BLASTX 7.6.2
Query= UN22940 /QuerySize=1261
(1260 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 190 3e-047
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 183 4e-045
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 168 1e-040
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 158 1e-037
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 156 3e-037
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 146 3e-034
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 144 2e-033
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 143 3e-033
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 142 6e-033
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 142 8e-033
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 142 8e-033
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 141 1e-032
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 140 2e-032
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 115 1e-024
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 114 2e-024
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 112 9e-024
sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegi... 96 6e-019
sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus... 94 2e-018
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 92 7e-018
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 92 9e-018
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 190 bits (480), Expect = 3e-047
Identities = 94/264 (35%), Positives = 143/264 (54%), Gaps = 14/264 (5%)
Frame = -1
Query: 1113 NLTTLETLWFEYSATKSDYYLYCHNILFLFLIF---SLVPLPLVFLELARSAWFDRYKIQ 943
+L +E WF++ + +L + +F S +P F + +YKIQ
Sbjct: 5 SLKFIEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIP----FFLCDFMPFLQKYKIQ 60
Query: 942 PKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLVVY 763
P KN + F C V+ + V P+ + P+I+ I + + PLPS + +
Sbjct: 61 PTKKNEWKTQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACC 120
Query: 762 FLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAI 583
FL+ED+ YWVHR H + Y+ IH +HH++ AP G A YAH E ++LGV T +GP +
Sbjct: 121 FLIEDFYFYWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFL 180
Query: 582 APGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH--YVGGQSQSN 409
+ T W+W+ R + +E HSGYDFPW TK IPF+GG+ +HD+HH +VG N
Sbjct: 181 FSRDLFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHETFVG-----N 235
Query: 408 FASVFTYCDYIYGTDKGYRFQKKL 337
++S FTY D I+GT Y +K++
Sbjct: 236 YSSTFTYLDKIFGTSDKYYSRKQI 259
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 183 bits (462), Expect = 4e-045
Identities = 95/265 (35%), Positives = 143/265 (53%), Gaps = 7/265 (2%)
Frame = -1
Query: 1113 NLTTLETLWFEYSATKSDYYLYCHNILFLFLIFSLVPLPLVFLELARSAWFDRYKIQPKV 934
+L +E WF++ + +L + +F + FL +F +YKIQ
Sbjct: 5 SLKFIEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCF-IPFLIADFIPFFRKYKIQQTK 63
Query: 933 KNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLVVYFLV 754
+N + C V+ +L+ P+ + P+I+ I + + PLPS + LV F++
Sbjct: 64 ENDWKSQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFII 123
Query: 753 EDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPG 574
ED+ YW HR H Y+ IH +HH+Y +P G A YAH E ++LGV T +GP +
Sbjct: 124 EDFYFYWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSR 183
Query: 573 HMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVF 394
+ T W+W+ +R + +E HSGYDFPW+ T IPF+GGA +HDYHH V N+AS F
Sbjct: 184 DLFTLWVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEV---FIGNYASTF 240
