BLASTX 7.6.2
Query= UN28703 /QuerySize=762
(761 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SP... 103 2e-021
sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sp... 101 6e-021
sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE... 100 2e-020
sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SP... 96 2e-019
sp|O14159|LCB4_SCHPO Sphingoid long chain base kinase 4 OS=Schiz... 96 2e-019
sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE... 93 2e-018
sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus GN... 93 2e-018
sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sp... 87 8e-017
sp|Q06147|LCB5_YEAST Sphingoid long chain base kinase 5 OS=Sacch... 86 2e-016
sp|Q12246|LCB4_YEAST Sphingoid long chain base kinase 4 OS=Sacch... 86 2e-016
sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Ara... 80 2e-014
sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium disc... 79 2e-014
sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium disc... 77 1e-013
sp|Q10123|YSM3_CAEEL Uncharacterized protein F52C9.3 OS=Caenorha... 54 1e-006
>sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SPHK2 PE=1 SV=2
Length = 654
Score = 103 bits (255), Expect = 2e-021
Identities = 48/100 (48%), Positives = 73/100 (73%)
Frame = +3
Query: 384 LVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDL 563
L+ RP RLL+ VNPFGG+ A + V P+ +A + ++ +T+ Q HA+E+V+ + L
Sbjct: 176 LLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSL 235
Query: 564 SKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
S++DGIV VSGDG+L EV+NGLL+R DW A+K+P+G++P
Sbjct: 236 SEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILP 275
>sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sphk2 PE=1 SV=2
Length = 617
Score = 101 bits (251), Expect = 6e-021
Identities = 46/100 (46%), Positives = 74/100 (74%)
Frame = +3
Query: 384 LVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDL 563
L+ R RLL+ VNPFGG+ A + ++ V P+ +A + ++ +T+ Q HA+E+V+ + L
Sbjct: 141 LLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSL 200
Query: 564 SKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
S+++GIV VSGDG+L EV+NGLL+R DW A+++PIG++P
Sbjct: 201 SEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLP 240
>sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1
Length = 537
Score = 100 bits (247), Expect = 2e-020
Identities = 47/97 (48%), Positives = 65/97 (67%)
Frame = +3
Query: 348 LWCDKLRQRLDSLVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQ 527
LW LR+ L+ L RPK LLVF+NPFGGK ++I+ +V PLF A + D+ T++
Sbjct: 114 LWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTLASITTDIIVTEHA 173
Query: 528 LHAKEMVRSIDLSKYDGIVCVSGDGVLVEVVNGLLER 638
AKE + I++ KYDGIVCV GDG+ EV++GL+ R
Sbjct: 174 NQAKETLYEINIDKYDGIVCVGGDGMFSEVLHGLIGR 210
>sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1 PE=1 SV=1
Length = 384
Score = 96 bits (238), Expect = 2e-019
Identities = 45/98 (45%), Positives = 69/98 (70%)
Frame = +3
Query: 393 RPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSKY 572
RP R+LV +NP GGK A ++F V+PL +A + + T+ + HA+E+VRS +L ++
Sbjct: 13 RPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRW 72
Query: 573 DGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVPA 686
D +V +SGDG++ EVVNGL+ER DW TA++ P+ +PA
Sbjct: 73 DALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPA 110
>sp|O14159|LCB4_SCHPO Sphingoid long chain base kinase 4 OS=Schizosaccharomyces
pombe GN=lcb4 PE=2 SV=1
Length = 458
Score = 96 bits (237), Expect = 2e-019
Identities = 47/116 (40%), Positives = 72/116 (62%)
Frame = +3
Query: 336 ESRKLWCDKLRQRLDSLVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQE 515
E+ +C+ L + R +R +VF+NP GGK A+ I+ E +P+F AH +V
Sbjct: 85 ENSDQFCEYLLDVAYKGIKRSRRFIVFINPHGGKGKAKHIWESEAEPVFSSAHSICEVVL 144
Query: 516 TKYQLHAKEMVRSIDLSKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
T+ + HAK + +++D+ YDGI+ V GDG+ EV+NGL ER D+ A KLP+ M+P
Sbjct: 145 TRRKDHAKSIAKNLDVGSYDGILSVGGDGLFHEVINGLGERDDYLEAFKLPVCMIP 200
>sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1
Length = 531
Score = 93 bits (229), Expect = 2e-018
Identities = 45/97 (46%), Positives = 63/97 (64%)
Frame = +3
Query: 348 LWCDKLRQRLDSLVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQ 527
LW LR L+SL RPK LLVF+NPFGGK ++I+ + V PLF A + ++ T++
Sbjct: 114 LWLQTLRGLLESLTSRPKHLLVFINPFGGKGQGKRIYEKTVAPLFTLASITTEIIITEHA 173
Query: 528 LHAKEMVRSIDLSKYDGIVCVSGDGVLVEVVNGLLER 638
AKE + I+ YDGIVCV GDG+ EV++G++ R
Sbjct: 174 NQAKETLYEINTDSYDGIVCVGGDGMFSEVLHGVIGR 210
>sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus GN=Sphk1 PE=1
SV=1
Length = 383
Score = 93 bits (229), Expect = 2e-018
Identities = 44/100 (44%), Positives = 68/100 (68%)
