BLASTX 7.6.2
Query= UN26215 /QuerySize=2126
(2125 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P59015|VPS18_DANRE Vacuolar protein sorting-associated protei... 234 3e-060
sp|Q9P253|VPS18_HUMAN Vacuolar protein sorting-associated protei... 217 3e-055
sp|Q8R307|VPS18_MOUSE Vacuolar protein sorting-associated protei... 216 5e-055
sp|Q24314|VPS18_DROME Vacuolar protein sorting-associated protei... 145 2e-033
sp|O74925|PEP3_SCHPO Vacuolar membrane protein pep3 OS=Schizosac... 129 1e-028
sp|P27801|PEP3_YEAST Vacuolar membrane protein PEP3 OS=Saccharom... 93 8e-018
>sp|P59015|VPS18_DANRE Vacuolar protein sorting-associated protein 18 homolog
OS=Danio rerio GN=vps18 PE=2 SV=2
Length = 974
Score = 234 bits (595), Expect = 3e-060
Identities = 127/265 (47%), Positives = 180/265 (67%), Gaps = 13/265 (4%)
Frame = +3
Query: 342 ETVEAWMASKN-LNPRRLITAMMRYSSEPHAKNETHEVIKYLEFCVHSLHNEDPGIHNLL 518
+ V+AW+ N L+P+ LI A++ Y S+ + + +E I+Y+EFCV+ L ++ IHN L
Sbjct: 609 KVVDAWIQMGNRLDPKNLIPALVNY-SQMGSMQQINETIRYMEFCVYELDVKEEAIHNYL 667
Query: 519 LSLYAKQEDDSALLRFLQCKFGKGRENGPEFFYDPKYALRLCLKEKRTRACVHIYSMMSM 698
LSLYAK + D ALL +L+ + + + YD KYALRLC + +ACV +Y +M +
Sbjct: 668 LSLYAKHKPD-ALLWYLE----QAGTHVSDIHYDLKYALRLCSEHGYLQACVLVYKIMEL 722
Query: 699 HEEAVVLALQIDPELAMAEAGKVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFL 878
+EEAV LAL++D +LA + A EDDE+LRKKLWL +A+HVV++EK +++KA+ L
Sbjct: 723 YEEAVDLALKVDVDLAKSCADLPEDDEELRKKLWLKIARHVVQEEK-----DVKKAMNCL 777
Query: 879 KETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRND 1058
+ LLKIEDILPFFPDF ID FKEAICSSLE+YNK IE+LK+EM +AT A IR D
Sbjct: 778 SSCN-LLKIEDILPFFPDFVTIDHFKEAICSSLEEYNKHIEELKQEMEEATESAKRIRED 836
Query: 1059 ISALTQRYAVIDREEECGVCKRKIL 1133
I + +Y V++ +E+C C +L
Sbjct: 837 IQEMRNKYGVVESQEKCATCDFPLL 861
Score = 74 bits (181), Expect = 3e-012
Identities = 40/111 (36%), Positives = 64/111 (57%), Gaps = 5/111 (4%)
Frame = +3
Query: 1191 PFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQKQLTLLGSETRRDINGNRSDEPVT 1370
PFY+F CGH FH CL+ V + + +LQK+L ++T + + R ++ V+
Sbjct: 864 PFYLFLCGHMFHYDCLLQEVIPHLSVYKQNKLDELQKKLAAT-TQTTKARHKPREEDTVS 922
Query: 1371 ---STTTADKLRSELDDAIASECPFCGELMINEITLPFIKPEE-TQHSASW 1511
+ ++++S++DD IA EC +CGELMI I PFI P++ Q +SW
Sbjct: 923 LGKGQGSREQIKSDIDDIIACECVYCGELMIKSIDKPFIDPQKFDQEMSSW 973
>sp|Q9P253|VPS18_HUMAN Vacuolar protein sorting-associated protein 18 homolog
OS=Homo sapiens GN=VPS18 PE=1 SV=2
Length = 973
Score = 217 bits (552), Expect = 3e-055
Identities = 123/283 (43%), Positives = 175/283 (61%), Gaps = 14/283 (4%)
Frame = +3
Query: 288 PEIFSFR*TSELIMLDAYETVEAWM-ASKNLNPRRLITAMMRYSSEPHAKNETHEVIKYL 464
P++F ++ + LI + V+AW+ L+ R+LI A++ YS + + + I+Y+
Sbjct: 592 PQLF-YKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQ-QVSQAIRYM 649
Query: 465 EFCVHSLHNEDPGIHNLLLSLYAKQEDDSALLRFLQCKFGKGRENGPEFFYDPKYALRLC 644
EFCV+ L + IHN LLSLYA+ DS L Q R + YD KYALRLC