Query: 393 TYCDYIYGTD-KGY--RFQKKLLQQ 328
TY D I+GT K Y R +KK + +
Sbjct: 241 TYLDKIFGTSGKSYYSRIEKKSINK 265
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 168 bits (423), Expect = 1e-040
Identities = 93/282 (32%), Positives = 154/282 (54%), Gaps = 22/282 (7%)
Frame = -1
Query: 1110 LTTLETLWFEYSATKSDYYLYCHNI----LFLFLIFSLVPLP--LVFLELARSAWFDRYK 949
L +E LW Y Y + +++ L FL+ L+ + ++ + +F R+K
Sbjct: 27 LNFVEQLWLAY------YKWFDNDVVATGLMSFLLHELIYFGRCIPWMIIDAMPYFRRWK 80
Query: 948 IQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLV 769
IQPK + ++ + C R V+ V P + P + + +P P ++ Q+
Sbjct: 81 IQPKKVPTLAEQWECTRLVLLSHFTVELPQIWLFDPMCATFGLSTSVPFPPVTKMIWQIT 140
Query: 768 VYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGP 589
++F +ED +YW HR FH Y IH +HH Y+AP G +A YAH E++LLG T P
Sbjct: 141 LFFFLEDTWHYWAHRLFHYGIFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVP 200
Query: 588 AI-----APGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHHYVGG 424
+ H++T ++WI LR +A+++H+GYDFPW+L KF+P + GA++HDYHH
Sbjct: 201 LMWCYFTHDLHLVTMYIWITLRLFQAVDSHAGYDFPWSLNKFLPIWAGADHHDYHHMA-- 258
Query: 423 QSQSNFASVFTYCDYIYGTDKGY-RFQ-KKLLQQIKEESKKS 304
+ NF+S F + D + TD+ Y +F+ ++L + + ESKK+
Sbjct: 259 -FKDNFSSSFRWWDAVLKTDQNYHQFKARRLAAKYEAESKKA 299
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 158 bits (397), Expect = 1e-037
Identities = 90/275 (32%), Positives = 142/275 (51%), Gaps = 10/275 (3%)
Frame = -1
Query: 1110 LTTLETLWFEYSATKSDYYLYCHNILFLFLIFSLVPLPLVFLELARSAWFDRYKIQPKVK 931
L +E W + + ++ L + FL F L + + + +F R+K+QP
Sbjct: 33 LNFMEKYWAAWYSYMNNDVLATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKI 92
Query: 930 NSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLVVYFLVE 751
S + C + V+ LV +P + + I +P PS +A ++ ++F++E
Sbjct: 93 PSAKEQLYCLKSVLLSHFLVEAIPIWTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLE 152
Query: 750 DYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAI---- 583
D +YW HR FH Y+ IH HH Y AP G +A YAH AE L LG T P +
Sbjct: 153 DTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMY 212
Query: 582 -APGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHD-YHHYVGGQSQSN 409
H+ T +WI LR +A+++HSGYDFPW+L K +PF+ GAE+HD +HHY G N
Sbjct: 213 TGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHHHYFIG----N 268
Query: 408 FASVFTYCDYIYGTDKGYRFQKKLLQQIKEESKKS 304
+AS F + DY T+ G + +++K+ ++ +
Sbjct: 269 YASSFRWWDYCLDTESGPEAKASREERMKKRAENN 303
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 156 bits (394), Expect = 3e-037
Identities = 95/285 (33%), Positives = 145/285 (50%), Gaps = 22/285 (7%)
Frame = -1
Query: 1113 NLTTLETLWFEYSATKSDYYLYCHNILF-----LFLIFSLVPLPLVFLELARSAWFDRYK 949
NL E LW Y ++ L+ +LF +F +P ++ R +F ++K
Sbjct: 32 NLNVFEKLWGSYYYYMAN-DLFATGLLFFLTHEIFYFGRCLPWAII----DRIPYFRKWK 86
Query: 948 IQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLV 769
IQ + S + + C + V+ LV +P Q I I +P P ++ Q
Sbjct: 87 IQDEKIPSDKEQWECLKSVLTSHFLVEAFPIWFFHPLCQKIGISYQVPFPKITDMLIQWA 146
Query: 768 VYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGP 589
V+F++ED +YW HR H Y+ IH HH Y AP G AA YAH EV LLG+ T P
Sbjct: 147 VFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGTVGIP 206
Query: 588 AI-----APGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH--YV 430
+ H+ T +WI LR +A++ HSGY+FPW+L F+PF+ GA++HD HH ++
Sbjct: 207 IVWCLITGNLHLFTVSIWIILRLFQAVDAHSGYEFPWSLHNFLPFWAGADHHDEHHHYFI 266
Query: 429 GGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQIKEESKKSNKQ 295
GG S S F + D+I T+ G + +K ++K+ +K K+
