BLASTX 7.6.2
Query= UN33329 /QuerySize=950
(949 letters)
Database: GenBank nr;
15,229,318 sequences; 5,219,829,378 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|15218771|ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] 148 2e-033
gi|22655064|gb|AAM98123.1| putative isoamylase [Arabidopsis thal... 148 2e-033
gi|297843142|ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidops... 141 2e-031
gi|255584716|ref|XP_002533079.1| isoamylase, putative [Ricinus c... 88 2e-015
gi|73698627|gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum] 83 5e-014
gi|225436952|ref|XP_002271798.1| PREDICTED: hypothetical protein... 77 4e-012
gi|139867055|dbj|BAF52942.1| isoamylase-type starch-debranching ... 76 6e-012
gi|224149032|ref|XP_002336746.1| predicted protein [Populus tric... 70 3e-010
gi|41687960|dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare su... 64 3e-008
gi|229610875|emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. ... 64 3e-008
gi|326498607|dbj|BAK02289.1| predicted protein [Hordeum vulgare ... 64 3e-008
gi|294462564|gb|ADE76828.1| unknown [Picea sitchensis] 61 2e-007
gi|162463513|ref|NP_001105666.1| isoamylase-type starch debranch... 60 6e-007
gi|195657421|gb|ACG48178.1| isoamylase-type starch debranching e... 60 6e-007
gi|51038091|gb|AAT93894.1| putative isoamylase-type starch debra... 59 8e-007
gi|125551014|gb|EAY96723.1| hypothetical protein OsI_18641 [Oryz... 59 8e-007
gi|222630374|gb|EEE62506.1| hypothetical protein OsJ_17304 [Oryz... 59 8e-007
>gi|15218771|ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana]
Length = 882
Score = 148 bits (372), Expect = 2e-033
Identities = 100/152 (65%), Positives = 107/152 (70%), Gaps = 23/152 (15%)
Frame = -2
Query: 780 MAVWSPSARIGSCCLLNKDITETTWRFPSA-CFPTCKNKTKRGSETLR----------VT 634
MA WSPS IGSCC LN IT TW+FPSA F KNK K GSETL VT
Sbjct: 1 MAAWSPSVGIGSCC-LNNGITR-TWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58
Query: 633 SALQSYHHRLSKIRASKTSTELKEEVLSTRSAQVDDLKKKVTTTSYSFRTKSGALVKVKV 454
SALQSY + SKI ASKTS EL+ E LS+R A+ DDLKK TSYSFRTK+GALVKVKV
Sbjct: 59 SALQSY--QFSKICASKTSIELR-EALSSRRAEADDLKK---VTSYSFRTKAGALVKVKV 112
Query: 453 EKKREEKYSIMVYVSSLELS---SSSLVMVWG 367
EKKR EKYSI+VYVSSLELS S LVMVWG
Sbjct: 113 EKKR-EKYSILVYVSSLELSGDDKSRLVMVWG 143
Score = 141 bits (354), Expect = 2e-031
Identities = 70/87 (80%), Positives = 76/87 (87%), Gaps = 4/87 (4%)
Frame = -3
Query: 365 EPTEPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEE 186
EP EPK+NDLFIGFNASD PE+V+LPSLPDG +WRRLVDTALPFPGFFSVEGETVVA
Sbjct: 799 EPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVA--- 855
Query: 185 EEEMNQPVMYEMKPYSCTLFETTINAT 105
EE + Q V+YEMKPYSCTLFE TIN T
Sbjct: 856 EEPLQQLVVYEMKPYSCTLFE-TINTT 881
>gi|22655064|gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
Length = 882
Score = 148 bits (372), Expect = 2e-033
Identities = 100/152 (65%), Positives = 107/152 (70%), Gaps = 23/152 (15%)
Frame = -2
Query: 780 MAVWSPSARIGSCCLLNKDITETTWRFPSA-CFPTCKNKTKRGSETLR----------VT 634
MA WSPS IGSCC LN IT TW+FPSA F KNK K GSETL VT
Sbjct: 1 MAAWSPSVGIGSCC-LNNGITR-TWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVT 58
Query: 633 SALQSYHHRLSKIRASKTSTELKEEVLSTRSAQVDDLKKKVTTTSYSFRTKSGALVKVKV 454
SALQSY + SKI ASKTS EL+ E LS+R A+ DDLKK TSYSFRTK+GALVKVKV
Sbjct: 59 SALQSY--QFSKICASKTSIELR-EALSSRRAEADDLKK---VTSYSFRTKAGALVKVKV 112
Query: 453 EKKREEKYSIMVYVSSLELS---SSSLVMVWG 367
EKKR EKYSI+VYVSSLELS S LVMVWG
Sbjct: 113 EKKR-EKYSILVYVSSLELSGDDKSRLVMVWG 143
Score = 140 bits (351), Expect = 4e-031
Identities = 67/82 (81%), Positives = 73/82 (89%), Gaps = 3/82 (3%)
Frame = -3
Query: 365 EPTEPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEE 186
EP EPK+NDLFIGFNASD PE+V+LPSLPDG +WRRLVDTALPFPGFFSVEGETVVA
Sbjct: 799 EPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVA--- 855
Query: 185 EEEMNQPVMYEMKPYSCTLFET 120
EE + Q V+YEMKPYSCTLFET
Sbjct: 856 EEPLQQLVVYEMKPYSCTLFET 877
>gi|297843142|ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata
subsp. lyrata]
Length = 872
Score = 141 bits (355), Expect = 2e-031
Identities = 67/83 (80%), Positives = 75/83 (90%), Gaps = 2/83 (2%)