Frame = +3
Query: 384 LVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDL 563
L+ RP R+LV +NP GGK A K+F V+PL ++A V + T+ Q HA+E+V + +L
Sbjct: 10 LLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEEL 69
Query: 564 SKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
+D + +SGDG++ EVVNGL+ER DW +A++ P+ +P
Sbjct: 70 GHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLP 109
>sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sphk1 PE=1 SV=1
Length = 382
Score = 87 bits (215), Expect = 8e-017
Identities = 41/100 (41%), Positives = 67/100 (67%)
Frame = +3
Query: 384 LVGRPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDL 563
L+ RP R+LV +NP GGK A ++F V+P ++A + + T+ + HA+E+V + +L
Sbjct: 10 LLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEEL 69
Query: 564 SKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
+D + +SGDG++ EVVNGL+ER DW TA++ P+ +P
Sbjct: 70 GHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLP 109
>sp|Q06147|LCB5_YEAST Sphingoid long chain base kinase 5 OS=Saccharomyces
cerevisiae GN=LCB5 PE=1 SV=1
Length = 687
Score = 86 bits (212), Expect = 2e-016
Identities = 40/84 (47%), Positives = 55/84 (65%)
Frame = +3
Query: 393 RPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSKY 572
R K + V +NPFGGK A+K+F+ + KPL + ++V TKY HA E+ R +D+ KY
Sbjct: 267 RNKSIFVIINPFGGKGKAKKLFMTKAKPLLLASRCSIEVVYTKYPGHAIEIAREMDIDKY 326
Query: 573 DGIVCVSGDGVLVEVVNGLLERAD 644
D I C SGDG+ EV+NGL +R D
Sbjct: 327 DTIACASGDGIPHEVINGLYQRPD 350
>sp|Q12246|LCB4_YEAST Sphingoid long chain base kinase 4 OS=Saccharomyces
cerevisiae GN=LCB4 PE=1 SV=1
Length = 624
Score = 86 bits (211), Expect = 2e-016
Identities = 40/98 (40%), Positives = 63/98 (64%), Gaps = 1/98 (1%)
Frame = +3
Query: 393 RPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSKY 572
R + +LV +NP GGK TA+ +FL + +P+ ++ ++++ TKY HA ++ + +D+SKY
Sbjct: 225 RNRSILVIINPHGGKGTAKNLFLTKARPILVESGCKIEIAYTKYARHAIDIAKDLDISKY 284
Query: 573 DGIVCVSGDGVLVEVVNGLLERADWRTAL-KLPIGMVP 683
D I C SGDG+ EV+NGL R D A KL + +P
Sbjct: 285 DTIACASGDGIPYEVINGLYRRPDRVDAFNKLAVTQLP 322
>sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Arabidopsis
thaliana GN=LCBK1 PE=1 SV=1
Length = 763
Score = 80 bits (195), Expect = 2e-014
Identities = 38/96 (39%), Positives = 66/96 (68%), Gaps = 1/96 (1%)
Frame = +3
Query: 402 RLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSK-YDG 578
++LV +NP G + K+F V+P+F A ++++V +T HA+E+ ++D++ DG
Sbjct: 249 KMLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINLCSDG 308
Query: 579 IVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVPA 686
I+CV GDG++ EV+NGLL R++ + + +PIG+VPA
Sbjct: 309 IICVGGDGIINEVLNGLLTRSNPKEGVSIPIGIVPA 344
>sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium discoideum GN=sgkB
PE=2 SV=1
Length = 760
Score = 79 bits (194), Expect = 2e-014
Identities = 40/101 (39%), Positives = 65/101 (64%), Gaps = 3/101 (2%)
Frame = +3
Query: 390 GRPK--RLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDL 563
G PK ++ + +NP GKK + IF +EV+ LF D+ +++ + T HAK++ ++
Sbjct: 245 GNPKNRKIRILINPKSGKKESHNIF-KEVEQLFKDSGIKMKLTVTMEPEHAKKIGFKSNI 303
Query: 564 SKYDGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVPA 686
KYD +V +SGDG+L E +NGLL R D+ A K+P+ ++PA
Sbjct: 304 YKYDTVVFISGDGLLHEFINGLLSREDYEDAKKIPLALIPA 344
>sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium discoideum GN=sgkA
PE=2 SV=2
Length = 624
Score = 77 bits (187), Expect = 1e-013
Identities = 39/97 (40%), Positives = 60/97 (61%), Gaps = 1/97 (1%)
Frame = +3
Query: 393 RPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSKY 572
R +R+ V +NP GKK + IF +++ LF D+ + + TK HAK++ +L KY
Sbjct: 179 RERRIRVILNPKSGKKMSDSIF-KDINELFKDSKIFVKKTVTKGPDHAKKIGYKFNLKKY 237
Query: 573 DGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
D IV +SGDG+ E +NGLL R D+ A K+P+ ++P
Sbjct: 238 DTIVFISGDGLFHEFINGLLSRTDFEQARKIPLALIP 274
>sp|Q10123|YSM3_CAEEL Uncharacterized protein F52C9.3 OS=Caenorhabditis elegans
GN=F52C9.3 PE=2 SV=2
Length = 439
Score = 54 bits (127), Expect = 1e-006
Identities = 32/97 (32%), Positives = 47/97 (48%), Gaps = 3/97 (3%)
Frame = +3
Query: 393 RPKRLLVFVNPFGGKKTARKIFLEEVKPLFDDAHVQLDVQETKYQLHAKEMVRSIDLSKY 572
RPKR+ V VN G + F + PLF A VQ+DV + Q + + ++D +
Sbjct: 66 RPKRVFVLVNVEGNSRGCFDQFNKNALPLFHLAGVQVDVVKADNQAQLEALAGAVDTQEA 125
Query: 573 DGIVCVSGDGVLVEVVNGLLERADWRTALKLPIGMVP 683
D + V GDG + VV G+ R +LP+G P
Sbjct: 126 DILYVVGGDGTIGTVVTGIFRN---REKAQLPVGFYP 159
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 119,210,902,748
Number of Sequences: 518415
Number of Extensions: 119210902748
Number of Successful Extensions: 754584153
Number of sequences better than 0.0: 0
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