Sbjct: 267 GGYSSS-----FRWWDFILDTEAGPKAKKGREDKVKQNVEKLQKK 306
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 146 bits (368), Expect = 3e-034
Identities = 78/238 (32%), Positives = 125/238 (52%), Gaps = 13/238 (5%)
Frame = -1
Query: 1029 LFLIFSLVPLPLVFLELARSAWFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLV 850
++ +FSL F+ R +YKIQ +F ++C + ++ + PL
Sbjct: 61 IYFLFSLPGFLFQFIPFMR-----KYKIQKDKPETFEGQWKCLKGILFNHFFIQLPLICG 115
Query: 849 SYPSIQMIEIRSGLP-LPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHE 673
+Y + I +P + A+ + ++ED +Y++HR H K Y+ IH +HHE
Sbjct: 116 TYYFTEFFNIPYDWERMPRWYFTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHE 175
Query: 672 YTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPW 493
+ AP G A YAH E L+LG F+G + H+I W W+ +R +E I+ HSGYD P
Sbjct: 176 FQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTMRLLETIDVHSGYDIPL 235
Query: 492 TLTKFIPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQI 325
+IPFY GA +HD+HH ++G N+AS FT+ D I+GTD Y + ++++
Sbjct: 236 NPLNYIPFYTGARHHDFHHMNFIG-----NYASTFTWWDRIFGTDVQYHAYTEKMKKL 288
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 144 bits (361), Expect = 2e-033
Identities = 76/228 (33%), Positives = 119/228 (52%), Gaps = 13/228 (5%)
Frame = -1
Query: 1029 LFLIFSLVPLPLVFLELARSAWFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLV 850
++ +FSL F+ R +YKIQ +F ++C + ++ + PL
Sbjct: 61 IYFLFSLPGFLFQFIPYMR-----KYKIQKDKPETFEGQWKCLKKILFNHFFIQLPLICG 115
Query: 849 SYPSIQMIEIRSGLP-LPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHE 673
+Y + I +P + A+ + ++ED +Y++HR H K Y+ IH +HHE
Sbjct: 116 TYYFTEFFNIPYDWERMPRWYLTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHE 175
Query: 672 YTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPW 493
+ AP G A YAH E L+LG F+G + H+I W W+ +R +E I+ HSGYD P
Sbjct: 176 FQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPL 235
Query: 492 TLTKFIPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGY 355
+PFY GA +HD+HH ++G N+AS FT+ D ++GTD Y
Sbjct: 236 NPLNLVPFYTGARHHDFHHMNFIG-----NYASTFTWWDKLFGTDAQY 278
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 143 bits (360), Expect = 3e-033
Identities = 70/207 (33%), Positives = 112/207 (54%), Gaps = 8/207 (3%)
Frame = -1
Query: 966 WFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEI-RSGLPLPSFG 790
+ +YKIQP ++ ++C++ ++ + PL +Y + I +P +
Sbjct: 77 YMQKYKIQPDKPETWEKQWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWY 136
Query: 789 EIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLG 610
+ AQ ++ED +Y++HR H K Y+ IH +HHE+ +P G A YAH E L+LG
Sbjct: 137 VLLAQCFGCAVIEDAWHYFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILG 196
Query: 609 VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH-- 436
F+G + H++ W W+ R +E I+ HSGYD P +PFY GA +HD+HH
Sbjct: 197 AGFFIGIVVFCNHVVLLWAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMN 256
Query: 435 YVGGQSQSNFASVFTYCDYIYGTDKGY 355
++G N+AS FT+ D I+GTD +
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQF 278
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 142 bits (357), Expect = 6e-033
Identities = 73/214 (34%), Positives = 117/214 (54%), Gaps = 10/214 (4%)
Frame = -1
Query: 966 WFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLP-LPSFG 790
+ +YKIQ ++ + ++C++ ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 789 EIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLG 610
+ A+ ++ED +Y++HR H K Y+ IH +HHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 609 VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH-- 436