Frame = -3
Query: 368 VEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEE 189
VEPTEPK+NDLFIGFNASD PE+VILPSLPDG +WRRLVDTALPFPGFFSVEGETVV
Sbjct: 787 VEPTEPKSNDLFIGFNASDHPESVILPSLPDGSKWRRLVDTALPFPGFFSVEGETVVV-- 844
Query: 188 EEEEMNQPVMYEMKPYSCTLFET 120
EE++ Q ++YEM+PYSCTLFET
Sbjct: 845 AEEQLQQLIVYEMEPYSCTLFET 867
Score = 133 bits (334), Expect = 4e-029
Identities = 88/128 (68%), Positives = 95/128 (74%), Gaps = 20/128 (15%)
Frame = -2
Query: 711 TWRFPSACFPTCKNKTKRGSETLR----------VTSALQSYHHRLSKIRASKTSTELKE 562
TW+FPSA F T KNK K GSETL VTSAL+SY +LSKI ASKTS EL+
Sbjct: 12 TWKFPSARFFTGKNKIKLGSETLMFTRKRFMGDLVTSALKSY--QLSKICASKTSIELR- 68
Query: 561 EVLSTRSAQVDDLKKKVTTTSYSFRTKSGALVKVKVEKKREEKYSIMVYVSSLELS---S 391
E LST+ AQVDDLKK TSYSFRTKSGALVKVKVE+KR EKYSIMVYVSSLEL+
Sbjct: 69 EALSTKRAQVDDLKK---VTSYSFRTKSGALVKVKVEQKR-EKYSIMVYVSSLELTGDDK 124
Query: 390 SSLVMVWG 367
S LVMVWG
Sbjct: 125 SRLVMVWG 132
>gi|255584716|ref|XP_002533079.1| isoamylase, putative [Ricinus communis]
Length = 872
Score = 88 bits (216), Expect = 2e-015
Identities = 45/81 (55%), Positives = 57/81 (70%), Gaps = 7/81 (8%)
Frame = -3
Query: 359 TEPKN--NDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEE 186
+EP N DLF+ FNA+ E+VILP +P+G WRRLVDTALPFPGFFS +GE VV
Sbjct: 791 SEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFPGFFSEDGEPVV---- 846
Query: 185 EEEMNQPVMYEMKPYSCTLFE 123
E++ + Y+M +SCTLFE
Sbjct: 847 -EQIAGLIAYKMNSHSCTLFE 866
>gi|73698627|gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
Length = 857
Score = 83 bits (204), Expect = 5e-014
Identities = 40/73 (54%), Positives = 49/73 (67%), Gaps = 5/73 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DLF+ FNA D PE V+LP P+G W R++DTALPFPGFF G+ V+ E+M+
Sbjct: 784 DLFVAFNADDCPETVVLPLPPEGMSWYRIIDTALPFPGFFLNNGDLVL-----EQMSGLS 838
Query: 161 MYEMKPYSCTLFE 123
YEMK YSC LFE
Sbjct: 839 TYEMKSYSCILFE 851
>gi|225436952|ref|XP_002271798.1| PREDICTED: hypothetical protein [Vitis
vinifera]
Length = 882
Score = 77 bits (188), Expect = 4e-012
Identities = 40/85 (47%), Positives = 54/85 (63%), Gaps = 7/85 (8%)
Frame = -3
Query: 371 GVEPTEPKN--NDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVV 198
G P+E + DLFI FN +D+ VILP P G W RLVDTALPFPGFF+ +GE ++
Sbjct: 797 GQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLVDTALPFPGFFTADGEAIL 856
Query: 197 AEEEEEEMNQPVMYEMKPYSCTLFE 123
++ + V Y+M+ +SC LFE
Sbjct: 857 -----KKKSGLVTYKMESHSCALFE 876
>gi|139867055|dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2
[Phaseolus vulgaris]
Length = 865
Score = 76 bits (186), Expect = 6e-012
Identities = 38/76 (50%), Positives = 49/76 (64%), Gaps = 7/76 (9%)
Frame = -3
Query: 347 NNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQ 168
+ D+FI N +D+PE+ +LP P+G W RLVDT+LPFPGFFS GE V EM
Sbjct: 790 SGDIFIALNVADEPESTVLPLPPEGMSWYRLVDTSLPFPGFFSSSGEIV------PEMKA 843
Query: 167 PV-MYEMKPYSCTLFE 123
+ Y+MK +SC LFE
Sbjct: 844 GLSTYKMKSHSCALFE 859
>gi|224149032|ref|XP_002336746.1| predicted protein [Populus trichocarpa]
Length = 283
Score = 70 bits (171), Expect = 3e-010
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
D+FI FNA+ + E+V LP +P+G W RLVDTALPFPGFFS + E V+ + E + + +
Sbjct: 215 DMFIAFNAAGRSESVTLPEVPEGMAWHRLVDTALPFPGFFSNDSEPVIRQPYEMKSHSCI 274
Query: 161 MYE 153
+ E
Sbjct: 275 LLE 277
>gi|41687960|dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 64 bits (154), Expect = 3e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = -3
Query: 356 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 177
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 176 MNQPVMYEMKPYSCTLFET 120
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|229610875|emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 64 bits (154), Expect = 3e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = -3
Query: 356 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 177
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 176 MNQPVMYEMKPYSCTLFET 120
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|326498607|dbj|BAK02289.1| predicted protein [Hordeum vulgare subsp.
