BLASTX 7.6.2
Query= UN22014 /QuerySize=1024
(1023 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q03529|SCS7_YEAST Ceramide very long chain fatty acid hydroxy... 176 4e-043
sp|O13846|SCS7_SCHPO Ceramide very long chain fatty acid hydroxy... 157 1e-037
sp|Q2LAM0|FA2H_RAT Fatty acid 2-hydroxylase OS=Rattus norvegicus... 133 2e-030
sp|Q4R4P4|FA2H_MACFA Fatty acid 2-hydroxylase OS=Macaca fascicul... 132 5e-030
sp|Q7L5A8|FA2H_HUMAN Fatty acid 2-hydroxylase OS=Homo sapiens GN... 131 1e-029
sp|Q5MPP0|FA2H_MOUSE Fatty acid 2-hydroxylase OS=Mus musculus GN... 128 7e-029
>sp|Q03529|SCS7_YEAST Ceramide very long chain fatty acid hydroxylase SCS7
OS=Saccharomyces cerevisiae GN=SCS7 PE=1 SV=1
Length = 384
Score = 176 bits (444), Expect = 4e-043
Identities = 99/236 (41%), Positives = 130/236 (55%), Gaps = 9/236 (3%)
Frame = +3
Query: 153 VDLDKPLVFQV--GHLGK--YQEWIHQPIVCVEGPRFFQSDFWEFLTRTVW*AIPTTWLP 320
+DL++PL+ Q+ K Y + IH+P +G +F E LT+T W +P WLP
Sbjct: 148 LDLNRPLLMQILRSDFKKDFYVDQIHRPRHYGKGSAPLFGNFLEPLTKTAWWVVPVAWLP 207
Query: 321 VVCYVLSISAARGLTFPQMGLIVAFGVFTWTLLEYTLHRFLFHIE---TKSYWANTAHYL 491
VV Y + + A + + + GVF WTL+EY LHRFLFH + +S A H+L
Sbjct: 208 VVVYHMGV-ALKNMNQLFACFLFCVGVFVWTLIEYGLHRFLFHFDDWLPESNIAFATHFL 266
Query: 492 LHGCHHKHPQDGLCLVFPPTATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTH 671
LHGCHH P D LV PPT IL P +KL+ + A A G LFGYV YD H
Sbjct: 267 LHGCHHYLPMDKYRLVMPPTLFVILCAPFYKLVFALLPLYWAYAGFAGGLFGYVCYDECH 326
Query: 672 FYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITFSFWDRVFGTLPGTKAAQKKL 839
F+LHH + P + LKKYHL HH++ G+G+T FWD VFGT G A K+
Sbjct: 327 FFLHHSK-LPPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSKM 381
>sp|O13846|SCS7_SCHPO Ceramide very long chain fatty acid hydroxylase-like
protein C19G12.08 OS=Schizosaccharomyces pombe GN=SPAC19G12.08 PE=2
SV=1
Length = 347
Score = 157 bits (396), Expect = 1e-037
Identities = 89/228 (39%), Positives = 120/228 (52%), Gaps = 13/228 (5%)
Frame = +3
Query: 153 VDLDKPLVFQV--GHLGK--YQEWIHQP-IVCVEGPRFFQSDFWEFLTRTVW*AIPTTWL 317
+DL KPL+ Q+ G++ K Y + +H+P G +F E LT+T W IP W+
Sbjct: 107 LDLKKPLLPQILFGNIKKDVYLDQVHRPRHYRGSGSAPLFGNFLEPLTKTPWYMIPLIWV 166
Query: 318 PVVCYVLSISAARGLTFPQMGLIVAFGVFTWTLLEYTLHRFLFHIETKSYWAN-----TA 482
P V Y + A G+ F G+FTWTL+EYT+HRFLFH++ Y + T
Sbjct: 167 PCVTYGF-LYACTGIPFSVAITFFIIGLFTWTLVEYTMHRFLFHLD--EYTPDHPIFLTM 223
Query: 483 HYLLHGCHHKHPQDGLCLVFPPTATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYD 662
H+ HGCHH P D LV PP I P + + L+ A G + GYV YD
Sbjct: 224 HFAFHGCHHFLPADKYRLVMPPALFLIFATPWYHFIQLVLPHYIGVAGFSGAILGYVFYD 283
Query: 663 CTHFYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITFSFWDRVFGT 806
TH++LHH + LK +HL+HH++ YGIT FWDRVFGT
Sbjct: 284 LTHYFLHHRRMPNAYLTDLKTWHLDHHYKDYKSAYGITSWFWDRVFGT 331
>sp|Q2LAM0|FA2H_RAT Fatty acid 2-hydroxylase OS=Rattus norvegicus GN=Fa2h PE=1
SV=2
Length = 372
Score = 133 bits (334), Expect = 2e-030
Identities = 65/156 (41%), Positives = 95/156 (60%), Gaps = 4/156 (2%)
Frame = +3
Query: 375 MGLIVAFGVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLCLVFPP 548
+GL V G+ WTL+EY +HRFLFH++ + S++ H+++HG HHK P DG LVFPP
Sbjct: 216 IGLFV-LGMLIWTLVEYLIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPP 274
Query: 549 TATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKK 725
++++ + L LI A A + G L GYV+YD TH+YLH G P + ++ + +K
Sbjct: 275 VPASVVVAFFYVFLRLILPEAVAGILFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKA 334
Query: 726 YHLNHHFRIQDKGYGITFSFWDRVFGTLPGTKAAQK 833
+H+ HHF Q G+GI+ WD F TL +A K
Sbjct: 335 HHVKHHFEYQKSGFGISTKLWDYFFHTLIPEEADPK 370
Score = 71 bits (173), Expect = 1e-011
Identities = 39/82 (47%), Positives = 51/82 (62%), Gaps = 4/82 (4%)
Frame = +3
Query: 153 VDLDKPLVFQVGHLG-KYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVW*AIPTTWLPVVC 329