F+G + H+I W W+ +R +E I+ HSGYD P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 435 YVGGQSQSNFASVFTYCDYIYGTDKGYR--FQKK 340
++G N+AS FT+ D I+GTD Y ++KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYHAYYEKK 285
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 142 bits (356), Expect = 8e-033
Identities = 74/220 (33%), Positives = 118/220 (53%), Gaps = 16/220 (7%)
Frame = -1
Query: 966 WFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLP-LPSFG 790
+ +YKIQ ++ + ++C++ ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 789 EIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLG 610
+ A+ ++ED +Y++HR H K Y+ IH +HHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 609 VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH-- 436
F+G + H+I W W+ +R +E I+ HSGYD P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 435 YVGGQSQSNFASVFTYCDYIYGTDKGY--------RFQKK 340
++G N+AS FT+ D I+GTD Y +F+KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYNAYNEKRKKFEKK 291
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 142 bits (356), Expect = 8e-033
Identities = 74/220 (33%), Positives = 118/220 (53%), Gaps = 16/220 (7%)
Frame = -1
Query: 966 WFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLP-LPSFG 790
+ +YKIQ ++ + ++C++ ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 789 EIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLG 610
+ A+ ++ED +Y++HR H K Y+ IH +HHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 609 VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH-- 436
F+G + H+I W W+ +R +E I+ HSGYD P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN 256
Query: 435 YVGGQSQSNFASVFTYCDYIYGTDKGY--------RFQKK 340
++G N+AS FT+ D I+GTD Y +F+KK
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDSQYNAYNEKRKKFEKK 291
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 141 bits (355), Expect = 1e-032
Identities = 75/226 (33%), Positives = 118/226 (52%), Gaps = 10/226 (4%)
Frame = -1
Query: 1023 LIFSLVPLPLVFLELARSAWFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSY 844
LI+ L LP + + +YKIQP ++ ++C++ ++ + PL +Y
Sbjct: 60 LIYFLFCLPGFIFQFL--PFMQKYKIQPDKPETWEKQWKCFKMLLFNHFCIQLPLICGTY 117
Query: 843 PSIQMIEIRSGL-PLPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYT 667
+ I +P + + AQ ++ED +Y++HR H + Y+ IH +HH++T
Sbjct: 118 YFTEFFSIPYDWDTMPRWPFLLAQCFGCAVIEDTWHYFLHRALHHRRIYKYIHKVHHDFT 177
Query: 666 APIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTL 487
+P G A YAH E L+LG F+G + HMI W W+ R +E I+ HSGYD P
Sbjct: 178 SPFGMQAEYAHPLETLILGAGFFIGTMVFCNHMILLWAWVTFRLLETIDVHSGYDIPLNP 237
Query: 486 TKFIPFYGGAEYHDYHH--YVGGQSQSNFASVFTYCDYIYGTDKGY 355
IPFY GA +HD+HH +VG N+ S FT+ D ++ TD +
Sbjct: 238 LHLIPFYAGARFHDFHHMNFVG-----NYGSTFTWWDRLFDTDSQF 278
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 140 bits (352), Expect = 2e-032
Identities = 73/207 (35%), Positives = 112/207 (54%), Gaps = 8/207 (3%)
Frame = -1
Query: 966 WFDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEI-RSGLPLPSFG 790
+ +YKIQ ++ + ++C++ ++ + PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQFPLICGTYYFTEYFSIPYDWETMPRWY 136
Query: 789 EIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLG 610
A+ ++ED +Y++HR H K Y+ IH IHHE+ AP G A YAH E L+LG
Sbjct: 137 IALARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLILG 196
Query: 609 VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHH-- 436
F+G + H+I W W+ +R IE I+ HSGYD P IPFY G+ +HD+HH
Sbjct: 197 TGFFIGIMLLCDHVILLWAWVTVRLIETIDVHSGYDIPLNPLHLIPFYAGSRHHDFHHMN 256