vulgare]
Length = 798
Score = 64 bits (154), Expect = 3e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = -3
Query: 356 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 177
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 176 MNQPVMYEMKPYSCTLFET 120
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|294462564|gb|ADE76828.1| unknown [Picea sitchensis]
Length = 175
Score = 61 bits (147), Expect = 2e-007
Identities = 32/73 (43%), Positives = 42/73 (57%), Gaps = 5/73 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FN+ + LP LPDG W RLVDT+LPFP FS+EG +
Sbjct: 102 DLYIAFNSQKASVSATLPQLPDGMLWHRLVDTSLPFPKTFSMEGMAT-----DMLGISFS 156
Query: 161 MYEMKPYSCTLFE 123
Y+++P+SC LFE
Sbjct: 157 TYQIQPHSCVLFE 169
>gi|162463513|ref|NP_001105666.1| isoamylase-type starch debranching enzyme ISO2
[Zea mays]
Length = 799
Score = 60 bits (143), Expect = 6e-007
Identities = 30/74 (40%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FNA+++ + LP++ +G W RLVDT+L FPGFFS + E ++
Sbjct: 726 DLYICFNANEESASATLPAIAEGSMWLRLVDTSLAFPGFFSRG-----SSHETHQVLGFS 780
Query: 161 MYEMKPYSCTLFET 120
Y++K +SC LFE+
Sbjct: 781 SYQVKAHSCVLFES 794
>gi|195657421|gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Zea
mays]
Length = 799
Score = 60 bits (143), Expect = 6e-007
Identities = 30/74 (40%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FNA+++ + LP++ +G W RLVDT+L FPGFFS + E ++
Sbjct: 726 DLYICFNANEESASATLPAIAEGSMWLRLVDTSLAFPGFFSRG-----SSHETHQVLGFS 780
Query: 161 MYEMKPYSCTLFET 120
Y++K +SC LFE+
Sbjct: 781 SYQVKAHSCVLFES 794
>gi|51038091|gb|AAT93894.1| putative isoamylase-type starch debranching enzyme
ISO2 [Oryza sativa Japonica Group]
Length = 800
Score = 59 bits (142), Expect = 8e-007
Identities = 29/74 (39%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FNA+++ + LP+L +G W RLVDT+L FPGFF+ E V +++
Sbjct: 727 DLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKV-----QQVPGLS 781
Query: 161 MYEMKPYSCTLFET 120
Y ++ ++C LFE+
Sbjct: 782 SYHVEAHTCVLFES 795
>gi|125551014|gb|EAY96723.1| hypothetical protein OsI_18641 [Oryza sativa Indica
Group]
Length = 840
Score = 59 bits (142), Expect = 8e-007
Identities = 29/74 (39%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FNA+++ + LP+L +G W RLVDT+L FPGFF+ E V +++
Sbjct: 767 DLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKV-----QQVPGLS 821
Query: 161 MYEMKPYSCTLFET 120
Y ++ ++C LFE+
Sbjct: 822 SYHVEAHTCVLFES 835
>gi|222630374|gb|EEE62506.1| hypothetical protein OsJ_17304 [Oryza sativa
Japonica Group]
Length = 541
Score = 59 bits (142), Expect = 8e-007
Identities = 29/74 (39%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = -3
Query: 341 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 162
DL+I FNA+++ + LP+L +G W RLVDT+L FPGFF+ E V +++
Sbjct: 468 DLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKV-----QQVPGLS 522
Query: 161 MYEMKPYSCTLFET 120
Y ++ ++C LFE+
Sbjct: 523 SYHVEAHTCVLFES 536
Database: GenBank nr
Posted date: Thu Sep 08 23:06:31 2011
Number of letters in database: 5,219,829,378
Number of sequences in database: 15,229,318
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,810,539,025,462
Number of Sequences: 15229318
Number of Extensions: 3810539025462
Number of Successful Extensions: 885398663
Number of sequences better than 0.0: 0
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