
Sbjct: 244 KDGRVLEA 251
>sp|Q43497|MDAR_SOLLC Monodehydroascorbate reductase OS=Solanum lycopersicum
GN=AFRR PE=1 SV=1
Length = 433
Score = 230 bits (585), Expect = 3e-059
Identities = 117/253 (46%), Positives = 161/253 (63%), Gaps = 6/253 (2%)
Frame = +3
Query: 219 ANENREFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKK 398
A ++ ++VI+GGG +AGYAAR F + G+ G L +++KEA APYERPAL+KAYLFP +
Sbjct: 2 AEKSFKYVIVGGGVSAGYAAREFAKQGVKPGELAIISKEAVAPYERPALSKAYLFP--EG 59
Query: 399 PARLPGFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLI 578
ARLPGFH CVG GGERQ P+WY EKGI +I + AD +TL + G+ KY +L+
Sbjct: 60 AARLPGFHVCVGSGGERQLPEWYAEKGISLILSTEIVKADLASKTLVSAAGESFKYQTLV 119
Query: 579 IATGCTASRFSD--KIGGNLPGVHYIREVADADSLISSL--GKSKKVVIVGGGYIGMEVA 746
IATG T + SD G + + Y+RE+ DAD L+ +L K+ K V+VGGGYIG+E++
Sbjct: 120 IATGTTVLKLSDFGVQGADSKNIFYLREIDDADQLVEALKAKKNGKAVVVGGGYIGLELS 179
Query: 747 AAAVAWNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRV 926
A N++ +V+PE + RLFT +A YE Y+ GV +KG + +G V
Sbjct: 180 AVLRLNNIEVNMVYPEPWCMPRLFTEGIAAFYEGYYKNKGVNIIKGTVAVGFDTHPNGEV 239
Query: 927 TAVKLADGSTIEA 965
VKL DG +EA
Sbjct: 240 KEVKLKDGRVLEA 252
>sp|Q9LK94|MDAR2_ARATH Probable monodehydroascorbate reductase, cytoplasmic
isoform 2 OS=Arabidopsis thaliana GN=At3g27820 PE=2 SV=1
Length = 488
Score = 230 bits (584), Expect = 4e-059
Identities = 117/247 (47%), Positives = 161/247 (65%), Gaps = 6/247 (2%)
Frame = +3
Query: 237 FVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKKPARLPG 416
+VI+GGG AAGYAA F G++DG LC++++E APYERPAL+K +L P + PARLP
Sbjct: 7 YVILGGGVAAGYAALEFTRRGVSDGELCIISEEPVAPYERPALSKGFLLP--EAPARLPS 64
Query: 417 FHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGCT 596
FHTCVG E+ TP WYK+ GIE++ V D ++TL ++TG+ + Y LIIATG
Sbjct: 65 FHTCVGANDEKLTPKWYKDHGIELVLGTRVKSVDVRRKTLLSSTGETISYKFLIIATGAR 124
Query: 597 ASRFSD--KIGGNLPGVHYIREVADADSLISSLGKSK--KVVIVGGGYIGMEVAAAAVAW 764
A + + G + V Y+R++ADA+ L + + S V++GGGYIGME AA+ V
Sbjct: 125 ALKLEEFGVEGSDAENVCYLRDLADANRLATVIQSSSNGNAVVIGGGYIGMECAASLVIN 184
Query: 765 NLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAVKLA 944
++ T+VFPE + RLFTP +A YED YR GVKF+KG + + E S+ +VTAV L
Sbjct: 185 KINVTMVFPEAHCMARLFTPKIASLYEDYYRAKGVKFIKGTVLTSFEFDSNKKVTAVNLK 244
Query: 945 DGSTIEA 965
DGS + A
Sbjct: 245 DGSHLPA 251
>sp|Q9LFA3|MDAR3_ARATH Probable monodehydroascorbate reductase, cytoplasmic
isoform 3 OS=Arabidopsis thaliana GN=At3g52880 PE=1 SV=1
Length = 434
Score = 226 bits (575), Expect = 4e-058
Identities = 119/253 (47%), Positives = 161/253 (63%), Gaps = 6/253 (2%)