Query: 435 YVGGQSQSNFASVFTYCDYIYGTDKGY 355
++G N+AS FT+ D I+GTD Y
Sbjct: 257 FIG-----NYASTFTWWDRIFGTDAQY 278
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 115 bits (286), Expect = 1e-024
Identities = 69/196 (35%), Positives = 102/196 (52%), Gaps = 10/196 (5%)
Frame = -1
Query: 897 DVMKMFLLVVGPLQLV-SYPS--IQMIEI-RSGLPL--PSFGEIAAQLVVYFLVEDYTNY 736
D+MK L + G QL+ YP +Q+I + + LP+ P+ E+ +QL ++FL D+T +
Sbjct: 87 DLMKKSLKLQGWNQLLWIYPMALVQLIWVPDTELPILAPTVFEMVSQLAIFFLAFDFTYF 146
Query: 735 WVHRFFHS-KWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITF 559
W H F H KW Y H +HH Y++P +A + H E+ + P I P H +T+
Sbjct: 147 WFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVATFITTVPWIFPTHCLTY 206
Query: 558 WLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDY 379
WLW + Q + E H GYDFP+ L + FY GA HD HH + + F F Y D
Sbjct: 207 WLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHL---RPLTCFQPWFNYLDR 263
Query: 378 IYGTDKGYRFQKKLLQ 331
+ G Y KK+ +
Sbjct: 264 LMGYHITYEDLKKMTE 279
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 114 bits (283), Expect = 2e-024
Identities = 71/234 (30%), Positives = 115/234 (49%), Gaps = 18/234 (7%)
Frame = -1
Query: 1017 FSLVPLPLVFLELARSAW--FDRYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSY 844
+ ++ LP + ++ W RYKIQP + + + C + +L+V P + +
Sbjct: 49 YFVLVLPYLSCDILGRKWPAIYRYKIQPDKLPTTAMLLHCSGVTLYNHILLVIPAAVAQW 108
Query: 843 ---PSIQMIEIRSGLPLPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSK--WGYEKIHHIH 679
P I + E L L G + L+++ L Y++ F H K W Y H IH
Sbjct: 109 MWRPPIPLPEQAPTL-LELVGGVTGNLLLFDL-----QYFIWHFLHHKIRWLYVTFHAIH 162
Query: 678 HEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDF 499
H Y+AP A E++ +G T L P + H++T W+++ + ++E H GYDF
Sbjct: 163 HNYSAPFALATQCLGGWELVTVGFWTTLNPVLLRCHLLTTWMFMVVHVYVSVEDHCGYDF 222
Query: 498 PWTLTKFIPF--YGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQK 343
PW+ ++ IPF YGG HD HH + +NFA F++ D ++GT +RF K
Sbjct: 223 PWSTSRLIPFGVYGGPSKHDVHHQ---KPNTNFAPHFSHWDKMFGTHADFRFSK 273
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 112 bits (278), Expect = 9e-024
Identities = 67/196 (34%), Positives = 102/196 (52%), Gaps = 10/196 (5%)
Frame = -1
Query: 897 DVMKMFLLVVGPLQLV-SYPS--IQMIEI-RSGLPL--PSFGEIAAQLVVYFLVEDYTNY 736
D++K L + G QL+ YP +Q+I + + LP+ P+ E+ +QL ++FL D+T +
Sbjct: 87 DLIKKSLKLQGWNQLLWIYPMALVQLIWVPDTELPILAPTVFEMLSQLAIFFLAFDFTYF 146
Query: 735 WVHRFFHS-KWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPAIAPGHMITF 559
W H H KW Y H +HH Y++P +A + H E+ +G P I P H +T+
Sbjct: 147 WFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFELFFVGTFITTIPWIFPTHCLTY 206
Query: 558 WLWIALRQIEAIETHSGYDFPWTLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDY 379
W+W + Q + E H GYDFP+ L + FY GA HD HH + + F F Y D
Sbjct: 207 WIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHDMHHL---RPLTCFQPWFNYLDR 263
Query: 378 IYGTDKGYRFQKKLLQ 331
+ G Y KK+ +
Sbjct: 264 LMGYHITYADLKKMTE 279
>sp|Q4QQV7|CH25H_RAT Cholesterol 25-hydroxylase OS=Rattus norvegicus GN=Ch25h
PE=2 SV=1
Length = 298
Score = 96 bits (236), Expect = 6e-019
Identities = 70/265 (26%), Positives = 113/265 (42%), Gaps = 13/265 (4%)
Frame = -1
Query: 1113 NLTTLETLWFEYSATKSDYYLYCHNILFLFLIFSLVPLPLVFLELARSAW---FDRYKIQ 943
N L+ LW ++ ++F + LP V L++ W RYKI
Sbjct: 16 NQLLLQPLWDSIRTGEASARSPFFPVIFSIFTYLGFCLPFVVLDVL-CPWVPILRRYKIH 74
Query: 942 PKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLPLPSFGEIAAQLVVY 763
P S + C + L+ V P+ L+ + + R P ++ + +++