Sbjct: 650 EFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVH-----YDLKYALRLC 704
Query: 645 LKEKRTRACVHIYSMMSMHEEAVVLALQIDPELAMAEAGKVEDDEDLRKKLWLMVAKHVV 824
+ RACVH+Y ++ ++EEAV LALQ+D +LA A E+DE+LRKKLWL +A+HVV
Sbjct: 705 AEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVV 764
Query: 825 KQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQ 1004
++E E+++ A+A L LLKIED+LPFFPDF ID FKEAICSSL+ YN I++
Sbjct: 765 QEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQE 818
Query: 1005 LKEEMNDATRGADNIRNDISALTQRYAVIDREEECGVCKRKIL 1133
L+ EM +AT A IR D+ L RY ++ +++C C +L
Sbjct: 819 LQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLL 861
Score = 73 bits (177), Expect = 8e-012
Identities = 39/113 (34%), Positives = 62/113 (54%), Gaps = 10/113 (8%)
Frame = +3
Query: 1191 PFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQKQL-----TLLGSETRRDINGNRS 1355
PFY+F CGH FHA CL+ V + + +LQ++L GS ++ G +
Sbjct: 864 PFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAA 923
Query: 1356 DEPVTSTTTADKLRSELDDAIASECPFCGELMINEITLPFIKPEE-TQHSASW 1511
T+ + ++L+++LD+ +A+EC +CGELMI I PFI P+ + SW
Sbjct: 924 ----TAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSW 972
>sp|Q8R307|VPS18_MOUSE Vacuolar protein sorting-associated protein 18 homolog
OS=Mus musculus GN=Vps18 PE=1 SV=2
Length = 973
Score = 216 bits (550), Expect = 5e-055
Identities = 123/283 (43%), Positives = 176/283 (62%), Gaps = 14/283 (4%)
Frame = +3
Query: 288 PEIFSFR*TSELIMLDAYETVEAWM-ASKNLNPRRLITAMMRYSSEPHAKNETHEVIKYL 464
P++F ++ + LI + V+AW+ L+ R+LI A++ YS A+ + + I+Y+
Sbjct: 592 PQLF-YKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEAQ-QVSQAIRYM 649
Query: 465 EFCVHSLHNEDPGIHNLLLSLYAKQEDDSALLRFLQCKFGKGRENGPEFFYDPKYALRLC 644
EFCV+ L + IHN LLSLYA+ + S L Q R + YD KYALRLC
Sbjct: 650 EFCVNVLGETEQAIHNYLLSLYARGQPASLLAYLEQAGASPHRVH-----YDLKYALRLC 704
Query: 645 LKEKRTRACVHIYSMMSMHEEAVVLALQIDPELAMAEAGKVEDDEDLRKKLWLMVAKHVV 824
+ RACVH+Y ++ ++EEAV LALQ+D +LA A E+DE+LRKKLWL +A+HVV
Sbjct: 705 AEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVV 764
Query: 825 KQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQ 1004
++E E+++ A+A L LLKIED+LPFFPDF ID FKEAICSSL+ YN I++
Sbjct: 765 QEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQE 818
Query: 1005 LKEEMNDATRGADNIRNDISALTQRYAVIDREEECGVCKRKIL 1133
L+ EM +AT A IR D+ L RY ++ +++C C +L
Sbjct: 819 LQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTCDFPLL 861
>sp|Q24314|VPS18_DROME Vacuolar protein sorting-associated protein 18 homolog
OS=Drosophila melanogaster GN=dor PE=1 SV=3
Length = 1002
Score = 145 bits (364), Expect = 2e-033
Identities = 84/234 (35%), Positives = 134/234 (57%), Gaps = 12/234 (5%)
Frame = +3
Query: 432 KNETHEVIKYLEFCVHSLHNEDPGIHNLLLSLYAKQEDDSALLRFLQCKFGKGRENGPEF 611
+ + + +YLEF ++ L+ + IHN LL LYA+ E L+++L+ +GR+
Sbjct: 672 REQREQTQRYLEFAIYKLNTTNDAIHNFLLHLYAEHE-PKLLMKYLEI---QGRDES-LV 726