Frame = +3
Query: 219 ANENREFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKK 398
A ++ +++I+GGG +AGYAA+ F G+ G L V++KEA APYERPAL+K YLFP +
Sbjct: 2 AEKSFKYIILGGGVSAGYAAKEFANQGVQPGELAVISKEAVAPYERPALSKGYLFP--EG 59
Query: 399 PARLPGFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLI 578
ARLPGFH CVG GGE+ P+ YK+KGIE+I + AD ++L + TG KY +LI
Sbjct: 60 AARLPGFHCCVGSGGEKLLPESYKQKGIELILSTEIVKADLSAKSLVSATGDVFKYQTLI 119
Query: 579 IATGCTASRFSD--KIGGNLPGVHYIREVADADSLISSLGKSK--KVVIVGGGYIGMEVA 746
IATG T R +D G + + Y+RE+ DAD L+ ++ K K V+VGGGYIG+E++
Sbjct: 120 IATGSTVLRLTDFGVKGADSKNILYLREIDDADKLVEAIKAKKGGKAVVVGGGYIGLELS 179
Query: 747 AAAVAWNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRV 926
A NLD T+VFPE + RLFT +A YE Y GVK +KG + A +G V
Sbjct: 180 AVLRINNLDVTMVFPEPWCMPRLFTADIAAFYETYYTNKGVKIIKGTVASGFTAQPNGEV 239
Query: 927 TAVKLADGSTIEA 965
V+L DG T+EA
Sbjct: 240 KEVQLKDGRTLEA 252
>sp|Q93WJ8|MDAR4_ARATH Probable monodehydroascorbate reductase, cytoplasmic
isoform 4 OS=Arabidopsis thaliana GN=At5g03630 PE=1 SV=1
Length = 435
Score = 214 bits (544), Expect = 2e-054
Identities = 114/248 (45%), Positives = 151/248 (60%), Gaps = 6/248 (2%)
Frame = +3
Query: 234 EFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKKPARLP 413
++VI+GGG AAGYAAR F G+ G L ++++E PYERPAL+K Y+ K A LP
Sbjct: 8 KYVIVGGGVAAGYAAREFFNQGVKPGELAIISREQVPPYERPALSKGYIHLENK--ATLP 65
Query: 414 GFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGC 593
F+ G GGERQ P WYKEKGIE+I + AD +TL + TG+ KY +L+ ATG
Sbjct: 66 NFYVAAGIGGERQFPQWYKEKGIELILGTEIVKADLAAKTLVSGTGQVFKYQTLLAATGS 125
Query: 594 TASRFSD--KIGGNLPGVHYIREVADADSLISSLGKSK--KVVIVGGGYIGMEVAAAAVA 761
+ R SD G + + Y+RE+ DAD L ++ + K V+VGGGYIG+E+ AA A
Sbjct: 126 SVIRLSDFGVPGADAKNIFYLRELEDADYLAYAMETKEKGKAVVVGGGYIGLELGAALKA 185
Query: 762 WNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAVKL 941
NLD T+V+PE + RLFT +A YE Y G+ VKG + S+G VT VKL
Sbjct: 186 NNLDVTMVYPEPWCMPRLFTAGIASFYEGYYANKGINIVKGTVASGFTTNSNGEVTEVKL 245
Query: 942 ADGSTIEA 965
DG T+EA
Sbjct: 246 KDGRTLEA 253
>sp|Q9SR59|MDAR1_ARATH Probable monodehydroascorbate reductase, cytoplasmic
isoform 1 OS=Arabidopsis thaliana GN=At3g09940 PE=2 SV=1
Length = 441
Score = 198 bits (502), Expect = 1e-049
Identities = 107/250 (42%), Positives = 150/250 (60%), Gaps = 9/250 (3%)
Frame = +3
Query: 234 EFVIIGGGNAAGYAARTFVENGMADGRLCVVTKEAYAPYERPALTKAYLFPPEKKPARLP 413
++VIIGGG A GYAAR F G+ G L +++KE P+ERP LTK Y+ E P L
Sbjct: 8 KYVIIGGGVAGGYAAREFSNQGLKPGELAIISKEPVPPFERPELTKVYI-DLEVNPT-LA 65
Query: 414 GFHTCVGGGGERQTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGC 593
+ C G G +Q P+WYKEKGI++I + AD +TL ++ GK KY +L+IATG
Sbjct: 66 NIYVCAGTGEAKQYPNWYKEKGIDLIVGTEIVKADLASKTLVSDDGKIYKYQTLLIATGS 125
Query: 594 TASRFSDKIG---GNLPGVHYIREVADADSLISSLG---KSKKVVIVGGGYIGMEVAAAA 755
T R S+ IG ++ + Y+RE+ D+D L ++ + K VI+GGG++G+E+++A
Sbjct: 126 TNIRLSE-IGVQEADVKNIFYLREIEDSDELALAMELYVQRGKAVIIGGGFLGLEISSAL 184
Query: 756 VAWNLDTTIVFPEDQLLQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAV 935
A N + T+VFPE L+ R FT +A YE Y G+K +KG SDG VT V
Sbjct: 185 RANNHEVTMVFPEPWLVHRFFTAEIASFYESYYANKGIKIIKGTVATGFSTNSDGEVTEV 244
Query: 936 KLADGSTIEA 965
KL DG T+EA
Sbjct: 245 KLEDGRTLEA 254
>sp|P16640|CAMA_PSEPU Putidaredoxin reductase OS=Pseudomonas putida GN=camA PE=1
SV=1
Length = 422
Score = 95 bits (234), Expect = 1e-018
Identities = 55/175 (31%), Positives = 94/175 (53%), Gaps = 3/175 (1%)
Frame = +3
Query: 450 QTPDWYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGCTASRFSDKIG-- 623
+TPD Y + I+++ VT + ++Q + + G+ L Y L++ATG G
Sbjct: 65 RTPDAYAAQNIQLLGGTQVTAINRDRQQVILSDGRALDYDRLVLATGGRPRPLPVASGAV 124
Query: 624 GNLPGVHYIREVADADSLISSLGKSKKVVIVGGGYIGMEVAAAAVAWNLDTTIVFPEDQL 803
G Y+R + DA+ + L ++V++GGGYIG+EVAA A+ N+ T++ ++
Sbjct: 125 GKANNFRYLRTLEDAECIRRQLIADNRLVVIGGGYIGLEVAATAIKANMHVTLLDTAARV 184
Query: 804 LQRLFTPSLAQRYEDLYRQNGVKFVKGASINNLEAGSD-GRVTAVKLADGSTIEA 965
L+R+ P ++ YE L+R+ GV G + E +D +VTAV DG+ + A
Sbjct: 185 LERVTAPPVSAFYEHLHREAGVDIRTGTQVCGFEMSTDQQKVTAVLCEDGTRLPA 239
>sp|P43494|THCD_RHOER Rhodocoxin reductase OS=Rhodococcus erythropolis GN=thcD
PE=1 SV=2
Length = 427
Score = 91 bits (223), Expect = 3e-017
Identities = 53/168 (31%), Positives = 85/168 (50%), Gaps = 2/168 (1%)
Frame = +3
Query: 462 WYKEKGIEMIYEDPVTGADFEKQTLTTNTGKQLKYGSLIIATGCTASRFSDKIGGNLPGV 641
++ + I + PV D + Q + ++Y LI+ATG +R G NLPGV
Sbjct: 65 YFDDHRITLTCGKPVVRIDRDAQRVELIDATAIEYDHLILATG-ARNRLLPVPGANLPGV 123
Query: 642 HYIREVADADSLISSLGKSKKVVIVGGGYIGMEVAAAAVAWNLDTTIVFPEDQLLQRLFT 821
HY+R +A+SL SS+ +V++G G+IG+EVAAAA LD T+V D+ + R +
Sbjct: 124 HYLRTAGEAESLTSSMASCSSLVVIGAGFIGLEVAAAARKKGLDVTVVEAMDRPMARALS 183
Query: 822 PSLAQRYEDLYRQNGVKFVKGASINNLEAGSDGRVTAVKLADGSTIEA 965
++ + + ++GV + + A +DGR V G I A
Sbjct: 184 SVMSGYFSTAHTEHGVHMRLSTGVKTINA-ADGRAAGVTTNSGDVIHA 230
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,924,390,942
Number of Sequences: 518415
Number of Extensions: 86924390942
Number of Successful Extensions: 587655121
Number of sequences better than 0.0: 0
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