Sbjct: 75 PDFSPSVRQLLPCLGLTLYQHLVFVFPVTLMHWARSPALLPREA---PELSQLLSHVLIC 131
Query: 762 FLVEDYTNYWVHRFFHS-KWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGVPTFLGPA 586
L+ D + H H W Y H +HH+ ++ A Y E+L L L A
Sbjct: 132 LLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVA 191
Query: 585 IAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPF--YGGAEYHDYHHYVGGQSQS 412
+ H +T ++ + ++E HSGYDFPW+ + +PF YGG +HD HH Q
Sbjct: 192 MLQCHPLTILVFHVVNIWLSVEDHSGYDFPWSTHRLVPFGWYGGVAHHDLHH---SQFNC 248
Query: 411 NFASVFTYCDYIYGTDKGYRFQKKL 337
NFA FT+ D + GT + K+L
Sbjct: 249 NFAPYFTHWDKMLGTLRCAPHSKRL 273
>sp|Q9Z0F5|CH25H_MOUSE Cholesterol 25-hydroxylase OS=Mus musculus GN=Ch25h PE=1
SV=1
Length = 298
Score = 94 bits (231), Expect = 2e-018
Identities = 59/202 (29%), Positives = 94/202 (46%), Gaps = 13/202 (6%)
Frame = -1
Query: 957 RYKIQPKVKNSFSDMFRCYRDVMKMFLLVVGPLQLVSYPSIQMIEIRSGLP--LPSFGEI 784
RYKI P S + C + L+ V P+ L+ + + + LP P ++
Sbjct: 70 RYKIHPDFSPSVKQLLPCLGLTLYQHLVFVFPVTLLHW-----VRSPALLPQEAPELVQL 124
Query: 783 AAQLVVYFLVEDYTNYWVHRFFHS-KWGYEKIHHIHHEYTAPIGYAAPYAHWAEVLLLGV 607
+ +++ L+ D + H H W Y H +HH+ ++ A Y + E+L L
Sbjct: 125 LSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSFWELLSLTF 184
Query: 606 PTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFIPF--YGGAEYHDYHHY 433
L A+ H +T + + + ++E HSGYDFPW+ + +PF YGG +HD HH
Sbjct: 185 FDVLNVAVLRCHPLTIFTFHVINIWLSVEDHSGYDFPWSTHRLVPFGWYGGVAHHDMHH- 243
Query: 432 VGGQSQSNFASVFTYCDYIYGT 367
Q NFA FT+ D + GT
Sbjct: 244 --SQFNCNFAPYFTHWDKMLGT 263
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 92 bits (227), Expect = 7e-018
Identities = 61/193 (31%), Positives = 93/193 (48%), Gaps = 13/193 (6%)
Frame = -1
Query: 1002 LPLVFLELARSAWFDRYKIQPKVKNSFSD---MFRCYRDVMKMFLLVVGPLQLVSYPSIQ 832
L LV + + RY+IQ KN D + + R V+ +V P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVG-KNEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLK 155
Query: 831 MIEIRSGLPLPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGY 652
LP+F +L ++ L+E+ Y+ HR H Y+KIH HHE+TAPIG
Sbjct: 156 WWGDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGV 215
Query: 651 AAPYAHWAE-VLLLGVPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFI 475
+ YAH E V+ +P +GP + H+ + +W +L I +H GY +
Sbjct: 216 ISLYAHPIEHVVSNMLPAIVGPLVMGSHLSSITMWFSLALIITTISHCGY--------HL 267
Query: 474 PFYGGAEYHDYHH 436
PF E+HDYHH
Sbjct: 268 PFLPSPEFHDYHH 280
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 92 bits (226), Expect = 9e-018
Identities = 59/193 (30%), Positives = 92/193 (47%), Gaps = 13/193 (6%)
Frame = -1
Query: 1002 LPLVFLELARSAWFDRYKIQPKVKNSFSD---MFRCYRDVMKMFLLVVGPLQLVSYPSIQ 832
L LV + + RY+IQ KN D + + R V+ ++ P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVG-KNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLK 155
Query: 831 MIEIRSGLPLPSFGEIAAQLVVYFLVEDYTNYWVHRFFHSKWGYEKIHHIHHEYTAPIGY 652
LP+F +L ++ L+E+ Y+ HR H Y+KIH HHE+TAPIG
Sbjct: 156 WWRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGV 215
Query: 651 AAPYAHWAEVLLLG-VPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWTLTKFI 475
+ YAH E + +P +GP + H+ + +W +L I +H GY +
Sbjct: 216 ISLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGY--------HL 267
Query: 474 PFYGGAEYHDYHH 436
PF E+HDYHH
Sbjct: 268 PFLPSPEFHDYHH 280
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,819,846,109
Number of Sequences: 518415
Number of Extensions: 95819846109
Number of Successful Extensions: 619733697
Number of sequences better than 0.0: 0
|