VD KPL++QVGHLG KY EW+HQP+ R F SD E ++TVW ++P W+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--ARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 181
Query: 330 YVLSISAARGLTFPQMGLIVAF 395
Y LS S R LT + L +F
Sbjct: 182 Y-LSWSYYRTLTQDNIRLFASF 202
>sp|Q4R4P4|FA2H_MACFA Fatty acid 2-hydroxylase OS=Macaca fascicularis GN=FA2H
PE=2 SV=1
Length = 372
Score = 132 bits (331), Expect = 5e-030
Identities = 61/141 (43%), Positives = 87/141 (61%), Gaps = 3/141 (2%)
Frame = +3
Query: 396 GVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLCLVFPPTATAILL 569
G+F W+L+EY +HRFLFH++ + SY+ H+++HG HHK P DG LVFPP ++++
Sbjct: 222 GIFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Query: 570 VPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKKYHLNHHF 746
+ L LI A + G L GYV+YD TH+YLH G P ++ + LK +H+ HHF
Sbjct: 282 GVFYLCLQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHRGSYLYNLKAHHVKHHF 341
Query: 747 RIQDKGYGITFSFWDRVFGTL 809
Q G+GI+ WD F TL
Sbjct: 342 AHQKSGFGISTKLWDYCFHTL 362
Score = 69 bits (168), Expect = 4e-011
Identities = 43/86 (50%), Positives = 54/86 (62%), Gaps = 7/86 (8%)
Frame = +3
Query: 153 VDLDKPLVFQVGHLG-KYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVW*AIPTTWLPVVC 329
VD KPL++QVGHLG KY EW+HQP+ R F SD E L++TVW ++P W+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--TRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVL 181
Query: 330 YVLSISAARGLTFPQMGLIVAFGVFT 407
Y LS S R TF Q G + F FT
Sbjct: 182 Y-LSWSYYR--TFAQ-GNVRLFTSFT 203
>sp|Q7L5A8|FA2H_HUMAN Fatty acid 2-hydroxylase OS=Homo sapiens GN=FA2H PE=1
SV=1
Length = 372
Score = 131 bits (328), Expect = 1e-029
Identities = 61/145 (42%), Positives = 88/145 (60%), Gaps = 3/145 (2%)
Frame = +3
Query: 396 GVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLCLVFPPTATAILL 569
G F W+L+EY +HRFLFH++ + SY+ H+++HG HHK P DG LVFPP ++++
Sbjct: 222 GTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVI 281
Query: 570 VPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKKYHLNHHF 746
+ + LI A + G L GYV+YD TH+YLH G P + ++ + LK +H+ HHF
Sbjct: 282 GVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHF 341
Query: 747 RIQDKGYGITFSFWDRVFGTLPGTK 821
Q G+GI+ WD F TL K
Sbjct: 342 AHQKSGFGISTKLWDYCFHTLTPEK 366
>sp|Q5MPP0|FA2H_MOUSE Fatty acid 2-hydroxylase OS=Mus musculus GN=Fa2h PE=1
SV=1
Length = 372
Score = 128 bits (321), Expect = 7e-029
Identities = 62/156 (39%), Positives = 92/156 (58%), Gaps = 4/156 (2%)
Frame = +3
Query: 375 MGLIVAFGVFTWTLLEYTLHRFLFHIE--TKSYWANTAHYLLHGCHHKHPQDGLCLVFPP 548
+GL V G+ WT +EY +HRFLFH++ + S++ H+++HG HHK P DG LVFPP
Sbjct: 216 IGLFV-LGMLFWTFVEYVIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPP 274
Query: 549 TATAILLVPLWKLLHLIATPATAPAMLGGILFGYVMYDCTHFYLHHGQPKEPTFKH-LKK 725
++++ + L LI + G L GYV+YD TH+YLH G P + ++ + +K
Sbjct: 275 VPASLVIAFFYVFLRLILPETVGGIIFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKA 334
Query: 726 YHLNHHFRIQDKGYGITFSFWDRVFGTLPGTKAAQK 833
+H+ HHF Q G+GI+ WD F TL +A K
Sbjct: 335 HHVKHHFEYQKSGFGISTKLWDYFFHTLIPEEAHPK 370
Score = 69 bits (167), Expect = 5e-011
Identities = 37/72 (51%), Positives = 47/72 (65%), Gaps = 4/72 (5%)
Frame = +3
Query: 153 VDLDKPLVFQVGHLG-KYQEWIHQPIVCVEGPRFFQSDFWEFLTRTVW*AIPTTWLPVVC 329
VD KPL++QVGHLG KY EW+HQP+ R F SD E ++TVW ++P W+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--ARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 181
Query: 330 YVLSISAARGLT 365
Y LS S R LT
Sbjct: 182 Y-LSWSYYRTLT 192
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,892,683,958
Number of Sequences: 518415
Number of Extensions: 92892683958
Number of Successful Extensions: 611291351
Number of sequences better than 0.0: 0
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