Query: 612 FYDPKYALRLCLKEKRTRACVHIYSMMSMHEEAVVLALQIDPELAMAEAGKVEDDEDLRK 791
YD YA ++C A V + M+ AV LAL D +LA A + D + +R+
Sbjct: 727 HYDIYYAHKVCTDLDVKEARVFLECMLRKWISAVDLALTFDMKLAKETASRPSDSK-IRR 785
Query: 792 KLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICS 971
KLWL +A H +K +++KA+ LKE D LL+IED+LPFF DF ID+FKEAIC
Sbjct: 786 KLWLRIAYHDIK-----GTNDVKKALNLLKECD-LLRIEDLLPFFADFEKIDNFKEAICD 839
Query: 972 SLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDREEECGVCKRKIL 1133
+L DYN++I++L+ EM + T D + ++ L Q ++ ++ C +C+ +L
Sbjct: 840 ALRDYNQRIQELQREMAETTEQTDRVTAELQQLRQHSLTVESQDTCEICEMMLL 893
>sp|O74925|PEP3_SCHPO Vacuolar membrane protein pep3 OS=Schizosaccharomyces
pombe GN=pep3 PE=2 SV=1
Length = 900
Score = 129 bits (322), Expect = 1e-028
Identities = 77/268 (28%), Positives = 137/268 (51%), Gaps = 16/268 (5%)
Frame = +3
Query: 342 ETVEAWMASKNLNPRRLITAMMRYSSEPHAKNETHEVIKYLEFCVHSLHNEDPGIHNLLL 521
ETV W +L+ LI +++ Y+ H E + I+YL + L DP IHN L
Sbjct: 592 ETVSIWERQTDLDVHALIPSLLSYNQRSHVPVEENAAIRYLRYVTGVLGCVDPSIHNTLF 651
Query: 522 SLYA--KQEDDSALLRFLQCKFGKGRENGPEFFYDPKYALRLCLKEKRTRACVHIYSMMS 695
+YA ++S L+ +++ + G YD +RLCL+ R+ V I +M
Sbjct: 652 CIYACHSSSNESYLMNYIE-------QQGNHPLYDMDLGIRLCLQFNCRRSAVKILVLMK 704
Query: 696 MHEEAVVLALQIDP-ELAMAEAGKVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIA 872
++ + V LAL+ D ELA A E+D L+K LW +AK++ +K+ I++ +
Sbjct: 705 LYSQGVELALEADDCELAATIANIPEEDVVLKKTLWQTIAKYMF-----SKKSGIKETLR 759
Query: 873 FLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIR 1052
FL+ ++ +L++ +++ P+ +DD + +C L+ K+IEQL E+ A+ A I+
Sbjct: 760 FLENSE-VLQLPELIRLLPEDIKLDDLSDNVCDELDHCMKRIEQLDFEIGQASEVAHEIQ 818
Query: 1053 NDISALTQRYAVIDREEECGVCKRKILT 1136
+ + RY V++ E C C + + +
Sbjct: 819 TNAENMRNRYIVLEPNESCWHCNQPLFS 846
>sp|P27801|PEP3_YEAST Vacuolar membrane protein PEP3 OS=Saccharomyces cerevisiae
GN=PEP3 PE=1 SV=1
Length = 918
Score = 93 bits (229), Expect = 8e-018
Identities = 54/184 (29%), Positives = 102/184 (55%), Gaps = 9/184 (4%)
Frame = +3
Query: 576 KFGKGRENGPEFFYDPKYALRLCLKEKRTRACVHIYSMMSMHEEAVVLALQ---IDPELA 746
KF K EN YD + LRL LK K+T+ + + + +++ E+A+ LAL+ ID
Sbjct: 652 KFMKSNENK----YDLNFQLRLSLKFKKTKTSIFLLTRLNLFEDAIDLALKNNLIDDCKV 707
Query: 747 MAEAGKVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFF 926
+ + +D LRK+LWL +AKH++ K + + + I L +++ +L I+D+LPFF
Sbjct: 708 IVNDEILIEDYKLRKRLWLKIAKHLLLSMKDIDIKQLIRTI--LNDSNEILTIKDLLPFF 765
Query: 927 PDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDREEE 1106
++ I + KE + LE++N ++ ++ E++ ++ I +IS + Y +++ +
Sbjct: 766 NEYTTIANLKEELIKFLENHNMKMNEISEDIINSKNLKVEINTEISKFNEIYRILEPGKS 825
Query: 1107 CGVC 1118
C C
Sbjct: 826 CDEC 829
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,753,696,895
Number of Sequences: 518415
Number of Extensions: 109753696895
Number of Successful Extensions: 688305551
Number of sequences better than 0.0: 0
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